BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0584.0014.p (197 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_616145.1| antibiotic resistance protein [Methanosarcina a... 206 2e-52 ref|NP_241443.1| BH0577~unknown conserved protein in others [Bac... 154 1e-36 ref|NP_907745.1| HYPOTHETICAL PROTEIN-Predicted flavin-nucleotid... 83 3e-15 pir||I40599 5-Nitroimidazole antibiotics resistance protein - Ba... 78 9e-14 emb|CAD56147.1| nitroimidazole resistance protein [Bacteroides v... 76 4e-13 >ref|NP_616145.1| antibiotic resistance protein [Methanosarcina acetivorans str. C2A] gb|AAM04625.1| antibiotic resistance protein [Methanosarcina acetivorans str. C2A] Length = 177 Score = 206 bits (524), Expect = 2e-52, Method: Compositional matrix adjust. Identities = 92/165 (55%), Positives = 122/165 (73%) Query: 31 LEHVSYKLRECQDQERIGAFLSVSRTGIVGIGGDTYPYAVPVNYVWHQGSIYFHGMGSGK 90 ++ V Y R C D+E+I +FL RTG++G+ +PYAVP+NYVWH+GS+Y HGMGSGK Sbjct: 1 MDQVRYTQRNCTDKEKIESFLLQERTGVLGMVNGIFPYAVPMNYVWHKGSVYLHGMGSGK 60 Query: 91 KVRLLAEHPAVSFTVYREDCTVTDPVPCKADTAYFSVMLFGEAIRVTDMGEAAGVLQKIL 150 K +L+ P V FT+Y+E TVTDPVPC ADTAY SVM+FG+A +V+D EAA L K++ Sbjct: 61 KEEILSRSPPVCFTIYKEHGTVTDPVPCHADTAYMSVMIFGKAEKVSDQEEAAEALWKLV 120 Query: 151 DKYTPGFYKQELSARMVEKYRSSMDGNGAAVYRLTPVHLTAKENA 195 +KY PG+Y L+ VEKYRSS+D N +VYR+TP +TAKEN+ Sbjct: 121 NKYMPGYYNNPLTGTFVEKYRSSLDENPVSVYRITPQWMTAKENS 165 >ref|NP_241443.1| BH0577~unknown conserved protein in others [Bacillus halodurans] pir||A83722 hypothetical protein BH0577 [imported] - Bacillus halodurans (strain C-125) dbj|BAB04296.1| BH0577~unknown conserved protein in others [Bacillus halodurans] Length = 184 Score = 154 bits (388), Expect = 1e-36, Method: Compositional matrix adjust. Identities = 68/167 (40%), Positives = 108/167 (64%), Gaps = 1/167 (0%) Query: 31 LEHVSYKLRECQDQERIGAFLSVSRTGIVGIGGDTYPYAVPVNYVWHQGSIYFHGMGSGK 90 + + Y REC+D+++I FL+ +RTG +G+ + PY +P+N+VWH +IYFHG G+ Sbjct: 1 MNAIRYTKRECKDEKKITEFLNKARTGFLGLSTNDQPYVIPLNFVWHNHAIYFHGASEGR 60 Query: 91 KVRLLAEHPAVSFTVYREDCTVTDPVPCKADTAYFSVMLFGEAIRVTDMGEAAGVLQKIL 150 K++++ +P V FT+ + T+ PVP DTAY SV++FG V+ + E +Q++L Sbjct: 61 KIKMIEANPEVCFTICEDLGTIVSPVPAHTDTAYMSVIIFGTIEPVSAIEEGTEAMQQML 120 Query: 151 DKYTPGFYKQELSARMVEKYRSSMDGNGAAVYRLTPVHLTAKENAAV 197 DKY PG+Y L+A VEKYRSS+ G+ A+Y+++ TAK N + Sbjct: 121 DKYVPGYYHSPLAASHVEKYRSSL-GSRTAIYKISCRERTAKVNEPI 166 >ref|NP_907745.1| HYPOTHETICAL PROTEIN-Predicted flavin-nucleotide-binding [Wolinella succinogenes] emb|CAE10645.1| HYPOTHETICAL PROTEIN-Predicted flavin-nucleotide-binding [Wolinella succinogenes] Length = 191 Score = 83.2 bits (204), Expect = 3e-15, Method: Compositional matrix adjust. Identities = 45/152 (29%), Positives = 84/152 (55%), Gaps = 12/152 (7%) Query: 44 QERIGAFLSVSRTGIVGI-GGDTYPYAVPVNYVWHQGSIYFHGMGSGKKVRLLAEHPAVS 102 +E I L++++ G +GI D +PY +P+++V+ IY HG+ GKK+ + ++P V Sbjct: 46 KEEIKEALNLAQVGRIGIVCSDGFPYVLPLHFVYADEKIYLHGLPKGKKIEGIKQNPNVC 105 Query: 103 FTVYREDCTVTDPV--PCKADTAYFSVMLFGEAIRVTDMGEAAGVLQKILDKYTPGFYKQ 160 F + + + + V PC +T + SV+L G+A V D E L++I++K+TP Q Sbjct: 106 FEIDQMVSLLKEGVENPCDVNTEFNSVILQGKASLVGDFEEKKSALKRIVEKFTPLLINQ 165 Query: 161 ELSARMVEKYRSSMDGNGAAVYRLTPVHLTAK 192 E+ +M++ G A+ R+ ++ T + Sbjct: 166 EIPDKMIQ---------GTAIIRIDILNSTGR 188 >pir||I40599 5-Nitroimidazole antibiotics resistance protein - Bacteroides vulgatus emb|CAA50581.1| nimA [Bacteroides vulgatus] Length = 176 Score = 78.2 bits (191), Expect = 9e-14, Method: Compositional matrix adjust. Identities = 46/119 (38%), Positives = 65/119 (54%), Gaps = 5/119 (4%) Query: 39 RECQDQERIGAFLSVSRTGIVGIGGDT-YPYAVPVNYVWHQGSIYFHGMGSGKKVRLLAE 97 R+ E A L G + + GD YPYAVPV+YV+ G IYFHG G K+ + + Sbjct: 9 RQLLPPEESLAILERMTGGTLALHGDNGYPYAVPVSYVYADGKIYFHGAVQGHKMDAIRQ 68 Query: 98 HPAVSFTVYREDCTVTDPVPCKADTAYFSVMLFGEAIRVTDMGEAAGVLQKILDKYTPG 156 HP VSF V +D VP + T + SV++FG+A +TD E L ++ +KY+ G Sbjct: 69 HPEVSFCVVEQDRI----VPAEFTTYFRSVIVFGKARILTDEVEKRAALLRLAEKYSSG 123 >emb|CAD56147.1| nitroimidazole resistance protein [Bacteroides vulgatus] Length = 152 Score = 76.3 bits (186), Expect = 4e-13, Method: Compositional matrix adjust. Identities = 47/128 (36%), Positives = 66/128 (51%), Gaps = 6/128 (4%) Query: 30 MLEHVSYKLRECQDQERIGAFLSVSRTGIVGIGGDT-YPYAVPVNYVWHQGSIYFHGMGS 88 M + K ++ +E I A L G++ + GD YPYAVPV+YV+ G IYFH Sbjct: 1 MFREMRRKRQQLSAEESI-AILERMTNGVLALHGDEGYPYAVPVSYVYADGKIYFHSAMK 59 Query: 89 GKKVRLLAEHPAVSFTVYREDCTVTDPVPCKADTAYFSVMLFGEAIRVTDMGEAAGVLQK 148 G KV + + VSF V D D P + T + SV+LFG+ +T+ E L Sbjct: 60 GHKVDAIMRNERVSFCVVERD----DVCPGEFTTYFRSVVLFGKTRILTEEAEKLAALSL 115 Query: 149 ILDKYTPG 156 + DKY+PG Sbjct: 116 LADKYSPG 123 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.320 0.136 0.408 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 302,614,955 Number of extensions: 12346559 Number of successful extensions: 24022 Number of sequences better than 1.0e-05: 22 Number of HSP's gapped: 24019 Number of HSP's successfully gapped: 22 Length of query: 197 Length of database: 757,237,578 Length adjustment: 119 Effective length of query: 78 Effective length of database: 434,140,797 Effective search space: 33862982166 Effective search space used: 33862982166 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 122 (51.6 bits)