BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0584.0063.p (187 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value emb|CAC34051.1| cellulase [Alicyclobacillus acidocaldarius] 146 2e-34 sp|P23658|GUNC_BUTFI Cellodextrinase >gi|97314|pir||S12025 cello... 136 2e-31 dbj|BAC65236.1| cellulase [Clostridium sp. JC3] 115 7e-25 gb|AAG49558.1| endo-1,4-beta-D-glucanase precursor [Pseudomonas ... 114 1e-24 gb|AAC06139.1| cellulose 1,4-beta-cellobiosidase [Clostridium th... 112 4e-24 >emb|CAC34051.1| cellulase [Alicyclobacillus acidocaldarius] Length = 537 Score = 146 bits (369), Expect = 2e-34, Method: Compositional matrix adjust. Identities = 81/181 (44%), Positives = 99/181 (54%), Gaps = 1/181 (0%) Query: 4 YGTLAYLLSGESAAQTALYASLKQGLLAEADRLVRLSRDDGYRISLREEDYIWGSXXXXX 63 YG + YL + +A + +K LL E D L ++ + I +R++D+IWGS Sbjct: 349 YGVMDYLRTPRAAVSDDVRNKVKSRLLRELDALAAMAESHPFGIPMRDDDFIWGSNMVLL 408 Query: 64 XXXXXXXXXEHFSG-NIAYADCALDHLHYLMGRNVLDISYVTGFGDRPVMHPHHRPSVGD 122 E + A A YL G N L YVTGFG RPV HPHHRPSV D Sbjct: 409 NRAMAFLLAEGVGVLHPAAHTVAQRAADYLFGANPLGQCYVTGFGQRPVRHPHHRPSVAD 468 Query: 123 GVADPVPGMVSGGPDRGLHDEYVALHLQGKPAAQCFADHDLSYSTNEVTIYWNSPAVFVT 182 V PVPGMV GGP+R L DE L G+PA + + DH SYSTNEV +YWNSPAVFV Sbjct: 469 DVDHPVPGMVVGGPNRHLQDEIARAQLAGRPAMEAYIDHQDSYSTNEVAVYWNSPAVFVI 528 Query: 183 A 183 A Sbjct: 529 A 529 >sp|P23658|GUNC_BUTFI Cellodextrinase pir||S12025 cellodextrinase - Butyrivibrio fibrisolvens emb|CAA39264.1| cellodextrinase [Butyrivibrio fibrisolvens] Length = 547 Score = 136 bits (343), Expect = 2e-31, Method: Compositional matrix adjust. Identities = 67/164 (40%), Positives = 95/164 (57%), Gaps = 1/164 (0%) Query: 18 QTALYASLKQGLLAEADRLVRLSRDDGYRISLREEDYIWGSXXXXXXXXXXXXXXEHFSG 77 + AL + + +AEADRLV++S+++G+ + + E D+IWGS Sbjct: 377 ENALCSLARNSFVAEADRLVKVSKENGFGLCMGENDFIWGSNMELLKYMMVLSTAIRIDN 436 Query: 78 NIAYADCALDHLHYLMGRNVLDISYVTGFGDRPVMHPHHRPSVGDGVADPVPGMVSGGPD 137 Y L Y++G N +DISYVTG G++ +PH RP+ D + +P PG+VSGGP+ Sbjct: 437 KPEYKLALEAGLDYILGCNSMDISYVTGNGEKAFKNPHLRPTAVDDIEEPWPGLVSGGPN 496 Query: 138 RGLHDEYV-ALHLQGKPAAQCFADHDLSYSTNEVTIYWNSPAVF 180 GLHDE L +G P +C+ DH YS NE+TIYWNSP VF Sbjct: 497 SGLHDERAQTLRGKGLPPMKCYIDHIDCYSLNEITIYWNSPLVF 540 >dbj|BAC65236.1| cellulase [Clostridium sp. JC3] Length = 366 Score = 115 bits (287), Expect = 7e-25, Method: Compositional matrix adjust. Identities = 64/191 (33%), Positives = 96/191 (50%), Gaps = 10/191 (5%) Query: 2 GGYGTLAYLLSGESAAQTALYASLKQGLLAEADRLVRLSRDDGYRISLREED-----YIW 56 G GT++ L + + + + + AD + D GYR+ +++ + Y W Sbjct: 54 AGLGTVSLALVPNGLPSSEIQKA-RNNIAKAADAWLDNIEDQGYRLPIKQAEDERGGYPW 112 Query: 57 GSXXXXXXXXXXXXXXEHFSGNIAYADCALDHLHYLMGRNVLDISYVTGFGDRPVMHPHH 116 GS F+GN Y D D + YL+GRN +D SYVTG+G+RP+ +PH Sbjct: 113 GSNSFILNMMIVMAYARDFTGNDKYFDGIQDSISYLLGRNAMDQSYVTGYGERPLTNPHD 172 Query: 117 R---PSVGDGVADPVPGMVSGGPDRGLHDEYV-ALHLQGKPAAQCFADHDLSYSTNEVTI 172 R P P G++SGGP+ D + A L+ P +CF DH S+STNE+T+ Sbjct: 173 RFWTPQTSAKWPAPPAGIISGGPNSRFEDPTINAAVLKDTPPQKCFIDHTDSWSTNEITV 232 Query: 173 YWNSPAVFVTA 183 WN+P +VTA Sbjct: 233 NWNAPFAWVTA 243 >gb|AAG49558.1| endo-1,4-beta-D-glucanase precursor [Pseudomonas sp. SK38] Length = 506 Score = 114 bits (286), Expect = 1e-24, Method: Compositional matrix adjust. Identities = 64/168 (38%), Positives = 89/168 (52%), Gaps = 6/168 (3%) Query: 15 SAAQTALYASLKQGLLAEADRLVRLSRDDGYRISLREEDYIWGSXXXXXXXXXXXXXXEH 74 +AA TAL +++ + AD +V + GY +S+ D++WGS Sbjct: 332 AAADTAL---IQKRVTDLADTIVAAGNEVGYGVSMTRPDFVWGSNGVAMNRAMMLVQAYR 388 Query: 75 FSGNIAYADCALDHLHYLMGRNVLDISYVTGFGDRPVMHPHHRPSVGDGVADPVPGMVSG 134 +G Y A L Y++GRN + S+VTGFG RP MH HHRPSV D V PVPG ++G Sbjct: 389 ITGEQNYMAAAQALLDYVLGRNPTEYSFVTGFGARPPMHIHHRPSVADDVVAPVPGFLAG 448 Query: 135 GPDRGLHDEYVALHLQGKPAAQCFADHDLSYSTNEVTIYWNSPAVFVT 182 GP G D A+ + D SY++NE+TI WN+P V+VT Sbjct: 449 GPHSGQQD---GCEYPSDLPAESYLDDWCSYASNEITINWNAPLVYVT 493 >gb|AAC06139.1| cellulose 1,4-beta-cellobiosidase [Clostridium thermocellum] Length = 895 Score = 112 bits (280), Expect = 4e-24, Method: Composition-based stats. Identities = 63/190 (33%), Positives = 96/190 (50%), Gaps = 10/190 (5%) Query: 3 GYGTLAYLLSGESAAQTALYASLKQGLLAEADRLVRLSRDDGYRISLREED-----YIWG 57 G GT+ L T + + + + ADR + + GYR+ +++ + Y WG Sbjct: 621 GLGTITLALVENGLPATDIQKA-RNNIAKAADRWLENIEEQGYRLPIKQAEDERGGYPWG 679 Query: 58 SXXXXXXXXXXXXXXEHFSGNIAYADCALDHLHYLMGRNVLDISYVTGFGDRPVMHPHHR 117 S F+GN Y D D + YL+GRN LD SYVTG+G+RP+ +PH R Sbjct: 680 SNSFILNQMIVMGYAYDFTGNSKYLDGMQDGMSYLLGRNGLDQSYVTGYGERPLQNPHDR 739 Query: 118 ---PSVGDGVADPVPGMVSGGPDRGLHDEYVALHL-QGKPAAQCFADHDLSYSTNEVTIY 173 P P PG+++GGP+ D + + + P +C+ DH S+STNE+T+ Sbjct: 740 FWTPQTSKKFPAPPPGIIAGGPNSRFEDPTITAAVKKDTPPQKCYIDHTDSWSTNEITVN 799 Query: 174 WNSPAVFVTA 183 WN+P +VTA Sbjct: 800 WNAPFAWVTA 809 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.320 0.138 0.429 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 278,648,452 Number of extensions: 11347229 Number of successful extensions: 21307 Number of sequences better than 1.0e-05: 36 Number of HSP's gapped: 21262 Number of HSP's successfully gapped: 36 Length of query: 187 Length of database: 757,237,578 Length adjustment: 118 Effective length of query: 69 Effective length of database: 436,855,896 Effective search space: 30143056824 Effective search space used: 30143056824 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 122 (51.6 bits)