BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0591.0027.p (413 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_721657.1| putative permease; multidrug efflux protein [St... 167 6e-40 ref|NP_350092.1| Predicted MDR-type permease [Clostridium acetob... 152 1e-35 ref|NP_765793.1| TcaB protein [Staphylococcus epidermidis ATCC 1... 147 4e-34 ref|NP_629049.1| putative transport integral membrane protein [S... 85 3e-15 ref|ZP_00019946.1| hypothetical protein [Chloroflexus aurantiacus] 82 3e-14 >ref|NP_721657.1| putative permease; multidrug efflux protein [Streptococcus mutans UA159] gb|AAN58963.1| putative permease; multidrug efflux protein [Streptococcus mutans UA159] Length = 392 Score = 167 bits (422), Expect = 6e-40, Method: Compositional matrix adjust. Identities = 117/357 (32%), Positives = 174/357 (48%), Gaps = 4/357 (1%) Query: 26 NYRMISILLFFPALFILSALYITVPLIPVFAGEFHISLEQXXXXXXXXXXXXXXXXXXXX 85 N ++ILLF+ L ++++ YIT+PL+ F +FH+S EQ Sbjct: 9 NEISLAILLFWCGLIVVASNYITIPLMSAFTQQFHVSAEQTAWLGTSFSFAYALSCLLVG 68 Query: 86 PLSDKLGRKRVMXXXXXXXXXXXXXXXXXXXFPALIVFRCIQGAVAATFSPVALTYAGEV 145 LSD+ GRKR+M LI+ R +QG A++F+PVA+ Y E Sbjct: 69 VLSDRFGRKRIMVGGLILLSVITLIIPFGNQLSWLIMGRIMQGIAASSFAPVAVVYISEK 128 Query: 146 FPTHRRVSAIGMISAGFLMAGILGQVWASYLNERLGWHAVFLLLAAVYGLTFXXXXXXXX 205 F + + +G++SA FL++ I+GQ+ ++Y++ L W AVF +Y LT Sbjct: 129 FSKEIKGTVVGLVSASFLISAIVGQIMSAYISRLLNWQAVFCTFGIIYLLTALILYSYAS 188 Query: 206 XXXXXXXXXXTPGSRRTEWRSILANRYLWLCYSVAVMLLLCFVGMYTVLGSF-LHNSPYE 264 R W IL + L Y +A+ LL FV YTVL + +H + Sbjct: 189 PVSNQSKGILFSQLFRHYWE-ILRKKNLIKLYLIALTLLFAFVAFYTVLEGYSVHT--FH 245 Query: 265 LSNMQVLGIRAFGMIGMLCCFISGPCCARWGSKAVLRSGLLLAIAAMCALSLVRSIAAYT 324 LS + IRA G+IGML + ++ K +L LL + ++ ++ + Sbjct: 246 LSKDNIFKIRALGVIGMLFAPFADKLARKFDIKCLLILSLLFSFVSLFIIAFCPYLNVLI 305 Query: 325 ALSAVFVAGIAILVPSLISLVGDIGSKQRATATSLYTFILFAGASLGPIVSVYALDI 381 +S +FVAGI+I+VP+LI LVG G Q A S YTFILF GAS GPI V L + Sbjct: 306 IMSLLFVAGISIIVPTLIILVGRFGQNQAGIAVSFYTFILFLGASFGPIAGVQLLTV 362 >ref|NP_350092.1| Predicted MDR-type permease [Clostridium acetobutylicum] pir||E97330 probable MDR-type permease [imported] - Clostridium acetobutylicum gb|AAK81432.1| Predicted MDR-type permease [Clostridium acetobutylicum] Length = 392 Score = 152 bits (384), Expect = 1e-35, Method: Compositional matrix adjust. Identities = 117/396 (29%), Positives = 191/396 (48%), Gaps = 9/396 (2%) Query: 13 MSKLTTNPPWIHSNYRMISILLFFPALFILSALYITVPLIPVFAGEFHISLEQXXXXXXX 72 ++K + IH YR+ I LF ++ + + +Y +PL V FH+ EQ Sbjct: 2 LNKESLECEGIH--YRLQVIFLFIASICVGTGIYEWIPLTNVLQQSFHVGQEQIIFIGSV 59 Query: 73 XXXXXXXXXXXXXPLSDKLGRKRVMXXXXXXXXXXXXXXXXXXXFPALIVFRCIQGAVAA 132 L++ + K+++ + +IVFR IQG AA Sbjct: 60 FSLFYAIGFLIFGWLANYVEIKKLLIVGTLLLMISTFLIGFATNYKLMIVFRAIQGIFAA 119 Query: 133 TFSPVALTYAGEVFPTHRRVSAIGMISAGFLMAGILGQVWASYLNERLGWHAVFLLLAAV 192 +F+P+ + ++FP +R++ I I+ GF+MAGILGQ++ S + W VF L A + Sbjct: 120 SFAPLGFSIIVKMFPESKRIAGISAITTGFVMAGILGQLYGSIIANSYSWSVVFWLQALI 179 Query: 193 YGLTFXXXXXXXXXXXXXXXXXXTPGSRRTEWRSILANRYLWLCYSVAVMLLLCFVGMYT 252 Y P S E +L ++ L+ CY + + LL CFVGMYT Sbjct: 180 Y---LMIIISMIFLVPKTDKVSERPTSFIGELVKLLISKSLFPCYVITLTLLFCFVGMYT 236 Query: 253 VLGSFLHNSPYELSNMQVLGIRAFGMIGMLCCFISGPCCARWGSKAVLRSGLLLAIAAMC 312 V+G F + L+N ++L IR G+IGM+ + + G +L GL+ A + Sbjct: 237 VMGMFFQKD-FGLNNEKILWIRGIGIIGMIGSMVFAKLSKKIGVGNILSLGLVCASIGVL 295 Query: 313 ALSLVRSIAAYTALSAVFVAGIAILVPSLISLVGDIGSKQRATATSLYTFILFAGASLGP 372 + + ++ A+S VFV GI I +P ++S +G + K+ A +LYTF+LF GA++GP Sbjct: 296 IIGISNNVGLSVAMSVVFVFGITISLPMVVSSIGTLAGKKSGNAMTLYTFVLFIGATVGP 355 Query: 373 IVSVYALDIGP-PNVLPFLIFAGLLTVGLINSLFIK 407 IV ++ G P L F+ A +L + I SL ++ Sbjct: 356 IVCNRIIESGSYP--LAFIFLAVVLGISFIASLIVR 389 >ref|NP_765793.1| TcaB protein [Staphylococcus epidermidis ATCC 12228] gb|AAO05880.1| TcaB protein [Staphylococcus epidermidis ATCC 12228] Length = 390 Score = 147 bits (371), Expect = 4e-34, Method: Compositional matrix adjust. Identities = 101/393 (25%), Positives = 184/393 (46%), Gaps = 16/393 (4%) Query: 25 SNYRMISILLFFPALFILSALYITVPLIPVFAGEFHISLEQXXXXXXXXXXXXXXXXXXX 84 NY +I+ ++F + ++++LY +PL FA +FH+ Sbjct: 5 KNYNLITTIMFISGIIVMASLYTALPLTATFAEDFHVPESIATLNGVLFSLTYSISCLFY 64 Query: 85 XPLSDKLGRKRVMXXXXXXXXXXXXXXXXXXXFPALIVFRCIQGAVAATFSPVALTYAGE 144 +S+K GR + + F L++ R +QG AA+FSPV +TY E Sbjct: 65 GTISEKYGRIKTILVGMSALVIICLMIGIVHSFTVLLILRALQGVFAASFSPVVMTYTTE 124 Query: 145 VFPTHRRVSAIGMISAGFLMAGILGQVWASYLNERLGWHAVFLLLAAVYGLTFXXXXXXX 204 +P +RV+AI IS F+++G+LGQ + + L W V+ +L +Y Sbjct: 125 TYPRVKRVTAISFISTSFMLSGVLGQNMSELVVSYLNWQWVYFILTILY-----LILVLV 179 Query: 205 XXXXXXXXXXXTPGSRR----TEWRSILANRYLWLCYSVAVMLLLCFVGMYTVLGSFL-- 258 P + ++ N ++ C +++ LL+ F+ MY +L ++ Sbjct: 180 IYKNVPESPHKNPDIQLIKFFNNFKDFKDNLKVFYCLFISLTLLIMFISMYDILNEYVTS 239 Query: 259 HNSPYELSNMQVLGIRAFGMIGMLCCFISGPCCARWGSKAVLRSGLLLAIAAMCALSLVR 318 H ++S ++ + FG+IGML ++G +R G K +L L I ++ + + Sbjct: 240 HQVGGDMSVSSMM--KLFGVIGMLLSLLAGRVSSRIGIKRLLTIALTTCIVSIILMGVTT 297 Query: 319 SIAAYTALSAVFVAGIAILVPSLISLVGDIGSKQRATATSLYTFILFAGASLGPIVSVYA 378 +I T S +FVAGIA +P++IS +G + + S+ TF+LF G ++ PI+ ++ Sbjct: 298 NIILITTFSVLFVAGIAFAIPTVISKIGVVVKNNQGFFLSVNTFVLFLGTAIAPILMLFV 357 Query: 379 LDIGPPNVLPFLIFAGLLTVGLINSLFIKLPGR 411 ++ L FL+ A + + L+ S+F +PG Sbjct: 358 GELSNF-FLQFLMIAIVGLIALVVSIF--MPGD 387 >ref|NP_629049.1| putative transport integral membrane protein [Streptomyces coelicolor A3(2)] emb|CAC33061.1| putative transport integral membrane protein [Streptomyces coelicolor A3(2)] Length = 436 Score = 84.7 bits (208), Expect = 3e-15, Method: Compositional matrix adjust. Identities = 91/368 (24%), Positives = 136/368 (36%), Gaps = 23/368 (6%) Query: 26 NYRMISILLFFPALFILSALYITVPLIPVFAGEFHISLEQXXXXXXXXXXXXXXXXXXXX 85 YR +S LF + + LY T L+P+ +G+ ++ Sbjct: 38 GYRRMSFALFLAGVATFALLYSTQALLPLISGDLGVTASDASWTVAAATGGLALFVLPMS 97 Query: 86 PLSDKLGRKRVMXXXXXXXXXXXXXXXXXXXFPALIVFRCIQGAVAATFSPVALTYAGEV 145 LS++ GR+ VM PAL+ R +QGA A A Y E Sbjct: 98 ALSERFGRRTVMTASLAVAVSVGLLVPFAPSLPALVALRAVQGAALAGLPASATAYLAEE 157 Query: 146 FPTHRRVSAIGMISAGFLMAGILGQVWASYLNERLGWH---AVFLLLAAVYGLTFXXXXX 202 V+AIG+ AG + G+ G+V ++ + GW V L+A + F Sbjct: 158 VRPRALVTAIGLFVAGNSVGGMSGRVITGWVAQEWGWRVAVGVIGLVAVACAVAF----- 212 Query: 203 XXXXXXXXXXXXXTPGSRRTE-----WRSILANRYLWLCYSVAVMLLLCFVGMYTVLGSF 257 TPGS R R LAN L Y++ + + F G+YTV+G Sbjct: 213 ---RLLLPAPQHFTPGSLRPRVLVGTVRDHLANPLLRRLYAIGALFMTVFGGVYTVIGYR 269 Query: 258 LHNSPYELSNMQVLGIRAFGMIGMLCCFISGPCCARWGSKAVLRSGLLLAIAAMCALSLV 317 L +P+ L + I ++G + +G R G + L G A + Sbjct: 270 LTEAPFSLPQGLIGSIFLVYLVGTVSASTAGRLVGRLGRRGALYLGAGTTAAGLLLSLAP 329 Query: 318 RSIAAYTAL---SAVFVAGIAILVPSLISLVGDIGSKQRATATSLYTFILFAGASLGPIV 374 L +A F AG A+ S VG RA A++LY + G+S G V Sbjct: 330 SLPMVLLGLVLITAGFFAGHAVAS----SAVGKTAQHGRAQASALYQSAYYVGSSAGSTV 385 Query: 375 SVYALDIG 382 A G Sbjct: 386 GAVAFHSG 393 >ref|ZP_00019946.1| hypothetical protein [Chloroflexus aurantiacus] Length = 386 Score = 81.6 bits (200), Expect = 3e-14, Method: Compositional matrix adjust. Identities = 80/358 (22%), Positives = 159/358 (44%), Gaps = 19/358 (5%) Query: 25 SNYRMISILLFFPALFILSALYITVPLIPVFAGEFHISLEQXXXXXXXXXXXXXXXXXXX 84 ++ R +I L+ + + S +Y+T P++P + F+++ Sbjct: 7 TSRRTWTIALYCAGVIVFSDMYLTQPILPQLSVTFNVAPATAGLTVSVVVLMIALGSLAV 66 Query: 85 XPLSDKLGRKRVMXXXXXXXXXXXXXXXXXXXFPALIVFRCIQGAVAATFSPVALTYAGE 144 PLSD+LGR+ VM F L+ +R +QG + VA+ Y G+ Sbjct: 67 GPLSDRLGRQPVMAGSCLLLGLPTLLCALAPSFELLLFWRALQGLCIPGLTAVAVAYLGD 126 Query: 145 VFPTHRRVSAIGMISAGFLMAGILGQVWASYLNERLGWHAVFLLLAAVYGLTFXXXXXXX 204 P A+G A + G+ G+V+ +++ LGW F++ A GLT Sbjct: 127 HLPPSALGRAVGGWIAANVAGGLSGRVFGGLISDSLGWRMSFIVFA---GLTLASGIGLL 183 Query: 205 XXXXXXXXXXXTPGSRRTEWRSILA---NRYLWLCYSVAVMLLLCFVGMYTVLGSFLHNS 261 G + +R+++A +R L Y +A L F+G++T L +L+ Sbjct: 184 LTLPRDQPSSV--GGWQQAYRAMIAHLYDRQLRYAYLLAGTLFFGFLGIFTYLPYYLNAP 241 Query: 262 PYELSNMQVLGIRAFGMIGMLCCFISGPCCARWGSKAVLRSGLLLAIAAMCALSLVRSIA 321 P++L V + G++ +G AR+ + ++ GLL+A++ + AL+L+ ++ Sbjct: 242 PFQLPPSIVALAYISYLAGVIVSPWAGSLSARYPRQRLIALGLLIAMSGI-ALTLIPNLP 300 Query: 322 A-----YTALSAVFVAGIAILVPSLISLVGDIGSKQRATATSLYTFILFAGASLGPIV 374 +T + +F+A + P+L++ + + + A++LY + G +LG ++ Sbjct: 301 VIALGLWTLCTGMFIA--QSIAPALVN---QLAQQAKGAASALYLVAYYIGGTLGGVI 353 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.331 0.142 0.447 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 486,731,775 Number of extensions: 16989184 Number of successful extensions: 67168 Number of sequences better than 1.0e-05: 108 Number of HSP's gapped: 67388 Number of HSP's successfully gapped: 110 Length of query: 413 Length of database: 757,237,578 Length adjustment: 128 Effective length of query: 285 Effective length of database: 409,704,906 Effective search space: 116765898210 Effective search space used: 116765898210 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.9 bits) S2: 127 (53.5 bits)