BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0584.0005.p (439 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_00160873.1| hypothetical protein Avar237701 [Anabaena var... 290 5e-77 gb|AAA93024.1| NifEN2 289 7e-77 ref|ZP_00112341.1| COG2710: Nitrogenase molybdenum-iron protein,... 279 1e-73 ref|ZP_00054382.1| COG2710: Nitrogenase molybdenum-iron protein,... 271 3e-71 sp|O07356|NIFN_SYNP8 Nitrogenase iron-molybdenum cofactor biosyn... 270 4e-71 >ref|ZP_00160873.1| hypothetical protein Avar237701 [Anabaena variabilis ATCC 29413] Length = 905 Score = 290 bits (742), Expect = 5e-77, Method: Compositional matrix adjust. Identities = 161/425 (37%), Positives = 246/425 (57%), Gaps = 8/425 (1%) Query: 6 RTAAKPLSVNPFQTSQIIGAVLALQGIYRAVPILHGAQGCAESVKTVLSRHFREPISIQN 65 + + K ++VNP + SQ +GA LA G+ +P+ HG+QGC K +L RHFRE I + Sbjct: 476 QNSHKSVAVNPLKQSQPLGAALAFLGLKGVMPLFHGSQGCTAFAKVMLVRHFREAIPLST 535 Query: 66 IAIHEQNLIFGGEKGIRDMIRLVVEKYNPDVLALIGTSITEVVGEDLLGTAEGYYRQHKM 125 A+ E I GGE I I +VEK P+++ L+ T +TE G+D+ G ++H Sbjct: 536 TAMTEVTTILGGEDNIEQAILTLVEKSKPEIIGLLTTGLTETRGDDMEGILRSIRKRHPE 595 Query: 126 MFKDKLMCGLYLPDYEGSMESGYAKSVYAVIQKVIQNSGXXXXXXXXXXXXLLPGAHLTP 185 ++ D + PD++G+++ G+A +V ++++++ Q +L + TP Sbjct: 596 LY-DLPIIFASTPDFQGALQDGFATAVESIVKEIPQPG-----ETRLDQINILVSSAFTP 649 Query: 186 GDVMELKEMIASFDIEVIVLPDLSSSLTGHLMTGHTPLSRGGVPLDYLREMLTSTYTLAL 245 GD+ E+KE++++F +E IV+PD S+SL GHL ++ ++ GG L LR+M +S +TLAL Sbjct: 650 GDIQEIKEIVSAFGLETIVVPDHSTSLDGHLDDSYSAVTGGGTTLAELRQMGSSVFTLAL 709 Query: 246 GGSMEACAKLLKEELQIPYQVFQGVTGLRETDEFFLFLRQFSQSDGQNKYRWQRQFLLDC 305 G SM A+ L+ + IPY+VF +TGL D F L S + KYR QR+ L D Sbjct: 710 GESMRRAAESLQTQFGIPYEVFPQLTGLDAVDNFLQGLVDISGNAVPEKYRHQRRQLQDA 769 Query: 306 MLDTRSVFRGKRIIAALEPDHLIALYQCLAELGIKSVGSVIPAGSPAVAAMP-EAVQTGD 364 MLDT F KR+ ALEPD L ++ LA +G + +V SP + +P E V GD Sbjct: 770 MLDTHFYFGRKRVSLALEPDLLWSIASFLASMGAEIHAAVTTTKSPLLEKLPVEKVTIGD 829 Query: 365 LEDLERMAAGGADLWVSNAYGERHALKSGIPFLPLGFPVFNRFGAPANISVGYRGTADLL 424 LED ER+A G+DL ++N++G+ + + F LGFP+F+R G +VGYRGT LL Sbjct: 830 LEDFERLAV-GSDLVIANSHGKAISRRLQTSFYRLGFPIFDRLGNGQRCTVGYRGTTQLL 888 Query: 425 NNIAN 429 +I N Sbjct: 889 FDIGN 893 >gb|AAA93024.1| NifEN2 Length = 905 Score = 289 bits (740), Expect = 7e-77, Method: Compositional matrix adjust. Identities = 161/425 (37%), Positives = 246/425 (57%), Gaps = 8/425 (1%) Query: 6 RTAAKPLSVNPFQTSQIIGAVLALQGIYRAVPILHGAQGCAESVKTVLSRHFREPISIQN 65 + + K ++VNP + SQ +GA LA G+ +P+ HG+QGC K +L RHFRE I + Sbjct: 476 QNSHKSVAVNPLKQSQPLGAALAFLGLKGVMPLFHGSQGCTAFAKVMLVRHFREAIPLST 535 Query: 66 IAIHEQNLIFGGEKGIRDMIRLVVEKYNPDVLALIGTSITEVVGEDLLGTAEGYYRQHKM 125 A+ E I GGE I I +VEK P+++ L+ T +TE G+D+ G ++H Sbjct: 536 TAMTEVTTILGGEDNIEQAILTLVEKSKPEIIGLLTTGLTETRGDDMEGILRSIRKRHPE 595 Query: 126 MFKDKLMCGLYLPDYEGSMESGYAKSVYAVIQKVIQNSGXXXXXXXXXXXXLLPGAHLTP 185 ++ D + PD++G+++ G+A +V ++++++ Q +L + TP Sbjct: 596 LY-DLPIIFASTPDFQGALQDGFATAVESIVKEIPQPG-----ETRLDQINILVSSAFTP 649 Query: 186 GDVMELKEMIASFDIEVIVLPDLSSSLTGHLMTGHTPLSRGGVPLDYLREMLTSTYTLAL 245 GD+ E+KE++++F +E IV+PD S+SL GHL ++ ++ GG L LR+M +S +TLAL Sbjct: 650 GDIQEIKEIVSAFGLETIVVPDHSTSLDGHLDDSYSAVTGGGTTLAELRQMGSSVFTLAL 709 Query: 246 GGSMEACAKLLKEELQIPYQVFQGVTGLRETDEFFLFLRQFSQSDGQNKYRWQRQFLLDC 305 G SM A+ L+ + IPY+VF +TGL D F L S + KYR QR+ L D Sbjct: 710 GESMRRAAESLQTQFGIPYEVFPQLTGLDAVDNFLQGLVDISGNAVPEKYRHQRRQLQDA 769 Query: 306 MLDTRSVFRGKRIIAALEPDHLIALYQCLAELGIKSVGSVIPAGSPAVAAMP-EAVQTGD 364 MLDT F KR+ ALEPD L ++ LA +G + +V SP + +P E V GD Sbjct: 770 MLDTHFYFGRKRVSLALEPDLLWSIASFLASMGAEIHAAVTTTKSPLLEKLPVEKVTIGD 829 Query: 365 LEDLERMAAGGADLWVSNAYGERHALKSGIPFLPLGFPVFNRFGAPANISVGYRGTADLL 424 LED ER+A G+DL ++N++G+ + + F LGFP+F+R G +VGYRGT LL Sbjct: 830 LEDFERLAV-GSDLDIANSHGKAISRRLQTSFYRLGFPIFDRLGNGQRCTVGYRGTTQLL 888 Query: 425 NNIAN 429 +I N Sbjct: 889 FDIGN 893 >ref|ZP_00112341.1| COG2710: Nitrogenase molybdenum-iron protein, alpha and beta chains [Nostoc punctiforme] Length = 445 Score = 279 bits (713), Expect = 1e-73, Method: Compositional matrix adjust. Identities = 160/421 (38%), Positives = 235/421 (55%), Gaps = 8/421 (1%) Query: 10 KPLSVNPFQTSQIIGAVLALQGIYRAVPILHGAQGCAESVKTVLSRHFREPISIQNIAIH 69 K L+VNP + SQ +GA LA G+ +P+ HG+QGC K VL RHFRE I + A+ Sbjct: 9 KALTVNPLKQSQALGATLAFLGLKGTMPLFHGSQGCTAFAKVVLVRHFREAIPLATTAMT 68 Query: 70 EQNLIFGGEKGIRDMIRLVVEKYNPDVLALIGTSITEVVGEDLLGTAEGYYRQHKMMFKD 129 E I GGE+ + I +VEK NP+++ L T +TE G+D+ G + +H + D Sbjct: 69 EVTTILGGEENVEQAILTLVEKANPEIIGLCSTGLTETRGDDIEGFLKEIRDRHPEL-ND 127 Query: 130 KLMCGLYLPDYEGSMESGYAKSVYAVIQKVIQNSGXXXXXXXXXXXXLLPGAHLTPGDVM 189 + PD++G+++ G+A +V ++++++ + G +L G+ TPGDV Sbjct: 128 LAIVFAPTPDFKGALQDGFAVAVESIVKEIPRAGGLRTEQVT-----ILAGSAFTPGDVQ 182 Query: 190 ELKEMIASFDIEVIVLPDLSSSLTGHLMTGHTPLSRGGVPLDYLREMLTSTYTLALGGSM 249 E+KE++ SF + I +PDL +SL GHL ++ ++ G L LRE+ +S +TLALG SM Sbjct: 183 EIKEIVTSFGLVPIFVPDLGASLDGHLEDNYSAVTVSGTTLKQLREVGSSAFTLALGESM 242 Query: 250 EACAKLLKEELQIPYQVFQGVTGLRETDEFFLFLRQFSQSDGQNKYRWQRQFLLDCMLDT 309 AK+L+E PY+VF +TGL DEF L S + KYR QR+ L D MLDT Sbjct: 243 RGAAKILEERFGTPYEVFGELTGLEPVDEFIQALAILSGNSVPEKYRRQRRQLQDAMLDT 302 Query: 310 RSVFRGKRIIAALEPDHLIALYQCLAELGIKSVGSVIPAGSPAVAAMP-EAVQTGDLEDL 368 F KR+ ALEPD L + L +G + V S + +P +++ GDLED Sbjct: 303 HFYFGAKRVSLALEPDLLWSTVHFLQSMGSQIHAVVTTTRSHLLEKLPVKSITIGDLEDF 362 Query: 369 ERMAAGGADLWVSNAYGERHALKSGIPFLPLGFPVFNRFGAPANISVGYRGTADLLNNIA 428 E + AGG+DL + N+ A + IP LG P+++R G VGYRGT +LL I Sbjct: 363 ESL-AGGSDLLIGNSNVGAIAKRLSIPLYRLGLPIYDRLGNGQFTKVGYRGTMELLFGIG 421 Query: 429 N 429 N Sbjct: 422 N 422 >ref|ZP_00054382.1| COG2710: Nitrogenase molybdenum-iron protein, alpha and beta chains [Magnetospirillum magnetotacticum] Length = 466 Score = 271 bits (692), Expect = 3e-71, Method: Compositional matrix adjust. Identities = 154/425 (36%), Positives = 231/425 (54%), Gaps = 7/425 (1%) Query: 10 KPLSVNPFQTSQIIGAVLALQGIYRAVPILHGAQGCAESVKTVLSRHFREPISIQNIAIH 69 K LS +P + S +GA LA G+ +P+ HG+QGC ++ RHFRE I +Q A++ Sbjct: 23 KALSTSPLKVSAPLGAALAFMGMKGCLPMFHGSQGCTAFALVMMVRHFREAIPLQTTAMN 82 Query: 70 EQNLIFGGEKGIRDMIRLVVEKYNPDVLALIGTSITEVVGEDLLGTAEGYYRQHKMMFKD 129 E + I GG + + + + ++ P+++ LI T++TE GED+ G + R D Sbjct: 83 EISTILGGMDQVEEGLLNIAKRAKPEIIGLISTALTETRGEDMAGDVK-VIRARNKELAD 141 Query: 130 KLMCGLYLPDYEGSMESGYAKSVYAVIQKVIQNSGXXXXXXXXXXXXLLPGAHLTPGDVM 189 + + PD+ GS+E+G+ K+V A+I+ + + SG LLPGAHLTPGDV Sbjct: 142 IAVVDVSAPDFSGSLETGWGKAVTAIIKSLAKPSGNHAKLDRVN---LLPGAHLTPGDVE 198 Query: 190 ELKEMIASFDIEVIVLPDLSSSLTGHLMTGHTPLSRGGVPLDYLREMLTSTYTLALGGSM 249 LK+++A F +E +VLPDLS SL GH+ +TP + GG + +REM + T+A+G M Sbjct: 199 ALKDIVADFGMEAMVLPDLSDSLDGHVPDSYTPTTLGGTAVASVREMGRARLTIAIGEHM 258 Query: 250 EACAKLLKEELQIPYQVFQGVTGLRETDEFFLFLRQFSQSDGQNKYRWQRQFLLDCMLDT 309 AK + +P V VTGL D + L + + R R L+D MLD Sbjct: 259 RIAAKTAENITGVPSVVLDRVTGLEAVDRLMVSLSTVAGVPVPARQRRARSQLVDAMLDG 318 Query: 310 RSVFRGKRIIAALEPDHLIALYQCLAELGIKSVGSVIPAGSPAVAAM-PEAVQTGDLEDL 368 F G+ + A EP L ++ + ++++G +V P +A + E V GD EDL Sbjct: 319 HFYFGGRSVAIAAEPALLWSMGKLVSDMGATIAAAVTTQPVPMLAELKAERVIVGDFEDL 378 Query: 369 ER--MAAGGADLWVSNAYGERHALKSGIPFLPLGFPVFNRFGAPANISVGYRGTADLLNN 426 E + AGGADL ++N+ G + A GIP GFPVF+R GA S GYRGT DL+ Sbjct: 379 EDQILEAGGADLIIANSNGRQVAAHHGIPLFRAGFPVFDRLGAAHLTSAGYRGTRDLITQ 438 Query: 427 IANTM 431 + N + Sbjct: 439 LGNIL 443 >sp|O07356|NIFN_SYNP8 Nitrogenase iron-molybdenum cofactor biosynthesis protein nifN gb|AAC35195.1| NifN [Cyanothece sp. PCC 8801] Length = 461 Score = 270 bits (691), Expect = 4e-71, Method: Compositional matrix adjust. Identities = 158/444 (35%), Positives = 239/444 (53%), Gaps = 17/444 (3%) Query: 10 KPLSVNPFQTSQIIGAVLALQGIYRAVPILHGAQGCAESVKTVLSRHFREPISIQNIAIH 69 KPLSVNP + SQ +GA LA G+ +P+ HGAQGC K VL RHFRE I + A+ Sbjct: 9 KPLSVNPLKMSQPLGASLAFLGLKGMMPLFHGAQGCTAFAKVVLVRHFRESIPLSTTAMT 68 Query: 70 EQNLIFGGEKGIRDMIRLVVEKYNPDVLALIGTSITEVVGEDLLGTAEGYYRQHKMMFKD 129 E + I GG+ + I +V+K P+++ L+ T +TE G+D+ G + ++H + K+ Sbjct: 69 EVSTILGGQDHVEQAILTIVDKNKPEIIGLLTTGLTETRGDDMEGILKDIRQKHPQL-KN 127 Query: 130 KLMCGLYLPDYEGSMESGYAKSVYAVI----QKVIQNSGXXXXXXXXXXXXLLPGAHLTP 185 + + PDY+GS++ YA +V ++ I + +L G+ L+P Sbjct: 128 LPIVFVSTPDYKGSLQDCYAATVEQIVATDYNAFIAENARSAVIYPQPQVTVLAGSSLSP 187 Query: 186 GDVMELKEMIASFDIEVIVLPDLSSSLTGHLMTGHTPLSRGGVPLDYLREMLTSTYTLAL 245 GD+ E+K +I +F + +V+PDLS SL GHL G+ ++ GG L LR + S YTLA+ Sbjct: 188 GDIQEIKSIIEAFGLMPLVIPDLSRSLDGHLEDGYQSITGGGTTLPQLRSLPHSCYTLAI 247 Query: 246 GGSMEACAKLLKEELQIPYQVFQGVTGLRETDEFFLFLRQFSQSDGQNKYRW-------- 297 G SM A++LK+ Y+VF + GL D F L Q S + + + Sbjct: 248 GESMRGAAEILKDRFGTNYEVFPRLAGLEAVDTFLWRLSQIVTSRCDHHFPFVPNIPALF 307 Query: 298 --QRQFLLDCMLDTRSVFRGKRIIAALEPDHLIALYQCLAELGIKSVGSVIPAGSPAVAA 355 QR+ L D +LDT F GK++ ALEPD L L E+G K +V SP + Sbjct: 308 ERQRRQLQDAILDTHFYFGGKKVALALEPDLLHQTAWLLTEMGAKIQAAVTTTKSPLLED 367 Query: 356 MP-EAVQTGDLEDLERMAAGGADLWVSNAYGERHALKSGIPFLPLGFPVFNRFGAPANIS 414 +P + V GDLEDLE ++A G DL ++N++G A + P +G+PVF++ G Sbjct: 368 LPVDTVTIGDLEDLEDLSA-GVDLIITNSHGTAMAQRLNAPLYRMGYPVFDQLGNGQRCL 426 Query: 415 VGYRGTADLLNNIANTMNSKGRNR 438 VGYRGT L ++ N + ++ N Sbjct: 427 VGYRGTIQFLFDVGNILLAEEANH 450 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.320 0.138 0.402 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 628,856,985 Number of extensions: 25491239 Number of successful extensions: 55566 Number of sequences better than 1.0e-05: 147 Number of HSP's gapped: 55165 Number of HSP's successfully gapped: 150 Length of query: 439 Length of database: 757,237,578 Length adjustment: 129 Effective length of query: 310 Effective length of database: 406,989,807 Effective search space: 126166840170 Effective search space used: 126166840170 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 127 (53.5 bits)