BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0599.0004.p (299 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gb|EAJ16229.1| unknown [environmental sequence] 93 9e-18 gb|AAR37531.1| oxidoreductase, short-chain dehydrogenase/reducta... 89 2e-16 gb|EAJ09825.1| unknown [environmental sequence] 87 5e-16 ref|ZP_00019884.1| hypothetical protein [Chloroflexus aurantiacus] 84 4e-15 ref|NP_961304.1| hypothetical protein MAP2370c [Mycobacterium av... 82 1e-14 >gb|EAJ16229.1| unknown [environmental sequence] Length = 288 Score = 92.8 bits (229), Expect = 9e-18, Method: Compositional matrix adjust. Identities = 69/258 (26%), Positives = 128/258 (49%), Gaps = 3/258 (1%) Query: 14 LNTNQREHIALITGASQGIGLALARKLLSQNWQVITLNRSDFPADDMLIQNALKNGWLRA 73 +N N + LITGA+ GIG A++L ++I + R+ + + Q ++G Sbjct: 3 MNENLENKVCLITGATNGIGEESAKELNKMGAEIIFIARNPEKGNRLKEQLLKESGREAT 62 Query: 74 YKVKDLTDYGSLRRSLEEIKSKEQRIDILFNNAGGGLSELRYSKQGREMHYELLTVVPYI 133 V DL+ ++++ E S E+ +DIL NNAG E + + G E + + + + Sbjct: 63 MIVADLSSQADVKQAAEAFLSMEKPLDILLNNAGIMNRERKETIDGLEEVFSVNHLAYFT 122 Query: 134 ILMELKELLKNGSLKTVINTSSQVLRFTKEFSIENLEHPKTFRKMYGPYATSKLALSLWT 193 + L E LKN S V+N +S +F K+ + E+L+ KTFR M Y SKLA L+T Sbjct: 123 FTLLLAEKLKNTSGSRVVNVASGAHQFVKDMNFEDLQSEKTFRPMQ-VYGQSKLANILFT 181 Query: 194 QAVAPQLAKEGIKIRSVDPGINNT-LRKGKDSGLTAWFELFMKVFSSPPTHGANL-LFEG 251 ++++ +L+ G+ + + PG +T + + GL + + F+ GA ++ Sbjct: 182 KSLSEKLSSFGVTVNCLHPGFVSTGIGSNNNQGLWPFLMFLARPFARKTDKGAETSIYLC 241 Query: 252 ALGKHRNETGVFLLKDRV 269 + ++ TG + + ++ Sbjct: 242 CSSEVKDTTGEYFVDCKI 259 >gb|AAR37531.1| oxidoreductase, short-chain dehydrogenase/reductase family [uncultured bacterium 311] Length = 285 Score = 88.6 bits (218), Expect = 2e-16, Method: Compositional matrix adjust. Identities = 65/227 (28%), Positives = 114/227 (50%), Gaps = 6/227 (2%) Query: 23 ALITGASQGIGLALARKLLSQNWQVITLNRSDFPADDMLIQNALKNGWLRAYKVKDLTDY 82 LITGA+ GIG + A L + + + R+ A+++ + +G + DL+ + Sbjct: 9 CLITGATSGIGRSTALALSEKGANIFFIARNQQKAEELTEEVERVSGKSPNAIIADLSSF 68 Query: 83 GSLRRSLEEIKSKEQRIDILFNNAGGGLSELRYSKQGREMHYELLTVVPYIILMELKELL 142 + R+ EE KS + ID+L NNAG +E R ++ G E + + + +++ L E + Sbjct: 69 KQIERAAEEFKSLNKPIDVLLNNAGIMNTERRVTEDGLEEVFAVNHLAYFLLTNLLIEKI 128 Query: 143 KNGSLKTVINTSSQVLRFTKEFSIENLEHPKTFRKMYGPYATSKLALSLWTQAVAPQLAK 202 LK V+N SS RF K + ++L+ K F KM+ Y SKLA L+T+ ++ + Sbjct: 129 LESGLKRVVNVSSDAHRFLKSMNFDDLQSEKEF-KMFAAYGQSKLANILFTRKLSSLYQE 187 Query: 203 EGIKIRSVDPGINNTLRKGKDSGLTAWFELFMK----VFSSPPTHGA 245 EG+ + PG +T ++ L A+F ++ + + P GA Sbjct: 188 EGLTTNCLHPGFVSTSIGAQNENL-AFFARLIRWVSPLIAKPSDKGA 233 >gb|EAJ09825.1| unknown [environmental sequence] Length = 287 Score = 87.0 bits (214), Expect = 5e-16, Method: Compositional matrix adjust. Identities = 72/254 (28%), Positives = 121/254 (47%), Gaps = 12/254 (4%) Query: 24 LITGASQGIGLALARKLLSQNWQVITLNRSDFPADDM------LIQNALKNGWLRAYKVK 77 LITGA+ GIG A A K + R++ A+D+ + N NG+ + V+ Sbjct: 10 LITGATNGIGKAAAIKFAESAKSIAFTYRNEELAEDLKNEMQKINPNLSINGFFCDFSVQ 69 Query: 78 DLTDYGSLRRSLEEIKSKEQRIDILFNNAGGGLSELRYSKQGREMHYELLTVVPYIILME 137 D S+R +IK+ + ID+L NNAG +E + G E + + + ++ Sbjct: 70 D-----SIRECANKIKNDLKAIDLLINNAGVVNTEYSETIDGIENTFAVNHLGYFLFTNL 124 Query: 138 LKELLKNGSLKTVINTSSQVLRFTKEFSIENLEHPKTFRKMYGPYATSKLALSLWTQAVA 197 L +L+K S +IN SS F K +++ + F+ Y SKL L+T+ +A Sbjct: 125 LLDLVKKESGSRIINVSSAAHHFVKGMQWDDINYKDDFKMGLKAYGQSKLGNILFTKQLA 184 Query: 198 PQLAKEGIKIRSVDP-GINNTLRKGKDSGLTAWFELFMKVFSSPPTHGANLLFEGALGKH 256 +L K+G+ + ++ P G+N +L +S L ++ +K F P GAN + A Sbjct: 185 RKLQKDGVTVNAIHPGGVNTSLGNQNNSLLGRVLKIILKPFFRSPLKGANTIIYLAEIDG 244 Query: 257 RNETGVFLLKDRVA 270 + TG + + RVA Sbjct: 245 LSITGAYWVDGRVA 258 >ref|ZP_00019884.1| hypothetical protein [Chloroflexus aurantiacus] Length = 292 Score = 83.6 bits (205), Expect = 4e-15, Method: Compositional matrix adjust. Identities = 67/225 (29%), Positives = 104/225 (46%), Gaps = 6/225 (2%) Query: 24 LITGASQGIGLALARKLLSQNWQVITLNRSDF---PADDMLIQNALKNGWLRAYKVKDLT 80 ++TGA+ GIG AR+L + +VI + RS A ++Q A K + D Sbjct: 16 IVTGANSGIGYVTARELAAMGARVIMVCRSQSKGEAARQRIMQEA-KGAPEPELVLADFA 74 Query: 81 DYGSLRRSLEEIKSKEQRIDILFNNAGGGLSELRYSKQGREMHYELLTVVPYIILMELKE 140 S+RR+ +I + RID+L NNAG +SE S G EM + + + P+++ L E Sbjct: 75 SLASVRRAAGDILERCPRIDVLVNNAGLFVSEPLASADGYEMTFAVNHLAPFLLTNLLLE 134 Query: 141 LLKNGSLKTVINTSSQVLRFTKEFSIENLEHPKTFRKMYGPYATSKLALSLWTQAVAPQL 200 + + +IN SS +I + P+ + Y+ SKL L+T +A +L Sbjct: 135 RIIASAPARIINVSS-FAHVAGRIAIPQIASPQR-PNIAQAYSDSKLCNILFTNELARRL 192 Query: 201 AKEGIKIRSVDPGINNTLRKGKDSGLTAWFELFMKVFSSPPTHGA 245 G+ S+ PG T GL A+F + F P HGA Sbjct: 193 QGSGVTANSLHPGAVATNFAADSRGLFAFFFRLARPFMLSPEHGA 237 >ref|NP_961304.1| hypothetical protein MAP2370c [Mycobacterium avium subsp. paratuberculosis str. k10] gb|AAS04687.1| hypothetical protein MAP2370c [Mycobacterium avium subsp. paratuberculosis str. k10] Length = 274 Score = 82.4 bits (202), Expect = 1e-14, Method: Compositional matrix adjust. Identities = 75/256 (29%), Positives = 131/256 (51%), Gaps = 23/256 (8%) Query: 24 LITGASQGIGLALARKLLSQNWQVITLNRSDFPADDMLIQNALKNGWLRA-YKVKDLTDY 82 +ITGAS GIG A AR+L +++ + RS + A L A + V D D Sbjct: 7 VITGASDGIGAAAARRLSRAGDRIVVVGRSQ-------TKTAAVAAELGADHFVVDYADL 59 Query: 83 GSLRRSLEEIKSKEQRIDILFNNAGGGLSELRYSKQGREMHYELLTVVPYIILMELKELL 142 +R ++++++ RID+L NNAGG S + + G E Y++ + P+++ +L ++L Sbjct: 60 SQVRALADKMRAQYPRIDVLLNNAGGVASRIELTADGYERTYQVNYLAPFLLTTQLLDVL 119 Query: 143 KNGSLKTVINTSSQVLRFTKEFSIENLEHPKTFRKMYGPYATSKLALSLWTQAVAPQLAK 202 S TV+NT+S + ++++LE+ R YA SKLA+ L+T+ + + Sbjct: 120 LE-SRATVVNTTSSSHKLILRATVDDLENTANRRPAVA-YAYSKLAIVLFTKELHRRYHA 177 Query: 203 EGIKIRSVDPG-INNTLRKGKDSGLTAWFELFMK------VFSSPPTHGANLLFEGALGK 255 G+ + +V PG +N+ + G SG + F +FM+ +F S P GA+ L A Sbjct: 178 RGLSVAAVHPGNVNSNI--GIASG--SRFLVFMQRYTPAALFISSPDQGADPLVRLASSP 233 Query: 256 HRNE--TGVFLLKDRV 269 +E +G + K ++ Sbjct: 234 PDSEWTSGAYYAKRKI 249 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.318 0.136 0.388 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 434,244,867 Number of extensions: 17460273 Number of successful extensions: 65185 Number of sequences better than 1.0e-05: 612 Number of HSP's gapped: 66638 Number of HSP's successfully gapped: 612 Length of query: 299 Length of database: 757,237,578 Length adjustment: 125 Effective length of query: 174 Effective length of database: 417,850,203 Effective search space: 72705935322 Effective search space used: 72705935322 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 125 (52.8 bits)