BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0586.0034.p (165 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_243299.1| methylation of methyl-accepting chemotaxis prot... 164 6e-40 ref|ZP_00059986.1| COG1871: Chemotaxis protein; stimulates methy... 148 4e-35 ref|NP_389528.1| alternate gene name: ylxK [Bacillus subtilis] >... 147 8e-35 ref|NP_692502.1| CheD [Oceanobacillus iheyensis HTE831] >gi|2277... 147 1e-34 ref|ZP_00183586.1| hypothetical protein Exigu101801 [Exiguobacte... 141 4e-33 >ref|NP_243299.1| methylation of methyl-accepting chemotaxis protein [Bacillus halodurans] pir||A83954 methylation of methyl-accepting chemotaxis protein cheD [imported] - Bacillus halodurans (strain C-125) dbj|BAB06152.1| methylation of methyl-accepting chemotaxis protein [Bacillus halodurans] Length = 162 Score = 164 bits (415), Expect = 6e-40, Method: Compositional matrix adjust. Identities = 84/161 (52%), Positives = 104/161 (64%) Query: 5 QSVIKVGMADLNVGSEESLVRTTGLGSCVGVTLFDPGKKLAGMAHVMLPSSEIAREGQMN 64 V+KVGMADLNV + +RT GLGSCVGV L+D KK+AGMAH+MLP S + ++ + N Sbjct: 2 NDVVKVGMADLNVVTPPHTIRTAGLGSCVGVVLYDDVKKVAGMAHIMLPDSALGKKQEFN 61 Query: 65 IAKFADTALPEXXXXXXXXXXXXXXXXXKMAGGSQMFAFAGGSDTMRIGPRNVESCKLAL 124 AK+ADTAL KMAGG+QMF+FA +D MRIG RNVE+ K L Sbjct: 62 RAKYADTALDLLLSKLEAIGAKRYSLKAKMAGGAQMFSFASNNDMMRIGQRNVEAVKKKL 121 Query: 125 EALKIPLIAEDTGGNFGRTIEIACNTGILYIRSVQKGTKEI 165 L IP++AED GG+ GRTIE T L IR+V +G KEI Sbjct: 122 RELSIPILAEDVGGSNGRTIEFNPETKRLSIRTVHQGEKEI 162 >ref|ZP_00059986.1| COG1871: Chemotaxis protein; stimulates methylation of MCP proteins [Clostridium thermocellum ATCC 27405] Length = 161 Score = 148 bits (374), Expect = 4e-35, Method: Compositional matrix adjust. Identities = 71/160 (44%), Positives = 102/160 (63%), Gaps = 1/160 (0%) Query: 6 SVIKVGMADLNVGSEESLVRTTGLGSCVGVTLFDPGKKLAGMAHVMLPSSEIAREGQMNI 65 ++I++GMADL +++ T GLGSCVG+ L+D K+ G+AH MLP S + + N Sbjct: 3 NIIRIGMADLKASHHPAILTTLGLGSCVGIALYDRTTKIVGLAHAMLPDSSQVKN-KTNA 61 Query: 66 AKFADTALPEXXXXXXXXXXXXXXXXXKMAGGSQMFAFAGGSDTMRIGPRNVESCKLALE 125 AKF DTA+ K+AGG+QMF F G++ MRIG RNV + K LE Sbjct: 62 AKFVDTAIELLVKEMLSLGASKKRIVAKLAGGAQMFTFTQGAELMRIGQRNVAAAKSKLE 121 Query: 126 ALKIPLIAEDTGGNFGRTIEIACNTGILYIRSVQKGTKEI 165 +L IP+++EDTGGN+GRT+E+ +G+L IR++ GTK+I Sbjct: 122 SLDIPVVSEDTGGNYGRTVELDSESGMLLIRTIGYGTKQI 161 >ref|NP_389528.1| alternate gene name: ylxK [Bacillus subtilis] sp|P40404|CHED_BACSU Chemotaxis protein cheD pir||B55216 chemotaxis methylation system protein cheD - Bacillus subtilis gb|AAA61469.1| ORFB emb|CAB13519.1| cheD [Bacillus subtilis subsp. subtilis str. 168] Length = 166 Score = 147 bits (371), Expect = 8e-35, Method: Compositional matrix adjust. Identities = 73/162 (45%), Positives = 103/162 (63%) Query: 4 EQSVIKVGMADLNVGSEESLVRTTGLGSCVGVTLFDPGKKLAGMAHVMLPSSEIAREGQM 63 E VIKVG+AD+ + +RT+GLGSCVG+ L+D K+ AG+ HVMLP S +++ ++ Sbjct: 5 EAVVIKVGIADVKIARFPDTIRTSGLGSCVGLVLYDKEKQTAGLVHVMLPDSTLSKTAEL 64 Query: 64 NIAKFADTALPEXXXXXXXXXXXXXXXXXKMAGGSQMFAFAGGSDTMRIGPRNVESCKLA 123 N AK+ADTA+ K+AGGS+MF F +D M+IGPRNV + K Sbjct: 65 NRAKYADTAVQTTIDMLIEAGCRKFALKAKLAGGSEMFKFKSTNDLMKIGPRNVLAIKEQ 124 Query: 124 LEALKIPLIAEDTGGNFGRTIEIACNTGILYIRSVQKGTKEI 165 L IP+I+EDTGG+ GRTIE + +L+IR+V++G K I Sbjct: 125 LSLFNIPIISEDTGGSSGRTIEFEPKSCMLHIRTVKQGEKTI 166 >ref|NP_692502.1| CheD [Oceanobacillus iheyensis HTE831] dbj|BAC13537.1| methylation of methyl-accepting chemotaxis protein [Oceanobacillus iheyensis HTE831] Length = 166 Score = 147 bits (370), Expect = 1e-34, Method: Compositional matrix adjust. Identities = 69/157 (43%), Positives = 99/157 (63%) Query: 4 EQSVIKVGMADLNVGSEESLVRTTGLGSCVGVTLFDPGKKLAGMAHVMLPSSEIAREGQM 63 E +V+KVG+ADL + L+RT+GLGSCVG ++D K++AG++HV+LP S+ ++ Sbjct: 5 ETTVVKVGIADLKIIQFPQLIRTSGLGSCVGTVIYDDKKEIAGLSHVLLPYSQQGKQANT 64 Query: 64 NIAKFADTALPEXXXXXXXXXXXXXXXXXKMAGGSQMFAFAGGSDTMRIGPRNVESCKLA 123 N K+ADTA+ K+AGG+QMF GSD MRIGPRNVES +LA Sbjct: 65 NPYKYADTAISYLYNELLKRGANQSSLKAKLAGGAQMFQLRTGSDLMRIGPRNVESVELA 124 Query: 124 LEALKIPLIAEDTGGNFGRTIEIACNTGILYIRSVQK 160 L IP++++D GGN GRTIE + +L +R + + Sbjct: 125 LAEFGIPIVSKDVGGNLGRTIEFDPRSKLLAVRKINQ 161 >ref|ZP_00183586.1| hypothetical protein Exigu101801 [Exiguobacterium sp. 255-15] Length = 164 Score = 141 bits (356), Expect = 4e-33, Method: Compositional matrix adjust. Identities = 74/159 (46%), Positives = 98/159 (61%), Gaps = 2/159 (1%) Query: 7 VIKVGMADLNVGSEESLVRTTGLGSCVGVTLFDPGKKLAGMAHVMLPSSEIAREGQMNIA 66 V+++G+A+ V + ++RT GLGSCVGV ++D + L+ MAHVMLP S I+R + I Sbjct: 8 VVRIGIAEYAVSQKPIILRTAGLGSCVGVIIYDQERGLSSMAHVMLPDSTISRNIALEIG 67 Query: 67 KFADTALPEXXXXXXXXXXXXXXXXXKMAGGSQMFAFAGGSDTMRIGPRNVESCKLALEA 126 KFADTA+ E K+AGG+QMF F ++MRIG RN+ + KLAL Sbjct: 68 KFADTAVVELARRLRQSGAIRLRA--KIAGGAQMFQFKYEHESMRIGERNIAAVKLALRK 125 Query: 127 LKIPLIAEDTGGNFGRTIEIACNTGILYIRSVQKGTKEI 165 +PL+AED GG GRTIE TG L IR+V GT EI Sbjct: 126 ANVPLVAEDVGGTNGRTIEFHSQTGRLVIRTVNVGTLEI 164 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.317 0.134 0.375 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 200,571,498 Number of extensions: 6792837 Number of successful extensions: 13469 Number of sequences better than 1.0e-05: 47 Number of HSP's gapped: 13443 Number of HSP's successfully gapped: 47 Length of query: 165 Length of database: 757,237,578 Length adjustment: 116 Effective length of query: 49 Effective length of database: 442,286,094 Effective search space: 21672018606 Effective search space used: 21672018606 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 121 (51.2 bits)