BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0591.0030.p (214 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_00127057.1| COG1335: Amidases related to nicotinamidase [... 310 1e-83 ref|NP_792778.1| isochorismatase family protein [Pseudomonas syr... 308 6e-83 gb|EAF82369.1| unknown [environmental sequence] 119 5e-26 ref|NP_355446.1| AGR_C_4541p [Agrobacterium tumefaciens] >gi|179... 117 2e-25 sp|P58760|YO99_AGRT5 Hypothetical isochorismatase family protein... 117 2e-25 >ref|ZP_00127057.1| COG1335: Amidases related to nicotinamidase [Pseudomonas syringae pv. syringae B728a] Length = 249 Score = 310 bits (795), Expect = 1e-83, Method: Compositional matrix adjust. Identities = 145/209 (69%), Positives = 173/209 (82%) Query: 2 KAKMKWDIQPERTAVIVVDMQNVFCKPEGALYVPRTEGIIGNIKELTGAARAAGMPVVYL 61 K ++W I P RTAVIVVDMQ VFC+P GALYV T I+ I+ L ARAA + VVYL Sbjct: 40 KPLVRWPINPLRTAVIVVDMQKVFCEPSGALYVKSTADIVQPIQMLLQTARAARVMVVYL 99 Query: 62 RHIVRGDGSDTGRMLDMYPNVNEILKRGTPGVEIIDELAPEPQDIIVDKLFYSGFHDTDL 121 RHIVRGDGSDTGRM D+YPNV++IL R PGVE+I+ LAP P D+IVDKLFYSGFH+TDL Sbjct: 100 RHIVRGDGSDTGRMRDLYPNVDQILARHDPGVEVIEALAPRPGDVIVDKLFYSGFHNTDL 159 Query: 122 DTILRVHDINTIIICGTVTNVCCETTVRDGAHREYKVIFLSDANAAMDYPDMGWGGISAE 181 DT+LR D++TII+CGTVTNVCCETT+RDG HREYKVI LSDANAAMDYPD+G+G +SAE Sbjct: 160 DTVLRARDVDTIIVCGTVTNVCCETTIRDGVHREYKVIALSDANAAMDYPDVGFGAVSAE 219 Query: 182 EIQRVTLTVVAYEFGQVSLTEDIIQEIQS 210 E+QR++LT +AYEFG+V+ T D+IQ I S Sbjct: 220 EVQRISLTTIAYEFGEVTTTADVIQRIAS 248 >ref|NP_792778.1| isochorismatase family protein [Pseudomonas syringae pv. tomato str. DC3000] gb|AAO56473.1| isochorismatase family protein [Pseudomonas syringae pv. tomato str. DC3000] Length = 227 Score = 308 bits (788), Expect = 6e-83, Method: Compositional matrix adjust. Identities = 143/208 (68%), Positives = 172/208 (82%) Query: 2 KAKMKWDIQPERTAVIVVDMQNVFCKPEGALYVPRTEGIIGNIKELTGAARAAGMPVVYL 61 K ++W I P RTAVIVVDMQ VFC+P GALYV T I+ ++ L AARAA + VVYL Sbjct: 18 KPLVQWPINPRRTAVIVVDMQKVFCEPHGALYVKSTADIVRPVQTLLCAARAAQVMVVYL 77 Query: 62 RHIVRGDGSDTGRMLDMYPNVNEILKRGTPGVEIIDELAPEPQDIIVDKLFYSGFHDTDL 121 RH VRGDGSDTGRM D+YPNV++IL R P VEIID LAPE D+I+DKLFYSGFH+TDL Sbjct: 78 RHTVRGDGSDTGRMRDLYPNVDQILARHDPDVEIIDALAPESGDVIIDKLFYSGFHNTDL 137 Query: 122 DTILRVHDINTIIICGTVTNVCCETTVRDGAHREYKVIFLSDANAAMDYPDMGWGGISAE 181 DT+LR D++TIIICGTVTNVCCETT+RDG HREYKVI LSDANAAMDYPD+G+G +SAE Sbjct: 138 DTVLRARDVDTIIICGTVTNVCCETTIRDGVHREYKVIALSDANAAMDYPDVGFGAVSAE 197 Query: 182 EIQRVTLTVVAYEFGQVSLTEDIIQEIQ 209 E+QR++LT +AYEFG+V+ T D+I+ I+ Sbjct: 198 EVQRISLTTIAYEFGEVTTTADVIRRIE 225 >gb|EAF82369.1| unknown [environmental sequence] Length = 237 Score = 119 bits (298), Expect = 5e-26, Method: Compositional matrix adjust. Identities = 74/225 (32%), Positives = 111/225 (49%), Gaps = 29/225 (12%) Query: 9 IQPERTAVIVVDMQNVFCKPEGALY-----VPRTEGIIGNIKELTGAARAAGMPVVYLRH 63 + P TAVIVVD+QN + G L V TE +I N ++ ARAAGMPVV+L++ Sbjct: 10 LNPSETAVIVVDLQNAYASKNGYLDKAGFDVSTTEPVIANTVKVLDTARAAGMPVVFLQN 69 Query: 64 IVRGDGSDTG-------------RMLDMYPNVN-EILKRGTPGVEIIDELAPEPQDIIVD 109 D + G + + P + +L +GT ++D L P+ DI++ Sbjct: 70 GWDADYKEAGGPGSPNWYKSNALKTMRKQPELKGSLLAKGTWDYALVDALKPQDGDIVIP 129 Query: 110 KLFYSGFHDTDLDTILRVHDINTIIICGTVTNVCCETTVRDGAHREYKVIFLSDANAAMD 169 K YSGF++T+LD++LR I ++ G TNVC E+T+RDG H EY + L+DA Sbjct: 130 KTRYSGFYNTNLDSMLRARGIRNLVFTGIATNVCVESTLRDGFHLEYFGVVLADAAYQAG 189 Query: 170 YPDMGWGGISAEEIQRVTLTVVAYEFGQVSLTEDIIQEIQSKTLS 214 PD I +L + FG VS T + + + + Sbjct: 190 PPD----------IHEASLFNIQTFFGWVSTTAHFCEVFEGASYA 224 >ref|NP_355446.1| AGR_C_4541p [Agrobacterium tumefaciens] ref|NP_533170.1| Isochorismatase [Agrobacterium tumefaciens str. C58 (U. Washington)] pir||F97659 probable synthetase (AE005300) [imported] - Agrobacterium tumefaciens (strain C58, Cereon) pir||AH2883 Isochorismatase [imported] - Agrobacterium tumefaciens (strain C58, Dupont) gb|AAK88231.1| AGR_C_4541p [Agrobacterium tumefaciens str. C58 (Cereon)] gb|AAL43486.1| Isochorismatase [Agrobacterium tumefaciens str. C58 (U. Washington)] Length = 246 Score = 117 bits (292), Expect = 2e-25, Method: Compositional matrix adjust. Identities = 73/215 (33%), Positives = 109/215 (50%), Gaps = 29/215 (13%) Query: 9 IQPERTAVIVVDMQNVFCKPEG-----ALYVPRTEGIIGNIKELTGAARAAGMPVVYLRH 63 ++P TAV+VVDMQN + G + +G I NIK+ AARAAG+ V+Y ++ Sbjct: 30 LKPSETAVVVVDMQNAYSTEGGYVDLAGFDISGAKGTIANIKKTLDAARAAGVQVIYFQN 89 Query: 64 IVRGDGSDTG-------------RMLDMYPNVN-EILKRGTPGVEIIDELAPEPQDIIVD 109 D + G + + P + ++L +GT I+DEL P+P DI+V Sbjct: 90 GWDKDYVEAGGPGSPNWHKSNALKTMRKRPELQGQLLAKGTWDYAIVDELQPQPGDILVP 149 Query: 110 KLFYSGFHDTDLDTILRVHDINTIIICGTVTNVCCETTVRDGAHREYKVIFLSDANAAMD 169 K YSGF +T++D++LR I ++ G TNVC E+++RD H EY + L DA + Sbjct: 150 KTRYSGFFNTNMDSVLRARGIRNLVFVGIATNVCVESSLRDAFHLEYFGVMLEDATHHLG 209 Query: 170 YPDMGWGGISAEEIQRVTLTVVAYEFGQVSLTEDI 204 PD IQ+ T+ V FG V+ D Sbjct: 210 -PDF---------IQQATVYNVEKFFGWVATVNDF 234 >sp|P58760|YO99_AGRT5 Hypothetical isochorismatase family protein ATu2499/AGR_C_4541 Length = 228 Score = 117 bits (292), Expect = 2e-25, Method: Compositional matrix adjust. Identities = 73/215 (33%), Positives = 109/215 (50%), Gaps = 29/215 (13%) Query: 9 IQPERTAVIVVDMQNVFCKPEG-----ALYVPRTEGIIGNIKELTGAARAAGMPVVYLRH 63 ++P TAV+VVDMQN + G + +G I NIK+ AARAAG+ V+Y ++ Sbjct: 12 LKPSETAVVVVDMQNAYSTEGGYVDLAGFDISGAKGTIANIKKTLDAARAAGVQVIYFQN 71 Query: 64 IVRGDGSDTG-------------RMLDMYPNVN-EILKRGTPGVEIIDELAPEPQDIIVD 109 D + G + + P + ++L +GT I+DEL P+P DI+V Sbjct: 72 GWDKDYVEAGGPGSPNWHKSNALKTMRKRPELQGQLLAKGTWDYAIVDELQPQPGDILVP 131 Query: 110 KLFYSGFHDTDLDTILRVHDINTIIICGTVTNVCCETTVRDGAHREYKVIFLSDANAAMD 169 K YSGF +T++D++LR I ++ G TNVC E+++RD H EY + L DA + Sbjct: 132 KTRYSGFFNTNMDSVLRARGIRNLVFVGIATNVCVESSLRDAFHLEYFGVMLEDATHHLG 191 Query: 170 YPDMGWGGISAEEIQRVTLTVVAYEFGQVSLTEDI 204 PD IQ+ T+ V FG V+ D Sbjct: 192 -PDF---------IQQATVYNVEKFFGWVATVNDF 216 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.320 0.138 0.409 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 314,645,174 Number of extensions: 13200760 Number of successful extensions: 29786 Number of sequences better than 1.0e-05: 291 Number of HSP's gapped: 29542 Number of HSP's successfully gapped: 291 Length of query: 214 Length of database: 757,237,578 Length adjustment: 120 Effective length of query: 94 Effective length of database: 431,425,698 Effective search space: 40554015612 Effective search space used: 40554015612 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 123 (52.0 bits)