BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0596.0010.p (528 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gb|AAF44125.1| exo-inulinase [Pseudomonas mucidolens] 593 e-168 gb|AAL82575.1| exo-inulinase [Paenibacillus polymyxa] 509 e-143 ref|NP_390581.1| levanase [Bacillus subtilis] >gi|134175|sp|P056... 480 e-134 prf||1404371A levanase 480 e-134 pir||A27286 levanase (EC 3.2.1.65) precursor - Bacillus subtilis... 480 e-134 >gb|AAF44125.1| exo-inulinase [Pseudomonas mucidolens] Length = 501 Score = 593 bits (1528), Expect = e-168, Method: Compositional matrix adjust. Identities = 274/474 (57%), Positives = 341/474 (71%), Gaps = 1/474 (0%) Query: 28 YSKESYRPQFHYSPPAMWLNDPNGMVYFEGEYHLMYQHHPESTVWGPMHWGHAVSKDLVN 87 Y +E +RPQFH++P W+NDPNG+VY+EGEYHL YQ+HP WGPMHWGHAVSKDL++ Sbjct: 5 YYQEKFRPQFHFTPETNWMNDPNGLVYYEGEYHLFYQYHPFGNTWGPMHWGHAVSKDLIH 64 Query: 88 WETLPVALNPDHNGAIFSGSAVVDWKDTSGFFDGGSGLVAIFTHADIYPDSDRPRQRQSL 147 W+ LP+AL PDH G IFSGSAV+DW+DTSGFF+GG+G+VA+FTHAD +PDS RPRQRQSL Sbjct: 65 WDHLPIALYPDHIGQIFSGSAVIDWQDTSGFFNGGAGMVALFTHADKFPDSGRPRQRQSL 124 Query: 148 AYSKDKGRSWSFYAGNPVLSDTGITDFRDPKVFWMEVAGVWVMVLACGDHIRFYRSANLK 207 AYS D+GR+W Y GNP +D +TDFRDPKVFW + + W+M+LA G+ +R Y S+NLK Sbjct: 125 AYSTDRGRTWIKYEGNPFFADEAMTDFRDPKVFWHKASNKWIMILAAGNCVRIYHSSNLK 184 Query: 208 DWDFASEFGKSEGSHYGVWECPDLFELSVDGS-NLKKWVLLVSIGDNPDYPEGSKTQYFI 266 W FASEFG +EGSH GVWECPDLFEL VDG N KKWV++VSIG++ +Y EGS+TQYFI Sbjct: 185 QWKFASEFGANEGSHTGVWECPDLFELPVDGDRNRKKWVMVVSIGNSDEYLEGSRTQYFI 244 Query: 267 GSFDGWKFVNENTPDTVLWLDEGRDNYAAVSWSDIPEEDGRRVIIGWMSNWSYARQIPTG 326 G FDG +F NEN P+TVLW+D GRDNYA V+WSDIPEEDGRR+ I WMSNW YA PT Sbjct: 245 GQFDGIRFTNENDPETVLWVDHGRDNYAGVTWSDIPEEDGRRLFIAWMSNWKYANHTPTE 304 Query: 327 SWRGAMTLPRVLSLTSRNGTVVLSQMPVQEIEQLRKESLSWSDVSVTREAPFIHGTNXXX 386 WR AMT+PR LSL S L Q PV E+E LR E L W D+ VT + + Sbjct: 305 IWRSAMTIPRSLSLRSTPEGTRLFQTPVTELESLRLEKLEWKDLEVTTNGNVLSNISGDI 364 Query: 387 XXXXXXXXXRSGDEVQIKLRSSGQSETIIGYDPEREWLFIDRSNSGLTDFNPSFACKHGA 446 + E K+R S ETI+GYD E++ LFIDR+ SG++DF F CKHGA Sbjct: 365 FEIIAEFELNTALEFGFKVRKSADQETIVGYDAEQQMLFIDRTQSGVSDFCEHFPCKHGA 424 Query: 447 RLAPENGSIKLRIWLDRSVVEVFANEGLVALTDQIFPDEPIDTVWIGAETGKAE 500 + P I++ I++DRS VEVF N G + +TD IFPD+ + + A+ G + Sbjct: 425 VMIPNQNRIQMHIFVDRSSVEVFGNNGELTMTDLIFPDDSSTGIEVYAKEGNVK 478 >gb|AAL82575.1| exo-inulinase [Paenibacillus polymyxa] Length = 485 Score = 509 bits (1312), Expect = e-143, Method: Compositional matrix adjust. Identities = 254/456 (55%), Positives = 306/456 (67%), Gaps = 3/456 (0%) Query: 30 KESYRPQFHYSPPAMWLNDPNGMVYFEGEYHLMYQHHPESTVWGPMHWGHAVSKDLVNWE 89 +E YRP +H+SP + W+NDPNGMVY+EG YHL YQHHP T WGPMHWGHAVS DL+NWE Sbjct: 6 QEKYRPSYHFSPKSGWMNDPNGMVYYEGRYHLFYQHHPFGTTWGPMHWGHAVSTDLMNWE 65 Query: 90 TLPVALNPDHNGAIFSGSAVVDWKDTSGFFDGGSGLVAIFTHADIYPDSDRPRQRQSLAY 149 P+AL PD G IFSGSAVVD +DTSGFF G GLVAIFTH P +++ RQ QSLAY Sbjct: 66 EQPIALEPDELGTIFSGSAVVDEQDTSGFFGGKPGLVAIFTHHASLPGTEQVRQCQSLAY 125 Query: 150 SKDKGRSWSFYAGNPVLSDTGITDFRDPKVFWMEVAGVWVMVLACGDHIRFYRSANLKDW 209 S D G++W Y GNPVL D DFRDPKVFW + WVMVLACG IR Y S NLK+W Sbjct: 126 STDSGKTWIKYTGNPVLEDKECIDFRDPKVFWHKPTEQWVMVLACGQTIRIYHSPNLKEW 185 Query: 210 DFASEFGKSEGSHYGVWECPDLFELSVDGSNLK-KWVLLVSIGDNPDYPEGSKTQYFIGS 268 FASEFG GSH VWECPDLF L +D + KWV+LVSIGD P+ EGS+TQYF G Sbjct: 186 TFASEFGHGIGSHDAVWECPDLFPLYIDEKREQVKWVMLVSIGDVPEIREGSRTQYFTGE 245 Query: 269 FDGWKFVNENTPDTVLWLDEGRDNYAAVSWSDIPEEDGRRVIIGWMSNWSYARQIPTGSW 328 FDG FV + + V WLD GRDNYA V WSDIP EDGRR+ +GWMSNW YA Q PT W Sbjct: 246 FDGTTFVADAESEKVRWLDYGRDNYAGVCWSDIPAEDGRRLFMGWMSNWRYANQTPTERW 305 Query: 329 RGAMTLPRVLSLTSRNGTVVLSQMPVQEIEQLRKESLSWSDVSVTREAPFIHGTNXXXXX 388 RGAM++PR L+L +R GTV L Q PV+E+E LR LS ++ S + Sbjct: 306 RGAMSIPRELALETRKGTVALVQRPVRELEGLRTPVLSLTEPSWEEVRNALSALQLDCYE 365 Query: 389 XXXXXXXRSGDEVQIKLRSSGQSETIIGYDPEREWLFIDRSNSGLTDFNPSFACKHGARL 448 +GD K+R S + ET++GY + +++DR SG +DF+ FA KHGA L Sbjct: 366 LVAEFAT-TGD-FGFKVRVSDEQETLVGYTSATQEVYVDRKRSGRSDFHEDFAGKHGASL 423 Query: 449 APENGSIKLRIWLDRSVVEVFANEGLVALTDQIFPD 484 + +RI++DRS +EVF + G VA+TD IFPD Sbjct: 424 TILPDRMDIRIYVDRSSIEVFFDRGQVAITDLIFPD 459 >ref|NP_390581.1| levanase [Bacillus subtilis] sp|P05656|SACC_BACSU Levanase precursor (2,6-beta-D-fructan fructanohydrolase) emb|CAA63465.1| levanase precursor [Bacillus subtilis] emb|CAB14645.1| levanase [Bacillus subtilis subsp. subtilis str. 168] Length = 677 Score = 480 bits (1236), Expect = e-134, Method: Compositional matrix adjust. Identities = 253/482 (52%), Positives = 306/482 (63%), Gaps = 15/482 (3%) Query: 23 SAGTDYSKESYRPQFHYSPPAMWLNDPNGMVYFEGEYHLMYQHHPESTVWGPMHWGHAVS 82 +A + Y E YRPQ+H++P A W+NDPNGMVY+ GEYHL YQ+HP WGPMHWGHAVS Sbjct: 24 AADSSYYDEDYRPQYHFTPEANWMNDPNGMVYYAGEYHLFYQYHPYGLQWGPMHWGHAVS 83 Query: 83 KDLVNWETLPVALNPDHNGAIFSGSAVVDWKDTSGFFDGGSG-LVAIFTHADIYPDSDRP 141 KDLV WE LPVAL PD G IFSGSAVVD +TSGF G LVAI+T DR Sbjct: 84 KDLVTWEHLPVALYPDEKGTIFSGSAVVDKNNTSGFQTGKEKPLVAIYTQ-------DRE 136 Query: 142 -RQRQSLAYSKDKGRSWSFYAGNPVLSDTGITDFRDPKVFWMEVAGVWVMVLACGDHIRF 200 Q QS+AYS DKGR+W+ YAGNPV+ + G DFRDPKVFW E WVMVLA GD I Sbjct: 137 GHQVQSIAYSNDKGRTWTKYAGNPVIPNPGKKDFRDPKVFWYEKEKKWVMVLAAGDRILI 196 Query: 201 YRSANLKDWDFASEFGKSEGSHYGVWECPDLFELSVDGS-NLKKWVLLVSIGDNPDYPEG 259 Y S NLK W +ASEFG+ +GSH GVWECPDLFEL VDG+ N KKWV+ VS+G N G Sbjct: 197 YTSKNLKQWTYASEFGQDQGSHGGVWECPDLFELPVDGNPNQKKWVMQVSVG-NGAVSGG 255 Query: 260 SKTQYFIGSFDGWKFVNENTPDTVLWLDEGRDNYAAVSWSDIPEEDGRRVIIGWMSNWSY 319 S QYF+G FDG F NEN P+ VLW D GRD YAAVSWSDIP D RR+ +GWMSNW Y Sbjct: 256 SGMQYFVGDFDGTHFKNENPPNKVLWTDYGRDFYAAVSWSDIPSTDSRRLWLGWMSNWQY 315 Query: 320 ARQIPTGSWRGAMTLPRVLSLTSRNGTVVLSQMPVQEIEQLRKESLSWSDVSVTREAPFI 379 A +PT WR A ++PR L L + V + Q PV+E+E +R S W +++++ + + Sbjct: 316 ANDVPTSPWRSATSIPRELKLKAFTEGVRVVQTPVKELETIRGTSKKWKNLTISPASHNV 375 Query: 380 ---HGTNXXXXXXXXXXXXRSGDEVQIKLRSSGQSETIIGYDPEREWLFIDRSNSGLTDF 436 + S E K+R+ T +GYD LF+DRS SG F Sbjct: 376 LAGQSGDAYEINAEFKVSPGSAAEFGFKVRTGENQFTKVGYDRRNAKLFVDRSESGNDTF 435 Query: 437 NPSFAC-KHGARLAPENGSIKLRIWLDRSVVEVFANEGLVALTDQIFPDEPIDTVWIGAE 495 NP+F K A L P NG +KLRI++DRS VEVF N+G +TD I PD + + A Sbjct: 436 NPAFNTGKETAPLKPVNGKVKLRIFVDRSSVEVFGNDGKQVITDIILPDRSSKGLELYAA 495 Query: 496 TG 497 G Sbjct: 496 NG 497 >prf||1404371A levanase Length = 677 Score = 480 bits (1235), Expect = e-134, Method: Compositional matrix adjust. Identities = 253/482 (52%), Positives = 306/482 (63%), Gaps = 15/482 (3%) Query: 23 SAGTDYSKESYRPQFHYSPPAMWLNDPNGMVYFEGEYHLMYQHHPESTVWGPMHWGHAVS 82 +A + Y E YRPQ+H++P A W+NDPNGMVY+ GEYHL YQ+HP WGPMHWGHAVS Sbjct: 24 AADSSYYDEDYRPQYHFTPEANWMNDPNGMVYYAGEYHLFYQYHPYGLQWGPMHWGHAVS 83 Query: 83 KDLVNWETLPVALNPDHNGAIFSGSAVVDWKDTSGFFDGGSG-LVAIFTHADIYPDSDRP 141 KDLV WE LPVAL PD G IFSGSAVVD +TSGF G LVAI+T DR Sbjct: 84 KDLVTWEHLPVALYPDEKGTIFSGSAVVDKNNTSGFQTGKEKPLVAIYTQ-------DRE 136 Query: 142 -RQRQSLAYSKDKGRSWSFYAGNPVLSDTGITDFRDPKVFWMEVAGVWVMVLACGDHIRF 200 Q QS+AYS DKGR+W+ YAGNPV+ + G DFRDPKVFW E WVMVLA GD I Sbjct: 137 GHQVQSIAYSNDKGRTWTKYAGNPVIPNPGKKDFRDPKVFWYEKEKKWVMVLAAGDRILI 196 Query: 201 YRSANLKDWDFASEFGKSEGSHYGVWECPDLFELSVDGS-NLKKWVLLVSIGDNPDYPEG 259 Y S NLK W +ASEFG+ +GSH GVWECPDLFEL VDG+ N KKWV+ VS+G N G Sbjct: 197 YTSKNLKQWTYASEFGQDQGSHGGVWECPDLFELPVDGNPNQKKWVMQVSVG-NGAVSGG 255 Query: 260 SKTQYFIGSFDGWKFVNENTPDTVLWLDEGRDNYAAVSWSDIPEEDGRRVIIGWMSNWSY 319 S QYF+G FDG F NEN P+ VLW D GRD YAAVSWSDIP D RR+ +GWMSNW Y Sbjct: 256 SGMQYFVGDFDGTHFKNENPPNKVLWTDYGRDFYAAVSWSDIPSTDSRRLWLGWMSNWQY 315 Query: 320 ARQIPTGSWRGAMTLPRVLSLTSRNGTVVLSQMPVQEIEQLRKESLSWSDVSVTREAPFI 379 A +PT WR A ++PR L L + V + Q PV+E+E +R S W +++++ + + Sbjct: 316 ANDVPTSPWRSATSIPRELKLKAFTEGVRVVQTPVKELETIRGTSKKWKNLTISPASHNV 375 Query: 380 ---HGTNXXXXXXXXXXXXRSGDEVQIKLRSSGQSETIIGYDPEREWLFIDRSNSGLTDF 436 + S E K+R+ T +GYD LF+DRS SG F Sbjct: 376 LAGQSGDAYEINAEFKVSPGSAAEFGFKVRTGENQFTKVGYDRRNAKLFVDRSESGNDTF 435 Query: 437 NPSFAC-KHGARLAPENGSIKLRIWLDRSVVEVFANEGLVALTDQIFPDEPIDTVWIGAE 495 NP+F K A L P NG +KLRI++DRS VEVF N+G +TD I PD + + A Sbjct: 436 NPAFNTGKETAPLKPVNGKVKLRIFVDRSSVEVFGNDGKQVITDIILPDRSSKGLELYAA 495 Query: 496 TG 497 G Sbjct: 496 NG 497 >pir||A27286 levanase (EC 3.2.1.65) precursor - Bacillus subtilis emb|CAA68542.1| unnamed protein product [Bacillus subtilis] Length = 677 Score = 480 bits (1235), Expect = e-134, Method: Compositional matrix adjust. Identities = 253/482 (52%), Positives = 306/482 (63%), Gaps = 15/482 (3%) Query: 23 SAGTDYSKESYRPQFHYSPPAMWLNDPNGMVYFEGEYHLMYQHHPESTVWGPMHWGHAVS 82 +A + Y E YRPQ+H++P A W+NDPNGMVY+ GEYHL YQ+HP WGPMHWGHAVS Sbjct: 24 AADSSYYDEDYRPQYHFTPEANWMNDPNGMVYYAGEYHLFYQYHPYGLQWGPMHWGHAVS 83 Query: 83 KDLVNWETLPVALNPDHNGAIFSGSAVVDWKDTSGFFDGGSG-LVAIFTHADIYPDSDRP 141 KDLV WE LPVAL PD G IFSGSAVVD +TSGF G LVAI+T DR Sbjct: 84 KDLVTWEHLPVALYPDEKGTIFSGSAVVDKNNTSGFQTGKEKPLVAIYTQ-------DRE 136 Query: 142 -RQRQSLAYSKDKGRSWSFYAGNPVLSDTGITDFRDPKVFWMEVAGVWVMVLACGDHIRF 200 Q QS+AYS DKGR+W+ YAGNPV+ + G DFRDPKVFW E WVMVLA GD I Sbjct: 137 GHQVQSIAYSNDKGRTWTKYAGNPVIPNPGKKDFRDPKVFWYEKEKKWVMVLAAGDRILI 196 Query: 201 YRSANLKDWDFASEFGKSEGSHYGVWECPDLFELSVDGS-NLKKWVLLVSIGDNPDYPEG 259 Y S NLK W +ASEFG+ +GSH GVWECPDLFEL VDG+ N KKWV+ VS+G N G Sbjct: 197 YTSKNLKQWTYASEFGQDQGSHGGVWECPDLFELPVDGNPNQKKWVMQVSVG-NGAVSGG 255 Query: 260 SKTQYFIGSFDGWKFVNENTPDTVLWLDEGRDNYAAVSWSDIPEEDGRRVIIGWMSNWSY 319 S QYF+G FDG F NEN P+ VLW D GRD YAAVSWSDIP D RR+ +GWMSNW Y Sbjct: 256 SGMQYFVGDFDGTHFKNENPPNKVLWTDYGRDFYAAVSWSDIPSTDSRRLWLGWMSNWQY 315 Query: 320 ARQIPTGSWRGAMTLPRVLSLTSRNGTVVLSQMPVQEIEQLRKESLSWSDVSVTREAPFI 379 A +PT WR A ++PR L L + V + Q PV+E+E +R S W +++++ + + Sbjct: 316 ANDVPTSPWRSATSIPRELKLKAFTEGVRVVQTPVKELETIRGTSKKWKNLTISPASHNV 375 Query: 380 ---HGTNXXXXXXXXXXXXRSGDEVQIKLRSSGQSETIIGYDPEREWLFIDRSNSGLTDF 436 + S E K+R+ T +GYD LF+DRS SG F Sbjct: 376 LAGQSGDAYEINAEFKVSPGSAAEFGFKVRTGENQFTKVGYDRRNAKLFVDRSESGNDTF 435 Query: 437 NPSFAC-KHGARLAPENGSIKLRIWLDRSVVEVFANEGLVALTDQIFPDEPIDTVWIGAE 495 NP+F K A L P NG +KLRI++DRS VEVF N+G +TD I PD + + A Sbjct: 436 NPAFNTGKETAPLKPVNGKVKLRIFVDRSSVEVFGNDGKQVITDIILPDRSSKGLELYAA 495 Query: 496 TG 497 G Sbjct: 496 NG 497 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.318 0.135 0.434 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 857,261,130 Number of extensions: 38178240 Number of successful extensions: 69252 Number of sequences better than 1.0e-05: 399 Number of HSP's gapped: 67973 Number of HSP's successfully gapped: 407 Length of query: 528 Length of database: 757,237,578 Length adjustment: 130 Effective length of query: 398 Effective length of database: 404,274,708 Effective search space: 160901333784 Effective search space used: 160901333784 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 128 (53.9 bits)