BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0584.0064.p (245 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gb|EAG41670.1| unknown [environmental sequence] 70 3e-11 ref|ZP_00092284.1| COG1011: Predicted hydrolase (HAD superfamily... 70 6e-11 ref|NP_390772.1| similar to hypothetical proteins [Bacillus subt... 68 1e-10 ref|ZP_00082995.1| COG1011: Predicted hydrolase (HAD superfamily... 68 2e-10 ref|NP_644286.1| hydrolase [Xanthomonas axonopodis pv. citri str... 67 4e-10 >gb|EAG41670.1| unknown [environmental sequence] Length = 182 Score = 70.5 bits (171), Expect = 3e-11, Method: Compositional matrix adjust. Identities = 42/143 (29%), Positives = 77/143 (53%), Gaps = 14/143 (9%) Query: 99 CHEAVDILFAEHRLSGLYADTVPFLQKISEAYQICIVS----DTDTLMLPDFYKEYPIKL 154 C EA + A H+L+ ++ + P L+ + + + + +++ D + L D++ + ++ Sbjct: 45 CFEA--FIHARHQLT-VFPEAEPMLKALRQHFLLGVITNGNADVQRVGLADYF-HFALR- 99 Query: 155 FTSEDYGSYKNDGRNRMFTEVIAYYGVQPEEILHIGD-SASDVLGAARAGIRSCWINRTG 213 +ED G K D R +F E + GV +H+GD D+ GA +AG+R+ W N TG Sbjct: 100 --AEDIGIAKPDAR--LFHEALQRGGVDASAAVHVGDHPGDDIAGAQQAGLRAVWFNPTG 155 Query: 214 SSWKHTVQPDYTVSNLEELYPIL 236 +W+ +PD + +L EL P+L Sbjct: 156 KAWEGDKRPDAQIRSLTELPPLL 178 >ref|ZP_00092284.1| COG1011: Predicted hydrolase (HAD superfamily) [Azotobacter vinelandii] Length = 233 Score = 69.7 bits (169), Expect = 6e-11, Method: Compositional matrix adjust. Identities = 44/136 (32%), Positives = 69/136 (50%), Gaps = 12/136 (8%) Query: 106 LFAEHRLSGLYADTVPFLQKISEAYQICIVS----DTDTLMLPDFYKEYPIKLFTSEDYG 161 L A HR+ + +T P L +++ Y++ +++ D L L D++ + +ED G Sbjct: 99 LAARHRIE-FFPETRPTLAILADRYRLGVLTNGNADVRRLGLADYFH----FILCAEDLG 153 Query: 162 SYKNDGRNRMFTEVIAYYGVQPEEILHIGDSASD-VLGAARAGIRSCWINRTGSSWKHTV 220 K D + F E + G EE +HIGD D + GA RAG+R+ W N G W H Sbjct: 154 IGKPD--PQPFHEALRRGGASAEETVHIGDHPDDDIQGAQRAGLRAIWYNPGGKPWSHAG 211 Query: 221 QPDYTVSNLEELYPIL 236 PD +++L EL +L Sbjct: 212 TPDAQIASLAELPALL 227 >ref|NP_390772.1| similar to hypothetical proteins [Bacillus subtilis] pir||B69983 conserved hypothetical protein ysaA - Bacillus subtilis emb|CAA99609.1| hypothetical protein [Bacillus subtilis] emb|CAB14854.1| ysaA [Bacillus subtilis subsp. subtilis str. 168] Length = 260 Score = 68.2 bits (165), Expect = 1e-10, Method: Compositional matrix adjust. Identities = 40/134 (29%), Positives = 73/134 (54%), Gaps = 17/134 (12%) Query: 115 LYADTVPFLQKISEAYQICIVSDTDTLMLPDFYKE----------YPIKLFTSEDYGSYK 164 +Y +T L ++ Y++ ++++ D P KE Y ++ S +G K Sbjct: 132 VYDETFAVLDQLKGKYELLLLTNGD----PSLQKEKLAGVPELAPYFNEIVISGAFGKGK 187 Query: 165 NDGRNRMFTEVIAYYGVQPEEILHIGDS-ASDVLGAARAGIRSCWINRTGSSWKHTVQPD 223 D +F + ++ ++ + +GD+ +D+LGA+RAGI++ WINRT + V+PD Sbjct: 188 PD--VSIFEHCLKLMNIEKDDAIMVGDNLNTDILGASRAGIKTVWINRTDKKNETDVKPD 245 Query: 224 YTVSNLEELYPILE 237 Y +S+L +L+PILE Sbjct: 246 YIISSLHDLFPILE 259 >ref|ZP_00082995.1| COG1011: Predicted hydrolase (HAD superfamily) [Pseudomonas fluorescens PfO-1] Length = 236 Score = 68.2 bits (165), Expect = 2e-10, Method: Compositional matrix adjust. Identities = 42/136 (30%), Positives = 69/136 (50%), Gaps = 12/136 (8%) Query: 106 LFAEHRLSGLYADTVPFLQKISEAYQICIVS----DTDTLMLPDFYKEYPIKLFTSEDYG 161 L A H++ ++ + P L+ +++ Y + +V+ D L L D++K +ED G Sbjct: 101 LHARHQIE-VFPEVEPILETLAKHYALGVVTNGNADVRRLGLADYFK----FALCAEDIG 155 Query: 162 SYKNDGRNRMFTEVIAYYGVQPEEILHIGD-SASDVLGAARAGIRSCWINRTGSSWKHTV 220 K D R +F E + GV E +HIGD D+ GA +AG+R+ W N G W+ Sbjct: 156 IAKPDAR--LFHEALQRGGVSAEAAVHIGDHPGDDIAGAQQAGLRAIWFNPAGKVWEAER 213 Query: 221 QPDYTVSNLEELYPIL 236 PD + +L +L +L Sbjct: 214 LPDAEIRSLNDLPAVL 229 >ref|NP_644286.1| hydrolase [Xanthomonas axonopodis pv. citri str. 306] gb|AAM38822.1| hydrolase [Xanthomonas axonopodis pv. citri str. 306] Length = 245 Score = 66.6 bits (161), Expect = 4e-10, Method: Compositional matrix adjust. Identities = 45/143 (31%), Positives = 69/143 (48%), Gaps = 6/143 (4%) Query: 102 AVDILFAEHRLSGLYADTVPFLQKISEAYQICIVSDTDT-LMLPDFYKEYPIKLFTSEDY 160 A ++ +A Y D + L +I+ + +S+ + L + +L S ++ Sbjct: 93 AYEVFYAARNQVECYPDALDALARIAAHVPVAALSNGNADLQRIGLMHHFAFQL-GSREH 151 Query: 161 GSYKNDGRNRMFTEVIAYYGVQPEEILHIGDSAS-DVLGAARAGIRSCWINRTGSSWKH- 218 GS K D +F A V P ++LH+GD DVLGA AG+R+CWINR G+ W H Sbjct: 152 GSAKPD--PSIFLAACARLEVPPAQVLHVGDHVRMDVLGALDAGLRACWINREGAVWSHP 209 Query: 219 TVQPDYTVSNLEELYPILEPGAP 241 T QPD ++ L L+ P Sbjct: 210 TQQPDLQFDSMTGLADWLDAQQP 232 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.320 0.136 0.406 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 365,379,756 Number of extensions: 14571536 Number of successful extensions: 34585 Number of sequences better than 1.0e-05: 24 Number of HSP's gapped: 34751 Number of HSP's successfully gapped: 24 Length of query: 245 Length of database: 757,237,578 Length adjustment: 122 Effective length of query: 123 Effective length of database: 425,995,500 Effective search space: 52397446500 Effective search space used: 52397446500 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 124 (52.4 bits)