BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0586.0040.p (479 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_243303.1| flagella-associated protein [Bacillus haloduran... 225 2e-57 ref|NP_348764.1| Flagellar GTP-binding protein, FlhF [Clostridiu... 198 3e-49 ref|NP_692497.1| flagella-associated protein [Oceanobacillus ihe... 187 5e-46 ref|NP_782259.1| flagellar biosynthesis protein flhF [Clostridiu... 186 2e-45 gb|EAJ97763.1| unknown [environmental sequence] 178 2e-43 >ref|NP_243303.1| flagella-associated protein [Bacillus halodurans] pir||E83954 flagella-associated protein flhF [imported] - Bacillus halodurans (strain C-125) dbj|BAB06156.1| flagella-associated protein [Bacillus halodurans] Length = 375 Score = 225 bits (573), Expect = 2e-57, Method: Compositional matrix adjust. Identities = 129/309 (41%), Positives = 188/309 (60%), Gaps = 8/309 (2%) Query: 174 EARNKKLNAASTSELESDVLREIRDMKQWMERIARYSSGAVELPEQLEQIRDRLMEQETD 233 + R +N ST L L EI+++K+ M+ I + PE ++L +QE Sbjct: 69 DGRPSSVNEKSTESL----LVEIQELKKMMQTINTKETSMQSYPEPFHSFDEQLKQQEVC 124 Query: 234 AVLVDEWISNIFDQW-HEEG---RIWTPEEFNVRMAAQVEDFLSGRIANGIAPDTRIVYI 289 + + + ++ W EEG W+ EE + ++ LS GI + V I Sbjct: 125 EAVRLQLVKHLLQAWLQEEGDEQTPWSYEELLTKGEQLLKRKLSLLPEGGITFQKKFVNI 184 Query: 290 AGPTGVGKTTTIAKLAAEQLFRQGRKVGLITSDTYRISAVEQLRTYAAILNMPLEVVQSP 349 GPTGVGKTTTIAKLAA + ++ +KV LIT+DTYRI+AVEQL+TYA ILN+PLEVV S Sbjct: 185 VGPTGVGKTTTIAKLAAHSVLKKKKKVALITTDTYRIAAVEQLKTYAKILNIPLEVVYSM 244 Query: 350 GDLQRALFRLESCDLVLMDTAGRNYRNEMLVAELQSLLAKELKSETFLVLSLTSKSRDMK 409 D Q+A + DLVL+D+AGRN+RN + V EL+ ++ + ++E +LVLSLT K RDMK Sbjct: 245 EDFQKAKNQFIHYDLVLVDSAGRNFRNALYVKELEKVIDFKHEAENYLVLSLTGKYRDMK 304 Query: 410 KIVEHFGRYQLDKVIFTKLDETGSYGPLFNVLNDFPLKLSYITNGQNVPDDLLAATKDQL 469 I+E F + ++IFTK DET +YG + N++ + ++Y+TNGQNVPDD++ ++ D + Sbjct: 305 AIIEQFSLVPIKQIIFTKQDETDTYGAMLNMMFEQGKGVAYVTNGQNVPDDIVESSNDWI 364 Query: 470 SGMILGTGG 478 ILG G Sbjct: 365 IQTILGAKG 373 >ref|NP_348764.1| Flagellar GTP-binding protein, FlhF [Clostridium acetobutylicum] pir||E97164 flagellar GTP-binding protein, FlhF [imported] - Clostridium acetobutylicum gb|AAK80104.1| Flagellar GTP-binding protein, FlhF [Clostridium acetobutylicum] Length = 389 Score = 198 bits (503), Expect = 3e-49, Method: Compositional matrix adjust. Identities = 109/287 (37%), Positives = 176/287 (61%), Gaps = 15/287 (5%) Query: 189 ESDVLREIRDMKQWMERIARYSSGAVELPEQLEQIRDRLMEQETDAVLVDEWISNIFDQW 248 E +LRE+ +MK+ + I+ SS A + E+ +I +L E + + + E + NI D Sbjct: 113 EDRILREMEEMKKLIGNIS--SSNADQ--EEKSEIELKLEEADINEGYIKEIVKNIMD-- 166 Query: 249 HEEGRIWTPEEFNVRMAAQVEDFLSGRIANGIAPDTRIVYIAGPTGVGKTTTIAKLAAEQ 308 ++ ++ + +++ L I + I+ GPTGVGKTTTIAKLA + Sbjct: 167 ---------DKSDIDLNLKLKSALEKNIEISKTEEKGIITFVGPTGVGKTTTIAKLAGKF 217 Query: 309 LFRQGRKVGLITSDTYRISAVEQLRTYAAILNMPLEVVQSPGDLQRALFRLESCDLVLMD 368 + +KVGLIT DTYRI AVEQLRTYA I+N+P +VV + ++ A+ +++CD++L+D Sbjct: 218 SLIEKKKVGLITVDTYRIGAVEQLRTYADIMNLPFKVVLTLKEMDEAIKSMQNCDIILVD 277 Query: 369 TAGRNYRNEMLVAELQSLLAKELKSETFLVLSLTSKSRDMKKIVEHFGRYQLDKVIFTKL 428 T GR+ +N+M ++EL++ ++K LV+S T+K+RD++ I++ + + VI TKL Sbjct: 278 TTGRSSKNKMQISELRAFVSKTNSLNINLVISATTKNRDLEAIIDGYRQLDFQNVIITKL 337 Query: 429 DETGSYGPLFNVLNDFPLKLSYITNGQNVPDDLLAATKDQLSGMILG 475 DET +YG + N+LN LSY+T GQNVPDD+ K ++ ++LG Sbjct: 338 DETTTYGSIINILNYAHKPLSYVTTGQNVPDDIRRMAKGEIVSLVLG 384 >ref|NP_692497.1| flagella-associated protein [Oceanobacillus iheyensis HTE831] dbj|BAC13532.1| flagella-associated protein [Oceanobacillus iheyensis HTE831] Length = 372 Score = 187 bits (475), Expect = 5e-46, Method: Compositional matrix adjust. Identities = 108/295 (36%), Positives = 173/295 (58%), Gaps = 4/295 (1%) Query: 181 NAASTSELESDVLREIRDMKQWMERIARYSSGAVELPEQLEQIRDRLMEQETDAVLVDEW 240 N ++ E+ + EI ++++ +E R S+ QL + L+ QE + ++ Sbjct: 77 NHNRKTKDENPLREEILNIQRLIEAQGRQSNHEFTPIYQLA--YEHLIAQEISPTIAEQL 134 Query: 241 ISNIFDQWHEEGRIWTPEEFNVRMAAQVEDFLSGRIANGIAPDTRIVYIAGPTGVGKTTT 300 I + D + E + + + + L ++ G D RI+ GPTGVGKTTT Sbjct: 135 IKEVRDDETNQDEYHNLESILINVKQLIVNRLE-QVDIGFTYDHRIIQFVGPTGVGKTTT 193 Query: 301 IAKLAAEQLFRQGRKVGLITSDTYRISAVEQLRTYAAILNMPLEVVQSPGDLQRALFRLE 360 IAK+AA+ + +KV IT DTYRI+AV+QL+TYA IL++P+EV S D ++A+ + Sbjct: 194 IAKIAAKLYLEEKKKVAFITLDTYRIAAVDQLKTYAKILHIPVEVAYSKEDYRKAVEKFS 253 Query: 361 SCDLVLMDTAGRNYRNEMLVAELQSLLAKELKSETFLVLSLTSKSRDMKKIVEHFGRYQL 420 S D++L+DTAGRNYR+ + E+Q LK +LVLS+TSK +DM I + F + Sbjct: 254 SFDVILVDTAGRNYRDNQYLNEIQE-WPSSLKVTNYLVLSMTSKPKDMLDIFQRFQLLGI 312 Query: 421 DKVIFTKLDETGSYGPLFNVLNDFPLKLSYITNGQNVPDDLLAATKDQLSGMILG 475 V+FTKLDET YG L N+ + +++ Y+TNGQ+VP+D++ + +++S +IL Sbjct: 313 HGVVFTKLDETEQYGNLLNISWNNKIRIGYMTNGQDVPEDVIQPSAEEISHLILS 367 >ref|NP_782259.1| flagellar biosynthesis protein flhF [Clostridium tetani E88] gb|AAO36196.1| flagellar biosynthesis protein flhF [Clostridium tetani E88] Length = 377 Score = 186 bits (471), Expect = 2e-45, Method: Compositional matrix adjust. Identities = 105/286 (36%), Positives = 167/286 (58%), Gaps = 13/286 (4%) Query: 192 VLREIRDMKQWMERIA--RYSSGAVELPEQLEQIRDRLMEQETDAVLVDEWISNIFDQWH 249 +L+EI +MK Y E +++++I+ L + + + ++ I I D+ + Sbjct: 98 ILKEIEEMKNAFTSFVDNTYKGNIEEERQEIKEIKSILKDND----ISEDIIEKILDELN 153 Query: 250 EEGRIWTPEEFNVRMAAQVEDFLSGRIANGIAPDTRIVYIAGPTGVGKTTTIAKLAAEQL 309 E I E + ++F+ R+ V + GPTGVGKTTTIAKLA Sbjct: 154 EVENITEALE-------KTKEFIEDRVRVSSEDLKGAVVLVGPTGVGKTTTIAKLAGRLS 206 Query: 310 FRQGRKVGLITSDTYRISAVEQLRTYAAILNMPLEVVQSPGDLQRALFRLESCDLVLMDT 369 + +KVGLIT DTYRI AVEQL+TYA I+ +P +VV S D++ A+ +++C+++L+DT Sbjct: 207 LVEKKKVGLITVDTYRIGAVEQLKTYADIMKIPFKVVFSIKDMEDAIEDMKNCEVILVDT 266 Query: 370 AGRNYRNEMLVAELQSLLAKELKSETFLVLSLTSKSRDMKKIVEHFGRYQLDKVIFTKLD 429 GR+ +N M ++EL++ + K LV+S T+K++D+ IVE + VI TKLD Sbjct: 267 TGRSSKNVMQISELRAFIEKVNSENIHLVISSTTKNKDIDSIVEGYKTLGYKSVIITKLD 326 Query: 430 ETGSYGPLFNVLNDFPLKLSYITNGQNVPDDLLAATKDQLSGMILG 475 ET +YG + N+ N +S+IT GQNVP+D+ T +++G+ILG Sbjct: 327 ETTTYGSILNIANSTKKPISFITTGQNVPEDIRLVTPKEITGLILG 372 >gb|EAJ97763.1| unknown [environmental sequence] Length = 217 Score = 178 bits (452), Expect = 2e-43, Method: Compositional matrix adjust. Identities = 92/207 (44%), Positives = 131/207 (63%), Gaps = 3/207 (1%) Query: 275 RIANGIA---PDTRIVYIAGPTGVGKTTTIAKLAAEQLFRQGRKVGLITSDTYRISAVEQ 331 + NGI P +IV + GPTGVGKTTT AK+ +EQ+ + RKV LIT DT RI+AVEQ Sbjct: 10 KTTNGIENIEPSQKIVSLIGPTGVGKTTTAAKICSEQILQYKRKVALITVDTMRIAAVEQ 69 Query: 332 LRTYAAILNMPLEVVQSPGDLQRALFRLESCDLVLMDTAGRNYRNEMLVAELQSLLAKEL 391 LR YA ++N+PL +V +L RA+ D++++DT G + R+E + EL+ + + Sbjct: 70 LRAYAKVINVPLSIVSDKVELDRAISSYADYDVIVIDTGGCSQRDETQMFELRDIFDERG 129 Query: 392 KSETFLVLSLTSKSRDMKKIVEHFGRYQLDKVIFTKLDETGSYGPLFNVLNDFPLKLSYI 451 + LVLS T+K D +I FG LD ++FTKLDE+ SYG +FN F +SY+ Sbjct: 130 RLHNILVLSATTKDSDTNEITRKFGSMPLDSIVFTKLDESSSYGSIFNHAIRFKKPVSYL 189 Query: 452 TNGQNVPDDLLAATKDQLSGMILGTGG 478 T GQNVP+D+ ATK++L ++L G Sbjct: 190 TTGQNVPEDIELATKERLVDLLLHISG 216 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.316 0.131 0.360 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 562,372,296 Number of extensions: 19933726 Number of successful extensions: 91783 Number of sequences better than 1.0e-05: 984 Number of HSP's gapped: 90456 Number of HSP's successfully gapped: 984 Length of query: 479 Length of database: 757,237,578 Length adjustment: 129 Effective length of query: 350 Effective length of database: 406,989,807 Effective search space: 142446432450 Effective search space used: 142446432450 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 128 (53.9 bits)