BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0586.0084.p (176 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_242340.1| BH1474~unknown [Bacillus halodurans] >gi|254898... 54 2e-06 ref|NP_243340.1| BH2474~unknown conserved protein in B. subtilis... 54 2e-06 >ref|NP_242340.1| BH1474~unknown [Bacillus halodurans] pir||B83834 hypothetical protein BH1474 [imported] - Bacillus halodurans (strain C-125) dbj|BAB05193.1| BH1474~unknown [Bacillus halodurans] Length = 745 Score = 53.5 bits (127), Expect = 2e-06, Method: Compositional matrix adjust. Identities = 43/141 (30%), Positives = 64/141 (45%), Gaps = 3/141 (2%) Query: 1 VLLQVLSSSEVPPAVKEAAGXXXXXXXXXXXXXNTDRTAPFAQVTMFLPLRGPDGQETAS 60 +LL +L S E + A D + M LP E Sbjct: 572 LLLHMLRSEEEGSRTFQLANQALNNVTGQQLLSKADHHTNMQTLFMNLPFLLEKQVENLQ 631 Query: 61 VHIQSRR-GRKGELDAANCRLWFDLDMKQLGQTLVDVQVVDRIVSLKLHNDTPWVLELLE 119 V +QSR G+K +D NC L+F ++ K+LG+ + VQVVDR +S+ L ND E +E Sbjct: 632 VFVQSRNEGQK--IDWENCSLFFLIETKKLGEIGILVQVVDRQLSVTLKNDLDDFPEKME 689 Query: 120 ERKEEIGAAVEAIGYQLLSLR 140 E+ +E IGYQ+ ++ Sbjct: 690 PLVEKTSERIEEIGYQVSGIK 710 >ref|NP_243340.1| BH2474~unknown conserved protein in B. subtilis [Bacillus halodurans] pir||B83959 hypothetical protein BH2474 [imported] - Bacillus halodurans (strain C-125) dbj|BAB06193.1| BH2474~unknown conserved protein in B. subtilis [Bacillus halodurans] Length = 575 Score = 53.5 bits (127), Expect = 2e-06, Method: Compositional matrix adjust. Identities = 24/78 (30%), Positives = 44/78 (56%) Query: 35 TDRTAPFAQVTMFLPLRGPDGQETASVHIQSRRGRKGELDAANCRLWFDLDMKQLGQTLV 94 ++ + P Q + LPL Q ++H + +R G+L +CR+ F L + L +T+V Sbjct: 441 SETSGPITQTAIQLPLMLGSFQTDLTIHWEGKRQADGQLSPDHCRILFYLSLAHLKETIV 500 Query: 95 DVQVVDRIVSLKLHNDTP 112 DVQ+ RI+++ + N+ P Sbjct: 501 DVQIQHRILAVTIFNEHP 518 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.317 0.135 0.390 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 196,383,230 Number of extensions: 6246083 Number of successful extensions: 14482 Number of sequences better than 1.0e-05: 2 Number of HSP's gapped: 14483 Number of HSP's successfully gapped: 2 Length of query: 176 Length of database: 757,237,578 Length adjustment: 117 Effective length of query: 59 Effective length of database: 439,570,995 Effective search space: 25934688705 Effective search space used: 25934688705 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 121 (51.2 bits)