BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0586.0043.p (364 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value emb|CAA52218.1| flagellar biosynthetic protein [Bacillus subtilis] 328 1e-88 ref|NP_389520.1| flagella-associated protein [Bacillus subtilis]... 328 1e-88 ref|NP_243305.1| flagella-associated protein [Bacillus haloduran... 317 4e-85 ref|NP_692495.1| flagella-associated protein [Oceanobacillus ihe... 314 2e-84 ref|ZP_00183579.1| hypothetical protein Exigu101101 [Exiguobacte... 297 3e-79 >emb|CAA52218.1| flagellar biosynthetic protein [Bacillus subtilis] Length = 360 Score = 328 bits (841), Expect = 1e-88, Method: Compositional matrix adjust. Identities = 157/354 (44%), Positives = 232/354 (65%) Query: 9 LNLQHFGGDKTEKATPKKRQEARKKGQVAKXXXXXXXXXXXXXXXXXMMFGGFIKERVVK 68 ++LQ F G+KTEKATPKKR++ RKKGQVAK + G ++++R++ Sbjct: 5 VDLQFFAGEKTEKATPKKRKDTRKKGQVAKSSDVNSAVSLLVIFLSLIAIGPYMRDRLLS 64 Query: 69 LYTDVFQNRMMMQVTPENVSEMFRHYGLQILILLAPLFIITFVMALVVNLAEVGFMMVTE 128 + + M+++ NV +F + ++LAP+ ++ V +V N +VGF+ E Sbjct: 65 FIETFYTESLTMKLSESNVHTLFVSLLKDMGMILAPILLVALVAGVVSNYMQVGFLFSAE 124 Query: 129 GLTPKFSKINPIKGFKNIFSMRSFVEFLKSIFKLLIIGYLVYSTLWGEKESFASLAHVSA 188 + PK K++PIKGFK I+SMR+ VE +KSI K++++G+ ++ LW L ++ Sbjct: 125 VIQPKLEKLDPIKGFKRIYSMRAIVELIKSILKIVVVGFAAFAVLWLHYGEILRLPLLTP 184 Query: 189 EGAYRFAAKMTMNLGIKIGAVLLIMAVFDYIYQKYEHEKSLKMSKQDIKDEYKKMEGDPI 248 E A F +K+T+ +G+ LLI+A DY+YQ++++EK++KMSKQDIKDEYKK EGDPI Sbjct: 185 EEALSFVSKLTLWMGLSGAGALLILAGLDYLYQRFDYEKNIKMSKQDIKDEYKKSEGDPI 244 Query: 249 IKGKIREXXXXXXXXXXXXEVPNADVIITNPTHFAVALKYDGSQMEAPQIIAKGQDFVAL 308 IK KI++ EVP ADVIITNPTH+A+ALKYD +M+AP I+AKG D +AL Sbjct: 245 IKSKIKQRQREMAMRRMMQEVPKADVIITNPTHYAIALKYDEEKMDAPYIVAKGVDHLAL 304 Query: 309 RIRELAKEHGVITMENKPLARALFQRTEIGDVVPADLFQAVAEVLAYVYKLNGK 362 +IR++AKEH V+ +EN+PLARAL+ + EI VP + F+ +AE+LAYVYK K Sbjct: 305 KIRKIAKEHDVMMVENRPLARALYDQVEIDQAVPEEFFKVLAEILAYVYKTKQK 358 >ref|NP_389520.1| flagella-associated protein [Bacillus subtilis] sp|P35538|FLHB_BACSU Flagellar biosynthetic protein flhB pir||D69623 flagellar biosynthetic protein flhB - Bacillus subtilis emb|CAB13511.1| flagella-associated protein [Bacillus subtilis subsp. subtilis str. 168] Length = 360 Score = 328 bits (841), Expect = 1e-88, Method: Compositional matrix adjust. Identities = 157/354 (44%), Positives = 232/354 (65%) Query: 9 LNLQHFGGDKTEKATPKKRQEARKKGQVAKXXXXXXXXXXXXXXXXXMMFGGFIKERVVK 68 ++LQ F G+KTEKATPKKR++ RKKGQVAK + G ++++R++ Sbjct: 5 VDLQFFAGEKTEKATPKKRKDTRKKGQVAKSSDVNTAVSLLVIFLSLIAIGPYMRDRLLS 64 Query: 69 LYTDVFQNRMMMQVTPENVSEMFRHYGLQILILLAPLFIITFVMALVVNLAEVGFMMVTE 128 + + M+++ NV +F + ++LAP+ ++ V +V N +VGF+ E Sbjct: 65 FIETFYTESLTMKLSESNVHTLFVSLLKDMGMILAPILLVALVAGVVSNYMQVGFLFSAE 124 Query: 129 GLTPKFSKINPIKGFKNIFSMRSFVEFLKSIFKLLIIGYLVYSTLWGEKESFASLAHVSA 188 + PK K++PIKGFK I+SMR+ VE +KSI K++++G+ ++ LW L ++ Sbjct: 125 VIQPKLEKLDPIKGFKRIYSMRAIVELIKSILKIVVVGFAAFAVLWLHYGEILRLPLLTP 184 Query: 189 EGAYRFAAKMTMNLGIKIGAVLLIMAVFDYIYQKYEHEKSLKMSKQDIKDEYKKMEGDPI 248 E A F +K+T+ +G+ LLI+A DY+YQ++++EK++KMSKQDIKDEYKK EGDPI Sbjct: 185 EEALSFVSKLTLWMGLSGAGALLILAGLDYLYQRFDYEKNIKMSKQDIKDEYKKSEGDPI 244 Query: 249 IKGKIREXXXXXXXXXXXXEVPNADVIITNPTHFAVALKYDGSQMEAPQIIAKGQDFVAL 308 IK KI++ EVP ADVIITNPTH+A+ALKYD +M+AP I+AKG D +AL Sbjct: 245 IKSKIKQRQREMAMRRMMQEVPKADVIITNPTHYAIALKYDEEKMDAPYIVAKGVDHLAL 304 Query: 309 RIRELAKEHGVITMENKPLARALFQRTEIGDVVPADLFQAVAEVLAYVYKLNGK 362 +IR++AKEH V+ +EN+PLARAL+ + EI VP + F+ +AE+LAYVYK K Sbjct: 305 KIRKIAKEHDVMMVENRPLARALYDQVEIDQAVPEEFFKVLAEILAYVYKTKQK 358 >ref|NP_243305.1| flagella-associated protein [Bacillus halodurans] pir||G83954 flagella-associated protein flhB [imported] - Bacillus halodurans (strain C-125) dbj|BAB06158.1| flagella-associated protein [Bacillus halodurans] Length = 363 Score = 317 bits (811), Expect = 4e-85, Method: Compositional matrix adjust. Identities = 166/356 (46%), Positives = 238/356 (66%), Gaps = 1/356 (0%) Query: 9 LNLQHFGGDKTEKATPKKRQEARKKGQVAKXXXXXXXXXXXXXXXXXMMFGG-FIKERVV 67 ++LQ F +KTEKATPKKRQ++RKKGQVAK +FGG + + Sbjct: 7 IDLQFFAQEKTEKATPKKRQDSRKKGQVAKSTDVNTAIILLCVFLFLWLFGGPLLGGTLE 66 Query: 68 KLYTDVFQNRMMMQVTPENVSEMFRHYGLQILILLAPLFIITFVMALVVNLAEVGFMMVT 127 KL F++ ++M VT E+V+E+ ++ I+L P+ I + ++ + +VG + T Sbjct: 67 KLLIHTFESYLLMDVTEESVAELMFEITMEAAIVLLPIMAIALIAGVLASYIQVGPLFTT 126 Query: 128 EGLTPKFSKINPIKGFKNIFSMRSFVEFLKSIFKLLIIGYLVYSTLWGEKESFASLAHVS 187 E L K SKI+PIKG K IFS+R+ VEFLKS+ K+++ G +S +W E L+ S Sbjct: 127 EPLKMKLSKIDPIKGAKRIFSVRALVEFLKSLLKIVLTGLAAFSIVWFSLEDVMLLSQKS 186 Query: 188 AEGAYRFAAKMTMNLGIKIGAVLLIMAVFDYIYQKYEHEKSLKMSKQDIKDEYKKMEGDP 247 E ++ A++T LGI I +LLI+++ DY+YQ+Y+HEK ++MSKQD+KDE+K +EGDP Sbjct: 187 VENSFVTVAQLTALLGILIAILLLILSIPDYLYQRYDHEKQIRMSKQDVKDEHKNVEGDP 246 Query: 248 IIKGKIREXXXXXXXXXXXXEVPNADVIITNPTHFAVALKYDGSQMEAPQIIAKGQDFVA 307 +K K ++ EVP ADV+ITNPTH+A+ALKYD S+M+AP I+AKG D+VA Sbjct: 247 KVKSKRKQKQMEMAMQRMMQEVPKADVVITNPTHYAIALKYDQSKMDAPVIVAKGVDYVA 306 Query: 308 LRIRELAKEHGVITMENKPLARALFQRTEIGDVVPADLFQAVAEVLAYVYKLNGKR 363 +I+ LAK+H V+T+ENKPLARAL+ + +IGD VP LF+AVAEVLAYVY+L K+ Sbjct: 307 FKIKSLAKQHNVVTVENKPLARALYDQADIGDQVPEQLFKAVAEVLAYVYRLKKKQ 362 >ref|NP_692495.1| flagella-associated protein [Oceanobacillus iheyensis HTE831] dbj|BAC13530.1| flagella-associated protein [Oceanobacillus iheyensis HTE831] Length = 359 Score = 314 bits (805), Expect = 2e-84, Method: Compositional matrix adjust. Identities = 160/354 (45%), Positives = 224/354 (63%) Query: 9 LNLQHFGGDKTEKATPKKRQEARKKGQVAKXXXXXXXXXXXXXXXXXMMFGGFIKERVVK 68 L++Q F G+KTEKATPKKR + RKKG+VAK +FG ++ + + Sbjct: 5 LDIQFFAGEKTEKATPKKRLDERKKGRVAKSQDVNTALLLLGCFIALFVFGSYMLQYMTG 64 Query: 69 LYTDVFQNRMMMQVTPENVSEMFRHYGLQILILLAPLFIITFVMALVVNLAEVGFMMVTE 128 + F + +T +NV MF L ++LAP+ II + + NL +V F+ TE Sbjct: 65 FFEKSFTEYIHWDLTEKNVQLMFNSASLDAGMMLAPIMIIAIITGMAANLVQVRFLFSTE 124 Query: 129 GLTPKFSKINPIKGFKNIFSMRSFVEFLKSIFKLLIIGYLVYSTLWGEKESFASLAHVSA 188 L KI+PI+G K IFS+R+ VEFLKS+ K++ +G + + +W K+ A + A Sbjct: 125 PLKFDLKKIDPIQGAKRIFSIRALVEFLKSMLKIVFVGIVTFYVIWIFKDDMLMTAFLDA 184 Query: 189 EGAYRFAAKMTMNLGIKIGAVLLIMAVFDYIYQKYEHEKSLKMSKQDIKDEYKKMEGDPI 248 +GA F A +T +G +L+ +A+FDY YQK++ EK+++MSKQDIKDEYK +EGDP+ Sbjct: 185 KGALGFFATVTAIMGFAAIILLVFLALFDYWYQKFDFEKNIRMSKQDIKDEYKNVEGDPL 244 Query: 249 IKGKIREXXXXXXXXXXXXEVPNADVIITNPTHFAVALKYDGSQMEAPQIIAKGQDFVAL 308 IK KI+E EVPNADVIITNPTH+AVA+KYD S+ AP I+AKG D +A Sbjct: 245 IKSKIKEKQRQMATRRMMSEVPNADVIITNPTHYAVAIKYDESKANAPYILAKGTDQIAQ 304 Query: 309 RIRELAKEHGVITMENKPLARALFQRTEIGDVVPADLFQAVAEVLAYVYKLNGK 362 +I+E+AK H VI +EN+PLAR L EIG+++P FQAVAEVLAYVY++ K Sbjct: 305 KIKEIAKNHDVIMVENRPLARGLHDAVEIGEIIPEAYFQAVAEVLAYVYRMEKK 358 >ref|ZP_00183579.1| hypothetical protein Exigu101101 [Exiguobacterium sp. 255-15] Length = 363 Score = 297 bits (760), Expect = 3e-79, Method: Compositional matrix adjust. Identities = 148/352 (42%), Positives = 218/352 (61%), Gaps = 1/352 (0%) Query: 9 LNLQHFGGDKTEKATPKKRQEARKKGQVAKXXXXXXXXXXXXXXXXXMMFGGFIKERVVK 68 L+LQ+F G+KTEKA+P+KR ++RKKGQVAK FG F+ +R V Sbjct: 8 LDLQYFAGEKTEKASPRKRDDSRKKGQVAKSADLTSSLMLFGMFLILFFFGPFLGKRFVM 67 Query: 69 LYTDVFQNRMMMQVTPEN-VSEMFRHYGLQILILLAPLFIITFVMALVVNLAEVGFMMVT 127 + + + ++ E + E+ +Q+ IL+AP F+ V+ ++ N ++G ++ T Sbjct: 68 VLENGLSAQTVLVAVEEGRIQEVMIEMLVQMGILVAPFFVTAVVIGILGNFVQIGALLST 127 Query: 128 EGLTPKFSKINPIKGFKNIFSMRSFVEFLKSIFKLLIIGYLVYSTLWGEKESFASLAHVS 187 E + PK +INP++G K I SM++ +EFLKS+FKLL+IG + LW K + L Sbjct: 128 ESIQPKLDRINPLQGIKRIVSMKALIEFLKSVFKLLVIGATSGTVLWNNKVEISRLTSEP 187 Query: 188 AEGAYRFAAKMTMNLGIKIGAVLLIMAVFDYIYQKYEHEKSLKMSKQDIKDEYKKMEGDP 247 A +T LGI + L+++AV D+ YQK++ EKS++MSKQD+KDE+K EGDP Sbjct: 188 IGDALAIIGHLTFLLGISVSIALIVLAVLDFAYQKFDFEKSIRMSKQDLKDEHKNTEGDP 247 Query: 248 IIKGKIREXXXXXXXXXXXXEVPNADVIITNPTHFAVALKYDGSQMEAPQIIAKGQDFVA 307 IKGKI++ E+PNADV+ITNPTH+AVA++YD + AP ++AKG D VA Sbjct: 248 QIKGKIKQQQREMAMRRMMQEIPNADVVITNPTHYAVAIQYDDGKHAAPIVVAKGVDAVA 307 Query: 308 LRIRELAKEHGVITMENKPLARALFQRTEIGDVVPADLFQAVAEVLAYVYKL 359 RIRE A + +EN+PLARALF ++EIG V +QA+AE LA+VY++ Sbjct: 308 FRIREKATAAEIPIIENRPLARALFAKSEIGMAVDESFYQALAETLAFVYQM 359 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.323 0.138 0.389 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 444,483,132 Number of extensions: 15706815 Number of successful extensions: 52204 Number of sequences better than 1.0e-05: 270 Number of HSP's gapped: 51882 Number of HSP's successfully gapped: 270 Length of query: 364 Length of database: 757,237,578 Length adjustment: 127 Effective length of query: 237 Effective length of database: 412,420,005 Effective search space: 97743541185 Effective search space used: 97743541185 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 126 (53.1 bits)