BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0586.0066.p (141 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_00098550.1| COG1815: Flagellar basal body protein [Desulf... 76 3e-13 ref|NP_623063.1| Flagellar basal body protein [Thermoanaerobacte... 74 1e-12 gb|EAI48155.1| unknown [environmental sequence] 68 6e-11 ref|ZP_00181361.1| hypothetical protein Mther061801 [Moorella th... 68 8e-11 ref|NP_389500.1| flagellar basal-body rod protein [Bacillus subt... 63 3e-09 >ref|ZP_00098550.1| COG1815: Flagellar basal body protein [Desulfitobacterium hafniense] Length = 133 Score = 75.9 bits (185), Expect = 3e-13, Method: Compositional matrix adjust. Identities = 48/129 (37%), Positives = 69/129 (53%), Gaps = 3/129 (2%) Query: 10 LNSVSFQRLQGGLDAATKRQSVLANNVANADTPNFKRSDVNFESYLQQQENGLKSTLNAK 69 LN+ L+ LDA++ R VLANNVAN DTP+FKRSDV+F+ L + L K Sbjct: 5 LNTPVINILEKSLDASSLRYKVLANNVANVDTPDFKRSDVDFDLLLGEAMGQTGGELPLK 64 Query: 70 VTDSRHFQFGTGTIVPTATVSTDETTSXXXXXXXXXXXREQALSAENQLRYNSYVAQLNS 129 VT RH Q T ++ V D+ T+ +E AEN + YNS +++ Sbjct: 65 VTSERHLQ---KTGFNSSGVVQDQGTTYRNDGNNVDIDKEMVNVAENGIYYNSVTRAISA 121 Query: 130 QITMMRTVV 138 Q++ +RTV+ Sbjct: 122 QLSHLRTVI 130 >ref|NP_623063.1| Flagellar basal body protein [Thermoanaerobacter tengcongensis] gb|AAM24667.1| Flagellar basal body protein [Thermoanaerobacter tengcongensis] Length = 131 Score = 73.9 bits (180), Expect = 1e-12, Method: Compositional matrix adjust. Identities = 49/129 (37%), Positives = 69/129 (53%), Gaps = 10/129 (7%) Query: 18 LQGGLDAATKRQSVLANNVANADTPNFKRSDVNFESYLQQQENGLKSTLNAKVTDSRHFQ 77 L GL AAT R +V+ANN+AN DTPNFKRS+V FE LQ+ NG K L +T RH Sbjct: 8 LAKGLYAATVRSNVIANNIANVDTPNFKRSEVKFEEILQEALNGNK--LKGYITHPRHIP 65 Query: 78 FGTGTIVPTATVSTD----ETTSXXXXXXXXXXXREQALSAENQLRYNSYVAQLNSQI-T 132 G P ++ + TTS E + A+NQL Y + V +++ ++ + Sbjct: 66 IGPP---PIDSIKPEIYQVNTTSMRLDGNNVDIDYEMSQLAKNQLYYYALVQRVSGELNS 122 Query: 133 MMRTVVEGR 141 +M V +GR Sbjct: 123 IMTAVKDGR 131 >gb|EAI48155.1| unknown [environmental sequence] Length = 135 Score = 68.2 bits (165), Expect = 6e-11, Method: Compositional matrix adjust. Identities = 52/142 (36%), Positives = 68/142 (47%), Gaps = 16/142 (11%) Query: 9 LLNSVSFQRLQGGLDAATKRQSVLANNVANADTPNFKRSDVNFESYLQQ----QENGLKS 64 + NS F L LD T+RQ +L +N+AN DTP +K D+ FE L++ +E GL + Sbjct: 1 MFNSKIFTLLHKTLDFRTQRQDLLTSNIANKDTPGYKAEDMVFEKSLEKALHAEEPGLLN 60 Query: 65 TLNAKVTDSRHFQFGTGTIVPTATVSTDETTSXXXXXX----XXXXXREQALSAENQLRY 120 T TD RHF G T P V S RE A AENQL Y Sbjct: 61 T-----TDPRHFN-GRNT-PPLEMVKGQRIHSASPYPDFDGNTVDLDREMAKMAENQLMY 113 Query: 121 NSYVAQLNSQITMMRTVV-EGR 141 N+ + L Q M++T + EGR Sbjct: 114 NASIRMLTHQFRMLKTAITEGR 135 >ref|ZP_00181361.1| hypothetical protein Mther061801 [Moorella thermoacetica ATCC 39073] Length = 132 Score = 67.8 bits (164), Expect = 8e-11, Method: Compositional matrix adjust. Identities = 46/125 (36%), Positives = 65/125 (52%), Gaps = 4/125 (3%) Query: 18 LQGGLDAATKRQSVLANNVANADTPNFKRSDVNFESYLQQQENGLKSTLNAKVTDSRHFQ 77 L+ LD AT RQ +A+N+AN +TP FKR V+FE L + GL+ L T+ H Sbjct: 10 LEKALDTATLRQRTIAHNIANVNTPGFKRYQVSFEDQLSRAL-GLEPGLPLYRTNPYHLP 68 Query: 78 FGTGTIVPTATVSTDETTSXXXXXXXXXXXREQALSAENQLRYNSYVAQLNSQITMMRTV 137 + P VS D +T+ RE A+N L +N V QLN+++ M+R V Sbjct: 69 PSGLELAP--QVSRDTSTTMRQDGNNVDIDREMVDLAQNSLNFNVAVQQLNTRLAMLRYV 126 Query: 138 V-EGR 141 + EGR Sbjct: 127 INEGR 131 >ref|NP_389500.1| flagellar basal-body rod protein [Bacillus subtilis] sp|P24500|FLGB_BACSU Flagellar basal-body rod protein flgB pir||JG0019 flagellar basal-body rod protein flgB - Bacillus subtilis gb|AAA22441.1| flgB emb|CAB13491.1| flagellar basal-body rod protein [Bacillus subtilis subsp. subtilis str. 168] Length = 129 Score = 62.8 bits (151), Expect = 3e-09, Method: Compositional matrix adjust. Identities = 41/127 (32%), Positives = 62/127 (48%), Gaps = 6/127 (4%) Query: 14 SFQRLQGGLDAATKRQSVLANNVANADTPNFKRSDVNFESYLQQQENGLKSTLNAKVTDS 73 + Q L+ L A +Q V+ NN+AN DTPN+K V+F++ L Q+ S L A TD Sbjct: 7 TIQNLENALSRADIKQKVITNNIANIDTPNYKAKKVSFQNLLDQES----SRLEAIKTDY 62 Query: 74 RHFQFGTGTIVPTATVSTDETTSXXXXXXXXXXXREQALSAENQLRYNSYVAQLNSQITM 133 RH F + S D TS +E A+NQ+ Y + V ++N + Sbjct: 63 RHVDFSDTDSNYSIVASGD--TSYQQNGNNVDVDKEMTELAQNQINYQALVERMNGKFNS 120 Query: 134 MRTVVEG 140 ++TV+ G Sbjct: 121 LKTVLTG 127 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.311 0.125 0.335 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 163,932,888 Number of extensions: 5182812 Number of successful extensions: 9968 Number of sequences better than 1.0e-05: 36 Number of HSP's gapped: 9957 Number of HSP's successfully gapped: 36 Length of query: 141 Length of database: 757,237,578 Length adjustment: 102 Effective length of query: 39 Effective length of database: 480,297,480 Effective search space: 18731601720 Effective search space used: 18731601720 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 120 (50.8 bits)