BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0584.0004.p (453 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_00073545.1| COG2710: Nitrogenase molybdenum-iron protein,... 368 e-100 ref|ZP_00150895.1| hypothetical protein Daro255601 [Dechloromona... 367 e-100 sp|O07355|NIFE_SYNP8 Nitrogenase iron-molybdenum cofactor biosyn... 363 4e-99 emb|CAC42171.1| NifE protein [Pseudomonas stutzeri] 362 1e-98 ref|ZP_00090766.1| COG2710: Nitrogenase molybdenum-iron protein,... 357 4e-97 >ref|ZP_00073545.1| COG2710: Nitrogenase molybdenum-iron protein, alpha and beta chains [Trichodesmium erythraeum IMS101] gb|AAF82640.1| NifEN [Trichodesmium sp. IMS101] Length = 936 Score = 368 bits (945), Expect = e-100, Method: Compositional matrix adjust. Identities = 187/454 (41%), Positives = 281/454 (61%), Gaps = 11/454 (2%) Query: 2 PKGVPNLFIEPDCEHNRQPG------KGCARPNPGEVTKG-CVFQGSQAVLLPISDAAHL 54 P V L EP CEHN Q KGC + +G C F G+ L+PI+DAAHL Sbjct: 6 PGKVAELLNEPGCEHNHQKNNGDKKQKGCQQQAAPGAAQGGCAFDGASIALVPITDAAHL 65 Query: 55 VHGTSGCLQNSWGMLEAAMPEGPLPYLRFSMGLTETDIILGGQKKLLEAIQYIIEYYHPP 114 VHG GC NSWG + L + F+ + E DII+GG+KKLL AI + + Y PP Sbjct: 66 VHGPLGCSGNSWGARGSLSSSSHLYKMGFTTDMGENDIIMGGEKKLLRAIAELKKRYQPP 125 Query: 115 CIFVYATCITILTAEDLNAVCSKAESLWKLPVIPLHNSGFTGSGNMGSRQAGEALFEKVI 174 IFVYATC+T L +DL VC A ++PVIP+++ GF GS N+G+R EAL + V+ Sbjct: 126 AIFVYATCVTALIGDDLETVCKVATKKLEIPVIPVNSPGFIGSKNLGNRVGAEALLDHVV 185 Query: 175 GTCEPDPRKLTPFDINLIGDYHFSKAGHEIEAMLSKIGIRVISRIAGECTFDELCSAHQA 234 GT EP+ TP+DINLIG+Y+ + + + K+GIRV+S+I G+ T+ E+C AH+A Sbjct: 186 GTAEPE--FTTPYDINLIGEYNIAGEMWAMLPLFEKVGIRVLSKITGDATYKEVCYAHRA 243 Query: 235 KLNLLVCSRSMVTLARKMRDSFGIPYFEGSFCGSREIRFALRQLSFHFQNEGLDKQLNRY 294 KLN+++CS++M+ +ARKM + +GIPY E SF G +I LR ++ + GL ++ + Sbjct: 244 KLNVMICSKAMINMARKMEEEYGIPYIEESFYGVADINNCLRNIAAKIGDAGLQERTEKL 303 Query: 295 MRREEERLHKDILRRYKP-LKGKKVVLFTDGHESWIYISLLYELGIKIAAIGTNQNAQED 353 + EE + ++IL Y+ LKGKKVVL+T G +SW IS +L + + A T ++ +ED Sbjct: 304 I-AEETTILEEILEPYRQRLKGKKVVLYTGGVKSWSIISAAKDLEMDVVATSTKKSTEED 362 Query: 354 LSRIKERIHDDTLLIIQSDETRILQLYRERKGDLMIVSGRNSFVPLKEKIPFINIGEEQH 413 ++IK+ + D +L+ + + +L++ E K D++I GRN + LK +IPF++I +E+H Sbjct: 363 KAKIKKLLGKDGILLEKGNAEILLKVIAETKADMLIAGGRNQYTALKARIPFLHINQERH 422 Query: 414 GGYAGYDGVRRFAQDVMDTLAQPVWTMIRRHSPW 447 YAGY G+ A+++ + L PVW +R+ +PW Sbjct: 423 HPYAGYHGMIEMAKELDEALYSPVWEQVRQPAPW 456 >ref|ZP_00150895.1| hypothetical protein Daro255601 [Dechloromonas aromatica RCB] Length = 466 Score = 367 bits (941), Expect = e-100, Method: Compositional matrix adjust. Identities = 173/449 (38%), Positives = 276/449 (61%), Gaps = 6/449 (1%) Query: 5 VPNLFIEPDCEHNRQPGKGCARPNPGEVTKGCVFQGSQAVLLPISDAAHLVHGTSGCLQN 64 + L EP C HN++ GCA+P PG GC F G+Q LLPI+D AH+VHG C + Sbjct: 6 IQALLDEPACSHNKKEKSGCAKPKPGATAGGCSFDGAQIALLPIADVAHIVHGPIACAGS 65 Query: 65 SWGMLEAAMPEGPLPYLRFSMGLTETDIILG-GQKKLLEAIQYIIEYYHPPCIFVYATCI 123 SW L + + L+ETD+++G G+K+L AI+ I+ Y P +F+Y TC+ Sbjct: 66 SWDNRGTRSSGVTLYKIGMTTDLSETDVVMGRGEKRLFHAIKQAIDSYSPSAVFIYNTCV 125 Query: 124 TILTAEDLNAVCSKAESLWKLPVIPLHNSGFTGSGNMGSRQAGEALFEKVIGTCEPDPRK 183 T L +D+ AVC A W PV+P+ +GF G+ N+G+R AGEA+F+ VIGT EP P Sbjct: 126 TALIGDDVGAVCKAATERWGTPVVPVDAAGFYGTKNLGNRLAGEAMFKHVIGTAEPAPAA 185 Query: 184 -----LTPFDINLIGDYHFSKAGHEIEAMLSKIGIRVISRIAGECTFDELCSAHQAKLNL 238 L +D+NLIG+Y+ + + + ++G+R++ ++G+ F E+ + H+A++N+ Sbjct: 186 PRADGLPTYDVNLIGEYNIAGEFWHVAPLFDELGLRILCTLSGDSRFHEVQTMHRARVNM 245 Query: 239 LVCSRSMVTLARKMRDSFGIPYFEGSFCGSREIRFALRQLSFHFQNEGLDKQLNRYMRRE 298 +VC+++++ +ARKM D+FGIP+FEGSF G +++ ALR + + L + + RE Sbjct: 246 VVCAKALLNVARKMEDNFGIPFFEGSFYGVQDVSNALRDFARLIGDPDLTARTEAVIARE 305 Query: 299 EERLHKDILRRYKPLKGKKVVLFTDGHESWIYISLLYELGIKIAAIGTNQNAQEDLSRIK 358 E + H + L+GK+V+L+T G +SW +S L +LG+K+ A GT ++ +ED +RI+ Sbjct: 306 EAKSHAALEPWRDRLRGKRVLLYTGGVKSWSIVSALQDLGMKVVATGTKKSTEEDKARIR 365 Query: 359 ERIHDDTLLIIQSDETRILQLYRERKGDLMIVSGRNSFVPLKEKIPFINIGEEQHGGYAG 418 E + DDT +I +L Y E K D++I GRN + LK +IPF++I +E+ GYAG Sbjct: 366 ELMGDDTKMIDDGSPKALLSTYHEYKADILIAGGRNLYTALKARIPFLDINQEREFGYAG 425 Query: 419 YDGVRRFAQDVMDTLAQPVWTMIRRHSPW 447 YDG+ A+ + ++ PVW +R+ +PW Sbjct: 426 YDGMVELARQLALSMESPVWAAVRKPAPW 454 >sp|O07355|NIFE_SYNP8 Nitrogenase iron-molybdenum cofactor biosynthesis protein nifE gb|AAC35194.1| NifE [Cyanothece sp. PCC 8801] Length = 469 Score = 363 bits (933), Expect = 4e-99, Method: Compositional matrix adjust. Identities = 175/457 (38%), Positives = 290/457 (63%), Gaps = 12/457 (2%) Query: 1 MPKGVPN-LFIEPDCEHN-RQPGKGCA-----RPNPGEVTKGCVFQGSQAVLLPISDAAH 53 + KG N L +P CEHN + G+G R N + GC F G+ L+PI+DAAH Sbjct: 3 LTKGKINELLTQPGCEHNHNKEGQGKTNLVPNRLNLAQHKGGCAFDGASIALVPITDAAH 62 Query: 54 LVHGTSGCLQNSWGMLEAAMPEGPLPY-LRFSMGLTETDIILGGQKKLLEAIQYIIEYYH 112 LVHG+ C NSW ++ GP+ Y + F+ L+E D+I GG+KKL +AI +++ YH Sbjct: 63 LVHGSIACSGNSWNS-RGSLSSGPMTYKMGFTTDLSENDVIFGGEKKLYQAIAQLVKRYH 121 Query: 113 PPCIFVYATCITILTAEDLNAVCSKAESLWKLPVIPLHNSGFTGSGNMGSRQAGEALFEK 172 P +FVY+TC+T L +DL+AVC A ++ P+IP+H GF GS N+G+R GEAL + Sbjct: 122 PAAVFVYSTCVTALIGDDLDAVCKAATKKYETPIIPVHAPGFVGSKNLGNRLGGEALLDH 181 Query: 173 VIGTCEPDPRKLTPFDINLIGDYHFSKAGHEIEAMLSKIGIRVISRIAGECTFDELCSAH 232 V+GT EP+ T FDINLIG+Y+ + + + K+GIRV+++I G+ + E+C AH Sbjct: 182 VVGTREPE--FTTDFDINLIGEYNVAGEMWGVLPLFEKLGIRVLAKITGDARY-EVCYAH 238 Query: 233 QAKLNLLVCSRSMVTLARKMRDSFGIPYFEGSFCGSREIRFALRQLSFHFQNEGLDKQLN 292 +AKLNL++CS++++ +A M++ +GIPY E SF G ++ LR ++ +F + L +++ Sbjct: 239 RAKLNLMICSKALINMATAMQERYGIPYIEESFYGIADMNRCLRNIAEYFGDAALKERVE 298 Query: 293 RYMRREEERLHKDILRRYKPLKGKKVVLFTDGHESWIYISLLYELGIKIAAIGTNQNAQE 352 + + E +L + + LKGK+VVL+T G +SW +S +LG+++ A T ++ +E Sbjct: 299 QLIEEETTKLDLALAPYRERLKGKRVVLYTGGVKSWSVVSAAQDLGMEVVATSTKKSTEE 358 Query: 353 DLSRIKERIHDDTLLIIQSDETRILQLYRERKGDLMIVSGRNSFVPLKEKIPFINIGEEQ 412 D ++I+E + D +++ + T +L++ + K DL++ GRN + LK +IPF++I +E+ Sbjct: 359 DKAKIRELLGKDGIMLEKGSPTELLRVVEQTKADLLVAGGRNQYTALKARIPFLDINQER 418 Query: 413 HGGYAGYDGVRRFAQDVMDTLAQPVWTMIRRHSPWEV 449 H YAGY G+ A+++ + + P+W ++R+ SPW++ Sbjct: 419 HHPYAGYVGMIEMARELDEAVHSPIWRLVRQPSPWDI 455 >emb|CAC42171.1| NifE protein [Pseudomonas stutzeri] Length = 472 Score = 362 bits (929), Expect = 1e-98, Method: Compositional matrix adjust. Identities = 175/455 (38%), Positives = 278/455 (61%), Gaps = 10/455 (2%) Query: 3 KGVPNLFIEPDCEHNRQPGKGCARPNPGEVTKGCVFQGSQAVLLPISDAAHLVHGTSGCL 62 K + L EP C HN++ GCA+P PG GC F G+Q LLPI+D AH+VHG C Sbjct: 4 KDIAELLDEPACTHNKKEKSGCAKPAPGATDGGCAFDGAQIALLPIADVAHIVHGPIACA 63 Query: 63 QNSWGMLEAAMPEGPLPY-LRFSMGLTETDIILG-GQKKLLEAIQYIIEYYHPPCIFVYA 120 +SW GP Y + + L+E D+I+G +K+L AI+ +E Y PP +FVY Sbjct: 64 GSSWDN-RGTRSSGPQLYRIGMTTDLSEQDVIMGRAEKRLFHAIRQAVESYAPPAVFVYN 122 Query: 121 TCITILTAEDLNAVCSKAESLWKLPVIPLHNSGFTGSGNMGSRQAGEALFEKVIGTCEPD 180 TC+ L +DL+AVC A + PV+P+ +GF G+ N+G+R AG+A+ + VIGT EPD Sbjct: 123 TCVPALIGDDLDAVCKAASEHFATPVVPVDGAGFYGTKNLGNRIAGDAMVKHVIGTREPD 182 Query: 181 P-------RKLTPFDINLIGDYHFSKAGHEIEAMLSKIGIRVISRIAGECTFDELCSAHQ 233 P + D+NLIG+Y+ + + +L ++G+RV+ ++G+ F E+ + H+ Sbjct: 183 PLPAGAERAGIRVHDVNLIGEYNIAGEFWHVLPLLDELGLRVLCTLSGDARFREVQTMHR 242 Query: 234 AKLNLLVCSRSMVTLARKMRDSFGIPYFEGSFCGSREIRFALRQLSFHFQNEGLDKQLNR 293 A++N++VCS++M+ +ARK+++ FG P+FEGSF G + ALR + ++ L + Sbjct: 243 AEVNMMVCSKAMLNVARKLQERFGTPWFEGSFYGITDTSQALRDFARLIGDDDLAARTEA 302 Query: 294 YMRREEERLHKDILRRYKPLKGKKVVLFTDGHESWIYISLLYELGIKIAAIGTNQNAQED 353 + REE R+ + + L GK+V+L+T G +SW IS L +LG+K+ A GT ++ +ED Sbjct: 303 LIAREEARIRAALEPWRERLAGKRVLLYTGGVKSWSVISALQDLGMKVVATGTKKSTEED 362 Query: 354 LSRIKERIHDDTLLIIQSDETRILQLYRERKGDLMIVSGRNSFVPLKEKIPFINIGEEQH 413 +RI+E + DD ++ + + +L+ E + D++I GRN + LK +IPF++I +E+ Sbjct: 363 KARIRELMGDDVKMLDEGNPRALLRTVEEYRADILIAGGRNMYTALKGRIPFLDINQERE 422 Query: 414 GGYAGYDGVRRFAQDVMDTLAQPVWTMIRRHSPWE 448 GYAGYDG+R + + TL PVW +R+ +PWE Sbjct: 423 FGYAGYDGMRELVRQLCLTLESPVWPAVRQPAPWE 457 >ref|ZP_00090766.1| COG2710: Nitrogenase molybdenum-iron protein, alpha and beta chains [Azotobacter vinelandii] Length = 490 Score = 357 bits (916), Expect = 4e-97, Method: Compositional matrix adjust. Identities = 171/456 (37%), Positives = 279/456 (61%), Gaps = 10/456 (2%) Query: 3 KGVPNLFIEPDCEHNRQPGKGCARPNPGEVTKGCVFQGSQAVLLPISDAAHLVHGTSGCL 62 K + L EP C HN++ GCA+P PG GC F G+Q LLP++D AH+VHG C Sbjct: 19 KDIAELLDEPACSHNKKEKSGCAKPKPGATDGGCSFDGAQIALLPVADVAHIVHGPIACA 78 Query: 63 QNSWGMLEAAMPEGPLPY-LRFSMGLTETDIILG-GQKKLLEAIQYIIEYYHPPCIFVYA 120 +SW GP Y + + LTE D+I+G +K+L AI+ +E Y PP +FVY Sbjct: 79 GSSWDN-RGTRSSGPDLYRIGMTTDLTENDVIMGRAEKRLFHAIRQAVESYSPPAVFVYN 137 Query: 121 TCITILTAEDLNAVCSKAESLWKLPVIPLHNSGFTGSGNMGSRQAGEALFEKVIGTCEPD 180 TC+ L +D++AVC A + PVIP+ ++GF G+ N+G+R AGEA+ + VIGT EPD Sbjct: 138 TCVPALIGDDVDAVCKAAAERFGTPVIPVDSAGFYGTKNLGNRIAGEAMLKYVIGTREPD 197 Query: 181 PRKLTP-------FDINLIGDYHFSKAGHEIEAMLSKIGIRVISRIAGECTFDELCSAHQ 233 P + D+NLIG+Y+ + + +L ++G+RV+ +AG+ + E+ + H+ Sbjct: 198 PLPVGSERPGIRVHDVNLIGEYNIAGEFWHVLPLLDELGLRVLCTLAGDARYREVQTMHR 257 Query: 234 AKLNLLVCSRSMVTLARKMRDSFGIPYFEGSFCGSREIRFALRQLSFHFQNEGLDKQLNR 293 A++N++VCS++M+ +ARK+++++G P+FEGSF G + ALR + + L + Sbjct: 258 AEVNMMVCSKAMLNVARKLQETYGTPWFEGSFYGITDTSQALRDFARLLDDPDLTARTEA 317 Query: 294 YMRREEERLHKDILRRYKPLKGKKVVLFTDGHESWIYISLLYELGIKIAAIGTNQNAQED 353 + REE ++ + L+GK+V+L+T G +SW +S L +LG+K+ A GT ++ +ED Sbjct: 318 LIAREEAKVRAALEPWRARLEGKRVLLYTGGVKSWSVVSALQDLGMKVVATGTKKSTEED 377 Query: 354 LSRIKERIHDDTLLIIQSDETRILQLYRERKGDLMIVSGRNSFVPLKEKIPFINIGEEQH 413 +RI+E + DD ++ + + +L+ E + D++I GRN + LK ++PF++I +E+ Sbjct: 378 KARIRELMGDDVKMLDEGNARVLLKTVDEYQADILIAGGRNMYTALKGRVPFLDINQERE 437 Query: 414 GGYAGYDGVRRFAQDVMDTLAQPVWTMIRRHSPWEV 449 GYAGYDG+ + + TL PVW +RR +PW++ Sbjct: 438 FGYAGYDGMLELVRQLCITLECPVWEAVRRPAPWDI 473 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.322 0.140 0.428 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 705,290,199 Number of extensions: 29854170 Number of successful extensions: 64646 Number of sequences better than 1.0e-05: 264 Number of HSP's gapped: 63894 Number of HSP's successfully gapped: 264 Length of query: 453 Length of database: 757,237,578 Length adjustment: 129 Effective length of query: 324 Effective length of database: 406,989,807 Effective search space: 131864697468 Effective search space used: 131864697468 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 128 (53.9 bits)