BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0586.0072.p (309 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_243335.1| succinyl-CoA synthetase (alpha subunit) [Bacill... 395 e-109 ref|NP_389492.1| succinyl-CoA synthetase (alpha subunit) [Bacill... 375 e-102 ref|NP_645946.1| succinyl-CoA synthetase [Staphylococcus aureus ... 374 e-102 ref|NP_371770.1| succinyl-CoA synthetase [Staphylococcus aureus ... 373 e-102 ref|NP_657803.1| CoA_binding, CoA binding domain [Bacillus anthr... 372 e-102 >ref|NP_243335.1| succinyl-CoA synthetase (alpha subunit) [Bacillus halodurans] pir||E83958 succinyl-CoA synthetase (alpha subunit) sucD [imported] - Bacillus halodurans (strain C-125) dbj|BAB06188.1| succinyl-CoA synthetase (alpha subunit) [Bacillus halodurans] Length = 302 Score = 395 bits (1014), Expect = e-109, Method: Compositional matrix adjust. Identities = 203/310 (65%), Positives = 231/310 (74%), Gaps = 11/310 (3%) Query: 1 MSILVDKNTKVITQGITGSTGLFHTKGALDYGTQMXXXXXXXXXXXXXNITLENGSEVS- 59 MSIL++K+TKVI QGITG+TGLFHTK A++YGT + + G+EV Sbjct: 1 MSILINKDTKVIVQGITGATGLFHTKQAVEYGTNIVGGVTPG----------KGGTEVEG 50 Query: 60 LPVFDXXXXXXXXXXXXXXXIYVPPAFAADSIMEAVDAGLELVICITEGIPVLDMVKVSR 119 +PVFD IYVPPAFAAD+IMEAVDA L+L ICITEGIPV+DMVKV R Sbjct: 51 IPVFDTVSQAVEATGANASVIYVPPAFAADAIMEAVDAELDLAICITEGIPVMDMVKVKR 110 Query: 120 FMEGRSTVLIGPNCPGVITPGECKIGIMPGYIHMPGYVGVVSRSGTLTYEAVHQLTERGI 179 +MEG+ T L+GPNCPGVITP ECKIGIMPGYIH G+VGVVSRSGTLTYEAVHQL+ GI Sbjct: 111 YMEGKKTRLVGPNCPGVITPEECKIGIMPGYIHKKGHVGVVSRSGTLTYEAVHQLSTAGI 170 Query: 180 GQSSAVGIGGDPVKGSEFIDILKLFNEDPGTKAVXXXXXXXXXXXXXXXXWIKENMTKPV 239 GQS+AVGIGGDPV G+ FID+LKLFNEDP T AV WIK NMTKPV Sbjct: 171 GQSTAVGIGGDPVNGTNFIDVLKLFNEDPDTYAVIMIGEIGGTAEEEAAEWIKANMTKPV 230 Query: 240 VGFIGGVTAPPGKRMGHAGAIISGGKGTASEKIAVLESCGIKVAPTPAEMGTTLVKVLEE 299 VGFIGG TAPPGKRMGHAGAIISGGKGTA+EKI LESCG+KVA TP+ MG TL+ VL+E Sbjct: 231 VGFIGGQTAPPGKRMGHAGAIISGGKGTAAEKIKTLESCGVKVAETPSVMGETLISVLKE 290 Query: 300 RGILNAFTTH 309 +G+L TTH Sbjct: 291 QGLLEKCTTH 300 >ref|NP_389492.1| succinyl-CoA synthetase (alpha subunit) [Bacillus subtilis] sp|P80865|SUCD_BACSU Succinyl-CoA synthetase alpha chain (SCS-alpha) (Vegetative protein 239) (VEG239) pir||F69719 succinate-CoA ligase (ADP-forming) (EC 6.2.1.5) alpha chain - Bacillus subtilis emb|CAA04420.1| putative succinyl-coA synthetase alpha chain [Bacillus subtilis] emb|CAB13483.1| succinyl-CoA synthetase (alpha subunit) [Bacillus subtilis subsp. subtilis str. 168] Length = 300 Score = 375 bits (962), Expect = e-102, Method: Compositional matrix adjust. Identities = 193/310 (62%), Positives = 221/310 (71%), Gaps = 11/310 (3%) Query: 1 MSILVDKNTKVITQGITGSTGLFHTKGALDYGTQMXXXXXXXXXXXXXNITLENGSEVS- 59 MS+ ++K+T+VI QGITGST LFHTK L+YGT + + G+E Sbjct: 1 MSVFINKDTRVIVQGITGSTALFHTKQMLEYGTNIVGGVTPG----------KGGTEAEG 50 Query: 60 LPVFDXXXXXXXXXXXXXXXIYVPPAFAADSIMEAVDAGLELVICITEGIPVLDMVKVSR 119 +PVF+ IYVP FAAD+IMEAVDA L+LVICITE IPVLDMVKV R Sbjct: 51 VPVFNTVAEAVQTTGANASVIYVPAPFAADAIMEAVDAELDLVICITEHIPVLDMVKVKR 110 Query: 120 FMEGRSTVLIGPNCPGVITPGECKIGIMPGYIHMPGYVGVVSRSGTLTYEAVHQLTERGI 179 FMEG+ T LIGPNCPGVITP ECKIGIMPGYIH G+VGVVSRSGTLTYEAVHQL+E G+ Sbjct: 111 FMEGKKTRLIGPNCPGVITPEECKIGIMPGYIHKKGHVGVVSRSGTLTYEAVHQLSEAGV 170 Query: 180 GQSSAVGIGGDPVKGSEFIDILKLFNEDPGTKAVXXXXXXXXXXXXXXXXWIKENMTKPV 239 GQS+AVGIGGDPV G+ FID+LK FNEDP T AV W+K NMTKPV Sbjct: 171 GQSTAVGIGGDPVNGTNFIDVLKAFNEDPDTHAVIMIGEIGGTAEEEAAEWVKANMTKPV 230 Query: 240 VGFIGGVTAPPGKRMGHAGAIISGGKGTASEKIAVLESCGIKVAPTPAEMGTTLVKVLEE 299 VGFIGG TAPPGKRMGHAGAIISGGKGTA EKI L +CGI+VA TP+ MG TL+KVL+E Sbjct: 231 VGFIGGKTAPPGKRMGHAGAIISGGKGTADEKIKTLNACGIEVAETPSVMGETLIKVLKE 290 Query: 300 RGILNAFTTH 309 + + TH Sbjct: 291 KNLFETCKTH 300 >ref|NP_645946.1| succinyl-CoA synthetase [Staphylococcus aureus subsp. aureus MW2] sp|Q8NX01|SUCD_STAAW Succinyl-CoA synthetase alpha chain (SCS-alpha) dbj|BAB94994.1| succinyl-CoA synthetase [Staphylococcus aureus subsp. aureus MW2] Length = 302 Score = 374 bits (961), Expect = e-102, Method: Compositional matrix adjust. Identities = 192/310 (61%), Positives = 220/310 (70%), Gaps = 13/310 (4%) Query: 1 MSILVDKNTKVITQGITGSTGLFHTKGALDYGTQMXXXXXXXXXXXXXNITLENGSEV-- 58 MS+ +DKNTKV+ QGITGST LFHTK LDYGT++ +T G +V Sbjct: 1 MSVFIDKNTKVMVQGITGSTALFHTKQMLDYGTKIVA-----------GVTPGKGGQVVE 49 Query: 59 SLPVFDXXXXXXXXXXXXXXXIYVPPAFAADSIMEAVDAGLELVICITEGIPVLDMVKVS 118 +PVF+ IYVP FAADSI+EA DA L++VICITE IPVLDMVKV Sbjct: 50 GVPVFNTVEEAKNETGATVSVIYVPAPFAADSILEAADADLDMVICITEHIPVLDMVKVK 109 Query: 119 RFMEGRSTVLIGPNCPGVITPGECKIGIMPGYIHMPGYVGVVSRSGTLTYEAVHQLTERG 178 R+++GR T L+GPNCPGVIT ECKIGIMPGYIH G+VGVVSRSGTLTYEAVHQLTE G Sbjct: 110 RYLQGRKTRLVGPNCPGVITADECKIGIMPGYIHKKGHVGVVSRSGTLTYEAVHQLTEEG 169 Query: 179 IGQSSAVGIGGDPVKGSEFIDILKLFNEDPGTKAVXXXXXXXXXXXXXXXXWIKENMTKP 238 IGQ++AVGIGGDPV G+ FID+LK FNED TKAV WIK NMTKP Sbjct: 170 IGQTTAVGIGGDPVNGTNFIDVLKAFNEDDETKAVVMIGEIGGTAEEEAAEWIKANMTKP 229 Query: 239 VVGFIGGVTAPPGKRMGHAGAIISGGKGTASEKIAVLESCGIKVAPTPAEMGTTLVKVLE 298 VVGFIGG TAPPGKRMGHAGAIISGGKGTA EKI L SCG+K A TP+E+G+TL++ + Sbjct: 230 VVGFIGGQTAPPGKRMGHAGAIISGGKGTAEEKIKTLNSCGVKTAATPSEIGSTLIEAAK 289 Query: 299 ERGILNAFTT 308 E GI A T Sbjct: 290 EAGIYEALLT 299 >ref|NP_371770.1| succinyl-CoA synthetase [Staphylococcus aureus subsp. aureus Mu50] ref|NP_374362.1| succinyl-CoA synthetase [Staphylococcus aureus subsp. aureus N315] sp|Q99UM4|SUCD_STAAM Succinyl-CoA synthetase alpha chain (SCS-alpha) pir||A89898 succinyl-CoA synthetase [imported] - Staphylococcus aureus (strain N315) dbj|BAB42341.1| succinyl-CoA synthetase [Staphylococcus aureus subsp. aureus N315] dbj|BAB57408.1| succinyl-CoA synthetase [Staphylococcus aureus subsp. aureus Mu50] Length = 302 Score = 373 bits (958), Expect = e-102, Method: Compositional matrix adjust. Identities = 191/310 (61%), Positives = 220/310 (70%), Gaps = 13/310 (4%) Query: 1 MSILVDKNTKVITQGITGSTGLFHTKGALDYGTQMXXXXXXXXXXXXXNITLENGSEV-- 58 MS+ +DKNTKV+ QGITGST LFHTK LDYGT++ +T G +V Sbjct: 1 MSVFIDKNTKVMVQGITGSTALFHTKQMLDYGTKIVA-----------GVTPGKGGQVVE 49 Query: 59 SLPVFDXXXXXXXXXXXXXXXIYVPPAFAADSIMEAVDAGLELVICITEGIPVLDMVKVS 118 +PVF+ IYVP FAADSI+EA DA L++VICITE IPVLDMVKV Sbjct: 50 GVPVFNTVEEAKNETGATVSVIYVPAPFAADSILEAADADLDMVICITEHIPVLDMVKVK 109 Query: 119 RFMEGRSTVLIGPNCPGVITPGECKIGIMPGYIHMPGYVGVVSRSGTLTYEAVHQLTERG 178 R+++GR T L+GPNCPGVIT ECKIGIMPGYIH G+VGVVSRSGTLTYEAVHQLTE G Sbjct: 110 RYLQGRKTRLVGPNCPGVITADECKIGIMPGYIHKKGHVGVVSRSGTLTYEAVHQLTEEG 169 Query: 179 IGQSSAVGIGGDPVKGSEFIDILKLFNEDPGTKAVXXXXXXXXXXXXXXXXWIKENMTKP 238 IGQ++AVGIGGDPV G+ FID+LK FNED TKAV WIK NMTKP Sbjct: 170 IGQTTAVGIGGDPVNGTNFIDVLKAFNEDDETKAVVMIGEIGGTAEEEAAEWIKANMTKP 229 Query: 239 VVGFIGGVTAPPGKRMGHAGAIISGGKGTASEKIAVLESCGIKVAPTPAEMGTTLVKVLE 298 VVGFIGG TAPPGKRMGHAGAIISGGKGTA EKI L SCG+K A TP+E+G+TL++ + Sbjct: 230 VVGFIGGQTAPPGKRMGHAGAIISGGKGTAEEKIKTLNSCGVKTAATPSEIGSTLIEAAK 289 Query: 299 ERGILNAFTT 308 E GI + T Sbjct: 290 EAGIYESLLT 299 >ref|NP_657803.1| CoA_binding, CoA binding domain [Bacillus anthracis A2012] ref|NP_846215.1| succinyl-CoA synthase, alpha subunit [Bacillus anthracis str. Ames] ref|NP_980174.1| succinyl-CoA synthase, alpha subunit [Bacillus cereus ATCC 10987] gb|AAP27701.1| succinyl-CoA synthase, alpha subunit [Bacillus anthracis str. Ames] gb|AAS42782.1| succinyl-CoA synthase, alpha subunit [Bacillus cereus ATCC 10987] Length = 300 Score = 372 bits (955), Expect = e-102, Method: Compositional matrix adjust. Identities = 189/305 (61%), Positives = 223/305 (73%), Gaps = 11/305 (3%) Query: 1 MSILVDKNTKVITQGITGSTGLFHTKGALDYGTQMXXXXXXXXXXXXXNITLENGSEVS- 59 MS+LV+K+TKVI QGITGS GLFHTK ++YGT++ + G+++ Sbjct: 1 MSVLVNKDTKVIVQGITGSQGLFHTKQMIEYGTKIVGGVTPG----------KGGTDIEG 50 Query: 60 LPVFDXXXXXXXXXXXXXXXIYVPPAFAADSIMEAVDAGLELVICITEGIPVLDMVKVSR 119 +PVFD +YVPPAFAAD+IMEAVDA ++LV+CITEGIPVLDMV V R Sbjct: 51 VPVFDTVEDAVKATGANASVVYVPPAFAADAIMEAVDAEIDLVVCITEGIPVLDMVNVKR 110 Query: 120 FMEGRSTVLIGPNCPGVITPGECKIGIMPGYIHMPGYVGVVSRSGTLTYEAVHQLTERGI 179 +M G+ T L+GPNCPGVITP ECKIGIMPGYIH G+VG+VSRSGTLTYEAVHQLT+ GI Sbjct: 111 YMAGKHTRLLGPNCPGVITPDECKIGIMPGYIHKKGHVGIVSRSGTLTYEAVHQLTQEGI 170 Query: 180 GQSSAVGIGGDPVKGSEFIDILKLFNEDPGTKAVXXXXXXXXXXXXXXXXWIKENMTKPV 239 GQS+AVGIGGDPV G++FID LK FNED T AV W+K NMTKPV Sbjct: 171 GQSTAVGIGGDPVNGTDFIDALKAFNEDEETHAVIMIGEIGGTAEEEAAEWVKANMTKPV 230 Query: 240 VGFIGGVTAPPGKRMGHAGAIISGGKGTASEKIAVLESCGIKVAPTPAEMGTTLVKVLEE 299 VGFIGG TAP GKRMGHAGAIISGGKGTA+EKI +E+CGIKVA TPA MG TL+ VL+E Sbjct: 231 VGFIGGQTAPAGKRMGHAGAIISGGKGTAAEKIKTMEACGIKVAETPAVMGETLISVLKE 290 Query: 300 RGILN 304 +G+ Sbjct: 291 KGLFE 295 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.317 0.138 0.404 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 395,140,538 Number of extensions: 14695623 Number of successful extensions: 38256 Number of sequences better than 1.0e-05: 919 Number of HSP's gapped: 36827 Number of HSP's successfully gapped: 920 Length of query: 309 Length of database: 757,237,578 Length adjustment: 125 Effective length of query: 184 Effective length of database: 417,850,203 Effective search space: 76884437352 Effective search space used: 76884437352 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 126 (53.1 bits)