BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0584.0010.p (518 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_00015419.1| COG2710: Nitrogenase molybdenum-iron protein,... 927 0.0 ref|NP_946787.1| putative nitrogenase molybdenum-iron protein al... 905 0.0 ref|ZP_00092170.1| COG2710: Nitrogenase molybdenum-iron protein,... 894 0.0 sp|P16266|ANFD_AZOVI NITROGENASE IRON-IRON PROTEIN ALPHA CHAIN (... 891 0.0 ref|NP_616149.1| nitrogenase, subunit D [Methanosarcina acetivor... 882 0.0 >ref|ZP_00015419.1| COG2710: Nitrogenase molybdenum-iron protein, alpha and beta chains [Rhodospirillum rubrum] Length = 519 Score = 927 bits (2395), Expect = 0.0, Method: Compositional matrix adjust. Identities = 435/513 (84%), Positives = 468/513 (91%), Gaps = 1/513 (0%) Query: 1 MPHHTFKCSACIPERTQHAVIKGPGEDLTSALPEGYLNTIPGTISERGCAYCGAKHVIGT 60 MPHHTFKCS CIPERT+HAVIKG EDLTS LP GYLNTIPG+ISERGCAYCGAKHVIG Sbjct: 1 MPHHTFKCSECIPERTKHAVIKGADEDLTSCLPLGYLNTIPGSISERGCAYCGAKHVIGQ 60 Query: 61 PMKDVIHLSHGPVGCTYDTWQTKRYISDNGNFQLKNTFASDVKEKHIVFGAEGLLKKNII 120 PMKDVIH+SHGP+GCTYDTWQTKRYISDN NFQLK T+A+DV+EKHIVFGAE LLK+NI+ Sbjct: 61 PMKDVIHISHGPIGCTYDTWQTKRYISDNNNFQLKYTYATDVREKHIVFGAEKLLKQNIL 120 Query: 121 EAFTAFPEIKRMTIYSTCATALIGDDIGAVAQEVMDEMPDVDIFVCNSPGFAGPSQSGGH 180 EAF AFP+IKRMTIY TCATALIGDDI A+A EVMDE+PDVDIFVCNSPGFAGPSQSGGH Sbjct: 121 EAFKAFPDIKRMTIYQTCATALIGDDIDAIASEVMDELPDVDIFVCNSPGFAGPSQSGGH 180 Query: 181 HKINIAWINQKVGTVEPEITSDYVINYVGEYNIQGDQEVMADYFKSMGIQVLSTFTGNGS 240 HKINIAW+NQKVGTVEPEITSDYVINYVGEYNIQGDQEVM DYF MGIQ+LSTFTGNGS Sbjct: 181 HKINIAWVNQKVGTVEPEITSDYVINYVGEYNIQGDQEVMQDYFNRMGIQILSTFTGNGS 240 Query: 241 YDDLRAMHRAHLNVLECARSAEYICNELRVKYGIPRLDIDGFGFEPLSASLRKIGLFFGI 300 YD LRAMHRAHLNVLECARSAEYICNELRV+YGIPRLDIDGFGFEPLS SLRKIGLFFGI Sbjct: 241 YDGLRAMHRAHLNVLECARSAEYICNELRVRYGIPRLDIDGFGFEPLSDSLRKIGLFFGI 300 Query: 301 EDRAQAIIDEQTARWKPELDWYKERLQGKRVCLWPGGSKLWHWANVIHEEMGVEVVSLYT 360 EDRAQAIIDE+TA+WKP+LDWYKERL+GK+VCLWPGGSKLWHWA+VI EEMG+ VVSLYT Sbjct: 301 EDRAQAIIDEETAKWKPQLDWYKERLRGKKVCLWPGGSKLWHWAHVIQEEMGLNVVSLYT 360 Query: 361 KFGHQGDMEKGIARCEQGALAIDDPNELEGLEAMEMLKPDIIFTGKRPGEVAKKIRVPYL 420 KFGHQGDMEKGIARC +GALAIDDPNELEGLEA+EMLKPDII TGKRPGEVAKK+RVPYL Sbjct: 361 KFGHQGDMEKGIARCGEGALAIDDPNELEGLEALEMLKPDIILTGKRPGEVAKKVRVPYL 420 Query: 421 NAHAYHNGPYKGYEGWVRFARDIYNAVYSPIHQLSALNISKDEIPTDKGFATQRMVSDVN 480 NAHAYHNGPYKGYEGWVRFARDIYNA+YSPI QLSAL+ISKD IPTD+GF T +M+SD Sbjct: 421 NAHAYHNGPYKGYEGWVRFARDIYNAIYSPIFQLSALDISKDPIPTDQGFLTPQMISDPA 480 Query: 481 L-XXXXXXXXLRQYTGGYDSVSKLRNKTYPHLE 512 L L Y G YD++S LR KTYP + Sbjct: 481 LPAEVRSSTVLTPYRGAYDTISALREKTYPRFD 513 >ref|NP_946787.1| putative nitrogenase molybdenum-iron protein alpha chain (nitrogenase component I) (dinitrogenase) [Rhodopseudomonas palustris CGA009] emb|CAE26880.1| putative nitrogenase molybdenum-iron protein alpha chain (nitrogenase component I) (dinitrogenase) [Rhodopseudomonas palustris CGA009] Length = 522 Score = 905 bits (2339), Expect = 0.0, Method: Compositional matrix adjust. Identities = 424/512 (82%), Positives = 460/512 (89%), Gaps = 1/512 (0%) Query: 1 MPHHTFKCSACIPERTQHAVIKGPGEDLTSALPEGYLNTIPGTISERGCAYCGAKHVIGT 60 MP+H F CS C+PER +HAV KG G++L +ALP GYLNTIPG+ISERGCAYCGAKHVIGT Sbjct: 1 MPYHEFDCSKCLPERQKHAVTKGAGDNLATALPLGYLNTIPGSISERGCAYCGAKHVIGT 60 Query: 61 PMKDVIHLSHGPVGCTYDTWQTKRYISDNGNFQLKNTFASDVKEKHIVFGAEGLLKKNII 120 PMKDVIH+SHGPVGCTYDTWQTKRYISDN NFQLK TFA+DV+EKHIVFGAEGLLK+NII Sbjct: 61 PMKDVIHMSHGPVGCTYDTWQTKRYISDNNNFQLKYTFATDVREKHIVFGAEGLLKQNII 120 Query: 121 EAFTAFPEIKRMTIYSTCATALIGDDIGAVAQEVMDEMPDVDIFVCNSPGFAGPSQSGGH 180 EAF A P+IKRMTIY TCATALIGDDI AVA EVM+EMPDVDIF CNSPGF GPSQSGGH Sbjct: 121 EAFKACPDIKRMTIYQTCATALIGDDINAVAAEVMEEMPDVDIFTCNSPGFGGPSQSGGH 180 Query: 181 HKINIAWINQKVGTVEPEITSDYVINYVGEYNIQGDQEVMADYFKSMGIQVLSTFTGNGS 240 HKINIAWIN KVGTVEPEITSDYVINYVGEYNIQGDQEVM DYF MGIQVLSTFTGNG+ Sbjct: 181 HKINIAWINDKVGTVEPEITSDYVINYVGEYNIQGDQEVMLDYFTRMGIQVLSTFTGNGT 240 Query: 241 YDDLRAMHRAHLNVLECARSAEYICNELRVKYGIPRLDIDGFGFEPLSASLRKIGLFFGI 300 YD LRAMHRAHLNVLECARSAEYICNELRV+YGIPRLDIDGFGFEPLS SLRKIG+FFGI Sbjct: 241 YDGLRAMHRAHLNVLECARSAEYICNELRVRYGIPRLDIDGFGFEPLSQSLRKIGMFFGI 300 Query: 301 EDRAQAIIDEQTARWKPELDWYKERLQGKRVCLWPGGSKLWHWANVIHEEMGVEVVSLYT 360 EDRA+AII E+TARWKPELDWYKERL+GK+VCLWPGGSKLWHWA+ I EEMGV+VVS+YT Sbjct: 301 EDRAEAIIAEETARWKPELDWYKERLKGKKVCLWPGGSKLWHWAHAIEEEMGVKVVSVYT 360 Query: 361 KFGHQGDMEKGIARCEQGALAIDDPNELEGLEAMEMLKPDIIFTGKRPGEVAKKIRVPYL 420 KFGHQGDMEKGIARC + ALAIDDPNELEGLEA+E L+PDIIFTGKRPGEVAKK+RVPYL Sbjct: 361 KFGHQGDMEKGIARCGEDALAIDDPNELEGLEALEKLQPDIIFTGKRPGEVAKKVRVPYL 420 Query: 421 NAHAYHNGPYKGYEGWVRFARDIYNAVYSPIHQLSALNISKDEIPTDKGFATQRMVSDVN 480 NAHAYHNGPYKG+EGWVRFARDIYN +YSP+HQLS L+ISKDEIP D+GF TQRM+SD Sbjct: 421 NAHAYHNGPYKGFEGWVRFARDIYNGIYSPMHQLSGLDISKDEIPADRGFVTQRMLSDAK 480 Query: 481 L-XXXXXXXXLRQYTGGYDSVSKLRNKTYPHL 511 L LR+YTG D +S LR K P+ Sbjct: 481 LPEEIAKSETLRRYTGKDDIISDLRKKNAPYF 512 >ref|ZP_00092170.1| COG2710: Nitrogenase molybdenum-iron protein, alpha and beta chains [Azotobacter vinelandii] Length = 518 Score = 894 bits (2309), Expect = 0.0, Method: Compositional matrix adjust. Identities = 430/518 (83%), Positives = 469/518 (90%), Gaps = 2/518 (0%) Query: 1 MPHHTFKCSACIPERTQHAVIKGPGEDLTSALPEGYLNTIPGTISERGCAYCGAKHVIGT 60 MPHH F+CS IPER +HAVIKG GE L ALP+GYLNTIPG+ISERGCAYCGAKHVIGT Sbjct: 1 MPHHEFECSKVIPERKKHAVIKGKGETLADALPQGYLNTIPGSISERGCAYCGAKHVIGT 60 Query: 61 PMKDVIHLSHGPVGCTYDTWQTKRYISDNGNFQLKNTFASDVKEKHIVFGAEGLLKKNII 120 PMKDVIH+SHGPVGCTYDTWQTKRYISDN NFQLK T+A+DVKEKHIVFGAE LLK+NII Sbjct: 61 PMKDVIHISHGPVGCTYDTWQTKRYISDNDNFQLKYTYATDVKEKHIVFGAEKLLKQNII 120 Query: 121 EAFTAFPEIKRMTIYSTCATALIGDDIGAVAQEVMDEMPDVDIFVCNSPGFAGPSQSGGH 180 EAF AFP+IKRMTIY TCATALIGDDI A+A+EVM+EMP+VDIFVCNSPGFAGPSQSGGH Sbjct: 121 EAFKAFPQIKRMTIYQTCATALIGDDINAIAEEVMEEMPEVDIFVCNSPGFAGPSQSGGH 180 Query: 181 HKINIAWINQKVGTVEPEITSDYVINYVGEYNIQGDQEVMADYFKSMGIQVLSTFTGNGS 240 HKINIAWINQKVGTVEPEIT D+VINYVGEYNIQGDQEVM DYFK MGIQVLSTFTGNGS Sbjct: 181 HKINIAWINQKVGTVEPEITGDHVINYVGEYNIQGDQEVMVDYFKRMGIQVLSTFTGNGS 240 Query: 241 YDDLRAMHRAHLNVLECARSAEYICNELRVKYGIPRLDIDGFGFEPLSASLRKIGLFFGI 300 YD LRAMHRAHLNVLECARSAEYICNELRV+YGIPRLDIDGFGF+PL+ SLRKIG+FFGI Sbjct: 241 YDGLRAMHRAHLNVLECARSAEYICNELRVRYGIPRLDIDGFGFKPLADSLRKIGMFFGI 300 Query: 301 EDRAQAIIDEQTARWKPELDWYKERLQGKRVCLWPGGSKLWHWANVIHEEMGVEVVSLYT 360 EDRA+AIIDE+ ARWKPELDWYKERL GK+VCLWPGGSKLWHWA+VI EEMG++VVS+YT Sbjct: 301 EDRAKAIIDEEVARWKPELDWYKERLMGKKVCLWPGGSKLWHWAHVIEEEMGLKVVSVYT 360 Query: 361 KFGHQGDMEKGIARCEQGALAIDDPNELEGLEAMEMLKPDIIFTGKRPGEVAKKIRVPYL 420 KFGHQGDMEKGIARC +G LAIDDPNELEGLEA+EMLKPDII TGKRPGEVAKK+RVPYL Sbjct: 361 KFGHQGDMEKGIARCGEGTLAIDDPNELEGLEALEMLKPDIILTGKRPGEVAKKVRVPYL 420 Query: 421 NAHAYHNGPYKGYEGWVRFARDIYNAVYSPIHQLSALNISKDEIPT-DKGFATQRMVSDV 479 NAHAYHNGPYKG+EGWVRFARDIYNA+YSPIHQLS ++I+KD P GF T++M+SD Sbjct: 421 NAHAYHNGPYKGFEGWVRFARDIYNAIYSPIHQLSGIDITKDNAPEWGNGFRTRQMLSDG 480 Query: 480 NLX-XXXXXXXLRQYTGGYDSVSKLRNKTYPHLENQLA 516 NL LRQYTGGYDSVSKLR + YP E ++ Sbjct: 481 NLSDAVRNSETLRQYTGGYDSVSKLREREYPAFERKVG 518 >sp|P16266|ANFD_AZOVI NITROGENASE IRON-IRON PROTEIN ALPHA CHAIN (NITROGENASE COMPONENT I) (DINITROGENASE 3 ALPHA SUBUNIT) pir||B32057 nitrogenase (EC 1.18.6.1) 3 alpha chain - Azotobacter vinelandii gb|AAA82509.1| nitrogenase 3 subunit D Length = 518 Score = 891 bits (2303), Expect = 0.0, Method: Compositional matrix adjust. Identities = 429/518 (82%), Positives = 468/518 (90%), Gaps = 2/518 (0%) Query: 1 MPHHTFKCSACIPERTQHAVIKGPGEDLTSALPEGYLNTIPGTISERGCAYCGAKHVIGT 60 MPHH F+CS IPER +HAVIKG GE L ALP+GYLNTIPG+ISERGCAYCGAKHVIGT Sbjct: 1 MPHHEFECSKVIPERKKHAVIKGKGETLADALPQGYLNTIPGSISERGCAYCGAKHVIGT 60 Query: 61 PMKDVIHLSHGPVGCTYDTWQTKRYISDNGNFQLKNTFASDVKEKHIVFGAEGLLKKNII 120 PMKDVIH+SHGPVGCTYDTWQTKRYISDN NFQLK T+A+DVKEKHIVFGAE LLK+NII Sbjct: 61 PMKDVIHISHGPVGCTYDTWQTKRYISDNDNFQLKYTYATDVKEKHIVFGAEKLLKQNII 120 Query: 121 EAFTAFPEIKRMTIYSTCATALIGDDIGAVAQEVMDEMPDVDIFVCNSPGFAGPSQSGGH 180 EAF AFP+IKRMTIY TCATALIGDDI A+A+EVM+EMP+VDIFVCNSPGFAGPSQSGGH Sbjct: 121 EAFKAFPQIKRMTIYQTCATALIGDDINAIAEEVMEEMPEVDIFVCNSPGFAGPSQSGGH 180 Query: 181 HKINIAWINQKVGTVEPEITSDYVINYVGEYNIQGDQEVMADYFKSMGIQVLSTFTGNGS 240 HKINIAWINQKVGTVEPEIT D+VINYVGEYNIQGDQEVM DYFK MGIQVLSTFTGNGS Sbjct: 181 HKINIAWINQKVGTVEPEITGDHVINYVGEYNIQGDQEVMVDYFKRMGIQVLSTFTGNGS 240 Query: 241 YDDLRAMHRAHLNVLECARSAEYICNELRVKYGIPRLDIDGFGFEPLSASLRKIGLFFGI 300 YD LRAMHRAHLNVLECARSAEYICNELRV+YGIPRLDIDGFGF+PL+ SLRKIG+FFGI Sbjct: 241 YDGLRAMHRAHLNVLECARSAEYICNELRVRYGIPRLDIDGFGFKPLADSLRKIGMFFGI 300 Query: 301 EDRAQAIIDEQTARWKPELDWYKERLQGKRVCLWPGGSKLWHWANVIHEEMGVEVVSLYT 360 EDRA+AIIDE+ ARWKPELDWYKERL GK+VCLWPGGSKLWHWA+VI EEMG++VVS+Y Sbjct: 301 EDRAKAIIDEEVARWKPELDWYKERLMGKKVCLWPGGSKLWHWAHVIEEEMGLKVVSVYI 360 Query: 361 KFGHQGDMEKGIARCEQGALAIDDPNELEGLEAMEMLKPDIIFTGKRPGEVAKKIRVPYL 420 KFGHQGDMEKGIARC +G LAIDDPNELEGLEA+EMLKPDII TGKRPGEVAKK+RVPYL Sbjct: 361 KFGHQGDMEKGIARCGEGTLAIDDPNELEGLEALEMLKPDIILTGKRPGEVAKKVRVPYL 420 Query: 421 NAHAYHNGPYKGYEGWVRFARDIYNAVYSPIHQLSALNISKDEIPT-DKGFATQRMVSDV 479 NAHAYHNGPYKG+EGWVRFARDIYNA+YSPIHQLS ++I+KD P GF T++M+SD Sbjct: 421 NAHAYHNGPYKGFEGWVRFARDIYNAIYSPIHQLSGIDITKDNAPEWGNGFRTRQMLSDG 480 Query: 480 NLX-XXXXXXXLRQYTGGYDSVSKLRNKTYPHLENQLA 516 NL LRQYTGGYDSVSKLR + YP E ++ Sbjct: 481 NLSDAVRNSETLRQYTGGYDSVSKLREREYPAFERKVG 518 >ref|NP_616149.1| nitrogenase, subunit D [Methanosarcina acetivorans str. C2A] gb|AAM04629.1| nitrogenase, subunit D [Methanosarcina acetivorans str. C2A] Length = 520 Score = 882 bits (2279), Expect = 0.0, Method: Compositional matrix adjust. Identities = 406/519 (78%), Positives = 458/519 (88%), Gaps = 1/519 (0%) Query: 1 MPHHTFKCSACIPERTQHAVIKGPGEDLTSALPEGYLNTIPGTISERGCAYCGAKHVIGT 60 MP+HTF+CS CIPER HAVIKGPGEDLTS LP GYLNTIPGTISERGCAYCGAKHVIG Sbjct: 1 MPYHTFECSECIPERAMHAVIKGPGEDLTSCLPLGYLNTIPGTISERGCAYCGAKHVIGA 60 Query: 61 PMKDVIHLSHGPVGCTYDTWQTKRYISDNGNFQLKNTFASDVKEKHIVFGAEGLLKKNII 120 PMKDVIH+SHGPVGCTYDTW TKRYISDN NFQLK TFA+D+KEKH+VFGAE +LK+NII Sbjct: 61 PMKDVIHVSHGPVGCTYDTWHTKRYISDNDNFQLKYTFATDMKEKHVVFGAEKMLKQNII 120 Query: 121 EAFTAFPEIKRMTIYSTCATALIGDDIGAVAQEVMDEMPDVDIFVCNSPGFAGPSQSGGH 180 + F AFP IKRMTIY TCA+ALIGDDI AVA++VM EMPDVDIFVCN+PGF GPSQSGGH Sbjct: 121 DCFKAFPHIKRMTIYQTCASALIGDDIAAVAKKVMAEMPDVDIFVCNAPGFGGPSQSGGH 180 Query: 181 HKINIAWINQKVGTVEPEITSDYVINYVGEYNIQGDQEVMADYFKSMGIQVLSTFTGNGS 240 HKINIAWI+QKVGT EPEI S YVINYVG+YNIQGD E+M DYF+ MGIQVLSTFTGNGS Sbjct: 181 HKINIAWIDQKVGTYEPEIKSKYVINYVGDYNIQGDAEIMVDYFQRMGIQVLSTFTGNGS 240 Query: 241 YDDLRAMHRAHLNVLECARSAEYICNELRVKYGIPRLDIDGFGFEPLSASLRKIGLFFGI 300 YDDLR MH AHLNVLECARSAEYICNELR +YG PRLDIDG+GFEPLSASL K+ +FFG+ Sbjct: 241 YDDLRGMHLAHLNVLECARSAEYICNELRKRYGTPRLDIDGYGFEPLSASLMKVAMFFGL 300 Query: 301 EDRAQAIIDEQTARWKPELDWYKERLQGKRVCLWPGGSKLWHWANVIHEEMGVEVVSLYT 360 E AQAIIDE+TARW+PEL+WY +RL+GK++CLWPGGSKLWHWA+VI EEMGV+VVS+Y+ Sbjct: 301 EKEAQAIIDEETARWRPELNWYAKRLKGKKICLWPGGSKLWHWAHVIEEEMGVKVVSVYS 360 Query: 361 KFGHQGDMEKGIARCEQGALAIDDPNELEGLEAMEMLKPDIIFTGKRPGEVAKKIRVPYL 420 KFGHQGD EKG+ARC +GALAIDDPNELEGLEAMEML+PD + TG RPGEV+KK+R+PYL Sbjct: 361 KFGHQGDFEKGVARCSEGALAIDDPNELEGLEAMEMLQPDCVLTGVRPGEVSKKMRIPYL 420 Query: 421 NAHAYHNGPYKGYEGWVRFARDIYNAVYSPIHQLSALNISKDEIPTDKGFATQRMVSDVN 480 N H YHNGPYKGYEGWVR ARD+YNA+YSPIHQLS ++ISKDEIPTDKGF T++M+SDVN Sbjct: 421 NIHGYHNGPYKGYEGWVRLARDLYNAIYSPIHQLSGMDISKDEIPTDKGFLTRQMISDVN 480 Query: 481 LXXXXXX-XXLRQYTGGYDSVSKLRNKTYPHLENQLAGV 518 + R YTG +D V++LR KTYP LE+Q G Sbjct: 481 IVDDRTTPSEERPYTGDWDIVTRLRGKTYPKLESQQLGT 519 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.319 0.137 0.426 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 820,453,937 Number of extensions: 35759683 Number of successful extensions: 71672 Number of sequences better than 1.0e-05: 327 Number of HSP's gapped: 70669 Number of HSP's successfully gapped: 336 Length of query: 518 Length of database: 757,237,578 Length adjustment: 130 Effective length of query: 388 Effective length of database: 404,274,708 Effective search space: 156858586704 Effective search space used: 156858586704 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 128 (53.9 bits)