BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0596.0013.p (480 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_00035232.1| COG1621: Beta-fructosidases (levanase/inverta... 218 3e-55 ref|ZP_00037781.1| COG1621: Beta-fructosidases (levanase/inverta... 215 2e-54 ref|NP_810673.1| glycosylhydrolase [Bacteroides thetaiotaomicron... 169 2e-40 gb|EAA69589.1| hypothetical protein FG02067.1 [Gibberella zeae P... 118 4e-25 gb|EAC73616.1| unknown [environmental sequence] 112 2e-23 >ref|ZP_00035232.1| COG1621: Beta-fructosidases (levanase/invertase) [Enterococcus faecium] Length = 447 Score = 218 bits (555), Expect = 3e-55, Method: Compositional matrix adjust. Identities = 144/469 (30%), Positives = 230/469 (49%), Gaps = 59/469 (12%) Query: 17 IPYYEDGEFKLFYLHGWRDNYREGLDHGWHLLGTKDFVNYREDGACKIEGG--------- 67 +PY ++G+F +++ R+ G GW L TKDFV++ + G + G Sbjct: 1 MPYGKEGKFFIYHQRDTRNPVPVGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIY 60 Query: 68 TGHILKVDDVYHMFYC-----IFPEGK--QFACHAVSKDLRTWEPIPEDTFGPDGEIYEL 120 G + + + H FY +GK Q HA S D WE E Y++ Sbjct: 61 AGSVFEANGKVHAFYTGYNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDI 120 Query: 121 ADWRDPFVFWNEEEGQYWMLIAALAKGPTNRKGCTGLL---SSKDLKHWEYREPLYAPNL 177 +WRDPFV W++E+ +Y +++ A + ++ TG L +SKDL +WE++ +AP L Sbjct: 121 RNWRDPFVVWDDEKEEYLLILGA--RKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGL 178 Query: 178 HVGAHECPDLFRMGDWWYLIYSSYTGRFGTFYRMSRSLNGPWITPPEEAFDGRAYYAAKS 237 + E P+LF++GDWWYL+YS Y+ YRMS+SL GPWI P +EAFDGRAYYA ++ Sbjct: 179 YT-MFEMPELFKIGDWWYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRT 237 Query: 238 VSDGQKRYLFGWNPTKEDDLFGWNPPKAPGKDYDTWDWGGNLVVHEIVQRPDGTLGVKAP 297 DG++R LFGW PTK+ + D + + WGG LV HE+ Q+ D +LGVK Sbjct: 238 AYDGKRRVLFGWVPTKDQE-----------NDKNNYLWGGTLVPHEVYQKEDFSLGVKPI 286 Query: 298 ETVDSAFARQVPAHFYGVAGEWSITDEVIRCESPYSFAGCITQEELPDQCKISTTVRFSG 357 + V +AF + + +A + E+ + + + TT+ F Sbjct: 287 DEVWNAF-----INLHDIAD--------VEIETADTREEVTLVKNSGTLFNLETTISFDE 333 Query: 358 STQGLGFMLRAGEGLDFAYYITLEPERSRIAFRGPIMQSEEGGKTFPY----DMELERPL 413 + L D +Y + R+ F + +P+ D L RP+ Sbjct: 334 NLSDFSLRLYKNLETDESYEYRFNLDEGRLTF--------DKNPCYPWFQYMDKGLIRPI 385 Query: 414 KLLPDQEYELKVFIDGTICEIYVGGDVAMSARMYDIQQGKLALFVSQGA 462 L P+Q Y+LKV +D I +Y+ G +A++AR++ + + + G+ Sbjct: 386 SLKPNQSYKLKVIVDDNIMTLYIDG-IALNARVHGCFGQAITITATNGS 433 >ref|ZP_00037781.1| COG1621: Beta-fructosidases (levanase/invertase) [Enterococcus faecium] Length = 466 Score = 215 bits (547), Expect = 2e-54, Method: Compositional matrix adjust. Identities = 156/499 (31%), Positives = 239/499 (47%), Gaps = 69/499 (13%) Query: 1 MKEFFYRPENAWVGDVIPYYEDGEFKLFYLHGWRDNYREGLDHGWHLLGTKDFVNYREDG 60 M++ FY+P++ W GD +P+Y+DG F LF+ R+ GW L+ T DFV + E G Sbjct: 1 MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFGWSLVTTTDFVKFEEYG 60 Query: 61 ACKIEGGT---------GHILKVDDVYHMFYC-------IFPEGKQFACHAVSKDLRTWE 104 GG G I++ + Y Y + + + A SKDL W+ Sbjct: 61 EVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDGKTSEVLMIASSKDLIHWK 120 Query: 105 PIPEDTFGPDGEIYELADWRDPFVFWNEEEGQYWMLIAALAKGPTNRKGCTGLL---SSK 161 E E Y+ DWRDPF ++++ G+Y +++ A + P ++K TG L S+ Sbjct: 121 KDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGA--RKPASKKKPTGRLVYFESE 178 Query: 162 DLKHWEYREPLYAPNLHVGAHECPDLFRMGDWWYLIYSSYTGRFGTFYRMSRSLNGPWIT 221 DLK W ++ +A N E PD+F + WYL++S Y T YR+ L W + Sbjct: 179 DLKDWNFKGDYWANN-EFNMIEMPDIFEIDGTWYLLFSEYCEDKKTKYRVGAGLFSDWKS 237 Query: 222 PPEEAFDGRAYYAAKSVSDGQK-RYLFGWNPTKEDDLFGWNPPKAPGKDYDTWDWGGNLV 280 P +EAFDG+AYYAA++V + R+L GW TKE + D + WDWGG LV Sbjct: 238 PVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGE-----------NDQNNWDWGGALV 286 Query: 281 VHEIVQRPDGTLGVKAPETVDSAFARQVPAHFYGVAGEWSITDEVIRCESPYSFAGCITQ 340 H+I+Q+ D TLGVK PE + F P + + E+ + VI Sbjct: 287 PHQIIQKTDKTLGVKLPEKIIENFKEITPFSSFKLEKEFGKKERVI-------------V 333 Query: 341 EELPDQCKISTTVRFSGSTQGLGFMLRAGEGLDFAYYITLEPERSRIAFRGPIMQSEEGG 400 ++ D TT+ + T+ L Y + +++ F G Sbjct: 334 PKIEDYFLFETTLTITSLTKEFALKLFVDSDTQEGYEFRISLINNQLTF----------G 383 Query: 401 KT--FP----YDMELERPLKLLPDQEYELKVFIDGTICEIYVGGDVAMSARMYDIQQGKL 454 KT +P +D L+RPL L +EYELK+ +D TI +Y+ +VA+S RMYD L Sbjct: 384 KTPNYPWPQMFDKGLQRPLFLEIGKEYELKLIVDDTIAVLYI-DNVALSTRMYDKVGTAL 442 Query: 455 ALFVSQGAAEFHGVKIEAL 473 ++ V++GA +K++ L Sbjct: 443 SMTVTEGA-----IKVDKL 456 >ref|NP_810673.1| glycosylhydrolase [Bacteroides thetaiotaomicron VPI-5482] gb|AAO76867.1| glycosylhydrolase [Bacteroides thetaiotaomicron VPI-5482] Length = 523 Score = 169 bits (428), Expect = 2e-40, Method: Compositional matrix adjust. Identities = 140/507 (27%), Positives = 210/507 (41%), Gaps = 71/507 (14%) Query: 3 EFFYRPENAWVGDVIPYYE--DGEFKLFYLHGWRDNYREGLDHGWHLLGTKDFVNYREDG 60 +Y+P+ +VGD +P+Y+ +FK+ YL +R N E H + TKD Y G Sbjct: 50 SMYYKPQVGFVGDPMPFYDPVAKDFKVMYLQDYRPN-PEATYHPIFGVATKDGATYESLG 108 Query: 61 ACKIEGG---------TGHIL--KVDDVYHMFYC------IFPEGKQFACHAVSKDLRTW 103 GG TG + D +Y+ FY + Q A S D +TW Sbjct: 109 ELISCGGRDEQDAAIGTGGTIYNPADKLYYTFYTGNKFKPSSDQNAQVVMVATSPDFKTW 168 Query: 104 EP-----IPEDTFGPDGEIYELADWRDPFVFWNEEEGQYWMLIAALAKGPTNRKGCTGLL 158 + DT+G Y+ D+RDPF+F E+G Y MLIA G KG Sbjct: 169 TKNRTFYLKGDTYG-----YDKNDFRDPFLF-QTEDGVYHMLIATRKNG----KGHIAEF 218 Query: 159 SSKDLKHWEYREPLYAPNLHVGAHECPDLFRMGDWWYLIYSSYTGRFGTF-YRMSRSLN- 216 +S DLK WE + + +ECPD+F+MGDWWYLIYS Y R+L Sbjct: 219 TSADLKEWE-SAGTFMTMMWDRFYECPDVFKMGDWWYLIYSEQASFMRKVQYFKGRTLED 277 Query: 217 ---------GPWITPPEEAFDGRAYYAAKSVSDGQKRYLFGWNPTKEDDLFGWNPPKAPG 267 G W E D RA+YA K+ SDG RY++GW PT+ + G P Sbjct: 278 LKATTANDAGIWPDNREGMLDSRAFYAGKTASDGTNRYIWGWCPTRAGNDNGNVGDVEP- 336 Query: 268 KDYDTWDWGGNLVVHEIVQRPDGTLGVKAPETVDSAFARQVPAHFYGVAGEWSITDEVIR 327 +W GNLV ++Q DGTL + P+ +D + G +I Sbjct: 337 ------EWAGNLVAQRLIQHEDGTLTLGVPDAIDRKYTSAQEVKVMAKDG------NMIE 384 Query: 328 CESPYSF--AGCITQEELPDQCKISTTVRFSGSTQGLGFMLRAGEGLDFAYYITLEPERS 385 Y+ + L KIS TV+ + +T G G Y I + + Sbjct: 385 SGKTYTLGEGASVIFNRLKVHNKISFTVKTASNTDRFGISFVRGTDSASWYSIHVNADEG 444 Query: 386 RIAFRGPIMQSEEGGKTFPYDMELERPLKLLPDQEYELKVFIDGTICEIYVGGDVAMSAR 445 + F E+ G Y + + D EY + ++ D ++C Y+ ++ + R Sbjct: 445 KANF-------EKDGDDAKY--LFDNKFNIPADNEYRVTIYSDQSVCVTYINDQLSFTNR 495 Query: 446 MYDIQQGKLALFVSQGAAEFHGVKIEA 472 +Y +Q+ +L +G V++ Sbjct: 496 IYQMQKNPWSLCCYKGEITVSDVQVST 522 >gb|EAA69589.1| hypothetical protein FG02067.1 [Gibberella zeae PH-1] Length = 467 Score = 118 bits (295), Expect = 4e-25, Method: Compositional matrix adjust. Identities = 97/306 (31%), Positives = 138/306 (45%), Gaps = 52/306 (16%) Query: 15 DVIPYYEDGEFKLFYLHG--WRDNYREGLDHGWHLLGTKDFVNYREDGACKIEGGTGH-- 70 DVIP D + +F+L ++ + L W L + + + + D I + Sbjct: 34 DVIPLLVDDTYHVFHLSTPPSTKHHPQRLRTTWSRLRSTNLLEWERDPQPVISPAESNAD 93 Query: 71 -----------ILKVDDVYHMFYC----------IFPEGKQFACHAVSKDLRTWEPIPED 109 +L +D ++FY K F H D T +PI Sbjct: 94 PDADGVWTGCAVLDLDQNMNIFYTGYNLSRNGQQTILRTKAFDRHGTRFD-STSQPI--T 150 Query: 110 TFGPDGEIYELADWRDPFVFWNEEEGQYWMLIAA-LAKGPTNRKGCTGLLSSKDLKHWEY 168 G + +E D+RDPFVF+N EG+YWML+ LA GP +GC LL+S DL+ W Sbjct: 151 ILGNGLDKFERIDFRDPFVFFNRAEGRYWMLVGTRLANGPYWSRGCIALLTSPDLEAWTV 210 Query: 169 R-EPLYAPNLHVGAHECPDLFRMGD-WWYLIYSSYTG-RFGTFYRMSRSLNGPWITPPEE 225 EPLY PN V ECP+LF + + WYL+YS + GT YRM+ + GP+ P + Sbjct: 211 SPEPLYTPN-DVFCPECPELFTLPNGKWYLVYSRFHAPNAGTVYRMADTPYGPFRVPRDG 269 Query: 226 A---FDGRAYYAAKS---VSDGQKRYLFGWNPTKEDDLFGWNPPKAPGKDYDTWDWGGNL 279 + DGR +YAAKS D KR FGW D D + W WGG+L Sbjct: 270 SHGRLDGRRWYAAKSCPKARDPDKRICFGWIGDYAD-------------DENKWLWGGDL 316 Query: 280 VVHEIV 285 + ++ Sbjct: 317 AITRVM 322 >gb|EAC73616.1| unknown [environmental sequence] Length = 258 Score = 112 bits (280), Expect = 2e-23, Method: Compositional matrix adjust. Identities = 77/263 (29%), Positives = 124/263 (47%), Gaps = 29/263 (11%) Query: 225 EAFDGRAYYAAKSVSDGQKRYLFGWNPTKEDDLFGWNPPKAPGKDYDTWDWGGNL-VVHE 283 + DGR +YA KS +DG++R F W P + D L + W WGG++ HE Sbjct: 5 DTLDGRRFYAGKSCTDGKRRMAFAWIPYRLDHLAN-----------ERWVWGGDIGSPHE 53 Query: 284 IVQRPDGTLGVKAPETVDSAFARQVPAHFYGVAGEWSIT-DEVIRCESPYSFAGC-ITQE 341 + DGTL VK PE V +++ +WS + I E+ +++ C +T Sbjct: 54 YISLNDGTLAVKLPEEVAKSYSNSNKFEIKNSFNDWSFDGNGSINVEAISTYSSCFLTGP 113 Query: 342 ELPDQCKISTTVRFSGSTQGLGFMLRAGEGLDFAYYITLEPERSRIAF-RGPI------- 393 + D T + S T G +L + L +YY+++EP +SR+ R P+ Sbjct: 114 KRDDDLLFETNLTISEGTTAAGILLESSSDLFRSYYLSIEPLKSRVMLNRWPLVSDPTWT 173 Query: 394 -MQSEEGGKTFPYDMELERPL--KLLP----DQEYELKVFIDGTICEIYVGGDVAMSARM 446 M E K F +E + PL ++LP + Y +K+ GT E +V V S R+ Sbjct: 174 WMVLENRHKLFDNVVEQDYPLVDRILPELPKNNTYNIKITRKGTNVECWVCDQVVASYRI 233 Query: 447 YDIQQGKLALFVSQGAAEFHGVK 469 Y+ Q+ LFV +G+A F+ ++ Sbjct: 234 YEEQENMFGLFVREGSASFNNLE 256 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.321 0.141 0.467 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 837,331,037 Number of extensions: 39102414 Number of successful extensions: 64179 Number of sequences better than 1.0e-05: 9 Number of HSP's gapped: 64178 Number of HSP's successfully gapped: 9 Length of query: 480 Length of database: 757,237,578 Length adjustment: 129 Effective length of query: 351 Effective length of database: 406,989,807 Effective search space: 142853422257 Effective search space used: 142853422257 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 128 (53.9 bits)