BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0596.0021.p (1658 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_00061713.1| hypothetical protein [Clostridium thermocellu... 139 6e-31 ref|ZP_00061491.1| hypothetical protein [Clostridium thermocellu... 137 2e-30 ref|ZP_00181073.1| hypothetical protein Mther097701 [Moorella th... 130 4e-28 ref|ZP_00181569.1| hypothetical protein Mther032101 [Moorella th... 124 2e-26 ref|NP_485816.1| hypothetical protein [Nostoc sp. PCC 7120] >gi|... 108 2e-21 >ref|ZP_00061713.1| hypothetical protein [Clostridium thermocellum ATCC 27405] Length = 710 Score = 139 bits (350), Expect = 6e-31, Method: Compositional matrix adjust. Identities = 68/173 (39%), Positives = 98/173 (56%), Gaps = 1/173 (0%) Query: 1481 FKDIDKHWAKENILAAVERNIVKGYEDGTFRPDGEVTRSEFAVILGRALKAN-LEQAGLS 1539 F DI HWA+E I RN+V GY DG+ +PD E+TR+E AVI+ ++ +E L Sbjct: 521 FNDIAGHWAEEFIAKLAARNVVSGYPDGSVKPDIEITRAEMAVIVVKSAGLEPVENVSLK 580 Query: 1540 FKDEGSIPSWAKPYIANAVQSGWITGYTDQTFRPDGKITRLEMIAMIVRALDWKPDPKAK 1599 FKD IP+WA Y+ V++G I GY D TFRP +TR EM+ +I++A ++ + Sbjct: 581 FKDADQIPAWAAGYVQAGVEAGIIAGYEDNTFRPSRNLTREEMVVLIMKAYEFGAVENPE 640 Query: 1600 APFTDAERIPAWGQPYAAAAYDAGLIEGKDNNRLAPNDQATRAEAITMVLRLI 1652 F DA I W +P+ A + G + G +N P TRAEA T++ + I Sbjct: 641 FSFIDASEIGDWSKPFVGKAVELGFVVGYPDNTFKPKKSVTRAEAFTVLWKAI 693 Score = 90.9 bits (224), Expect = 2e-16, Method: Compositional matrix adjust. Identities = 45/114 (39%), Positives = 66/114 (57%), Gaps = 3/114 (2%) Query: 1480 KFKDIDK--HWAKENILAAVERNIVKGYEDGTFRPDGEVTRSEFAVILGRALK-ANLEQA 1536 KFKD D+ WA + A VE I+ GYED TFRP +TR E V++ +A + +E Sbjct: 580 KFKDADQIPAWAAGYVQAGVEAGIIAGYEDNTFRPSRNLTREEMVVLIMKAYEFGAVENP 639 Query: 1537 GLSFKDEGSIPSWAKPYIANAVQSGWITGYTDQTFRPDGKITRLEMIAMIVRAL 1590 SF D I W+KP++ AV+ G++ GY D TF+P +TR E ++ +A+ Sbjct: 640 EFSFIDASEIGDWSKPFVGKAVELGFVVGYPDNTFKPKKSVTRAEAFTVLWKAI 693 >ref|ZP_00061491.1| hypothetical protein [Clostridium thermocellum ATCC 27405] Length = 4596 Score = 137 bits (346), Expect = 2e-30, Method: Compositional matrix adjust. Identities = 62/174 (35%), Positives = 100/174 (57%), Gaps = 1/174 (0%) Query: 1481 FKDIDKHWAKENILAAVERNIVKGYEDGTFRPDGEVTRSEFAVILGRALKANLEQA-GLS 1539 F DI HW E + R IV GY D T RP+ +TR+E V++ +A + + L+ Sbjct: 4419 FTDIKGHWTAEFFADLLRREIVNGYPDRTLRPNAPITRAEATVMVVKAAGFEVSDSIPLT 4478 Query: 1540 FKDEGSIPSWAKPYIANAVQSGWITGYTDQTFRPDGKITRLEMIAMIVRALDWKPDPKAK 1599 D+ S+P+WAKPY+A A+ G + GY D +FRP ++TR E + +++RA + Sbjct: 4479 CTDKDSVPAWAKPYVATAMHKGVVKGYEDGSFRPSNRLTRQETVVLVLRAFGIEEAQDKT 4538 Query: 1600 APFTDAERIPAWGQPYAAAAYDAGLIEGKDNNRLAPNDQATRAEAITMVLRLIK 1653 F D+++IPAW + Y A + G+I+G +N P + TRAE +T++ + I+ Sbjct: 4539 LAFADSDKIPAWSRGYIKKAVELGIIKGYSDNTFGPQREITRAEVVTIISKCIQ 4592 Score = 97.8 bits (242), Expect = 2e-18, Method: Compositional matrix adjust. Identities = 48/115 (41%), Positives = 70/115 (60%), Gaps = 5/115 (4%) Query: 1482 KDIDKHWAKENILAAVERNIVKGYEDGTFRPDGEVTRSEFAVILGRALKANLEQA---GL 1538 KD WAK + A+ + +VKGYEDG+FRP +TR E V++ RA +E+A L Sbjct: 4482 KDSVPAWAKPYVATAMHKGVVKGYEDGSFRPSNRLTRQETVVLVLRAF--GIEEAQDKTL 4539 Query: 1539 SFKDEGSIPSWAKPYIANAVQSGWITGYTDQTFRPDGKITRLEMIAMIVRALDWK 1593 +F D IP+W++ YI AV+ G I GY+D TF P +ITR E++ +I + + K Sbjct: 4540 AFADSDKIPAWSRGYIKKAVELGIIKGYSDNTFGPQREITRAEVVTIISKCIQLK 4594 Score = 80.5 bits (197), Expect = 3e-13, Method: Compositional matrix adjust. Identities = 35/110 (31%), Positives = 59/110 (53%) Query: 1549 WAKPYIANAVQSGWITGYTDQTFRPDGKITRLEMIAMIVRALDWKPDPKAKAPFTDAERI 1608 W + A+ ++ + GY D+T RP+ ITR E M+V+A ++ TD + + Sbjct: 4426 WTAEFFADLLRREIVNGYPDRTLRPNAPITRAEATVMVVKAAGFEVSDSIPLTCTDKDSV 4485 Query: 1609 PAWGQPYAAAAYDAGLIEGKDNNRLAPNDQATRAEAITMVLRLIKKVEQQ 1658 PAW +PY A A G+++G ++ P+++ TR E + +VLR E Q Sbjct: 4486 PAWAKPYVATAMHKGVVKGYEDGSFRPSNRLTRQETVVLVLRAFGIEEAQ 4535 >ref|ZP_00181073.1| hypothetical protein Mther097701 [Moorella thermoacetica ATCC 39073] Length = 595 Score = 130 bits (326), Expect = 4e-28, Method: Compositional matrix adjust. Identities = 74/180 (41%), Positives = 98/180 (54%), Gaps = 7/180 (3%) Query: 1481 FKDIDKHWAKENILAAVERNIVKGYEDGTFRPDGEVTRSEFAVILGRALKAN-LEQAGLS 1539 KD+ HWA + + VE +V GY DGTFRPD EVTR+E A IL RALK L Sbjct: 411 LKDMQGHWAADAVDRLVETGVVSGYPDGTFRPDLEVTRAEIAAILVRALKLTPTNNQELK 470 Query: 1540 FKDEGSIPSWAKPYIANAVQSGWITGYTD----QTFRPDGKITRLEMIAMIVRALDWKPD 1595 FKD+ +IP+WAK ++ AV+ G + GY TF D +TR EM ++ R L K Sbjct: 471 FKDDATIPTWAKDAVSIAVKEGLVKGYLQPDGTMTFEADRPVTRAEMAVLVARVLRKKLG 530 Query: 1596 --PKAKAPFTDAERIPAWGQPYAAAAYDAGLIEGKDNNRLAPNDQATRAEAITMVLRLIK 1653 + FTDA IPAW + A G++ G +N + TRAEA M+LRL++ Sbjct: 531 EVTPMELKFTDAVMIPAWAKSDVGVAVAEGIVVGYPDNTFRAENHVTRAEAAVMILRLLR 590 Score = 69.7 bits (169), Expect = 5e-10, Method: Compositional matrix adjust. Identities = 39/110 (35%), Positives = 59/110 (53%), Gaps = 4/110 (3%) Query: 1549 WAKPYIANAVQSGWITGYTDQTFRPDGKITRLEMIAMIVRALDWKPDPKAKAPFTDAERI 1608 WA + V++G ++GY D TFRPD ++TR E+ A++VRAL P + F D I Sbjct: 418 WAADAVDRLVETGVVSGYPDGTFRPDLEVTRAEIAAILVRALKLTPTNNQELKFKDDATI 477 Query: 1609 PAWGQPYAAAAYDAGLIEG---KDNNRLAPNDQ-ATRAEAITMVLRLIKK 1654 P W + + A GL++G D D+ TRAE +V R+++K Sbjct: 478 PTWAKDAVSIAVKEGLVKGYLQPDGTMTFEADRPVTRAEMAVLVARVLRK 527 >ref|ZP_00181569.1| hypothetical protein Mther032101 [Moorella thermoacetica ATCC 39073] Length = 267 Score = 124 bits (311), Expect = 2e-26, Method: Compositional matrix adjust. Identities = 75/186 (40%), Positives = 101/186 (54%), Gaps = 9/186 (4%) Query: 1481 FKDIDKHWAKENILAAVERNIVKGYEDGTFRPDGEVTRSEFAVILGRALK-ANLEQAGLS 1539 FKD+ HWA E + +I+ GY DGTF+PD ++TR+E IL RALK A + L Sbjct: 80 FKDMQGHWAAETVSRLASMDIISGYPDGTFKPDNQITRAEATAILARALKLAPGSEQDLK 139 Query: 1540 FKDEGSIPSWAKPYIANAVQSGWITGYTDQ----TFRPDGKITRLEMIAMIVRALDWK-- 1593 FKD +IP WA+ +A A + G I GY TF P I+R E+ A++VR L K Sbjct: 140 FKDNAAIPEWARGVVAAAAREGLIKGYPQPDGSVTFVPGRSISRAELAAIVVRILAMKSG 199 Query: 1594 -PDPKAKAPFTDAERIPAWGQPYAAAAYDAGLIEGKDNNRLAPNDQATRAEAITMVLRLI 1652 P P A+ F D IP W + A A +G++ G +N TRAEA M+LRL+ Sbjct: 200 APAP-AELKFADTASIPDWARQSVAQAVASGIVAGYPDNTFQAERPVTRAEAAAMILRLL 258 Query: 1653 KKVEQQ 1658 K+ Q Sbjct: 259 DKIGTQ 264 Score = 58.2 bits (139), Expect = 2e-06, Method: Compositional matrix adjust. Identities = 40/119 (33%), Positives = 58/119 (48%), Gaps = 6/119 (5%) Query: 1538 LSFKDEGSIPSWAKPYIANAVQSGWITGYTDQTFRPDGKITRLEMIAMIVRALDWKPDPK 1597 ++FKD WA ++ I+GY D TF+PD +ITR E A++ RAL P + Sbjct: 78 VTFKDMQG--HWAAETVSRLASMDIISGYPDGTFKPDNQITRAEATAILARALKLAPGSE 135 Query: 1598 AKAPFTDAERIPAWGQPYAAAAYDAGLIEG----KDNNRLAPNDQATRAEAITMVLRLI 1652 F D IP W + AAA GLI+G + P +RAE +V+R++ Sbjct: 136 QDLKFKDNAAIPEWARGVVAAAAREGLIKGYPQPDGSVTFVPGRSISRAELAAIVVRIL 194 >ref|NP_485816.1| hypothetical protein [Nostoc sp. PCC 7120] pir||AB2028 hypothetical protein all1776 [imported] - Nostoc sp. (strain PCC 7120) dbj|BAB73475.1| ORF_ID:all1776~hypothetical protein [Nostoc sp. PCC 7120] Length = 669 Score = 108 bits (269), Expect = 2e-21, Method: Compositional matrix adjust. Identities = 66/187 (35%), Positives = 104/187 (55%), Gaps = 12/187 (6%) Query: 1480 KFKDIDKHWAKENILAAVERNIVKGYEDGTFRPDGEVTRSEFAVILGRALKANLEQAGLS 1539 +F DI HWA+ I A R I+ GY DGTFRP+ VTR+EFA I+ ++++ ++ Sbjct: 7 RFSDIPNHWARPFIEALAARRILNGYPDGTFRPNNSVTRAEFAAIVAAVFNISVKRPYIN 66 Query: 1540 FKDEGSIPS--WAKPYIANAVQSGWITGYTDQTFRPDGKITRLEMIAMIVRALD----WK 1593 F D +P+ WA I A ++G+++G+ D+TFRP +I+R +++ +V L+ K Sbjct: 67 FTD---VPTSNWAANAIKKAYETGFLSGFPDKTFRPSNQISRGDILVSLVNGLEIATKIK 123 Query: 1594 PDPKAKAP--FTDAERIPAWGQPYAAAAYDAGLIEGKDNNR-LAPNDQATRAEAITMVLR 1650 PD K P + D IPA+G+ A A AGLI N + L ATR + T++ + Sbjct: 124 PDLLEKLPEIYQDGANIPAYGRNQIAIATSAGLISSFPNIKSLNFTTAATRGDVATIIYQ 183 Query: 1651 LIKKVEQ 1657 + + Q Sbjct: 184 ALVYLGQ 190 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.314 0.131 0.380 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 2,337,387,102 Number of extensions: 100128781 Number of successful extensions: 183994 Number of sequences better than 1.0e-05: 144 Number of HSP's gapped: 183429 Number of HSP's successfully gapped: 191 Length of query: 1658 Length of database: 757,237,578 Length adjustment: 139 Effective length of query: 1519 Effective length of database: 379,838,817 Effective search space: 576975163023 Effective search space used: 576975163023 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 133 (55.8 bits)