BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0586.0061.p (290 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_00181356.1| hypothetical protein Mther061301 [Moorella th... 76 1e-12 ref|NP_243322.1| flagellar assembly protein [Bacillus halodurans... 66 1e-09 ref|NP_712770.1| flagellar biosynthesis/type III secretory pathw... 65 3e-09 ref|ZP_00080833.1| COG1317: Flagellar biosynthesis/type III secr... 64 6e-09 ref|NP_692478.1| flagellar assembly protein [Oceanobacillus ihey... 61 2e-08 >ref|ZP_00181356.1| hypothetical protein Mther061301 [Moorella thermoacetica ATCC 39073] Length = 205 Score = 75.9 bits (185), Expect = 1e-12, Method: Compositional matrix adjust. Identities = 45/138 (32%), Positives = 77/138 (55%) Query: 134 MSRKMAEMMEEARQVLQEAYRAKEVIIQEAEPFLVELSCSIAEKIVDKQLTVEPQFAMDL 193 + R+ A + EEAR+VL+EA +A I AE +++L+ +IA +++ +++ P+ + + Sbjct: 60 IRREAAAIREEARRVLEEARQAYRETITAAEGEILDLALTIATRVIGQEVEQRPEVVLGI 119 Query: 194 IRKNLARKREQGLISLCVSPAQFSFVNAAREELAMAVDSQAELQILPDSTVKDHGCVIRS 253 R+ + + E + SP + + REEL +A LQ++ D K GC I + Sbjct: 120 ARQAIRQVAEGQHYIIYASPEAAAIIRQHREELLAEAAPEARLQVVADPGFKTGGCRIET 179 Query: 254 SFGSIDARIDTQLAEIKK 271 G IDA +DTQL E+KK Sbjct: 180 ENGFIDASVDTQLEEVKK 197 >ref|NP_243322.1| flagellar assembly protein [Bacillus halodurans] pir||H83956 flagellar assembly protein fliH [imported] - Bacillus halodurans (strain C-125) dbj|BAB06175.1| flagellar assembly protein [Bacillus halodurans] Length = 266 Score = 65.9 bits (159), Expect = 1e-09, Method: Compositional matrix adjust. Identities = 35/123 (28%), Positives = 67/123 (54%) Query: 142 MEEARQVLQEAYRAKEVIIQEAEPFLVELSCSIAEKIVDKQLTVEPQFAMDLIRKNLARK 201 ++EA +++ A I+EA+P +++L+ ++ EKIV K+L + L+ + + Sbjct: 124 IKEAYAIVESAKDDYHRTIEEAQPTIIKLAAALCEKIVAKRLEEDESLWQSLVLQVMEEV 183 Query: 202 REQGLISLCVSPAQFSFVNAAREELAMAVDSQAELQILPDSTVKDHGCVIRSSFGSIDAR 261 RE +S+ V P + + ELA + + + + PD + D+GCV+ + FG IDA Sbjct: 184 REFEEVSIYVHPKWYEQTLRQKNELASILSGEGLIHVYPDLNLTDNGCVLETKFGRIDAS 243 Query: 262 IDT 264 +D+ Sbjct: 244 MDS 246 >ref|NP_712770.1| flagellar biosynthesis/type III secretory pathway protein [Leptospira interrogans serovar lai str. 56601] gb|AAN49788.1| flagellar biosynthesis/type III secretory pathway protein [Leptospira interrogans serovar lai str. 56601] gb|AAS69994.1| flagellar assembly protein [Leptospira interrogans serovar Copenhageni str. Fiocruz L1-130] Length = 307 Score = 64.7 bits (156), Expect = 3e-09, Method: Compositional matrix adjust. Identities = 38/122 (31%), Positives = 68/122 (55%) Query: 148 VLQEAYRAKEVIIQEAEPFLVELSCSIAEKIVDKQLTVEPQFAMDLIRKNLARKREQGLI 207 ++ +A +E IIQ +E +VE+ IA K++ ++ + ++ IR+ L R +++ + Sbjct: 176 IVGKAIDIREEIIQASEKQMVEMILIIARKVIKDEIIERKEIVLNNIREALKRIKDRDRV 235 Query: 208 SLCVSPAQFSFVNAAREELAMAVDSQAELQILPDSTVKDHGCVIRSSFGSIDARIDTQLA 267 + V+ A A ++EL ++S ++ I DS V G +I + G+IDARI TQL Sbjct: 236 DIRVNFADLELTTAHKDELIKLMESLRKVNIYEDSRVDRGGVIIETDVGAIDARISTQLK 295 Query: 268 EI 269 EI Sbjct: 296 EI 297 >ref|ZP_00080833.1| COG1317: Flagellar biosynthesis/type III secretory pathway protein [Geobacter metallireducens] Length = 304 Score = 63.5 bits (153), Expect = 6e-09, Method: Compositional matrix adjust. Identities = 33/139 (23%), Positives = 74/139 (53%) Query: 136 RKMAEMMEEARQVLQEAYRAKEVIIQEAEPFLVELSCSIAEKIVDKQLTVEPQFAMDLIR 195 R +A + + R + + +++E+E +++L+ +A KIV +++T +P +I Sbjct: 113 RGLANVFKSLRDGVSAVTGLRSRLLKESEEDVLKLAVMVARKIVQQEITQDPAILTSIIA 172 Query: 196 KNLARKREQGLISLCVSPAQFSFVNAAREELAMAVDSQAELQILPDSTVKDHGCVIRSSF 255 + E+ + + ++P ++ V A R+ + + + PD V GC++ ++ Sbjct: 173 AAVGGCTERDRVVVRLNPNDYTVVAANRQAFLAGLGDDVPITLTPDDGVGPGGCLVETAT 232 Query: 256 GSIDARIDTQLAEIKKELI 274 G++DARI++QL EI + L+ Sbjct: 233 GTVDARIESQLDEIYRTLL 251 >ref|NP_692478.1| flagellar assembly protein [Oceanobacillus iheyensis HTE831] dbj|BAC13513.1| flagellar assembly protein [Oceanobacillus iheyensis HTE831] Length = 245 Score = 61.2 bits (147), Expect = 2e-08, Method: Compositional matrix adjust. Identities = 30/108 (27%), Positives = 62/108 (57%) Query: 167 LVELSCSIAEKIVDKQLTVEPQFAMDLIRKNLARKREQGLISLCVSPAQFSFVNAAREEL 226 ++ LS +AE+I++ QLT P +++++ + + Q +S+ + P + V+ + EL Sbjct: 130 ILGLSVLVAERILENQLTEHPDQFLNIVKAAVHDIKGQPKVSIILHPTNYELVSTHQTEL 189 Query: 227 AMAVDSQAELQILPDSTVKDHGCVIRSSFGSIDARIDTQLAEIKKELI 274 + ++L I + +K+ C+I FG I+A ID+QL +I++ L+ Sbjct: 190 KRMLGKDSKLAIYVNEDLKETDCLIEHPFGQIEANIDSQLTKIREALM 237 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.313 0.127 0.326 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 299,311,552 Number of extensions: 9741034 Number of successful extensions: 31093 Number of sequences better than 1.0e-05: 18 Number of HSP's gapped: 31097 Number of HSP's successfully gapped: 18 Length of query: 290 Length of database: 757,237,578 Length adjustment: 124 Effective length of query: 166 Effective length of database: 420,565,302 Effective search space: 69813840132 Effective search space used: 69813840132 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 125 (52.8 bits)