BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0584.0012.p (462 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_00015418.1| COG2710: Nitrogenase molybdenum-iron protein,... 760 0.0 ref|ZP_00077948.1| COG2710: Nitrogenase molybdenum-iron protein,... 753 0.0 ref|NP_616147.1| nitrogenase, subunit K [Methanosarcina acetivor... 744 0.0 ref|ZP_00092172.1| COG2710: Nitrogenase molybdenum-iron protein,... 740 0.0 ref|NP_946785.1| alternative nitrogenase 3 beta chain [Rhodopseu... 727 0.0 >ref|ZP_00015418.1| COG2710: Nitrogenase molybdenum-iron protein, alpha and beta chains [Rhodospirillum rubrum] Length = 462 Score = 760 bits (1962), Expect = 0.0, Method: Compositional matrix adjust. Identities = 364/462 (78%), Positives = 408/462 (88%) Query: 1 MTCEIVQKERGGVINPIFTCQPAGAQYASIGIKDCIGIVHGGQGCVMFVRLLFSQHFKDN 60 M+CE+ +KER G INPIFTCQPAGAQYASIGIKDCIGIVHGGQGCVMFVRLL SQH K++ Sbjct: 1 MSCELRKKERIGTINPIFTCQPAGAQYASIGIKDCIGIVHGGQGCVMFVRLLISQHLKES 60 Query: 61 FLIASSSVHEDGAVFGALNRVEEAVDVLLMRYPDVKVVPIITTCSTEVIGDDVDGVILKL 120 F IASSSVHEDGAVFGAL+RVE+AVDVLLMRYPDVKV+PIITTCSTEVIGDDVDGV+ KL Sbjct: 61 FEIASSSVHEDGAVFGALDRVEQAVDVLLMRYPDVKVIPIITTCSTEVIGDDVDGVVRKL 120 Query: 121 NNGLLKEKYAGREVHLIAVHTPSFVGSQVSGYDVAVEAFVKHFARKGQPNGKLNLITGWV 180 GLLKEK+ REVHLI +HTPSFVGS V+GYDVAV FVK FA+KG+P+ K+NLITGWV Sbjct: 121 EEGLLKEKFPDREVHLIPIHTPSFVGSMVTGYDVAVRDFVKAFAKKGKPSLKINLITGWV 180 Query: 181 NPGDVTALKHLLAEMQVDATVLFEIESFDSPILPDKSGESHGSTTIADMTGXXXXXXXXX 240 NPGDV LKHLLAEM+V+A VLFEIESFDSP++PDKS SHGSTTI D+ Sbjct: 181 NPGDVKELKHLLAEMRVEANVLFEIESFDSPLMPDKSAVSHGSTTIDDLIDTANAQGTIA 240 Query: 241 XXKYEGAKAAKYLEDEFGIPAVIGPTPIGIRNTDTFLQNVRKLTGKPIPESLVRERGIAI 300 +YEGAKAA++L+ F +PAVIGPTPIGIRNTDTFLQN++ +TGK IP SLVRERGIAI Sbjct: 241 LNRYEGAKAAEFLQKGFDVPAVIGPTPIGIRNTDTFLQNLKIMTGKAIPPSLVRERGIAI 300 Query: 301 DALADLVHMFLADKKVAIYGHPDLVIGLAEFCIDLEMQPVLLLLGDDNTTYKSDPRIQAL 360 DAL DLVHMFLADKKVAIYG+PDLVIGLAEFC+DLEM+P+LLLLGD+N Y++DPRI+AL Sbjct: 301 DALTDLVHMFLADKKVAIYGNPDLVIGLAEFCLDLEMKPMLLLLGDENAGYETDPRIKAL 360 Query: 361 QQNVEFGMEIVLNADLLELENRIKNKGLELDLILGHSKGRFTAIDNNIPMVRVGFPTYDR 420 Q+ V+ MEI+ NADL ELE+R+KNKGLELDLILGHSKGRFTAIDNNIPMVRVGFPTYDR Sbjct: 361 QERVDHDMEIITNADLWELEDRVKNKGLELDLILGHSKGRFTAIDNNIPMVRVGFPTYDR 420 Query: 421 AGLYRHPTVGYAGAIRLAEEIANTLFTDMEYKKNKEWILSVW 462 AGLYRHP VGYAGA LAE +ANTLFTDMEYKKNKEWIL+VW Sbjct: 421 AGLYRHPVVGYAGATWLAEAMANTLFTDMEYKKNKEWILNVW 462 >ref|ZP_00077948.1| COG2710: Nitrogenase molybdenum-iron protein, alpha and beta chains [Methanosarcina barkeri] Length = 462 Score = 753 bits (1944), Expect = 0.0, Method: Compositional matrix adjust. Identities = 359/462 (77%), Positives = 413/462 (89%) Query: 1 MTCEIVQKERGGVINPIFTCQPAGAQYASIGIKDCIGIVHGGQGCVMFVRLLFSQHFKDN 60 M+CE+ Q+ER G+INPIFTCQP GAQYAS+GIKDCI IVHGGQGCVMFVR+LF+QHFKD+ Sbjct: 1 MSCELKQRERPGIINPIFTCQPCGAQYASVGIKDCIAIVHGGQGCVMFVRMLFAQHFKDS 60 Query: 61 FLIASSSVHEDGAVFGALNRVEEAVDVLLMRYPDVKVVPIITTCSTEVIGDDVDGVILKL 120 F IASSS+HEDGAVFGALNRVEEAVDVLLMRYP VKV+PIITTCSTEVIGDD+DG+I KL Sbjct: 61 FEIASSSLHEDGAVFGALNRVEEAVDVLLMRYPHVKVIPIITTCSTEVIGDDIDGLIRKL 120 Query: 121 NNGLLKEKYAGREVHLIAVHTPSFVGSQVSGYDVAVEAFVKHFARKGQPNGKLNLITGWV 180 N LLKEKYA REVHLI +HTPSF GS ++GYDVAV VK FA+K +PNGKLNLITGWV Sbjct: 121 NKKLLKEKYANREVHLIPIHTPSFKGSMITGYDVAVHDIVKEFAKKDKPNGKLNLITGWV 180 Query: 181 NPGDVTALKHLLAEMQVDATVLFEIESFDSPILPDKSGESHGSTTIADMTGXXXXXXXXX 240 NPGDVTALKHLL+EM +DATVLFEIESFDSP++PDKSG +HGSTTI D+TG Sbjct: 181 NPGDVTALKHLLSEMDIDATVLFEIESFDSPLMPDKSGFAHGSTTIEDLTGTADALGTIA 240 Query: 241 XXKYEGAKAAKYLEDEFGIPAVIGPTPIGIRNTDTFLQNVRKLTGKPIPESLVRERGIAI 300 KYEG KAA+YL+++F +PA+IGPTPIGIRNTDTFLQN++K+TGKPIPESLVRERGIAI Sbjct: 241 LNKYEGGKAAEYLKNKFDVPAIIGPTPIGIRNTDTFLQNLKKMTGKPIPESLVRERGIAI 300 Query: 301 DALADLVHMFLADKKVAIYGHPDLVIGLAEFCIDLEMQPVLLLLGDDNTTYKSDPRIQAL 360 DA+ DL HMF ADKKVAIYG+PDLVIGLAEFC+D+EM+PV+LLLGDDN TYK DPRI+ Sbjct: 301 DAITDLTHMFFADKKVAIYGNPDLVIGLAEFCLDMEMKPVVLLLGDDNNTYKDDPRIKEF 360 Query: 361 QQNVEFGMEIVLNADLLELENRIKNKGLELDLILGHSKGRFTAIDNNIPMVRVGFPTYDR 420 Q+ V+F M+I++NADL ELE RIK+K +ELDLILGHSKGR+TAIDNNIPMVRVGFPT+DR Sbjct: 361 QEKVDFDMQIIMNADLWELERRIKDKEVELDLILGHSKGRWTAIDNNIPMVRVGFPTFDR 420 Query: 421 AGLYRHPTVGYAGAIRLAEEIANTLFTDMEYKKNKEWILSVW 462 AGLYRHP VGY GAI LAE++ANTLFTDME+KK++EWIL+VW Sbjct: 421 AGLYRHPVVGYEGAIWLAEQMANTLFTDMEFKKDREWILNVW 462 >ref|NP_616147.1| nitrogenase, subunit K [Methanosarcina acetivorans str. C2A] gb|AAM04627.1| nitrogenase, subunit K [Methanosarcina acetivorans str. C2A] Length = 462 Score = 744 bits (1922), Expect = 0.0, Method: Compositional matrix adjust. Identities = 356/462 (77%), Positives = 408/462 (88%) Query: 1 MTCEIVQKERGGVINPIFTCQPAGAQYASIGIKDCIGIVHGGQGCVMFVRLLFSQHFKDN 60 M+CE+ ++ER G+INPIFTCQP GAQYASIGIKDCI IVHGGQGCVMFVR+L +QHFKD+ Sbjct: 1 MSCELKERERPGIINPIFTCQPCGAQYASIGIKDCIAIVHGGQGCVMFVRMLLAQHFKDS 60 Query: 61 FLIASSSVHEDGAVFGALNRVEEAVDVLLMRYPDVKVVPIITTCSTEVIGDDVDGVILKL 120 F IASSS+HEDGAVFGALNRVEEAVDVLL RYP VKVVPIITTCSTEVIGDD+DG+I KL Sbjct: 61 FEIASSSLHEDGAVFGALNRVEEAVDVLLTRYPRVKVVPIITTCSTEVIGDDIDGLIRKL 120 Query: 121 NNGLLKEKYAGREVHLIAVHTPSFVGSQVSGYDVAVEAFVKHFARKGQPNGKLNLITGWV 180 N LLKEKYA REVHLI +HTPSF GS V+GYDVAV VK FA+K +PNGK+NLITGWV Sbjct: 121 NKKLLKEKYADREVHLIPIHTPSFKGSMVTGYDVAVHDIVKEFAKKDKPNGKINLITGWV 180 Query: 181 NPGDVTALKHLLAEMQVDATVLFEIESFDSPILPDKSGESHGSTTIADMTGXXXXXXXXX 240 NPGDVTALKHLL+ M +DATVLFEIESFDSP++PDKSG +HGSTTI D+TG Sbjct: 181 NPGDVTALKHLLSAMDIDATVLFEIESFDSPLMPDKSGVAHGSTTIEDLTGTANATGTIA 240 Query: 241 XXKYEGAKAAKYLEDEFGIPAVIGPTPIGIRNTDTFLQNVRKLTGKPIPESLVRERGIAI 300 +YEGAKAAKYLE EF +PA+IGPTPIGIRNTDTFLQN++K+TGKPIPESLV ERGIAI Sbjct: 241 LNRYEGAKAAKYLESEFDVPAIIGPTPIGIRNTDTFLQNLKKMTGKPIPESLVCERGIAI 300 Query: 301 DALADLVHMFLADKKVAIYGHPDLVIGLAEFCIDLEMQPVLLLLGDDNTTYKSDPRIQAL 360 DA+ DL HMF ADKKVAIYG+PDLVIGLAEFC+D+EM+P++LLLGDDN TY DPRI+ L Sbjct: 301 DAITDLTHMFFADKKVAIYGNPDLVIGLAEFCLDMEMKPMVLLLGDDNKTYPDDPRIKEL 360 Query: 361 QQNVEFGMEIVLNADLLELENRIKNKGLELDLILGHSKGRFTAIDNNIPMVRVGFPTYDR 420 Q+ V+F MEI++NAD+ ELE RIK+K +ELDLI+GHSKGR+TAIDNNIPMVRVGFPT+DR Sbjct: 361 QEKVDFDMEIIMNADMWELERRIKDKEIELDLIMGHSKGRWTAIDNNIPMVRVGFPTFDR 420 Query: 421 AGLYRHPTVGYAGAIRLAEEIANTLFTDMEYKKNKEWILSVW 462 AG+YRHP VGY GAI LAE++ANTLF DMEYKK++EWIL+VW Sbjct: 421 AGMYRHPVVGYEGAIWLAEQMANTLFADMEYKKDREWILNVW 462 >ref|ZP_00092172.1| COG2710: Nitrogenase molybdenum-iron protein, alpha and beta chains [Azotobacter vinelandii] sp|P16267|ANFK_AZOVI NITROGENASE IRON-IRON PROTEIN BETA CHAIN (NITROGENASE COMPONENT I) (DINITROGENASE 3 BETA SUBUNIT) pir||D32057 nitrogenase (EC 1.18.6.1) 3 beta chain - Azotobacter vinelandii gb|AAA82511.1| nitrogenase 3 subunit K Length = 462 Score = 740 bits (1910), Expect = 0.0, Method: Compositional matrix adjust. Identities = 350/462 (75%), Positives = 400/462 (86%) Query: 1 MTCEIVQKERGGVINPIFTCQPAGAQYASIGIKDCIGIVHGGQGCVMFVRLLFSQHFKDN 60 MTCE+ +K R G INPIFTCQPAGAQ+ SIGIKDCIGIVHGGQGCVMFVRL+FSQH+K++ Sbjct: 1 MTCEVKEKGRVGTINPIFTCQPAGAQFVSIGIKDCIGIVHGGQGCVMFVRLIFSQHYKES 60 Query: 61 FLIASSSVHEDGAVFGALNRVEEAVDVLLMRYPDVKVVPIITTCSTEVIGDDVDGVILKL 120 F +ASSS+HEDGAVFGA RVEEAVDVLL RYPDVKVVPIITTCSTE+IGDDVDGVI KL Sbjct: 61 FELASSSLHEDGAVFGACGRVEEAVDVLLSRYPDVKVVPIITTCSTEIIGDDVDGVIKKL 120 Query: 121 NNGLLKEKYAGREVHLIAVHTPSFVGSQVSGYDVAVEAFVKHFARKGQPNGKLNLITGWV 180 N GLLKEK+ REVHLIA+HTPSFVGS +SGYDVAV V+HFA++ PN K+NL+TGWV Sbjct: 121 NEGLLKEKFPDREVHLIAMHTPSFVGSMISGYDVAVRDVVRHFAKREAPNDKINLLTGWV 180 Query: 181 NPGDVTALKHLLAEMQVDATVLFEIESFDSPILPDKSGESHGSTTIADMTGXXXXXXXXX 240 NPGDV LKHLL EM ++A VLFEIESFDSPILPD S SHG+TTI D+ Sbjct: 181 NPGDVKELKHLLGEMDIEANVLFEIESFDSPILPDGSAVSHGNTTIEDLIDTGNARATFA 240 Query: 241 XXKYEGAKAAKYLEDEFGIPAVIGPTPIGIRNTDTFLQNVRKLTGKPIPESLVRERGIAI 300 +YEG KAA+YL+ +F IPA+IGPTPIGIRNTD FLQN++K TGKPIP+SL ERG+AI Sbjct: 241 LNRYEGTKAAEYLQKKFEIPAIIGPTPIGIRNTDIFLQNLKKATGKPIPQSLAHERGVAI 300 Query: 301 DALADLVHMFLADKKVAIYGHPDLVIGLAEFCIDLEMQPVLLLLGDDNTTYKSDPRIQAL 360 DALADL HMFLA+K+VAIYG PDLVIGLAEFC+DLEM+PVLLLLGDDN+ Y DPRI+AL Sbjct: 301 DALADLTHMFLAEKRVAIYGAPDLVIGLAEFCLDLEMKPVLLLLGDDNSKYVDDPRIKAL 360 Query: 361 QQNVEFGMEIVLNADLLELENRIKNKGLELDLILGHSKGRFTAIDNNIPMVRVGFPTYDR 420 Q+NV++GMEIV NAD ELENRIKN+GLELDLILGHSKGRF +ID NIPM+RVGFPTYDR Sbjct: 361 QENVDYGMEIVTNADFWELENRIKNEGLELDLILGHSKGRFISIDYNIPMLRVGFPTYDR 420 Query: 421 AGLYRHPTVGYAGAIRLAEEIANTLFTDMEYKKNKEWILSVW 462 AGL+R+PTVGY GAI LAE++ANTLF DME+KKNKEW+L+VW Sbjct: 421 AGLFRYPTVGYGGAIWLAEQMANTLFADMEHKKNKEWVLNVW 462 >ref|NP_946785.1| alternative nitrogenase 3 beta chain [Rhodopseudomonas palustris CGA009] emb|CAE26878.1| alternative nitrogenase 3 beta chain [Rhodopseudomonas palustris CGA009] Length = 461 Score = 727 bits (1877), Expect = 0.0, Method: Compositional matrix adjust. Identities = 349/462 (75%), Positives = 395/462 (85%), Gaps = 1/462 (0%) Query: 1 MTCEIVQKERGGVINPIFTCQPAGAQYASIGIKDCIGIVHGGQGCVMFVRLLFSQHFKDN 60 M C+ K+R G INPIFTCQPAGAQYASIGIKDCIGIVHGGQGCVMFVRLL SQH K++ Sbjct: 1 MNCQFKPKDRIGTINPIFTCQPAGAQYASIGIKDCIGIVHGGQGCVMFVRLLISQHLKES 60 Query: 61 FLIASSSVHEDGAVFGALNRVEEAVDVLLMRYPDVKVVPIITTCSTEVIGDDVDGVILKL 120 F IASSSVHEDGAVFGAL+RVEEAVDVLLMRYP VKVVPIITTCSTEVIGDDVDGV+ KL Sbjct: 61 FEIASSSVHEDGAVFGALDRVEEAVDVLLMRYPHVKVVPIITTCSTEVIGDDVDGVVTKL 120 Query: 121 NNGLLKEKYAGREVHLIAVHTPSFVGSQVSGYDVAVEAFVKHFARKGQPNGKLNLITGWV 180 LL KY REVHLI +H PSFVGS V+GYDVAV FVKHF++K +P+ K+NLITGWV Sbjct: 121 QEELLDVKYPDREVHLIPIHCPSFVGSMVTGYDVAVRDFVKHFSKKDKPSLKINLITGWV 180 Query: 181 NPGDVTALKHLLAEMQVDATVLFEIESFDSPILPDKSGESHGSTTIADMTGXXXXXXXXX 240 NPGDV LKHLLAEMQV+A VLFEIESFDSP++PDKS SHGSTTIAD+T Sbjct: 181 NPGDVKELKHLLAEMQVEANVLFEIESFDSPLMPDKSAVSHGSTTIADLTATADAQGTIA 240 Query: 241 XXKYEGAKAAKYLEDEFGIPAVIGPTPIGIRNTDTFLQNVRKLTGKPIPESLVRERGIAI 300 +YEG AA +L+ + +PAVIGPTPIGIRNTDTFL+NV+++TGKPIPESLV+ERGIA+ Sbjct: 241 LNRYEGGLAATWLQTRWDVPAVIGPTPIGIRNTDTFLRNVKEMTGKPIPESLVKERGIAL 300 Query: 301 DALADLVHMFLADKKVAIYGHPDLVIGLAEFCIDLEMQPVLLLLGDDNTTYKSDPRIQAL 360 DA+ DL HMFLADKKVAIYG+PDLV+GLAE+C+DLEM+PVLLLLGD+N Y +DPRI AL Sbjct: 301 DAITDLAHMFLADKKVAIYGNPDLVLGLAEYCLDLEMKPVLLLLGDENAGYANDPRIAAL 360 Query: 361 QQNVEFGMEIVLNADLLELENRIKNKGLELDLILGHSKGRFTAIDNNIPMVRVGFPTYDR 420 + NV++ MEI+ NADL ELE RIKN GLELDLILGHSKGRF AID NIPM+RVGFPTYDR Sbjct: 361 KANVDYPMEIITNADLFELEERIKN-GLELDLILGHSKGRFVAIDYNIPMLRVGFPTYDR 419 Query: 421 AGLYRHPTVGYAGAIRLAEEIANTLFTDMEYKKNKEWILSVW 462 AGLYR+P VGYAGA LAE++ANTLF DME KKN+EWIL+VW Sbjct: 420 AGLYRYPVVGYAGATWLAEQMANTLFNDMEVKKNREWILNVW 461 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.320 0.140 0.411 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 689,281,311 Number of extensions: 29102395 Number of successful extensions: 63770 Number of sequences better than 1.0e-05: 199 Number of HSP's gapped: 63211 Number of HSP's successfully gapped: 205 Length of query: 462 Length of database: 757,237,578 Length adjustment: 129 Effective length of query: 333 Effective length of database: 406,989,807 Effective search space: 135527605731 Effective search space used: 135527605731 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 128 (53.9 bits)