BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0598.0010.p (127 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_00118090.1| COG0262: Dihydrofolate reductase [Cytophaga h... 107 1e-22 gb|AAS69280.1| conserved hypothetical protein [Leptospira interr... 104 5e-22 ref|NP_713718.1| conserved hypothetical protein [Leptospira inte... 103 1e-21 ref|NP_103938.1| hypothetical protein mlr2644 [Mesorhizobium lot... 100 1e-20 ref|ZP_00116969.1| COG0262: Dihydrofolate reductase [Cytophaga h... 99 3e-20 >ref|ZP_00118090.1| COG0262: Dihydrofolate reductase [Cytophaga hutchinsonii] Length = 182 Score = 107 bits (266), Expect = 1e-22, Method: Compositional matrix adjust. Identities = 50/127 (39%), Positives = 82/127 (64%), Gaps = 1/127 (0%) Query: 1 VLSNPSASQHERNHAEWIEKTAKIVFSTTLENVEWNNSRLVKEGVEEEINNLKQQPGEDM 60 + +NP+ + A I++ K+VFS TL++ WN++ L K +EEE+ LKQQPG+D+ Sbjct: 57 IRANPTGKKATDEFAAAIDRVPKLVFSHTLKDTGWNSATLAKGSLEEELTALKQQPGKDI 116 Query: 61 VILGSPRFAHHLMQLDLIDEYKITVSPVLIGKGLPLFQGLKEKINLKLIENKTFDSGAIG 120 + SP M L+LIDE+++ V PV+ G GLPLF+ +++K +L++ K F SGA+ Sbjct: 117 YVC-SPGMIVAAMNLNLIDEFQLCVHPVVAGSGLPLFKNIQDKPMFRLVQTKNFTSGAVI 175 Query: 121 LVYQTVR 127 L Y+ ++ Sbjct: 176 LYYEPLK 182 >gb|AAS69280.1| conserved hypothetical protein [Leptospira interrogans serovar Copenhageni str. Fiocruz L1-130] Length = 182 Score = 104 bits (260), Expect = 5e-22, Method: Compositional matrix adjust. Identities = 54/120 (45%), Positives = 79/120 (65%) Query: 5 PSASQHERNHAEWIEKTAKIVFSTTLENVEWNNSRLVKEGVEEEINNLKQQPGEDMVILG 64 P+A A + + KIVFS TL NV+W NS LV V++E+ LK+Q G+D+ I G Sbjct: 61 PTAIVDNPIIATKMNEITKIVFSKTLANVKWKNSILVYNNVKKEVAKLKKQQGKDIAIFG 120 Query: 65 SPRFAHHLMQLDLIDEYKITVSPVLIGKGLPLFQGLKEKINLKLIENKTFDSGAIGLVYQ 124 S A L++ LIDE +I ++P+++G+G LFQG++ +I+LKLI+ KTF SG + L YQ Sbjct: 121 SSSLAATLIEQKLIDEVRIFINPIVLGEGKTLFQGIQNRISLKLIQTKTFQSGNVLLYYQ 180 >ref|NP_713718.1| conserved hypothetical protein [Leptospira interrogans serovar lai str. 56601] gb|AAN50736.1| conserved hypothetical protein [Leptospira interrogans serovar lai str. 56601] Length = 182 Score = 103 bits (257), Expect = 1e-21, Method: Compositional matrix adjust. Identities = 54/120 (45%), Positives = 78/120 (65%) Query: 5 PSASQHERNHAEWIEKTAKIVFSTTLENVEWNNSRLVKEGVEEEINNLKQQPGEDMVILG 64 P+A A + + KIVFS TL NV+W NS LV V++E+ LK+Q G+D+ I G Sbjct: 61 PTAIVDNPIIATKMNEITKIVFSKTLANVKWKNSILVYNNVKKEVAKLKKQQGKDIAIFG 120 Query: 65 SPRFAHHLMQLDLIDEYKITVSPVLIGKGLPLFQGLKEKINLKLIENKTFDSGAIGLVYQ 124 S L++ LIDE +I ++PV++G+G LFQG++ +I+LKLI+ KTF SG + L YQ Sbjct: 121 SSSLVATLIEQKLIDEVRIFINPVVLGEGKTLFQGIQNRISLKLIQTKTFQSGNVLLYYQ 180 >ref|NP_103938.1| hypothetical protein mlr2644 [Mesorhizobium loti MAFF303099] dbj|BAB49724.1| hypothetical protein [Mesorhizobium loti] Length = 189 Score = 100 bits (248), Expect = 1e-20, Method: Compositional matrix adjust. Identities = 48/120 (40%), Positives = 73/120 (60%) Query: 5 PSASQHERNHAEWIEKTAKIVFSTTLENVEWNNSRLVKEGVEEEINNLKQQPGEDMVILG 64 P A+Q AE + K+VFS TL+ V WNN+ LVK G ++ LKQQPG M ILG Sbjct: 64 PMAAQIAPVVAERMNAMPKVVFSRTLDTVSWNNTELVKSGPAAKLRELKQQPGNHMTILG 123 Query: 65 SPRFAHHLMQLDLIDEYKITVSPVLIGKGLPLFQGLKEKINLKLIENKTFDSGAIGLVYQ 124 S L + +LIDEY+ + P+++G G +F+G+ ++N+KL ++ F +G + L Y+ Sbjct: 124 SGNLVAQLAEENLIDEYQFVIVPIMLGAGRTMFEGIGNRLNVKLARSQAFGNGNVFLRYE 183 >ref|ZP_00116969.1| COG0262: Dihydrofolate reductase [Cytophaga hutchinsonii] Length = 186 Score = 99.0 bits (245), Expect = 3e-20, Method: Compositional matrix adjust. Identities = 50/124 (40%), Positives = 79/124 (63%), Gaps = 1/124 (0%) Query: 1 VLSNPSASQHERNHAEWIEKTAKIVFSTTLENVEWNNSRLVKEGVEEEINNLKQQPGEDM 60 ++ P+ + A I+ KIVFS TL+ + W NS L K ++ EI LKQQPG++ Sbjct: 58 IVKEPTGVKAIDEFAILIDTIHKIVFSHTLKGLTWKNSTLAKGELKAEIIRLKQQPGKN- 116 Query: 61 VILGSPRFAHHLMQLDLIDEYKITVSPVLIGKGLPLFQGLKEKINLKLIENKTFDSGAIG 120 +++GSP L Q LIDEY++ + P+++GKGL LF+ ++++I+LK I +T+ SGAI Sbjct: 117 ILVGSPSLIVALTQFGLIDEYQLCIQPIILGKGLILFKHMQDRIDLKCIRTETYASGAIL 176 Query: 121 LVYQ 124 Y+ Sbjct: 177 ARYE 180 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.315 0.134 0.379 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 198,687,395 Number of extensions: 7968597 Number of successful extensions: 16244 Number of sequences better than 1.0e-05: 40 Number of HSP's gapped: 16243 Number of HSP's successfully gapped: 40 Length of query: 127 Length of database: 757,237,578 Length adjustment: 90 Effective length of query: 37 Effective length of database: 512,878,668 Effective search space: 18976510716 Effective search space used: 18976510716 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 120 (50.8 bits)