BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0591.0013.p (612 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gb|AAC98125.1| peripheral protein [Geobacillus stearothermophilus] 161 5e-38 ref|NP_241930.1| BH1064~unknown conserved protein [Bacillus halo... 144 6e-33 ref|NP_241348.1| lipoprotein [Bacillus halodurans] >gi|25328392|... 65 4e-09 ref|NP_693012.1| hypothetical protein OB2091 [Oceanobacillus ihe... 65 6e-09 ref|ZP_00036085.1| COG1653: ABC-type sugar transport system, per... 60 1e-07 >gb|AAC98125.1| peripheral protein [Geobacillus stearothermophilus] Length = 493 Score = 161 bits (407), Expect = 5e-38, Method: Compositional matrix adjust. Identities = 139/470 (29%), Positives = 219/470 (46%), Gaps = 49/470 (10%) Query: 150 YNQKVSLAIASGD-LPDAMVVGAVEL-KEMVEADQLADLTEVYNTYASPVIKRIVDSTGG 207 Y+ K+ LA++SG LPD V +L +++ + ++ + E YASP +K+I + Sbjct: 50 YHNKIRLALSSGQKLPDVFKVSDGQLINDLIRSGKVMPVDEAIEKYASPRLKKIYEQFP- 108 Query: 208 EALGSVTFNGKILALPNVQLQADGIHXXXXXXXXXXXXXXXPPQTMEELEAVAHAFVEQD 267 EA T +GK +P PP+T+E+LE + AFV +D Sbjct: 109 EAFYPATVDGKRYGIPRFSGGNGSDSLLWIRKDWLDKLGLQPPKTIEDLEKIMDAFVNKD 168 Query: 268 PDGNGQRDTIGLAGPSATGRMYANFLQSTNNLYGFDG--IFAAYNAY-PGYWLEN-NGKV 323 PDGNG++DTIGL S G A +L DG IF AY Y PG W + +GK+ Sbjct: 169 PDGNGKKDTIGLTLASKNG--LATWLA--------DGSFIFGAYGNYEPGSWSKGEHGKL 218 Query: 324 VYGSIQPETKEALGILRKMYLSGAIDPEMGVREDPG--ESVASGKTGMFFAPWWAPYGPI 381 VYGS+QP K+AL L + Y G +D E+G+ ++ +S +GK+G+ AP WA PI Sbjct: 219 VYGSVQPSMKKALAKLNEWYQKGYLDKEVGILDEQTAIKSFVAGKSGIISAPPWAAGWPI 278 Query: 382 DDAVRNNPEANWQAYALPLDKSGSYRPHMSNTTSQFVVVRKGYEHPEAAMLIVNNLVLGE 441 DA++NNP A + Y LP G + + K ++H + ++ Sbjct: 279 TDALKNNPGAVVEPYPLPSGPDGKIGRRGEGLVTGMFLFSKDFKHMDKFFQYLDE----- 333 Query: 442 SRGTFTTERGPTEYPLRLTYAAADETEYEVKALRQVFAGTKTAEDFLNKPEYKMLQSDIR 501 + ++Y Y A+ +Y +K + V+ + L+ Y + ++ Sbjct: 334 ---AYAYVYNDSKY---FKYGLAEGYDYVMKNGKPVYDDKEIPGGRLDPGAYFITEA--- 384 Query: 502 NIGPVKLGPYDKYDI------QYWEPDKNRAMWVRAYSL---MVGGSPLVDNEIKGIFSA 552 + PY YD+ +P KN + R SL + + +V+ + + Sbjct: 385 ----IPTVPYMLYDLVEELYTTKRQP-KNAYEYTRIVSLGESFMKAATIVNQQNQYRIEN 439 Query: 553 TYTQTP--TMESRWLKLQRLEDETFLKIILGAAPLEAFDHFAEEWKRQGG 600 +T P TM+ R L ++E ETF II G APL AFD F ++W+ GG Sbjct: 440 EFTGPPTKTMQKRGEFLTKMERETFANIIYGRAPLSAFDDFVKKWEESGG 489 >ref|NP_241930.1| BH1064~unknown conserved protein [Bacillus halodurans] pir||H83782 hypothetical protein BH1064 [imported] - Bacillus halodurans (strain C-125) dbj|BAB04783.1| BH1064~unknown conserved protein [Bacillus halodurans] Length = 550 Score = 144 bits (363), Expect = 6e-33, Method: Compositional matrix adjust. Identities = 155/587 (26%), Positives = 256/587 (43%), Gaps = 76/587 (12%) Query: 55 MKRRL---GMIAVLLISLTTNACSNSKNSNSDASQHAKLLPGSSGKYEEPVTIHIGTTVE 111 MK+R+ ++ +L + + T ACS ++ D + + + VTI T++ Sbjct: 1 MKKRIVHSFLVGLLAVGILT-ACSQEGSTTEDETNTNE---------DGTVTITTARTLD 50 Query: 112 STKSLLQGETLVDNQYTRNIKNNLNI-IVDYSFKASPANYNQKVSLAIASGD-LPDAMVV 169 GE + DN TR + L I V + + +N ++ L ++SG+ LPD V Sbjct: 51 DGTVFKDGEDVHDNVVTRWAEEELGIKFVTEWTQPNDEQFNTQLRLMMSSGEKLPDVFQV 110 Query: 170 GAVELK-EMVEADQLADLTEVYNTYASPVIKRIVDSTGGEALGSVTFNGKILALPNVQLQ 228 +K +++ + + + E TYASP +K++ + +A T +G +P Sbjct: 111 NDTAIKADLLSSGLVMPVDEAIETYASPRLKQLFEQFP-QAFYPSTKDGVRYGIPRFSGG 169 Query: 229 ADGIHXXXXXXXXXXXXXXXPPQTMEELEAVAHAFVEQDPDGNGQRDTIGLAGPSATGRM 288 PP T+EELE + FV +DP+ G+ DTIG+ + Sbjct: 170 NGSDTLLWIRQDWLDKFDLEPPTTLEELEHIMDVFVNEDPNETGKNDTIGITFAAGDYGF 229 Query: 289 YANFLQSTNNLYGFDGIFAAYNAYPGYWLE-NNGKVVYGSIQPETKEALGILRKMYLSGA 347 + ++ ++G G F PG W + ++G +VYGSIQP K+ L + + Y G Sbjct: 230 RGGNIADSSWVFGAFGDFV-----PGAWSKGDDGSLVYGSIQPSMKDGLAKIHEWYEKGY 284 Query: 348 IDPEMGVRE--DPGESVASGKTGMFFAPWWAPYGPIDDAVRNNPEANWQAYALPLDKSGS 405 + + + D ES SG++G+ AP WA PI + + P+A + + Sbjct: 285 LATDFAILSAGDAQESFISGRSGIMAAPPWAHGYPIGEMAQVVPDAIVKPF--------- 335 Query: 406 YRPHMSNTTSQ-------FVV----VRKGYEHPEAAMLIVNNLVLGESRGTFTTERGPTE 454 PH+S Q +VV K +EH + + + G + G E E Sbjct: 336 --PHVSGPNGQAGRRGEGYVVGSFLFNKDFEHMD-KFFEYWDAIYGYTFG----ESDYFE 388 Query: 455 YPL--RLTYAAAD-ETEYEVKALRQVFAGTKTAEDFLNKPEYKMLQSDIRNIGPVKLGPY 511 Y L Y D E Y+ ++ T E+ ++ Y L +++ I P+ Sbjct: 389 YGLFEGYDYVMEDGEPVYDHAQFEEI-----TGEEVVDPGRY-FLPTNVPTI------PF 436 Query: 512 DKYDIQYWEPDKNRAMWVRAYSLMVGGSP-------LVD--NEIKGIFSATYTQTPTMES 562 + Y + + R + + G P +V+ N+++ I TPTM S Sbjct: 437 EMYSLLEEFHETGREPETGYEAGLAGRDPDYIEAAAIVNRQNDLRIINEFNGPPTPTMVS 496 Query: 563 RWLKLQRLEDETFLKIILGAAPLEAFDHFAEEWKRQGGEEITREVNE 609 R L+R+EDE F+ II G PL+AFD F EEW++ GGE IT EVNE Sbjct: 497 RSEHLERMEDELFVDIIYGNKPLDAFDQFVEEWRQSGGEAITEEVNE 543 >ref|NP_241348.1| lipoprotein [Bacillus halodurans] pir||B83710 lipoprotein BH0482 [imported] - Bacillus halodurans (strain C-125) dbj|BAB04201.1| lipoprotein [Bacillus halodurans] Length = 512 Score = 65.5 bits (158), Expect = 4e-09, Method: Compositional matrix adjust. Identities = 110/492 (22%), Positives = 183/492 (37%), Gaps = 115/492 (23%) Query: 153 KVSLAIASGDLPDAMVVGAVELKEMVEADQLADLTEVYNTYASPVIKRI--VDSTGGEAL 210 +V+ A+ASG+LP + + ++ A + E+ P + + L Sbjct: 95 RVNAALASGNLPKVITLQDFRTSSVLNAFRSGQFWEI-----GPYLDEFPNLSEMNETIL 149 Query: 211 GSVTFNGKI--------LALPNVQLQADGIHXXXXXXXXXXXXXXXPPQTMEELEAVAHA 262 +V+ +GKI L+ V ++ D + P +EEL VA A Sbjct: 150 ENVSVDGKIYGIYRERPLSRQGVVIRQDWLENLGLEM----------PTNLEELYEVARA 199 Query: 263 FVEQDPDGNGQRDTIGLAGPSATGRMYANFLQSTNNLYGFDGIFAAYNAYPGYW-LENNG 321 F DP+G+G DT+G F + ++G A+Y+ P W ++ G Sbjct: 200 FTYDDPNGSGADDTVG-------------FTDRNDLIFGAFKTLASYHGTPNEWGFDDEG 246 Query: 322 KVVYGSIQPETKEALGILRKMYLSGAIDPEMGV--REDPGESVASGKTGMFFAPWWAPYG 379 K++ + E E + ++ +Y G I+ E V +E E +G+ G++ G Sbjct: 247 KLMPEFMTDEYVETMNYMKSLYDEGLINTEFPVTSKEQQQELFMTGRAGIYV-------G 299 Query: 380 PIDDAVRNNPEANWQAYALPLDKSGSYRPHMSNTTSQFVVVRKGYEHPEAAMLIVNNLVL 439 + DA RN DK+ P M + + G + A Sbjct: 300 NMVDA-RN-----------LRDKAKDINPEMEVAITNRIAGPDGEDRVWAQ--------- 338 Query: 440 GESRGTFTTERGPTEYPLRLTYAAADETEYEVKALRQVFAGTKTAEDFLNKPEYKMLQSD 499 G G F + ETE E+K + F + AE+ + Y ++ Sbjct: 339 GGHNGIFAIPKSSV------------ETEAELKRILAFF--DRIAEEDV----YNLMAYG 380 Query: 500 IRNIGPVKLGPYDKYDIQYWEP-DKNRAMWVRAYSLMVG------------GSPLVD--- 543 I ++ Y+ D +Y+E ++N W +V G PL + Sbjct: 381 IEDVH------YETIDGEYFERIEENLDDWAAEVQPLVSLVAREMNYLKAYGDPLYEEYE 434 Query: 544 ------NEIKGIFSATYTQTPTMESRWLKLQRLEDETFLKIILGAAPLEAFDHFAEEWKR 597 NEI A +PT R +LQ++ D+ K ILG + F+ E W Sbjct: 435 RLTEDNNEIIVTNPAEPLFSPTYTERGTELQKIIDDATYKYILGELDEDGFEAEVERWLT 494 Query: 598 QGGEEITREVNE 609 GGE I E+NE Sbjct: 495 NGGESIIEELNE 506 >ref|NP_693012.1| hypothetical protein OB2091 [Oceanobacillus iheyensis HTE831] dbj|BAC14047.1| hypothetical conserved protein [Oceanobacillus iheyensis HTE831] Length = 504 Score = 64.7 bits (156), Expect = 6e-09, Method: Compositional matrix adjust. Identities = 57/231 (24%), Positives = 103/231 (44%), Gaps = 33/231 (14%) Query: 149 NYNQKVSLAIASGDLPDAMVVGA----VELKEMVEADQLADLTEVYNTYASPVIKRI--V 202 NY +++ A A+ LP+A+ + ++ K+ + DQ ++ P ++ + Sbjct: 81 NYEDRLNTAFATNTLPEAVFMKNQTTFMQFKDAIRDDQFWEV--------GPYLEEFENL 132 Query: 203 DSTGGEALGSVTFNGKILALPNVQ-LQADGIHXXXXXXXXXXXXXXXPPQTMEELEAVAH 261 + E L + +GKI +L + L GI P T +E +A Sbjct: 133 GNLKEEVLENTKVDGKIYSLYQGRPLSRQGI---IYRKDWADNLGLSAPTTTDEFMEMAR 189 Query: 262 AFVEQDPDGNGQRDTIGLAGPSATGRMYANFLQSTNNLYGFDGIFAAYNAYPGYWLENNG 321 AF E DPDGNG+ DT GL S +YG +++ P W E +G Sbjct: 190 AFTEDDPDGNGKDDTFGLTDRSDL-------------VYGAFKTVSSWFGTPNNWGEQDG 236 Query: 322 KVVYGSIQPETKEALGILRKMYLSGAIDPEMGV--REDPGESVASGKTGMF 370 ++V + PE K+A+ ++M+ +G I+ + V + D E + +G +G++ Sbjct: 237 ELVPEFMFPEYKQAMDFFKEMHENGYINKDFPVTSKVDQQEFIKNGSSGIY 287 >ref|ZP_00036085.1| COG1653: ABC-type sugar transport system, periplasmic component [Enterococcus faecium] Length = 502 Score = 60.5 bits (145), Expect = 1e-07, Method: Compositional matrix adjust. Identities = 39/124 (31%), Positives = 59/124 (47%), Gaps = 14/124 (11%) Query: 250 PQTMEELEAVAHAFVEQDPDGNGQRDTIGLAGPSATGRMYANFLQSTNNLYGFDGIFAAY 309 P TM+EL VA F E DPDGNG DT+G + + Y +F +F +Y Sbjct: 175 PTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSF-----------KLFTSY 223 Query: 310 NAYPGYW-LENNGKVVYGSIQPETKEALGILRKMYLSGAIDPEMGV--REDPGESVASGK 366 P W +++NGK E +AL R ++ +G + + V + D E A G+ Sbjct: 224 FGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQEQFAQGR 283 Query: 367 TGMF 370 TG++ Sbjct: 284 TGIY 287 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.315 0.133 0.390 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 905,801,577 Number of extensions: 38117516 Number of successful extensions: 65305 Number of sequences better than 1.0e-05: 9 Number of HSP's gapped: 65298 Number of HSP's successfully gapped: 10 Length of query: 612 Length of database: 757,237,578 Length adjustment: 132 Effective length of query: 480 Effective length of database: 398,844,510 Effective search space: 191445364800 Effective search space used: 191445364800 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 129 (54.3 bits)