BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0584.0059.p (322 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_00061851.1| COG2206: HD-GYP domain [Clostridium thermocel... 186 6e-46 ref|NP_240874.1| BH0008~unknown conserved protein in others [Bac... 156 8e-37 ref|NP_350232.1| HD-GYP domain containing protein [Clostridium a... 139 1e-31 ref|NP_244661.1| BH3794~unknown conserved protein in others [Bac... 124 2e-27 ref|NP_349227.1| HD-GYP hydrolase domain containing protein [Clo... 121 2e-26 >ref|ZP_00061851.1| COG2206: HD-GYP domain [Clostridium thermocellum ATCC 27405] Length = 345 Score = 186 bits (472), Expect = 6e-46, Method: Compositional matrix adjust. Identities = 95/282 (33%), Positives = 160/282 (56%), Gaps = 1/282 (0%) Query: 41 SGLGHSLNDNFDHAIQNYESIFLEALAQGTFTQSMVDDTLQPLLETLDTQKDVVSLLLLL 100 S + S N +++ ++ ++SI +A VD + ++ LD ++D+V + + Sbjct: 64 SEISESFNVSYNASVNCFKSILYDAAVGKNINMEKVDKVVMSVVARLDEKRDIVRCINQI 123 Query: 101 ERDDIDTYHHSLQVGLLSYYIAAWMGHSKEERYEISRAGYLHDIGKSQVPLSILNKAGIL 160 + D TY H + V LL + W+G+SK+ ++ +AG LHDIGK++VP ILNK L Sbjct: 124 KSADQYTYTHCVNVSLLCMLLGKWLGYSKDSLIKLVQAGLLHDIGKAKVPKEILNKPSKL 183 Query: 161 TPAEEEELKRHTTFGYEII-RGSKMDEKTALVALQHHEFEDGTGYPNQILKSGIHPYTEI 219 TP E EE+K+HT +GY I+ + ++D A+ L HHE DG+GYP I+ + Sbjct: 184 TPEEYEEIKKHTVYGYRILEKEHEIDRDVAIAVLMHHERMDGSGYPTGAKSDQINMMARV 243 Query: 220 VTVANIYMGLTTSRLNQPKQGLVAVLRKVHEMGFGKLNGTVVQALTGHLLPGFVGKNVQL 279 + VA+IY +T++R+ + K+ V + E GKL+ VV A ++ ++G +L Sbjct: 244 IAVADIYDAMTSNRVYREKESPFEVFHIIEEEAVGKLDVRVVNAFLSNIAAYYIGDFAKL 303 Query: 280 SNGEIGTIVMNNPLDMFRPLVKVDNVFKDLSRERSLSVEEII 321 + GEI IV NP + RP+++VD+ + DL+ E+ L + EI+ Sbjct: 304 NTGEICEIVYINPRYVSRPIIRVDDQYIDLTVEKDLKILEIL 345 >ref|NP_240874.1| BH0008~unknown conserved protein in others [Bacillus halodurans] pir||H83650 hypothetical protein BH0008 [imported] - Bacillus halodurans (strain C-125) dbj|BAA82692.1| ORF14 [Bacillus halodurans] dbj|BAB03727.1| BH0008 [Bacillus halodurans] Length = 360 Score = 156 bits (394), Expect = 8e-37, Method: Compositional matrix adjust. Identities = 99/310 (31%), Positives = 163/310 (52%), Gaps = 15/310 (4%) Query: 8 KRTVVQREEITILLRHKIEYVDIEPRTASHAE----DSGLGHS----------LNDNFDH 53 ++TV+ E+I L + I+ +++E A+ + D+GLG D + Sbjct: 29 EKTVLTAEDIQTLSKFLIDEIEVENVLANGEKFIPSDNGLGAEAAALMETGDRFKDRYMQ 88 Query: 54 AIQNYESIFLEALAQGTFTQSMVDDTLQPLLETLDTQKDVVSLLLLLERDDIDTYHHSLQ 113 A+ NY+ +F E A V L PL E + +KDV+ L + YHH++ Sbjct: 89 AVANYKKVFQEWAAGTPVEMECVRHILLPLFEEIVEKKDVLLQLHHYSTKEDYLYHHAVS 148 Query: 114 VGLLSYYIAAWMGHSKEERYEISRAGYLHDIGKSQVPLSILNKAGILTPAEEEELKRHTT 173 VGLLS ++ A +G+ K E +I+ AG L D G +++P S+L K G+ T E+ E++ H Sbjct: 149 VGLLSAFLGAKLGYEKGEWLKIALAGALADCGMAKLPTSLLTKNGLYTLKEKHEMRDHPK 208 Query: 174 FGYEIIRGSKMDEKTALVA-LQHHEFEDGTGYPNQILKSGIHPYTEIVTVANIYMGLTTS 232 + Y++++ + + L A LQHHE EDGTGYP + IH Y +I+ VA++Y + + Sbjct: 209 YSYQMLKKRPEIDGSLLSAVLQHHEREDGTGYPLGVRGQKIHFYAQILAVADVYHAMISE 268 Query: 233 RLNQPKQGLVAVLRKVHEMGFGKLNGTVVQALTGHLLPGFVGKNVQLSNGEIGTIVMNNP 292 R + K+ VL + + FGK N +V L L V +V+L+N +G IV +P Sbjct: 269 RKYRKKKSPYMVLEDLAKERFGKFNPRIVHVLVSQLTQLSVRTSVRLTNNRLGEIVFIDP 328 Query: 293 LDMFRPLVKV 302 + RP+V++ Sbjct: 329 MAPTRPMVQL 338 >ref|NP_350232.1| HD-GYP domain containing protein [Clostridium acetobutylicum] pir||A97348 HD-GYP domain containing protein [imported] - Clostridium acetobutylicum gb|AAK81572.1| HD-GYP domain containing protein [Clostridium acetobutylicum] Length = 355 Score = 139 bits (349), Expect = 1e-31, Method: Compositional matrix adjust. Identities = 71/216 (32%), Positives = 123/216 (56%), Gaps = 1/216 (0%) Query: 107 TYHHSLQVGLLSYYIAAWMGHSKEERYEISRAGYLHDIGKSQVPLSILNKAGILTPAEEE 166 T++HS+ V IA WM S+++ E+ AG LHD+GK+++ SILNK G LT E E Sbjct: 138 TFYHSVNVSFYGMLIAKWMNLSEKQIREVISAGLLHDLGKTKIKNSILNKPGRLTDEEFE 197 Query: 167 ELKRHTTFGYEIIRGS-KMDEKTALVALQHHEFEDGTGYPNQILKSGIHPYTEIVTVANI 225 E+K+H +GYEII+ +++ V LQHHE +GTGYP I + Y+ I+ +A++ Sbjct: 198 EMKKHPLYGYEIIKDDPTINDDIKNVILQHHERINGTGYPYGIKADEMGLYSRIIAIADV 257 Query: 226 YMGLTTSRLNQPKQGLVAVLRKVHEMGFGKLNGTVVQALTGHLLPGFVGKNVQLSNGEIG 285 Y +T+ R+ + K+ G + +++ ++ F+G V L++G G Sbjct: 258 YDAMTSDRVYKKKKSPFRAFEMFKTEGISIFDTDILRVFLNNINVYFIGAEVLLNDGRKG 317 Query: 286 TIVMNNPLDMFRPLVKVDNVFKDLSRERSLSVEEII 321 IV P ++ +P++K+++ + D S++ S+ E+I Sbjct: 318 KIVYIPPNNLLKPILKMNSTYIDFSKDNGYSISEVI 353 >ref|NP_244661.1| BH3794~unknown conserved protein in others [Bacillus halodurans] pir||B84124 hypothetical protein BH3794 [imported] - Bacillus halodurans (strain C-125) dbj|BAB07513.1| BH3794~unknown conserved protein in others [Bacillus halodurans] Length = 231 Score = 124 bits (312), Expect = 2e-27, Method: Compositional matrix adjust. Identities = 80/224 (35%), Positives = 124/224 (55%), Gaps = 9/224 (4%) Query: 102 RDDIDTYHHSLQVGLLSYYIAAWMGHSKEERYEISRAGYLHDIGKSQVPLSILNKAGILT 161 +DD TY H++ V +LS I W+ + E++ LHDIGK ++P +ILNK G LT Sbjct: 7 KDDY-TYRHTVGVSILSRLIGTWLKLDEVILKELTLGAVLHDIGKVEIPDAILNKPGALT 65 Query: 162 PAEEEELKRHTTFGYEIIRGSKMDEKT-ALVALQHHEFEDGTGYPNQILKSGIHPYTEIV 220 E ++ HT GY II+ S + T AL+AL+HHE EDGTGYP Q ++ IH ++I+ Sbjct: 66 KDEYRIIQDHTVKGYRIIQESGIYSDTVALMALEHHEREDGTGYPYQKTRNEIHLLSKIL 125 Query: 221 TVANIYMGLTTSRLNQPKQGLVAVLRKVHEMGFGKLNGTVVQALTGHLLPGFVGKNVQLS 280 VA+++ +T+ R+ + L VL ++ + FGKL + + +G +L+ Sbjct: 126 AVADVFHAMTSDRIYRKGMALYDVLLQMRQDRFGKLEPRITDFFVRRFMEQCIGSKARLN 185 Query: 281 NG---EIGTIVMNNPLDMFRPLVKVDNVFKDLSRERSLSVEEII 321 +G EI I NP+ P+VK + DL R L ++E+I Sbjct: 186 DGSRVEIVFIPYENPIC---PIVKQGTNYIDL-RMSPLYIQELI 225 >ref|NP_349227.1| HD-GYP hydrolase domain containing protein [Clostridium acetobutylicum] pir||D97222 HD-GYP hydrolase domain containing protein [imported] - Clostridium acetobutylicum gb|AAK80567.1| HD-GYP hydrolase domain containing protein [Clostridium acetobutylicum] Length = 345 Score = 121 bits (304), Expect = 2e-26, Method: Compositional matrix adjust. Identities = 68/218 (31%), Positives = 117/218 (53%), Gaps = 5/218 (2%) Query: 106 DTYHHSLQVGLLSYYIAAWMGHSKEERYEISRAGYLHDIGKSQVPLSILNKAGILTPAEE 165 + Y HS+ V +S+ + W+G ++EE ++ A LHD GK+Q+ SIL K LTP E Sbjct: 132 NIYRHSVNVAAISFILGKWLGMNEEEINLLTYASLLHDFGKTQLDNSILKKESSLTPEEY 191 Query: 166 EELKRHTTFGYEIIRG-SKMDEKTALVALQHHEFEDGTGYPNQILKSGIHPYTEIVTVAN 224 K H Y +I+ ++ L L HHE DG+GYP I + IH + +I+ +A+ Sbjct: 192 AVYKTHPVIAYHLIKEIPDINASVGLGVLMHHEQIDGSGYPLGIKDNKIHKFAKIIAIAD 251 Query: 225 IYMGLTTSRLNQPKQGLVAVLRKVHEMGFGKLNGTVVQALTGHLLPGFVGKNVQLSNGEI 284 I+ + + N P +GL A + ++ GKL+ + H + ++G++V LS+ Sbjct: 252 IFDKVNSDNTNGPFEGLKA----IKDLSLGKLDCSYSNMFINHAVNYYMGESVTLSDNRS 307 Query: 285 GTIVMNNPLDMFRPLVKVDNVFKDLSRERSLSVEEIIM 322 I+ + ++ +PL+ D+ F DL E+ L V+ +I+ Sbjct: 308 CKIIKLDTENLTKPLLLADDGFLDLKSEKDLYVKSLIV 345 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.318 0.136 0.385 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 474,968,829 Number of extensions: 19943627 Number of successful extensions: 47158 Number of sequences better than 1.0e-05: 330 Number of HSP's gapped: 46946 Number of HSP's successfully gapped: 330 Length of query: 322 Length of database: 757,237,578 Length adjustment: 126 Effective length of query: 196 Effective length of database: 415,135,104 Effective search space: 81366480384 Effective search space used: 81366480384 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 126 (53.1 bits)