BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0584.0040.p (188 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_348292.1| Predicted membrane protein [Clostridium acetobu... 87 2e-16 ref|ZP_00097398.1| COG4905: Predicted membrane protein [Desulfit... 78 1e-13 ref|NP_472147.1| lin2818 [Listeria innocua] >gi|25373788|pir||AD... 74 2e-12 ref|NP_466191.1| lmo2669 [Listeria monocytogenes EGD-e] >gi|2537... 74 2e-12 ref|ZP_00060150.1| COG4905: Predicted membrane protein [Clostrid... 72 9e-12 >ref|NP_348292.1| Predicted membrane protein [Clostridium acetobutylicum] pir||E97105 probable membrane protein [imported] - Clostridium acetobutylicum gb|AAK79632.1| Predicted membrane protein [Clostridium acetobutylicum] Length = 238 Score = 87.0 bits (214), Expect = 2e-16, Method: Compositional matrix adjust. Identities = 49/142 (34%), Positives = 74/142 (52%), Gaps = 5/142 (3%) Query: 15 EYFFYFMLYSFLGWVLEGSYNLYSLGTFRKEGFLKGPFKPMYGFXXXXXXXXXXXXXXXX 74 EY +F +YSF+GWVLE + + GFL G F P+YGF Sbjct: 6 EYIIFFGIYSFIGWVLETIFASVNDKKIVNRGFLTGFFCPIYGFGAILTVQCSRMVGLSF 65 Query: 75 XXXXMILLALIIPSA-----VEYTSGLLLKSAFHKQWWDYSGMSYQLQGHICLKFSLYWW 129 + L++ +I S +EY +G +L++ F+ +WWDYS + + G+ICLK+SL W Sbjct: 66 KNPIISLISSVIFSIFLVTFLEYVTGFILEAIFNCKWWDYSNDAANIHGYICLKYSLLWG 125 Query: 130 GLATVCVYGLQPLIERLYLRIS 151 LA + V + PLI ++ IS Sbjct: 126 MLAFLLVQTIHPLISKMVFNIS 147 >ref|ZP_00097398.1| COG4905: Predicted membrane protein [Desulfitobacterium hafniense] Length = 239 Score = 77.8 bits (190), Expect = 1e-13, Method: Compositional matrix adjust. Identities = 49/141 (34%), Positives = 68/141 (48%), Gaps = 5/141 (3%) Query: 15 EYFFYFMLYSFLGWVLEGSYNLYSLGTFRKEGFLKGPFKPMYGFXXXXXXXX-----XXX 69 + F F YS LGW++E Y + F GFL G F P+YG Sbjct: 5 DIFLLFAWYSCLGWIMETIYASITHKKFVNRGFLNGFFCPIYGLGAVLIIVSSTWVGTIF 64 Query: 70 XXXXXXXXXMILLALIIPSAVEYTSGLLLKSAFHKQWWDYSGMSYQLQGHICLKFSLYWW 129 ++ A+I+ +A+EY +G +L+ FH +WWDYS L G+ICLK+SL W Sbjct: 65 SGALAAGTFSVVAAVILVTALEYITGFMLEKVFHCKWWDYSEDFANLHGYICLKYSLLWG 124 Query: 130 GLATVCVYGLQPLIERLYLRI 150 LA V + + P I L L I Sbjct: 125 VLALVLLEIVHPGIAGLLLFI 145 >ref|NP_472147.1| lin2818 [Listeria innocua] pir||AD1784 hypothetical protein lin2818 [imported] - Listeria innocua (strain Clip11262) emb|CAC98044.1| lin2818 [Listeria innocua] Length = 270 Score = 73.6 bits (179), Expect = 2e-12, Method: Compositional matrix adjust. Identities = 39/128 (30%), Positives = 60/128 (46%), Gaps = 2/128 (1%) Query: 16 YFFYFMLYSFLGWVLEGSYNLYSLGTFRKEGFLKGPFKPMYGFXXXXXXXXXXXXXXXXX 75 + YF +YS LGW E + S GFL GP+ P+YGF Sbjct: 6 FILYFFIYSVLGWAWEEVFCSISEKKLVYRGFLYGPYCPIYGFGVTTVLMMILPFQNNLW 65 Query: 76 XXXMILLALIIPSAVEYTSGLLLKSAFHKQWWDYSGMSYQLQGHICLKFSLYWWGLATVC 135 + + ++II + +EY + +L++ FH WWDY ++G ICL S++W + Sbjct: 66 A--LFIFSMIICTVIEYVTATILEALFHTTWWDYHNWPLNVKGRICLPISIFWGFACIIV 123 Query: 136 VYGLQPLI 143 V L PL+ Sbjct: 124 VRFLHPLV 131 >ref|NP_466191.1| lmo2669 [Listeria monocytogenes EGD-e] pir||AD1408 hypothetical protein lmo2669 [imported] - Listeria monocytogenes (strain EGD-e) emb|CAD00882.1| lmo2669 [Listeria monocytogenes] Length = 270 Score = 73.6 bits (179), Expect = 2e-12, Method: Compositional matrix adjust. Identities = 39/128 (30%), Positives = 60/128 (46%), Gaps = 2/128 (1%) Query: 16 YFFYFMLYSFLGWVLEGSYNLYSLGTFRKEGFLKGPFKPMYGFXXXXXXXXXXXXXXXXX 75 + YF +YS LGW E + S GFL GP+ P+YGF Sbjct: 6 FILYFFIYSVLGWAWEEVFCSISEKKLVYRGFLYGPYCPIYGFGVTAVLMMILPFQNNLW 65 Query: 76 XXXMILLALIIPSAVEYTSGLLLKSAFHKQWWDYSGMSYQLQGHICLKFSLYWWGLATVC 135 + + ++II + +EY + +L++ FH WWDY ++G ICL S++W + Sbjct: 66 A--LFIFSMIICTVIEYVTATILEALFHTTWWDYHNWPLNVKGRICLPISIFWGFACIIV 123 Query: 136 VYGLQPLI 143 V L PL+ Sbjct: 124 VRFLHPLV 131 >ref|ZP_00060150.1| COG4905: Predicted membrane protein [Clostridium thermocellum ATCC 27405] Length = 213 Score = 71.6 bits (174), Expect = 9e-12, Method: Compositional matrix adjust. Identities = 46/145 (31%), Positives = 63/145 (43%), Gaps = 31/145 (21%) Query: 15 EYFFYFMLYSFLGWVLEGSYNLYSLGTFRKEGFLKGPFKPMYGFXXXXXXXXXXXXXXXX 74 + YF+LY+F+GW+ E Y Y F G L GP P+YGF Sbjct: 23 DTVIYFVLYAFIGWISEVIYAAYEEKRFVNRGVLAGPICPIYGFGAVLILG--------- 73 Query: 75 XXXXMILLALIIPSAV----------------EYTSGLLLKSAFHKQWWDYSGMSYQLQG 118 + ALI P V EY +G +L+ F+ + WDYS + L G Sbjct: 74 ------IDALIKPGTVPVYVSVALAVVVTTVLEYITGYILEKNFNIKAWDYSNEEFNLHG 127 Query: 119 HICLKFSLYWWGLATVCVYGLQPLI 143 ICLKFSL W A + + + P+I Sbjct: 128 RICLKFSLLWGIFAYMLLTFIHPVI 152 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.329 0.146 0.496 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 203,426,778 Number of extensions: 6247486 Number of successful extensions: 14719 Number of sequences better than 1.0e-05: 13 Number of HSP's gapped: 14704 Number of HSP's successfully gapped: 13 Length of query: 188 Length of database: 757,237,578 Length adjustment: 118 Effective length of query: 70 Effective length of database: 436,855,896 Effective search space: 30579912720 Effective search space used: 30579912720 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 122 (51.6 bits)