BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0591.0011.p (522 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gb|AAQ91304.1| methyl-accepting chemotaxis protein histidine kin... 149 1e-34 ref|NP_561490.1| two-component sensor histidine kinase [Clostrid... 147 5e-34 ref|NP_607635.1| putative histidine kinase [Streptococcus pyogen... 135 3e-30 ref|NP_623881.1| predicted ATPase [Thermoanaerobacter tengcongen... 134 5e-30 ref|NP_388576.1| similar to two-component sensor histidine kinas... 133 1e-29 >gb|AAQ91304.1| methyl-accepting chemotaxis protein histidine kinase [Paenibacillus sp. Dex70-34] Length = 574 Score = 149 bits (377), Expect = 1e-34, Method: Compositional matrix adjust. Identities = 92/335 (27%), Positives = 170/335 (50%), Gaps = 14/335 (4%) Query: 162 DKRIAYSDVPEDFAMDDYFADSGSSGDAGLQNGYYWFESTSNQGWSVISLIPVNEYNQQK 221 +KR+ +D+ + +D+ +GD YY + + GW ++ +PV+E + Sbjct: 231 NKRVDNADLLRNITANDFVEFEFMNGDVR----YYGVKQRLSNGWLIVGTVPVHEITDKL 286 Query: 222 NRWVIQMIYXXXXXXXXXXXIAWFLWKMVYVPLQQFNHQIKSIAQRNTAVTEVTRIXXXX 281 + ++ I + + P++Q Q+K Q + +V RI Sbjct: 287 DSLHRNILGSFAAFTLAGILIGLVIANRITRPIKQLTRQMKMAQQGDLSV----RINVLS 342 Query: 282 XPEFDFLLKQLQNMKNQIAELIQEVEVKEKGRADLEVEKLLYQINPHFLMNTLDTVHWLA 341 EF + +Q M I L+ +V++++K + D E+ + ++INPHFL NTL + WL Sbjct: 343 KDEFGLMSRQFNRMMADIERLMDQVKLEQKQKRDAEIRAVTHRINPHFLFNTLSNLRWLI 402 Query: 342 VMNGQKDIDKLVVSLNKLLYYNLGKRGLVSTIREEIDSIRQYLTLQQVRYDFGFDVRIHV 401 N + + +L +LL N+GK+G T+ EE+D + +YL + ++RY+ F + +V Sbjct: 403 KYNQSAKAYEGISALIRLLEANMGKKGNFITVEEELDIVEKYLAILELRYNRTFRLHTNV 462 Query: 402 DDDVLELPIPRFILQPVLENSLYHGL---HDEGYIQVEVGKEEGQIIISVYDNGAGISAE 458 D E IPR ++QP++ENS++HG+ +G I ++ +E +II+V DNG GI + Sbjct: 463 DPAAAEFVIPRMLIQPLVENSVFHGIVPKETDGEIHIDAAMQEDALIITVRDNGIGILPD 522 Query: 459 E-IAKLLHQQQVEQQKAGMGIGMSYVKRMIENQYG 492 + + ++ + V ++ GIG+ +V I YG Sbjct: 523 KRVVEVSIKDAVLSGQS--GIGLRHVYECINLYYG 555 >ref|NP_561490.1| two-component sensor histidine kinase [Clostridium perfringens] dbj|BAB80280.1| two-component sensor histidine kinase [Clostridium perfringens str. 13] Length = 529 Score = 147 bits (372), Expect = 5e-34, Method: Compositional matrix adjust. Identities = 99/353 (28%), Positives = 182/353 (51%), Gaps = 21/353 (5%) Query: 170 VPEDFAMDDYFADSGSSGDAG--LQNGYYW---FESTSNQGWSVISLIPVNEYNQQKNRW 224 + E+ ++ + D G+ A + NG + + W + S+ P + N Sbjct: 187 ILEETDLNKFLKDKGAKDTARSIVVNGENYVATYRKLKGFNWYIASMTPYDFINADIQSL 246 Query: 225 VIQMIYXXXXXXXXXXXIAWFLWKMVYVPLQQFNHQIKSIAQRNTAVTEVTRIXXXXXPE 284 +M ++FL+ + VPL +++K +++ + V + E Sbjct: 247 RKEMTIIGLLFTLVVLIFSYFLYSSIKVPLYNLLNKMKRVSEGDLKVL----VNDTYEDE 302 Query: 285 FDFLLKQLQNMKNQIAELIQEVEVKEKGRADLEVEKLLYQINPHFLMNTLDTVHWLAVMN 344 + +M ++ ELI++ +++E + DLE+ L QINPHFL NTL+++ W A+MN Sbjct: 303 IGVISDNFDSMLKRVNELIEKTKLQEVEKRDLEIRMLQAQINPHFLFNTLNSLKWTALMN 362 Query: 345 GQKDIDKLVVSLNKLLYYNLGKRGLVSTIREEIDSIRQYLTLQQVRYDFGFDVRIHVDDD 404 + + + SL +LL + + + TIREE+++I+ Y+ +Q++RY FDV+ VD+ Sbjct: 363 QDYTVSEGLSSLAELLRNTIIDKNELVTIREELENIKNYVVIQKIRYGNSFDVQYEVDEL 422 Query: 405 VLELPIPRFILQPVLENSLYHGLHDEG---YIQVEVGKEEGQIIISVYDNGAGISAEEIA 461 +LE + +FILQP++ENS+ HG+ + I +++ EE I+IS+ DNG G +++E Sbjct: 423 LLENKVIKFILQPIVENSIIHGIDENKENQKIIIKIMSEEENILISIKDNGKGFNSKE-- 480 Query: 462 KLLHQQQVEQQKAGMGIGMSYVKRMIENQYGGLASLEIHSELGQGTTVFLKLP 514 +V+ K GIG + V I+ +G I+SE+ +GT V + +P Sbjct: 481 ------KVKDNKLS-GIGWNNVDERIKLTFGLGYGTTIYSEVNKGTIVKIVIP 526 >ref|NP_607635.1| putative histidine kinase [Streptococcus pyogenes MGAS8232] gb|AAL98134.1| putative histidine kinase [Streptococcus pyogenes MGAS8232] Length = 577 Score = 135 bits (339), Expect = 3e-30, Method: Compositional matrix adjust. Identities = 102/363 (28%), Positives = 170/363 (46%), Gaps = 15/363 (4%) Query: 163 KRIAYSDVPEDFAMDDYFADSGSSGDAGLQNGYYWFESTSNQGWSVISLIPVNEYNQQKN 222 K+ YS E AM Y A +G A ++ + + N W++I + +++ ++ + Sbjct: 221 KKSVYSSSKEMAAMKPYLAIK--NGYAKDKSSFVYQRVIPNSQWTLIGVASLDQLHRVQR 278 Query: 223 RWVIQMIYXXXXXXXXXXXIAWFLWKMVYVPLQQFNHQIKSIAQRNTAVTEVTRIXXXXX 282 + + + + P+QQ I +I + N + R Sbjct: 279 QIFWSFLGMGLLACLICGLTTILVLRKWIRPIQQLQQVILAIQKGNRQL----RAQETGS 334 Query: 283 PEFDFLLKQLQNMKNQIAELIQEVEVKEKGRADLEVEKLLYQINPHFLMNTLDTVHWLAV 342 PE L Q + +QI L+ V KEK ++ L QINPHFL NTLDT+ W+A Sbjct: 335 PELTDLAHQFNALLDQIDSLMVAVADKEKEIGQYRLQALASQINPHFLYNTLDTIIWMAE 394 Query: 343 MNGQKDIDKLVVSLNKLLYYNLGKRGLVSTIREEIDSIRQYLTLQQVRYDFGFDVRIHVD 402 N K + ++ SL K L + + +E+D + QYL +Q+ RY + Sbjct: 395 FNDSKRVVEVTKSLAKYFRLALNQGNDYIRLADELDHVSQYLFIQKQRYGDKLTYDVQGL 454 Query: 403 DDVLELPIPRFILQPVLENSLYHGLHD---EGYIQVEVGKEEGQIIISVYDNGAGISAEE 459 D + IP+ ILQP++EN++YHG+ + +G I+V V + +I++V+DNG GI A Sbjct: 455 DAYADFIIPKLILQPLVENAIYHGIKEVDRKGMIKVTVSETAQHLILTVWDNGKGIEA-- 512 Query: 460 IAKLLHQQQVEQQKAGMGIGMSYVKRMIENQYGGLASLEIHSELGQGTTVFLKLPSMEVL 519 + L + Q + + G+G+ V + ++ QYG + IHS+ T + L LP M L Sbjct: 513 -SALTNSQSLLTRG---GVGLKNVDQRLKLQYGEAYQMTIHSQSDHFTEIQLSLPKMHEL 568 Query: 520 PND 522 D Sbjct: 569 MAD 571 >ref|NP_623881.1| predicted ATPase [Thermoanaerobacter tengcongensis] gb|AAM25485.1| predicted ATPase [Thermoanaerobacter tengcongensis] Length = 593 Score = 134 bits (337), Expect = 5e-30, Method: Compositional matrix adjust. Identities = 84/277 (30%), Positives = 147/277 (53%), Gaps = 7/277 (2%) Query: 242 IAWFLWKMVYVPLQQFNHQIKSIAQRNTAVTEVTRIXXXXXPEFDFLLKQLQNMKNQIAE 301 ++ + + + PL++ + +K + Q N + E E ++K+ M +I + Sbjct: 316 VSMYFSRALTHPLERLANSMKKVRQGN--LEEKVEFEYHSKDEIAIVIKEYNRMVERIND 373 Query: 302 LIQEVEVKEKGRADLEVEKLLYQINPHFLMNTLDTVHWLAVMNGQKDIDKLVVSLNKLLY 361 LI + E + + E++ L QINPHFL NTLDT+ W A +++ +++ SL++L Sbjct: 374 LINRILKLEINKREAELKALEAQINPHFLYNTLDTIFWKAEKAKDRELSEMIYSLSRLFR 433 Query: 362 YNLGKRGLVSTIREEIDSIRQYLTLQQVRYDFGFDVRIHVDDDVLELPIPRFILQPVLEN 421 L + +R E + I +YL LQ RY F + + D++L+ IP+ ILQP +EN Sbjct: 434 LTLNRGEEFIQVRGEKELIERYLFLQSKRYKNKFKYSVKISDEILDYYIPKLILQPFVEN 493 Query: 422 SLYHGLH---DEGYIQVEVGKEEGQ-IIISVYDNGAGISAEEIAKLLHQQQVEQQKAGMG 477 ++ HGL +E +++V +G++EG+ + + DNG G+S E + KL + E + +G Sbjct: 494 AIVHGLENNSEESFVEV-IGEKEGENLKFIIKDNGPGMSEETLEKLKRFIESENIEEKVG 552 Query: 478 IGMSYVKRMIENQYGGLASLEIHSELGQGTTVFLKLP 514 + V + YG L+I S G+GTT+ L LP Sbjct: 553 YAIRNVNERLRLYYGDKYELKIESRQGEGTTISLILP 589 >ref|NP_388576.1| similar to two-component sensor histidine kinase [YesN] [Bacillus subtilis] pir||D69796 two-component sensor histidine kinase homolog yesM - Bacillus subtilis emb|CAB12514.1| yesM [Bacillus subtilis subsp. subtilis str. 168] Length = 577 Score = 133 bits (335), Expect = 1e-29, Method: Compositional matrix adjust. Identities = 81/266 (30%), Positives = 136/266 (51%), Gaps = 13/266 (4%) Query: 253 PLQQFNHQIKSIAQRNTAVTEVTRIXXXXXPEFDFLLKQLQNMKNQIAELIQEVEVKEKG 312 P++Q +KS+ + E L + M +I EL++E K+ Sbjct: 317 PIEQLVTAMKSVQHSGIEAGVSLSLPEHTQDEAGMLNRHFTVMMKRINELMEENVEKQLI 376 Query: 313 RADLEVEKLLYQINPHFLMNTLDTVHWLAVMNGQKDIDKLVVSLNKLLYYNLGKRGLVST 372 + E++ L QINPHFL NTL++++WLA N QK I K+V SL LL ++ + + T Sbjct: 377 IKETELKALQAQINPHFLYNTLESINWLAKANQQKQISKMVESLGFLLRNSIHMKKDIVT 436 Query: 373 IREEIDSIRQYLTLQQVRYDFGFDVRIHVDDDVLELPIPRFILQPVLENSLYHGLH---D 429 I+EE D +R Y+T+Q+ R++ + +DD+V IP+ LQP+ EN++ + L Sbjct: 437 IQEEADIVRHYMTIQRFRFEERLKFTLDIDDEVKHCLIPKLTLQPLAENAIQYALEPFTR 496 Query: 430 EGYIQVEVGKEEGQIIISVYDNGAGISAEEIAKLLHQQQVEQQKAGMGIGMSYVKRMIEN 489 I+++ K +G + I+V DNG G+ ++ + G GIG+ ++ I Sbjct: 497 PCAIRIQAKKAKGCVCITVEDNGPGMDG----------RILESTGGRGIGLWNIRERISL 546 Query: 490 QYGGLASLEIHSELGQGTTVFLKLPS 515 +G L IHSE +GT + + +P Sbjct: 547 TFGEPYGLRIHSEHEKGTRIVITIPC 572 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.318 0.137 0.392 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 752,902,793 Number of extensions: 32558324 Number of successful extensions: 87679 Number of sequences better than 1.0e-05: 222 Number of HSP's gapped: 88107 Number of HSP's successfully gapped: 222 Length of query: 522 Length of database: 757,237,578 Length adjustment: 130 Effective length of query: 392 Effective length of database: 404,274,708 Effective search space: 158475685536 Effective search space used: 158475685536 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 128 (53.9 bits)