BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0596.0025.p (322 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gb|AAG49421.1| alpha-galactosidase AgaB [Geobacillus stearotherm... 437 e-121 gb|AAD23585.1| alpha-galactosidase AgaN [Geobacillus stearotherm... 436 e-121 gb|AAG49420.1| alpha-galactosidase AgaA [Geobacillus stearotherm... 436 e-121 emb|CAA69852.1| melibiase; alpha-galactosidase [Thermoanaerobact... 402 e-111 ref|NP_561428.1| alpha-galactosidase [Clostridium perfringens] >... 374 e-102 >gb|AAG49421.1| alpha-galactosidase AgaB [Geobacillus stearothermophilus] Length = 729 Score = 437 bits (1124), Expect = e-121, Method: Compositional matrix adjust. Identities = 207/318 (65%), Positives = 251/318 (78%), Gaps = 5/318 (1%) Query: 1 MISPDSELYRKHPDWCLHVPERRRTEARWQLVLDYTRQEVRDFIYDSLSAIFSSVPVAYV 60 M+SP+SELYRKHPDWCLHVP R R+E R QLVLDY+R++V D+I +++S + +S P+ YV Sbjct: 416 MVSPNSELYRKHPDWCLHVPNRPRSEGRNQLVLDYSREDVCDYIIETISNVLASAPITYV 475 Query: 61 KWDMNRCLTEIGSADLPPERQPETAHRYVLGLYELLERITSAFPHILFESCSSGGGRFDP 120 KWDMNR +TEIGS+ LPPERQ ETAHRY+LGLY +++ +TS FPHILFESCS GGGRFDP Sbjct: 476 KWDMNRHMTEIGSSALPPERQRETAHRYMLGLYRVMDEMTSRFPHILFESCSGGGGRFDP 535 Query: 121 GMLYYMPQTWTSDNTDAVERLKIQYGTSLVYPVSAIGAHVSAVPNHQVGRITPLDFRGDV 180 GMLYYMPQTWTSDNTDAV RLKIQYGTSLVYP+SA+GAHVSAVPNHQVGR+ L RG V Sbjct: 536 GMLYYMPQTWTSDNTDAVSRLKIQYGTSLVYPISAMGAHVSAVPNHQVGRVASLKARGHV 595 Query: 181 AMSGNFGYELDLTKFTEEDKETAKRQIITYKEIRGLVQRGDLYRLKSPFEGNEPAWMFVS 240 AMSGNFGYELD+TK TE +K+ K+Q+ YK++R LVQ G YRL SPFEGNE AWMFVS Sbjct: 596 AMSGNFGYELDITKLTETEKQMIKQQVAFYKDVRRLVQFGTFYRLLSPFEGNEAAWMFVS 655 Query: 241 EDKEEALVYHFQVMAVPNAPQRFLRLAGLHPELEYEIHSEYSGEQFICGGDRLMQLGLPL 300 D+ EALV +F+V+A NAP +LRL GL P +YEI + GGD LM G+ L Sbjct: 656 ADRSEALVAYFRVLAEANAPLSYLRLKGLDPNQDYEIEG-----LGVYGGDELMYAGVAL 710 Query: 301 VYEQKDYESGWFRVRAIK 318 Y D+ S +R++A++ Sbjct: 711 PYRSGDFISMMWRLKAVQ 728 >gb|AAD23585.1| alpha-galactosidase AgaN [Geobacillus stearothermophilus] Length = 729 Score = 436 bits (1122), Expect = e-121, Method: Compositional matrix adjust. Identities = 213/318 (66%), Positives = 252/318 (79%), Gaps = 4/318 (1%) Query: 1 MISPDSELYRKHPDWCLHVPERRRTEARWQLVLDYTRQEVRDFIYDSLSAIFSSVPVAYV 60 M+S DSELYR+HPDWCLHVP R R+E R QLVLDY+RQEV D+I +S + +S P++YV Sbjct: 416 MVSVDSELYRQHPDWCLHVPNRPRSEGRNQLVLDYSRQEVCDYIIQVISDVLASAPISYV 475 Query: 61 KWDMNRCLTEIGSADLPPERQPETAHRYVLGLYELLERITSAFPHILFESCSSGGGRFDP 120 KWDMNR +TEIGSA LPPERQ ETAHRY+LGLY ++E ITS FPH+LFESCS GGGRFDP Sbjct: 476 KWDMNRHMTEIGSAPLPPERQRETAHRYMLGLYRVMEEITSRFPHVLFESCSGGGGRFDP 535 Query: 121 GMLYYMPQTWTSDNTDAVERLKIQYGTSLVYPVSAIGAHVSAVPNHQVGRITPLDFRGDV 180 GMLYYMPQTWTSDNTDAV RLKIQYGTSLVYP+ ++GAHVSAVPNHQV RIT L+ RG V Sbjct: 536 GMLYYMPQTWTSDNTDAVSRLKIQYGTSLVYPIISMGAHVSAVPNHQVHRITSLEMRGHV 595 Query: 181 AMSGNFGYELDLTKFTEEDKETAKRQIITYKEIRGLVQRGDLYRLKSPFEGNEPAWMFVS 240 AMSGNFGYELDLTK +E++K+ K Q+ YKEIR LVQ G YR+ SPFEGNE AWMFVS Sbjct: 596 AMSGNFGYELDLTKLSEKEKQKVKEQVAFYKEIRRLVQFGTFYRILSPFEGNEAAWMFVS 655 Query: 241 EDKEEALVYHFQVMAVPNAPQRFLRLAGLHPELEYEIHSEYSGEQFICGGDRLMQLGLPL 300 ED+ EALV +F+V+A NAP F+RL GL P +YEI SGE + GGD LM GL + Sbjct: 656 EDQSEALVAYFRVLAEANAPLSFIRLKGLDPNKDYEIIG--SGE--VYGGDELMYAGLNV 711 Query: 301 VYEQKDYESGWFRVRAIK 318 + D+ S +R++A + Sbjct: 712 PQRRGDFISVMWRLKAAR 729 >gb|AAG49420.1| alpha-galactosidase AgaA [Geobacillus stearothermophilus] Length = 729 Score = 436 bits (1122), Expect = e-121, Method: Compositional matrix adjust. Identities = 207/318 (65%), Positives = 250/318 (78%), Gaps = 5/318 (1%) Query: 1 MISPDSELYRKHPDWCLHVPERRRTEARWQLVLDYTRQEVRDFIYDSLSAIFSSVPVAYV 60 M+SP+SELYRKHPDWCLHVP R R+E R QLVLDY+R++V D+I +++S + +S P+ YV Sbjct: 416 MVSPNSELYRKHPDWCLHVPNRPRSEGRNQLVLDYSREDVCDYIIETISNVLASAPITYV 475 Query: 61 KWDMNRCLTEIGSADLPPERQPETAHRYVLGLYELLERITSAFPHILFESCSSGGGRFDP 120 KWDMNR +TEIGS+ LPPERQ ETAHRY+LGLY +++ ITS FPHILFESCS GGGRFDP Sbjct: 476 KWDMNRHMTEIGSSALPPERQRETAHRYMLGLYRVMDEITSRFPHILFESCSGGGGRFDP 535 Query: 121 GMLYYMPQTWTSDNTDAVERLKIQYGTSLVYPVSAIGAHVSAVPNHQVGRITPLDFRGDV 180 GMLYYMPQTWTSDNTDAV RLKIQYGTSLVYP+SA+GAHVSAVPNHQVGR+ L RG V Sbjct: 536 GMLYYMPQTWTSDNTDAVSRLKIQYGTSLVYPISAMGAHVSAVPNHQVGRVASLKTRGHV 595 Query: 181 AMSGNFGYELDLTKFTEEDKETAKRQIITYKEIRGLVQRGDLYRLKSPFEGNEPAWMFVS 240 AMSGNFGYELD+TK TE +K+ K+Q+ YK++R LVQ G YRL SPFEGNE AWMFVS Sbjct: 596 AMSGNFGYELDITKLTETEKQMMKQQVAFYKDVRRLVQFGTFYRLLSPFEGNEAAWMFVS 655 Query: 241 EDKEEALVYHFQVMAVPNAPQRFLRLAGLHPELEYEIHSEYSGEQFICGGDRLMQLGLPL 300 D+ EALV +F+V+A NAP +LRL GL +YEI + GGD LM G+ L Sbjct: 656 ADRSEALVAYFRVLAEANAPLSYLRLKGLDSNQDYEIEG-----LGVYGGDELMYAGVAL 710 Query: 301 VYEQKDYESGWFRVRAIK 318 Y D+ S +R++A++ Sbjct: 711 PYRSSDFISMMWRLKAVQ 728 >emb|CAA69852.1| melibiase; alpha-galactosidase [Thermoanaerobacter ethanolicus] Length = 626 Score = 402 bits (1034), Expect = e-111, Method: Compositional matrix adjust. Identities = 189/309 (61%), Positives = 243/309 (78%), Gaps = 4/309 (1%) Query: 1 MISPDSELYRKHPDWCLHVPERRRTEARWQLVLDYTRQEVRDFIYDSLSAIFSSVPVAYV 60 M+SPDS+LYR+HP+WC+HVP R R+E+R QLVLD +R++V+D+I +S I S ++YV Sbjct: 313 MVSPDSDLYREHPNWCIHVPNRPRSESRNQLVLDLSRKDVQDYIIKVVSDILESANISYV 372 Query: 61 KWDMNRCLTEIGSADLPPERQPETAHRYVLGLYELLERITSAFPHILFESCSSGGGRFDP 120 KWDMNR +TEIGSA LPPERQ ETAHRY+LGLY +LE IT+ F LFESC+ GGGRFDP Sbjct: 373 KWDMNRNMTEIGSALLPPERQRETAHRYILGLYRILEEITTRFLMFLFESCAGGGGRFDP 432 Query: 121 GMLYYMPQTWTSDNTDAVERLKIQYGTSLVYPVSAIGAHVSAVPNHQVGRITPLDFRGDV 180 G LYYMPQTWTSDNTDAVERLKIQYGTS+VYP+ ++G+HVSAVPNHQV RITPL R DV Sbjct: 433 GRLYYMPQTWTSDNTDAVERLKIQYGTSIVYPLISMGSHVSAVPNHQVHRITPLKTRLDV 492 Query: 181 AMSGNFGYELDLTKFTEEDKETAKRQIITYKEIRGLVQRGDLYRLKSPFEGNEPAWMFVS 240 A+SGNFG+ELDLTK +EE+K+ AK+ + YKEIR L+Q GD YRL SPFEGNE AWMF++ Sbjct: 493 AISGNFGFELDLTKLSEEEKDLAKKYVKKYKEIRKLIQFGDFYRLLSPFEGNETAWMFIN 552 Query: 241 EDKEEALVYHFQVMAVPNAPQRFLRLAGLHPELEYEIHSEYSGEQFICGGDRLMQLGLPL 300 E+K E + ++F+V+A PN + + L L+P+ +Y + +GE + GGD LM G+ + Sbjct: 553 EEKTEFVAFYFKVLATPNDTIKRIYLKALNPDYKYALQD--TGE--VYGGDELMYAGIAI 608 Query: 301 VYEQKDYES 309 + D++S Sbjct: 609 PQLEGDFQS 617 >ref|NP_561428.1| alpha-galactosidase [Clostridium perfringens] dbj|BAB80218.1| alpha-galactosidase [Clostridium perfringens str. 13] Length = 734 Score = 374 bits (960), Expect = e-102, Method: Compositional matrix adjust. Identities = 187/315 (59%), Positives = 233/315 (73%), Gaps = 4/315 (1%) Query: 1 MISPDSELYRKHPDWCLHVPERRRTEARWQLVLDYTRQEVRDFIYDSLSAIFSSVPVAYV 60 MISP S+LY KHP+WC+H+P R R++AR QL+LD +R+EV D+I +S+S I S ++YV Sbjct: 419 MISPISKLYEKHPNWCIHIPGRTRSQARSQLILDLSRKEVCDYIIESVSKILESANISYV 478 Query: 61 KWDMNRCLTEIGSADLPPERQPETAHRYVLGLYELLERITSAFPHILFESCSSGGGRFDP 120 KWDMNR +TE+GS L ERQ ETAHRY+LGLY ++E ITS FP++LFESCS GGGRFDP Sbjct: 479 KWDMNRNMTEVGSLGLTSERQRETAHRYILGLYRVMEEITSRFPNVLFESCSGGGGRFDP 538 Query: 121 GMLYYMPQTWTSDNTDAVERLKIQYGTSLVYPVSAIGAHVSAVPNHQVGRITPLDFRGDV 180 G+LYYMPQTWTSD+TDA+ERLKIQ+GTS+VYP ++G HVSAVPNHQ RITPL+ RG Sbjct: 539 GILYYMPQTWTSDDTDAIERLKIQFGTSMVYPQISMGCHVSAVPNHQANRITPLETRGVS 598 Query: 181 AMSGNFGYELDLTKFTEEDKETAKRQIITYKEIRGLVQRGDLYRLKSPFEGNEPAWMFVS 240 AM+GNFGYELD+TK +EE+KE K QI YKEIR VQ G LYRLKSPF NE AWM +S Sbjct: 599 AMAGNFGYELDITKLSEEEKEELKEQISLYKEIRETVQFGTLYRLKSPFNSNEVAWMMIS 658 Query: 241 EDKEEALVYHFQVMAVPNAPQRFLRLAGLHPELEYEIHSEYSGEQFICGGDRLMQLGLPL 300 EDK E +V + + A+ N L+L L + EYEI GE + GD LM +GL + Sbjct: 659 EDKNEIVVSYVRQWALVNESFSNLKLTALDKDSEYEI----IGEDIVLSGDELMYIGLNI 714 Query: 301 VYEQKDYESGWFRVR 315 DY S +R++ Sbjct: 715 PELYGDYVSKLWRLK 729 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.321 0.138 0.426 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 517,253,480 Number of extensions: 22002206 Number of successful extensions: 42313 Number of sequences better than 1.0e-05: 53 Number of HSP's gapped: 42252 Number of HSP's successfully gapped: 53 Length of query: 322 Length of database: 757,237,578 Length adjustment: 126 Effective length of query: 196 Effective length of database: 415,135,104 Effective search space: 81366480384 Effective search space used: 81366480384 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 126 (53.1 bits)