BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0584.0028.p (363 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_832921.1| Low temperature requirement protein A [Bacillus... 397 e-109 ref|NP_469752.1| low temperature requirement protein A [Listeria... 252 8e-66 ref|NP_463919.1| low temperature requirement protein A [Listeria... 243 8e-63 gb|AAC98899.1| low temperature requirement A protein [Listeria m... 219 5e-56 ref|ZP_00111390.1| COG4292: Predicted membrane protein [Nostoc p... 143 7e-33 >ref|NP_832921.1| Low temperature requirement protein A [Bacillus cereus ATCC 14579] gb|AAP10122.1| Low temperature requirement protein A [Bacillus cereus ATCC 14579] Length = 363 Score = 397 bits (1021), Expect = e-109, Method: Compositional matrix adjust. Identities = 202/363 (55%), Positives = 263/363 (72%), Gaps = 1/363 (0%) Query: 1 MTVKKVTWLELFYDLLYVAAVSKASHVLLHVEDETLSLETLGKFVLIFIPIWWAWVGQTL 60 M KKVTWLELFYDLL+VAAV+ A+HVLLHVED + E L KFVLIFIPIWWAWVGQT+ Sbjct: 1 MEEKKVTWLELFYDLLFVAAVTAATHVLLHVEDGQIHAEYLVKFVLIFIPIWWAWVGQTI 60 Query: 61 FVNRYGQDIVSHRXXXXXXXXXXXXMTASLSVDFDQTYYSFLTGYLGLRAMTAVQYHYVR 120 FVNR+G+D+ R MT+SLS DFD Y SFL GY+GLR +TA+QY V+ Sbjct: 61 FVNRFGKDLFHQRIFLILQMFFVLIMTSSLSADFDPYYLSFLIGYIGLRVVTAIQYLVVQ 120 Query: 121 SKENGHLRATAHYLGSRFWIGLVISACSLFFDSWVRYAVLYAGIAVDIILPLAGRKILVR 180 E G + A +LG FWIG+VIS S+FFDSW+RYAVLY GI +DII PL GRK L + Sbjct: 121 RIEEGARKKAALFLGRYFWIGIVISLLSVFFDSWIRYAVLYFGILIDIIAPLLGRKCLEK 180 Query: 181 TPANTHHLLERFSLFALILLGESVVSILAVLQSSTWTLQSVGFAGLTFVLIIAMWWQYYD 240 P NT HLLERF LF++IL GE++VS L V+Q S+ FA ++F+LII+MWWQY+D Sbjct: 181 VPTNTAHLLERFGLFSIILFGEALVSTLVVIQPKQGNWDSISFAIISFILIISMWWQYFD 240 Query: 241 NLEKKVDKNVHTAGQTIIYGHLFIFLSLCMLAASIQLLFLNKLHYELMVNLLFGSVLLYF 300 N+EKK+DK+ T+GQ IIYGHLFI +SL M+AASI+LLFL++++Y ++ +FGSVLLYF Sbjct: 241 NMEKKLDKSRQTSGQIIIYGHLFILMSLSMVAASIRLLFLHEVYYSFILYFVFGSVLLYF 300 Query: 301 ASTTLVFHVYRHKQQXXXXXXXXXXXXXXXXCFYILDMAVHVPNVLIMGEMALFFAVYAR 360 STT+VFH YR +Q F+++++ + VPN++I+G++ LFF +YA+ Sbjct: 301 LSTTIVFHQYRFEQH-RLNIYHLCLFLGILAVFFVINLIIVVPNIVIIGQLTLFFIIYAK 359 Query: 361 ITS 363 +TS Sbjct: 360 LTS 362 >ref|NP_469752.1| low temperature requirement protein A [Listeria innocua] pir||AH1483 low temperature requirement protein A [imported] - Listeria innocua (strain Clip11262) emb|CAC95640.1| low temperature requirement protein A [Listeria innocua] Length = 371 Score = 252 bits (644), Expect = 8e-66, Method: Compositional matrix adjust. Identities = 131/315 (41%), Positives = 197/315 (62%), Gaps = 1/315 (0%) Query: 1 MTVKKVTWLELFYDLLYVAAVSKASHVLLHVEDETLSLET-LGKFVLIFIPIWWAWVGQT 59 MT +KV+WLELF+DL++V AV+ +H+LL V++ G+++L+ P++WAWVGQT Sbjct: 1 MTERKVSWLELFFDLIFVTAVASTTHLLLSVDNHPDKTAVYFGEYLLMVTPMFWAWVGQT 60 Query: 60 LFVNRYGQDIVSHRXXXXXXXXXXXXMTASLSVDFDQTYYSFLTGYLGLRAMTAVQYHYV 119 +F NR+G+ I MTAS + F TYY+FL GYLG+R +T +QY + Sbjct: 61 MFFNRFGEKIKLPELYMLPQMFFLILMTASFDLTFSNTYYTFLIGYLGIRFITVIQYFVM 120 Query: 120 RSKENGHLRATAHYLGSRFWIGLVISACSLFFDSWVRYAVLYAGIAVDIILPLAGRKILV 179 + G+ R A LGS F +G+ +A S+FFD ++RY V+Y GIAVDIILPL K L Sbjct: 121 SRQLVGNARKVAIILGSVFLLGVFTTATSVFFDGFIRYLVMYLGIAVDIILPLFLAKTLR 180 Query: 180 RTPANTHHLLERFSLFALILLGESVVSILAVLQSSTWTLQSVGFAGLTFVLIIAMWWQYY 239 + P + HL ERF LF +I GES+V+I +L T L ++ + L F++I ++ Y+ Sbjct: 181 KVPVDFPHLAERFGLFVIITFGESIVAITTILVGHTLDLYTISYTFLGFLIISTLFVSYF 240 Query: 240 DNLEKKVDKNVHTAGQTIIYGHLFIFLSLCMLAASIQLLFLNKLHYELMVNLLFGSVLLY 299 + EK +D + T GQ +IYGH FI +S+ +LAA++ LLF + L +++ +LFGSV L+ Sbjct: 241 HSFEKIIDHHKETHGQYLIYGHFFIIISIMLLAANLHLLFEDHLEKNVLLLMLFGSVALF 300 Query: 300 FASTTLVFHVYRHKQ 314 F S VF ++ + Sbjct: 301 FISKQFVFITHKKVE 315 >ref|NP_463919.1| low temperature requirement protein A [Listeria monocytogenes EGD-e] pir||AF1123 low temperature requirement protein A [imported] - Listeria monocytogenes (strain EGD-e) emb|CAC98468.1| low temperature requirement protein A [Listeria monocytogenes] Length = 371 Score = 243 bits (619), Expect = 8e-63, Method: Compositional matrix adjust. Identities = 131/317 (41%), Positives = 198/317 (62%), Gaps = 5/317 (1%) Query: 1 MTVKKVTWLELFYDLLYVAAVSKASHVLLHVE---DETLSLETLGKFVLIFIPIWWAWVG 57 MT +KV+WLELF+DL++V AV+ +H+LL V+ D+T + G+++L+ P++WAWVG Sbjct: 1 MTERKVSWLELFFDLIFVTAVASTTHLLLSVDNHPDKTAAY--FGEYLLMVTPMFWAWVG 58 Query: 58 QTLFVNRYGQDIVSHRXXXXXXXXXXXXMTASLSVDFDQTYYSFLTGYLGLRAMTAVQYH 117 QT+F NR+G+ I MTAS + F TYY+FL GYLG+R +T +QY Sbjct: 59 QTMFFNRFGEKIKWPELYMLSQMFFLILMTASFDLTFSNTYYTFLIGYLGIRFITVIQYF 118 Query: 118 YVRSKENGHLRATAHYLGSRFWIGLVISACSLFFDSWVRYAVLYAGIAVDIILPLAGRKI 177 + + G+ + A LGS F +G++ +A S+FF+ + RY V+Y GIAVDIILPL K Sbjct: 119 VISKQLTGNAQKVALLLGSVFLLGVLTTATSVFFEGFARYLVMYLGIAVDIILPLFLAKT 178 Query: 178 LVRTPANTHHLLERFSLFALILLGESVVSILAVLQSSTWTLQSVGFAGLTFVLIIAMWWQ 237 L + P + HL ERF LF +I GES+V+I +L T L ++ + L F++I +W Sbjct: 179 LRKVPVDFPHLAERFGLFVIITFGESIVAITTILVGHTLDLYTICYTLLGFLIICTLWAS 238 Query: 238 YYDNLEKKVDKNVHTAGQTIIYGHLFIFLSLCMLAASIQLLFLNKLHYELMVNLLFGSVL 297 Y+ + EK VD + T GQ +IYGH FI +S+ +LA ++ LLF L ++++ LLF SV Sbjct: 239 YFYSFEKIVDHHKVTHGQFLIYGHFFIIVSVMLLAVNLHLLFEGHLQRDVLLLLLFSSVA 298 Query: 298 LYFASTTLVFHVYRHKQ 314 ++F S VF ++ + Sbjct: 299 VFFISKQFVFAAHKKAE 315 >gb|AAC98899.1| low temperature requirement A protein [Listeria monocytogenes] Length = 324 Score = 219 bits (559), Expect = 5e-56, Method: Compositional matrix adjust. Identities = 111/265 (41%), Positives = 162/265 (61%) Query: 50 PIWWAWVGQTLFVNRYGQDIVSHRXXXXXXXXXXXXMTASLSVDFDQTYYSFLTGYLGLR 109 P++WAWVGQT+F NR+G+ I MTAS + F TYY+FL GYLG+R Sbjct: 4 PMFWAWVGQTMFFNRFGEKIKLPELYMLPQMFFLILMTASFDLTFSNTYYTFLIGYLGIR 63 Query: 110 AMTAVQYHYVRSKENGHLRATAHYLGSRFWIGLVISACSLFFDSWVRYAVLYAGIAVDII 169 +T +QY + + G+ R A LGS F +G++ +A S+FF+ + RY V+Y GIAVDII Sbjct: 64 LITVIQYFVISKQLTGNARKVAILLGSVFLLGVLTTATSVFFEGFARYLVMYLGIAVDII 123 Query: 170 LPLAGRKILVRTPANTHHLLERFSLFALILLGESVVSILAVLQSSTWTLQSVGFAGLTFV 229 LPL K L + P + HL ERF LF +I GES+V+I +L T ++G+ L F+ Sbjct: 124 LPLFLAKTLRKVPVDFPHLAERFGLFVIITFGESIVAITTILVGHTLDPYTIGYTLLGFL 183 Query: 230 LIIAMWWQYYDNLEKKVDKNVHTAGQTIIYGHLFIFLSLCMLAASIQLLFLNKLHYELMV 289 +I +W Y+ + EK VD + T GQ +IYGH FI +S+ +LAA++ LLF L +++ Sbjct: 184 IICTLWASYFHSFEKIVDHHKETHGQYLIYGHFFIIISVMLLAANVHLLFEGHLERNVLL 243 Query: 290 NLLFGSVLLYFASTTLVFHVYRHKQ 314 +LFGSV ++F S VF ++ + Sbjct: 244 LMLFGSVAVFFVSKQFVFAAHKKAE 268 >ref|ZP_00111390.1| COG4292: Predicted membrane protein [Nostoc punctiforme] Length = 393 Score = 143 bits (360), Expect = 7e-33, Method: Compositional matrix adjust. Identities = 95/307 (30%), Positives = 151/307 (49%), Gaps = 21/307 (6%) Query: 4 KKVTWLELFYDLLYVAAVSKASHVLLHVEDETLSLETLGKFVLIFIPIWWAWVGQTLFVN 63 + TWLELFYDL++V AVS+ +H L +E +SL L FV++FIP+WW+W+G T + N Sbjct: 20 RHATWLELFYDLVFVVAVSQLAHNL----NEDISLSGLFGFVVLFIPVWWSWIGTTFYAN 75 Query: 64 RYGQDIVSHRXXXXXXXXXXXXMTASLSVDFDQTYYSFLTGYLGLRAMTAVQYHYVRSKE 123 R+ D V HR M ++ ++ F Y RA+ ++Y VR+ Sbjct: 76 RFDSDDVGHRLLIGIQMLTAAAMAINIHHGLGESSPGFAISYALGRAVLVIEY--VRA-- 131 Query: 124 NGHLRATAHYLGSRFWIGLVISACSLFFDSWV----RYAVLYAGIAVDIILPLAGRKILV 179 G +A L +R+ IG I+A + V R+ GI +D PL G K V Sbjct: 132 -GRHIPSARPLTTRYAIGFAIAALLWLISASVPIPWRFGFWTLGIIIDFATPLKGHKFQV 190 Query: 180 RTPANTHHLLERFSLFALILLGESVVSILAVLQSSTWTLQSVGFAGLTFVLIIAMWWQYY 239 + HL ERF LF +I+LGE++++++ + W +V A ++ + WW Y+ Sbjct: 191 GLLPHASHLPERFGLFTIIVLGEAIIAVVNGVSEQKWDTLTVISAIFGLIIAFSWWWVYF 250 Query: 240 DNLEKKVDKNVHTAGQTII-----YGHLFIFLSLCMLAASIQLLFLNKLHYELMVN---L 291 DNL K + T G+ + Y HL + + + ++ + L+K L + L Sbjct: 251 DNLGGKPIETARTEGKVGVVNLWLYTHLPLVIGITAAGVGVEQILLSKATLALPDSQRWL 310 Query: 292 LFGSVLL 298 + GSV L Sbjct: 311 ICGSVAL 317 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.330 0.141 0.438 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 465,067,064 Number of extensions: 17007688 Number of successful extensions: 49903 Number of sequences better than 1.0e-05: 23 Number of HSP's gapped: 49866 Number of HSP's successfully gapped: 23 Length of query: 363 Length of database: 757,237,578 Length adjustment: 127 Effective length of query: 236 Effective length of database: 412,420,005 Effective search space: 97331121180 Effective search space used: 97331121180 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.9 bits) S2: 126 (53.1 bits)