BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0596.0001.p (1129 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gb|AAB82454.1| beta-mannanase [Dictyoglomus thermophilum] 424 e-117 gb|AAC44232.1| beta-1,4-mannanase 376 e-102 ref|ZP_00118948.1| COG4124: Beta-mannanase [Cytophaga hutchinsonii] 326 3e-87 sp|P49425|MANA_RHOMR Mannan endo-1,4-beta-mannosidase >gi|751994... 324 8e-87 pir||JC7657 mannan endo-1,4-beta-mannosidase (EC 3.2.1.78) - Clo... 304 7e-81 >gb|AAB82454.1| beta-mannanase [Dictyoglomus thermophilum] Length = 398 Score = 424 bits (1089), Expect = e-117, Method: Compositional matrix adjust. Identities = 199/394 (50%), Positives = 271/394 (68%), Gaps = 8/394 (2%) Query: 140 YDILVGYCAPNGEKKTSMVLNGH-TSEITLPETTGFIEISAGKTWMNQGDNTIEILANWG 198 +++++GY AP G K+ S+ +NG + + P++ F + AG + G NTI I+ +WG Sbjct: 2 HELIIGYAAPYGYKENSLYVNGEFQTNVKFPQSQKFTTVYAGLIPLKNGKNTISIVKSWG 61 Query: 199 WYNIDYVKLTAAAKPEQREVVSTLMNPDATPEARALMNFMDSQYGQKIISGQQTLEDAEW 258 W+ +DY K+ A P + L+ P+ + EA+ LM+++ S YG+ +SGQ +DA W Sbjct: 62 WFLLDYFKIKKAEIPTMNPT-NKLVTPNPSKEAQKLMDYLVSIYGKYTLSGQMGYKDAFW 120 Query: 259 IGQETGKYPAILATDLMDYSPSRVENGASSTEIEKMIAWYNRGGIVSLCWHWNAPKGIGG 318 I T K+PAI D+MDYSPSRVE GASS ++E I W+N GGIV WHWNAPKG+ Sbjct: 121 IWNITDKFPAICGFDMMDYSPSRVERGASSRDVEDAIDWWNMGGIVQFQWHWNAPKGLY- 179 Query: 319 EEPGYEWWRGFYTEYTTFDVEYALDHPDSEDYHLLIRDIDEIAIQLKRLQNAGVPVLWRP 378 + PG EWWRGFYT T+FD+EYAL+HP+SEDY L+IRDID IA+QLKRLQ A VP+LWRP Sbjct: 180 DTPGKEWWRGFYTNATSFDIEYALNHPESEDYKLIIRDIDAIAVQLKRLQEAKVPILWRP 239 Query: 379 LHEAEGGWFWWGAKGPEPAKRLWKLMVDRLTSHHHLNNLIWVW-NSEKPE---WYPGDDV 434 LHEAEG WFWWGAKGPE K+LW+L+ DRL ++H +NNLIWVW ++ P+ WYPGD+ Sbjct: 240 LHEAEGRWFWWGAKGPEACKKLWRLLFDRLVNYHKINNLIWVWTTTDSPDALKWYPGDEY 299 Query: 435 VDIASVDIYNPAGDYNPSIAKYENLVSLVNGKKVIGLAENGPIPDPEMLQAYGADWSFFT 494 VDI DIY DY+PS + N+V L GKK++ L ENG IPDP++++ A W +F Sbjct: 300 VDIVGADIYLKDKDYSPSTGMFYNIVKLFGGKKLVALTENGIIPDPDLMKEQKAYWVWFM 359 Query: 495 TWTGSFIKDGATNSAEHLQRIYKHDYVVTLDELP 528 TW+G F D N H+++++ H +V+T DELP Sbjct: 360 TWSG-FENDPNKNEISHIKKVFNHPFVITKDELP 392 >gb|AAC44232.1| beta-1,4-mannanase Length = 911 Score = 376 bits (966), Expect = e-102, Method: Compositional matrix adjust. Identities = 196/460 (42%), Positives = 271/460 (58%), Gaps = 33/460 (7%) Query: 94 LEAEDGMMSGGVSVGTAGDGYSGEGYVIF--QQSGSLTLTYKTPLAGTYDILVGYCAPNG 151 +EAE S+ YSG+GY++ G+ K P G Y + Sbjct: 452 IEAETAKDGWAYSIEKENAKYSGKGYILLFGNNMGNTLYDLKIPKTGYYIFTI------- 504 Query: 152 EKKTSMVLNGHTSEI----------TLPETTGFIE--ISAGKTWMNQGDNTIEILANWGW 199 T ++N + +I +P G + + K ++ QG++T+ + + G+ Sbjct: 505 SSSTLGMVNYGSVDIWIDGELKGAAKVPNVKGKFQEVVVMKKIYLTQGEHTLSLQKSGGY 564 Query: 200 -YNIDYVKL--TAAAKPEQREVVSTLMNPDATPEARALMNFMDSQYGQKIISGQQTLEDA 256 IDY + A + V + L+ P+ P A+ LM ++ S YG+KI+SGQQ+ D Sbjct: 565 TIAIDYFTIEELVLANKNKISVDTKLVTPNPHPNAQKLMKYLASIYGEKILSGQQSSGDG 624 Query: 257 ---EWIGQETGKYPAILATDLMDYSPSRVENGASSTEIEKMIAWYNRGGIVSLCWHWNAP 313 + I T +YPA+ D MDYSPSRVE+G T++E+ I W+ GGIV+ CWHWNAP Sbjct: 625 KEIQMIFDVTKRYPAVRGFDFMDYSPSRVEHGTKGTDVEEAIKWWKSGGIVAFCWHWNAP 684 Query: 314 KGIGGEEPGYEWWRGFYTEYTTFDVEYALDHPDSEDYHLLIRDIDEIAIQLKRLQNAGVP 373 G+ ++PG EWWRGFYTE TTFD++ A+D+P+S +Y L++RDID IA QLKRLQ GVP Sbjct: 685 TGLI-DQPGKEWWRGFYTEATTFDIKKAMDNPNSTEYKLILRDIDAIAEQLKRLQKEGVP 743 Query: 374 VLWRPLHEAEGGWFWWGAKGPEPAKRLWKLMVDRLTSHHHLNNLIWVWNSEKPEWYPGDD 433 VL+RPLHEA GGWFWWGAKGPEP +LWKLM DRL ++H LNNLIWVWN + WYPGD+ Sbjct: 744 VLFRPLHEASGGWFWWGAKGPEPYIKLWKLMFDRLVNYHKLNNLIWVWNGQDAAWYPGDN 803 Query: 434 VVDIASVDIYNPAGDYNPSIAKYENLVSLVNGKKVIGLAENGPIPDPEMLQAYGADWSFF 493 VDI DIY Y+P ++ + N +K+I L E G IPDP +L+ G WS+F Sbjct: 804 YVDIIGEDIYEEKAQYSPYADRFAKALKYTNARKMIALTECGTIPDPAVLKQEGISWSWF 863 Query: 494 TTWTGSFIKDGAT-----NSAEHLQRIYKHDYVVTLDELP 528 + W G+F+ G+ N L++IY DYV+T DELP Sbjct: 864 SVWAGNFVMTGSKYNDEWNDNHMLRKIYNSDYVITKDELP 903 >ref|ZP_00118948.1| COG4124: Beta-mannanase [Cytophaga hutchinsonii] Length = 815 Score = 326 bits (835), Expect = 3e-87, Method: Compositional matrix adjust. Identities = 175/451 (38%), Positives = 259/451 (57%), Gaps = 28/451 (6%) Query: 94 LEAEDGMMSGGVSVGTAGDGYSGEGYVIFQQSGS--LTLTYKTPLAGTYDILVGYCAPNG 151 +EAE G +GG+++ TA GYSG GY+ S + LT+ P AG Y +++ Y P G Sbjct: 38 VEAESGTRAGGLTIATANAGYSGTGYITNMTSAADKLTIPVTVPSAGNYQLVIRYNGPYG 97 Query: 152 EKKTSMVLNG-HTSEITLPETTGFIEISAGKTWMNQGDNTIEILANWGWYNIDYVKLTAA 210 K + +NG S + PETTG+ +++AG +N G NTI I NWG+ + D + L Sbjct: 98 PKDQDIYVNGVFVSSLHFPETTGYTDLTAGSLTLNAGANTIGIYKNWGYTDFDKISLFTV 157 Query: 211 AKPEQREVVSTLMNPDATPEARALMNFMDSQYGQKIISGQQTLEDAEWIGQETGKYPAIL 270 + V L++ +ATPEA+AL N++ + YG +IISGQ T + + K P I Sbjct: 158 PLHDYSTVAPNLIDANATPEAKALYNYIKAGYGTRIISGQ-TSDYYNTVSPNAAKKPVIR 216 Query: 271 ATDLMDYSP--SRVENGASSTEI---------EKMIAWYN---RGGIVSLCWHWNAPKGI 316 A D Y+ S + N A+++ + I WYN + G+V+ WHW++P G Sbjct: 217 AYDFQHYTVGYSYLWNNATASHTFGWEDDGTTQAAIDWYNSTCQKGMVAFHWHWHSPSG- 275 Query: 317 GGEEPGYEWWRGFYTEYTTFDVEYALDHPDSEDYHLLIRDIDEIAIQLKRLQNAGVPVLW 376 G FYT T FDV A+ + +Y +IRDID IA QLKRLQ AGVPVLW Sbjct: 276 -----GQAGTNTFYTNSTAFDVTKAVTQ-GTPEYTAVIRDIDSIATQLKRLQAAGVPVLW 329 Query: 377 RPLHEAEGGWFWWGAKGPEPAKRLWKLMVDRLTSHHHLNNLIWVWNSEKPEWYPGDDVVD 436 RPLHEA G WFWWGAKGP A L+ ++ +R+ ++H+++NLIW W++ + WYPG+ VD Sbjct: 330 RPLHEAGGAWFWWGAKGPAAALALYDMVYNRIVNYHNIHNLIWQWSTPEANWYPGNSKVD 389 Query: 437 IASVDIYNPAGDYNPSIAKYENLVSLVNGKKVIGLAENGPIPDPEMLQAYGADWSFFTTW 496 + D Y A +Y ++ L ++VNG+K++ + ENGPIPDP++ + A W++ +W Sbjct: 390 MLGYDSYPGAYNYGTQKLIFDQLYTIVNGQKILAMTENGPIPDPDLCFSSDAKWAYVMSW 449 Query: 497 TGSFIKDGATNSAEHLQRIYKHDYVVTLDEL 527 + + NS+ L +Y H V+TLDE+ Sbjct: 450 SDLVTQQ---NSSAQLNLVYNHAKVLTLDEV 477 Score = 62.0 bits (149), Expect = 9e-08, Method: Compositional matrix adjust. Identities = 44/116 (37%), Positives = 52/116 (44%), Gaps = 2/116 (1%) Query: 538 EAEKGELTG-LEVSDEQGAYSGSGYITGMDAQGGRLRIKVDA-SPGTYSLMIRYKTGNVQ 595 EAE G G L ++ YSG+GYIT M + +L I V S G Y L+IRY Sbjct: 39 EAESGTRAGGLTIATANAGYSGTGYITNMTSAADKLTIPVTVPSAGNYQLVIRYNGPYGP 98 Query: 596 TKNSISVNGASAVEYTFADAAEWTDTVTGSYPLQKGENTIDIISAEKGTDIDYIKL 651 I VNG F + +TD GS L G NTI I TD D I L Sbjct: 99 KDQDIYVNGVFVSSLHFPETTGYTDLTAGSLTLNAGANTIGIYKNWGYTDFDKISL 154 >sp|P49425|MANA_RHOMR Mannan endo-1,4-beta-mannosidase pir||T10748 mannan endo-1,4-beta-mannosidase (EC 3.2.1.78) - Rhodothermus marinus emb|CAA62442.1| endo-1,4-beta-mannanase [Rhodothermus marinus] Length = 1021 Score = 324 bits (830), Expect = 8e-87, Method: Compositional matrix adjust. Identities = 190/455 (41%), Positives = 266/455 (58%), Gaps = 26/455 (5%) Query: 90 AGNI-LEAEDGMMSGGVSVGTAGDGYSGEGYV--IFQQSGSLTLTYKTPLAGTYDILVGY 146 AG I LEAEDG + G V+V + GYSG GYV S+ +++ P G Y ++ G Sbjct: 477 AGEIRLEAEDGELLG-VAVDSTLTGYSGRGYVTGFDAPEDSVRFSFEAP-RGVYRVVFGV 534 Query: 147 CAPNGEKKTSMVLNG-HTSEITLPETTGFIEISAGKTWMNQGDNTIEILANWGWYNIDYV 205 + ++ ++ H + + GF E S G+ W+++G +T+ G +DYV Sbjct: 535 SFSSRFASYALRVDDWHQTGSLIKRGGGFFEASIGEIWLDEGAHTMAFQLMNG--ALDYV 592 Query: 206 KLTAAAKPEQREVVSTLMNPDATPEARALMNFMDSQYGQKIISGQQT------LEDAEWI 259 +L + + L + AT A+AL F+ S+YG+ I++GQQ + ++ Sbjct: 593 RLEPVSYGPPARPPAQLSDSQATASAQALFAFLLSEYGRHILAGQQQNPYRRDFDAINYV 652 Query: 260 GQETGKYPAILATDLMDYSPSRVENGASSTEI-EKMIAWYNRGGIVSLCWHWNAPKGIGG 318 TGK PA+++ DL+DYSP+R +G + E IAW R GIVSL WHWNAP + Sbjct: 653 RNVTGKEPALVSFDLIDYSPTREAHGVVHYQTPEDWIAWAGRDGIVSLMWHWNAPTDLI- 711 Query: 319 EEPGYE--WWRGFYTEYTTFDVEYALDHPDSEDYHLLIRDIDEIAIQLKRLQNAGVPVLW 376 E+P + WW GFYT TTFDV AL SE Y LL+RDID IA QL++ Q A +PVLW Sbjct: 712 EDPSQDCYWWYGFYTRCTTFDVAAALADTSSERYRLLLRDIDVIAAQLQKFQQADIPVLW 771 Query: 377 RPLHEAEGGWFWWGAKGPEPAKRLWKLMVDRLTSHHHLNNLIWVWNSEK--PEWYPGDDV 434 RPLHEA GGWFWWGAKGPEP K+LW+L+ +RL HH L+NLIWV+ E EWYPGD Sbjct: 772 RPLHEAAGGWFWWGAKGPEPFKQLWRLLYERLVHHHGLHNLIWVYTHEPGAAEWYPGDAY 831 Query: 435 VDIASVDIYNPAGDYNPSI-AKYENLVSLVNGKKVIGLAENGPIPDPEMLQAYGADWSFF 493 VDI D+Y A D + + + + L +L G+K++ L E G +PD E++ YG WS+F Sbjct: 832 VDIVGRDVY--ADDPDALMRSDWNELQTLFGGRKLVALTETGTLPDVEVITDYGIWWSWF 889 Query: 494 TTWTGSFIKDGATNSAEHLQRIYKHDYVVTLDELP 528 + WT F++D + L R+Y + V+T DELP Sbjct: 890 SIWTDPFLRD---VDPDRLTRVYHSERVLTRDELP 921 >pir||JC7657 mannan endo-1,4-beta-mannosidase (EC 3.2.1.78) - Clostridium thermocellum dbj|BAB19050.1| mannanase 26B [Clostridium thermocellum] Length = 591 Score = 304 bits (779), Expect = 7e-81, Method: Compositional matrix adjust. Identities = 188/472 (39%), Positives = 264/472 (55%), Gaps = 41/472 (8%) Query: 89 SAGNILEAEDGMMSGG-VSVGTAGDGYSGEGY--------VIFQQSGSLTLTYKTPLAGT 139 S G+ E EDG+ G + G GE + I Q+ S ++ + G Sbjct: 33 SDGDKYEFEDGIHKGAQIYTDYVGQNEYGEVFDLTGSTCSFIAQKGTSTSVNVEVDKEGL 92 Query: 140 YDILVGYCAPNGEKKTSMVLNGH---TSEITLPETTGFIEISAGKTWMNQGDNTIEILAN 196 Y+I + Y P + K LN + EI+ P T + EISAG +N G N IE+ + Sbjct: 93 YEIFICYVQPYDKNKKVQYLNVNGVNQGEISFPFTLKWREISAGIVKLNAGINNIELESY 152 Query: 197 WGWYNIDYVKLTAAAKP-EQREVVSTLMNPDATPEARALMNFMDSQYGQKIISGQQTL-- 253 WG+ DY+ + A + + +V L+NP+AT EA+ALM+++ YG+ I+SGQQ + Sbjct: 153 WGYTYFDYLIVKPADESIVELKVPKKLVNPNATKEAKALMSYLVDIYGKHILSGQQEICG 212 Query: 254 ------EDAE--WIGQETGKYPAILATDLMDYSPSRVENGASSTEIEKMIAWYN-RGGIV 304 +AE +I ++TGK PA+ D M+Y + + E ++I WY +GGI Sbjct: 213 SHNYPGSEAEFTYIQEKTGKLPAVRGFDFMNYRGNGLMWDDQCAE--RVIEWYKEKGGIP 270 Query: 305 SLCWHWNAPKGIGGEEPGYEWWRGFYTEYTTFDVEYALDHPDSEDYHLLIRDIDEIAIQL 364 ++CWHW +P IG + FYTE TTF + AL P +E+ L+ DID IA +L Sbjct: 271 TVCWHWFSPGDIGKKAD-----NSFYTESTTFSISRAL-TPGTEENIALLNDIDTIARKL 324 Query: 365 KRLQNAGVPVLWRPLHEAEGGWFWWGAKGPEPAKRLWKLMVDRLTSHHHLNNLIWVWNSE 424 K++Q+AGVPVL+RPLHEAEGGWFWWGA+GPEP RL++L+ D+ T+ + LNNLIWVW S Sbjct: 325 KQVQDAGVPVLFRPLHEAEGGWFWWGAEGPEPCVRLYRLLYDKFTNEYGLNNLIWVWTSY 384 Query: 425 KPE----WYPGDDVVDIASVDIYNPAGDYNPS----IAKYENLVSLVNGKKVIGLAENGP 476 E WYPGDDVVDI D YN A D P+ + + NLV L NGKK++ + EN Sbjct: 385 DYETSAAWYPGDDVVDIIGYDKYN-AKDGKPNGSAISSTFYNLVKLTNGKKLVAMTENDT 443 Query: 477 IPDPEMLQAYGADWSFFTTWTGSFIKDGATNSAEHLQRIYKHDYVVTLDELP 528 IP L A W +F W G ++ N + L +Y+ DY +TLDELP Sbjct: 444 IPRVSNLVNEKAGWLYFCPWYGWWLTSEQNNPVDWLVEMYQSDYCITLDELP 495 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.315 0.134 0.399 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 1,707,756,015 Number of extensions: 75998762 Number of successful extensions: 183811 Number of sequences better than 1.0e-05: 186 Number of HSP's gapped: 183224 Number of HSP's successfully gapped: 227 Length of query: 1129 Length of database: 757,237,578 Length adjustment: 137 Effective length of query: 992 Effective length of database: 385,269,015 Effective search space: 382186862880 Effective search space used: 382186862880 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 132 (55.5 bits)