BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0584.0027.p (323 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value dbj|BAA01729.1| cephalosporin-C deacetylase [Bacillus subtilis] 157 4e-37 ref|NP_388200.1| cephalosporin C deacetylase [Bacillus subtilis]... 155 1e-36 pdb|1ODT|C Chain C, Cephalosporin C Deacetylase Mutated, In Comp... 155 1e-36 pdb|1ODS|A Chain A, Cephalosporin C Deacetylase From Bacillus Su... 154 2e-36 pdb|1L7A|A Chain A, Structural Genomics, Crystal Structure Of Ce... 150 3e-35 >dbj|BAA01729.1| cephalosporin-C deacetylase [Bacillus subtilis] Length = 318 Score = 157 bits (396), Expect = 4e-37, Method: Compositional matrix adjust. Identities = 99/322 (30%), Positives = 156/322 (48%), Gaps = 10/322 (3%) Query: 1 MNTIELRKQELENCRPEPTLEQGVVNEFWDGILAEYARKPLEIISVPEVTPYPGMRVDKV 60 M +L +L+ +PE T + +EFW L E A+ E P P G++V ++ Sbjct: 1 MQLFDLPLDQLQTYKPEKTAPKDF-SEFWKLSLEELAKVQAEPDLQPVDYPADGVKVYRL 59 Query: 61 SFQSFDETRVHAWHIQPAQAGNGGGPLPCIITFPGYTGD-RGYPERYASWLLLGYSVLAV 119 +++SF R+ W+ P + GP P I+ + GY G +W L GY+ + Sbjct: 60 TYKSFGNARITGWYAVP----DKQGPHPAIVKYHGYNASYDGEIHEMVNWALHGYAAFGM 115 Query: 120 DVRGQLGETGNLLPMESGVAKGWITQGLLEKEHSYYMAVAMDTVRAVETAAQLQGVDPSR 179 VRGQ + + G A GW+T+G+L+K+ YY V +D VRA+E + VD +R Sbjct: 116 LVRGQQSSEDTSISLH-GHALGWMTKGILDKDTYYYRGVYLDAVRALEVISSFDEVDETR 174 Query: 180 XXXXXXXXXXXXXXXXXXXXXXXXXVAADIPNLCRLDFGV-LNSTSSLTEIGAYVRR--Y 236 AD P L + + + EI ++ RR Sbjct: 175 IGVTGGSQGGGLTIAAAALSDIPKAAVADYPYLSNFERAIDVALEQPYLEINSFFRRNGS 234 Query: 237 PEHLERVLDTLAYFDIVNLAPRITAPVLISVGWKDTVCMPESVYAAYNRIESDKQIMDYP 296 PE + + TL+YFDI+NLA R+ PVL+S+G D V P +V+AAYN +E++K++ Y Sbjct: 235 PETEVQAMKTLSYFDIMNLADRVKVPVLMSIGLIDKVTPPSTVFAAYNHLETEKELKVYR 294 Query: 297 FSGHEVSEYHLRQKVLFLQKHL 318 + GHE +K+ F ++HL Sbjct: 295 YFGHEYIPAFQTEKLAFFKQHL 316 >ref|NP_388200.1| cephalosporin C deacetylase [Bacillus subtilis] pir||G69596 cephalosporin-C deacetylase (EC 3.1.1.41) - Bacillus subtilis dbj|BAA08952.1| cephalosporin C deacetylase [Bacillus subtilis] emb|CAB12112.1| cephalosporin C deacetylase [Bacillus subtilis subsp. subtilis str. 168] Length = 318 Score = 155 bits (392), Expect = 1e-36, Method: Compositional matrix adjust. Identities = 101/323 (31%), Positives = 157/323 (48%), Gaps = 12/323 (3%) Query: 1 MNTIELRKQELENCRPEPTLEQGVVNEFWDGILAEYARKPLEIISVPEVTPYPGMRVDKV 60 M +L +L+ +PE T + +EFW L E A+ E P P G++V ++ Sbjct: 1 MQLFDLPLDQLQTYKPEKTAPKDF-SEFWKLSLEELAKVQAEPDLQPVDYPADGVKVYRL 59 Query: 61 SFQSFDETRVHAWHIQPAQAGNGGGPLPCIITFPGYTGD-RGYPERYASWLLLGYSVLAV 119 +++SF R+ W+ P + GP P I+ + GY G +W L GY+ + Sbjct: 60 TYKSFGNARITGWYAVP----DKEGPHPAIVKYHGYNASYDGEIHEMVNWALHGYATFGM 115 Query: 120 DVRGQLG-ETGNLLPMESGVAKGWITQGLLEKEHSYYMAVAMDTVRAVETAAQLQGVDPS 178 VRGQ E ++ P G A GW+T+G+L+K+ YY V +D VRA+E + VD + Sbjct: 116 LVRGQQSSEDTSISP--HGHALGWMTKGILDKDTYYYRGVYLDAVRALEVISSFDEVDET 173 Query: 179 RXXXXXXXXXXXXXXXXXXXXXXXXXVAADIPNLCRLDFGV-LNSTSSLTEIGAYVRR-- 235 R AD P L + + + EI ++ RR Sbjct: 174 RIGVTGGSQGGGLTIAAAALSDIPKAAVADYPYLSNFERAIDVALEQPYLEINSFFRRNG 233 Query: 236 YPEHLERVLDTLAYFDIVNLAPRITAPVLISVGWKDTVCMPESVYAAYNRIESDKQIMDY 295 PE + + TL+YFDI+NLA R+ PVL+S+G D V P +V+AAYN +E+ K++ Y Sbjct: 234 SPETEVQAMKTLSYFDIMNLADRVKVPVLMSIGLIDKVTPPSTVFAAYNHLETKKELKVY 293 Query: 296 PFSGHEVSEYHLRQKVLFLQKHL 318 + GHE +K+ F ++HL Sbjct: 294 RYFGHEYIPAFQTEKLAFFKQHL 316 >pdb|1ODT|C Chain C, Cephalosporin C Deacetylase Mutated, In Complex With Acetate pdb|1ODT|H Chain H, Cephalosporin C Deacetylase Mutated, In Complex With Acetate Length = 318 Score = 155 bits (391), Expect = 1e-36, Method: Compositional matrix adjust. Identities = 101/323 (31%), Positives = 157/323 (48%), Gaps = 12/323 (3%) Query: 1 MNTIELRKQELENCRPEPTLEQGVVNEFWDGILAEYARKPLEIISVPEVTPYPGMRVDKV 60 M +L +L+ +PE T + +EFW L E A+ E P P G++V ++ Sbjct: 1 MQLFDLPLDQLQTYKPEKTAPKDF-SEFWKLSLEELAKVQAEPDLQPVDYPADGVKVYRL 59 Query: 61 SFQSFDETRVHAWHIQPAQAGNGGGPLPCIITFPGYTGD-RGYPERYASWLLLGYSVLAV 119 +++SF R+ W+ P + GP P I+ + GY G +W L GY+ + Sbjct: 60 TYKSFGNARITGWYAVP----DKEGPHPAIVKYHGYNASYDGEIHEMVNWALHGYATFGM 115 Query: 120 DVRGQLG-ETGNLLPMESGVAKGWITQGLLEKEHSYYMAVAMDTVRAVETAAQLQGVDPS 178 VRGQ E ++ P G A GW+T+G+L+K+ YY V +D VRA+E + VD + Sbjct: 116 LVRGQQSSEDTSISP--HGHALGWMTKGILDKDTYYYRGVYLDAVRALEVISSFDEVDET 173 Query: 179 RXXXXXXXXXXXXXXXXXXXXXXXXXVAADIPNLCRLDFGV-LNSTSSLTEIGAYVRR-- 235 R AD P L + + + EI ++ RR Sbjct: 174 RIGVTGGAQGGGLTIAAAALSDIPKAAVADYPYLSNFERAIDVALEEPYLEINSFFRRNG 233 Query: 236 YPEHLERVLDTLAYFDIVNLAPRITAPVLISVGWKDTVCMPESVYAAYNRIESDKQIMDY 295 PE + + TL+YFDI+NLA R+ PVL+S+G D V P +V+AAYN +E+ K++ Y Sbjct: 234 SPETEVQAMKTLSYFDIMNLADRVKVPVLMSIGLIDKVTPPSTVFAAYNHLETKKELKVY 293 Query: 296 PFSGHEVSEYHLRQKVLFLQKHL 318 + GHE +K+ F ++HL Sbjct: 294 RYFGHEYIPAFQTEKLAFFKQHL 316 >pdb|1ODS|A Chain A, Cephalosporin C Deacetylase From Bacillus Subtilis pdb|1ODS|B Chain B, Cephalosporin C Deacetylase From Bacillus Subtilis pdb|1ODS|C Chain C, Cephalosporin C Deacetylase From Bacillus Subtilis pdb|1ODS|D Chain D, Cephalosporin C Deacetylase From Bacillus Subtilis pdb|1ODS|E Chain E, Cephalosporin C Deacetylase From Bacillus Subtilis pdb|1ODS|F Chain F, Cephalosporin C Deacetylase From Bacillus Subtilis pdb|1ODS|G Chain G, Cephalosporin C Deacetylase From Bacillus Subtilis pdb|1ODS|H Chain H, Cephalosporin C Deacetylase From Bacillus Subtilis Length = 318 Score = 154 bits (390), Expect = 2e-36, Method: Compositional matrix adjust. Identities = 101/323 (31%), Positives = 157/323 (48%), Gaps = 12/323 (3%) Query: 1 MNTIELRKQELENCRPEPTLEQGVVNEFWDGILAEYARKPLEIISVPEVTPYPGMRVDKV 60 M +L +L+ +PE T + +EFW L E A+ E P P G++V ++ Sbjct: 1 MQLFDLPLDQLQTYKPEKTAPKDF-SEFWKLSLEELAKVQAEPDLQPVDYPADGVKVYRL 59 Query: 61 SFQSFDETRVHAWHIQPAQAGNGGGPLPCIITFPGYTGD-RGYPERYASWLLLGYSVLAV 119 +++SF R+ W+ P + GP P I+ + GY G +W L GY+ + Sbjct: 60 TYKSFGNARITGWYAVP----DKEGPHPAIVKYHGYNASYDGEIHEMVNWALHGYATFGM 115 Query: 120 DVRGQLG-ETGNLLPMESGVAKGWITQGLLEKEHSYYMAVAMDTVRAVETAAQLQGVDPS 178 VRGQ E ++ P G A GW+T+G+L+K+ YY V +D VRA+E + VD + Sbjct: 116 LVRGQQSSEDTSISP--HGHALGWMTKGILDKDTYYYRGVYLDAVRALEVISSFDEVDET 173 Query: 179 RXXXXXXXXXXXXXXXXXXXXXXXXXVAADIPNLCRLDFGV-LNSTSSLTEIGAYVRR-- 235 R AD P L + + + EI ++ RR Sbjct: 174 RIGVTGGSQGGGLTIAAAALSDIPKAAVADYPYLSNFERAIDVALEEPYLEINSFFRRNG 233 Query: 236 YPEHLERVLDTLAYFDIVNLAPRITAPVLISVGWKDTVCMPESVYAAYNRIESDKQIMDY 295 PE + + TL+YFDI+NLA R+ PVL+S+G D V P +V+AAYN +E+ K++ Y Sbjct: 234 SPETEVQAMKTLSYFDIMNLAYRVKVPVLMSIGLIDKVTPPSTVFAAYNHLETKKELKVY 293 Query: 296 PFSGHEVSEYHLRQKVLFLQKHL 318 + GHE +K+ F ++HL Sbjct: 294 RYFGHEYIPAFQTEKLAFFKQHL 316 >pdb|1L7A|A Chain A, Structural Genomics, Crystal Structure Of Cephalosporin C Deacetylase pdb|1L7A|B Chain B, Structural Genomics, Crystal Structure Of Cephalosporin C Deacetylase Length = 318 Score = 150 bits (380), Expect = 3e-35, Method: Compositional matrix adjust. Identities = 100/323 (30%), Positives = 156/323 (48%), Gaps = 12/323 (3%) Query: 1 MNTIELRKQELENCRPEPTLEQGVVNEFWDGILAEYARKPLEIISVPEVTPYPGMRVDKV 60 M +L +L+ +PE T + +EFW L E A+ E P P G++V ++ Sbjct: 1 MQLFDLPLDQLQTYKPEKTAPKDF-SEFWKLSLEELAKVQAEPDLQPVDYPADGVKVYRL 59 Query: 61 SFQSFDETRVHAWHIQPAQAGNGGGPLPCIITFPGYTGD-RGYPERYASWLLLGYSVLAV 119 +++SF R+ W+ P + GP P I+ + GY G +W L GY+ + Sbjct: 60 TYKSFGNARITGWYAVP----DKEGPHPAIVKYHGYNASYDGEIHEMVNWALHGYATFGM 115 Query: 120 DVRGQ-LGETGNLLPMESGVAKGWITQGLLEKEHSYYMAVAMDTVRAVETAAQLQGVDPS 178 VRGQ E ++ P G A GW+T+G+L+K+ YY V +D VRA+E + VD + Sbjct: 116 LVRGQQRSEDTSISP--HGHALGWMTKGILDKDTYYYRGVYLDAVRALEVISSFDEVDET 173 Query: 179 RXXXXXXXXXXXXXXXXXXXXXXXXXVAADIPNLCRLDFGV-LNSTSSLTEIGAYVRR-- 235 R AD P L + + + EI ++ RR Sbjct: 174 RIGVTGGSQGGGLTIAAAALSDIPKAAVADYPYLSNFERAIDVALEQPYLEINSFFRRNG 233 Query: 236 YPEHLERVLDTLAYFDIVNLAPRITAPVLISVGWKDTVCMPESVYAAYNRIESDKQIMDY 295 PE + + TL+YFDI+NLA R+ PVL+S+G D V P +V+AAYN +E+ K++ Y Sbjct: 234 SPETEVQAMKTLSYFDIMNLADRVKVPVLMSIGLIDKVTPPSTVFAAYNHLETKKELKVY 293 Query: 296 PFSGHEVSEYHLRQKVLFLQKHL 318 + GHE +K+ F ++ L Sbjct: 294 RYFGHEYIPAFQTEKLAFFKQIL 316 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.319 0.137 0.417 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 468,765,476 Number of extensions: 19563177 Number of successful extensions: 47697 Number of sequences better than 1.0e-05: 33 Number of HSP's gapped: 47643 Number of HSP's successfully gapped: 33 Length of query: 323 Length of database: 757,237,578 Length adjustment: 126 Effective length of query: 197 Effective length of database: 415,135,104 Effective search space: 81781615488 Effective search space used: 81781615488 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 126 (53.1 bits)