BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0579.0037.p (327 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_00077751.1| COG0596: Predicted hydrolases or acyltransfer... 201 2e-50 ref|NP_687697.1| protein of unknown function [Streptococcus agal... 161 2e-38 ref|NP_687696.1| protein of unknown function [Streptococcus agal... 152 1e-35 ref|NP_687698.1| conserved domain protein [Streptococcus agalact... 144 3e-33 ref|NP_832790.1| putative hydrolase [Bacillus cereus ATCC 14579]... 123 6e-27 >ref|ZP_00077751.1| COG0596: Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily) [Methanosarcina barkeri] Length = 329 Score = 201 bits (512), Expect = 2e-50, Method: Compositional matrix adjust. Identities = 114/316 (36%), Positives = 176/316 (55%), Gaps = 15/316 (4%) Query: 20 RNLWLKIFGGILGALVLFMGIVFIVNVISNGVEKKKIE-SYGQYVNVDGKKMNVLIQGSG 78 R L+ K GI AL++ + + N E K +E +YG V VDGK M + + GSG Sbjct: 7 RKLFKKALIGIPLALIIIIVLSATTNSFLCHQESKDLENAYGHRVQVDGKNMCIDVAGSG 66 Query: 79 EQTVVLLPGQGTPSPALDFKLLIDELSPDYKVVAIEPFGYGLSDETEKERTTENIISEIH 138 +Q VVLL G G+PSP LD + L L Y V+ E FGYGLSD+T +R+ ENI E+H Sbjct: 67 DQVVVLLSGWGSPSPVLDLRPLAANLDDKYTVITAEYFGYGLSDDTNSDRSVENITYELH 126 Query: 139 EAVQQLGIDHYVLMGHSITGLYGVSYVNAYPDEVLAFVGIDSSVPNQPGMDVKLPL---- 194 A++++G ++Y LM HSI+GLYG+ Y N YP+EV +FVGID+SV N+ ++ + Sbjct: 127 RALKEMGYNNYTLMAHSISGLYGLYYANMYPNEVKSFVGIDTSVANEDDLEASMQESLSY 186 Query: 195 -KSMQFLQKSGLMRLFQKVSGDPYKSLA------FDEHTKEQMRLISNQVAVNSTMMDEL 247 +++ L K G++RL + DP + E +E +R + ++ M+E+ Sbjct: 187 GNTLRTLNKLGIIRLLSVI--DPSSIIPEASGYQRSEEEQELLRKMYLNRLLSQNAMNEM 244 Query: 248 RHLGSNFKNGEQLTYPHELPLLLFVQANN-EDIKQWIPLHEEQVKHSAQGKMILMDGSHY 306 L N + +P +P+L F+ + N E W H EQ ++ + K+I+++GSHY Sbjct: 245 EMLEENCNKLSGMKFPDNVPVLFFISSENVETTSGWKEKHVEQFGNNGKNKLIVLNGSHY 304 Query: 307 LHHTKFKEIAEAFKAF 322 L++ EI FK + Sbjct: 305 LYNEYAPEICNTFKEW 320 >ref|NP_687697.1| protein of unknown function [Streptococcus agalactiae 2603V/R] gb|AAM99569.1| protein of unknown function [Streptococcus agalactiae 2603V/R] Length = 339 Score = 161 bits (408), Expect = 2e-38, Method: Compositional matrix adjust. Identities = 92/283 (32%), Positives = 148/283 (52%), Gaps = 26/283 (9%) Query: 54 KKIESYGQYVNV-DGKKMNVLIQGSGEQTVVLLPGQGTPSPALDFKLLIDELSPDYKVVA 112 K IE YGQ ++V DGKKMN+ G G+ +V+ PGQ SP +K L+D L YK+ Sbjct: 67 KPIEDYGQKIDVGDGKKMNIYTMGQGKIPLVVFPGQAEISPRYAYKNLLDRLGKKYKIYT 126 Query: 113 IEPFGYGLSDETEKERTTENIISEIHEAVQQLGIDHYVLMGHSITGLYGVSYVNAYPDEV 172 +EP GYGLSD T+K RT ENI EIH A++++G+ ++ LM HS+ G+YG++Y Y ++V Sbjct: 127 VEPLGYGLSDMTDKPRTIENITKEIHTALEKVGVKNFYLMAHSLGGIYGLNYAKTYSNDV 186 Query: 173 LAFVGIDSSVPNQPGMDVKLPLKSMQFLQKSGLMRLFQKVSGDPYKSLAFDEHTKEQMRL 232 F+G+D+S PN G + ++ + + D+ T EQ Sbjct: 187 KGFIGMDTSTPNMEG-------DGTTGESSASTLKWAESIP-------EVDKKTNEQYLS 232 Query: 233 ISNQVAVNSTMMDELRHLGSNFKNGEQLTYPHELPLLLFV-QANNEDI----------KQ 281 I + + N T D+ R + N E++ +P +P F+ + EDI K Sbjct: 233 IGAKKSGNKTERDKDRRVSDNLHKMEKVKFPKGMPAKYFLANESAEDIKMRKKIFPKLKS 292 Query: 282 WIPLHEEQVKHSAQGKMILMDGSHYLHHTKFKEIAEAFKAFMK 324 W H + + + +D SH ++HT++K +A+A +++ Sbjct: 293 WEQQHIDLSEDPKDVSVETLDASHLIYHTQYKHMAKAIDEWIQ 335 >ref|NP_687696.1| protein of unknown function [Streptococcus agalactiae 2603V/R] gb|AAM99568.1| protein of unknown function [Streptococcus agalactiae 2603V/R] Length = 343 Score = 152 bits (383), Expect = 1e-35, Method: Compositional matrix adjust. Identities = 91/292 (31%), Positives = 143/292 (48%), Gaps = 39/292 (13%) Query: 54 KKIESYGQYVNV-DGKKMNVLIQGSGEQTVVLLPGQGTPSPALDFKLLIDELSPDYKVVA 112 K ++ YG+ ++V DGKKMN+ G G+ +V +PGQ SP +K LI+ LS YK+ Sbjct: 72 KPVKDYGKKIDVGDGKKMNIYETGQGKIPIVFIPGQAEISPRYAYKNLIERLSKKYKIYT 131 Query: 113 IEPFGYGLSDETEKERTTENIISEIHEAVQQLGIDHYVLMGHSITGLYGVSYVNAYPDEV 172 +EP GYGLSD K RT ENI EIH + ++G+ ++ L HS+ G+Y ++Y YP+EV Sbjct: 132 VEPLGYGLSDIPTKPRTLENITKEIHTGLNKIGVKNFYLAAHSLGGMYSLNYAKNYPEEV 191 Query: 173 LAFVGIDSSVPNQPGMDVKLPLKSMQFLQKSGLMRLFQKVSGDP------YKSLAFDEHT 226 F+G+D+S P M QK DP K D+ T Sbjct: 192 RGFIGMDTSTP---------------------WMEGEQKTKYDPESAKWAMKLPDVDDKT 230 Query: 227 KEQMRLISNQVAVNSTMMDELRHLGSNFKNGEQLTYPHELPLLLFV-----------QAN 275 EQ I+ ++ N + DE LG N + + +P +P F+ QA+ Sbjct: 231 NEQYLSIAKKINGNKSQRDEDERLGDNLGKLKNVKFPKGMPTKYFLADQSVEGIKLRQAD 290 Query: 276 NEDIKQWIPLHEEQVKHSAQGKMILMDGSHYLHHTKFKEIAEAFKAFMKQIQ 327 + +K W H + + + + GSH + T+++ +A+A F++ Q Sbjct: 291 FKGLKNWDQQHIDLSEDPKDASVEIFKGSHNIFQTQYEHMAKAIDGFIQSHQ 342 >ref|NP_687698.1| conserved domain protein [Streptococcus agalactiae 2603V/R] gb|AAM99570.1| conserved domain protein [Streptococcus agalactiae 2603V/R] Length = 353 Score = 144 bits (363), Expect = 3e-33, Method: Compositional matrix adjust. Identities = 100/301 (33%), Positives = 148/301 (49%), Gaps = 33/301 (10%) Query: 42 FIVNVISNGVEKKKIESYGQYVNVDGKKMNVLIQGSGEQTVVLLPGQGTPSPALDFKLLI 101 FI N K I+ YGQ V V GKKMNV G G+ +V +PGQGT + + LI Sbjct: 70 FISNKQYQKYIKTPIKEYGQKVEVKGKKMNVYTVGEGKVPIVFIPGQGTVTAKHQYHNLI 129 Query: 102 DELSPDYKVVAIEPFGYGLSDETEKERTTENIISEIHEAVQQLGID-HYVLMGHSITGLY 160 LS +KVV +EPFG GLSD ++ R NI S+IHEA+Q++GI YV+ HSI G+Y Sbjct: 130 SNLSKTHKVVVVEPFGSGLSDVIDQPRNLANITSDIHEALQKVGITGKYVIASHSIGGVY 189 Query: 161 GVSYVNAYPDEVLAFVGIDSSVPNQPG---MDVKLPLKSMQFLQKSGLMRLFQKVSGDPY 217 + Y++ YP EVL +G+D+S P G +D P+ ++ L K KVS D Sbjct: 190 ALKYISTYPKEVLGLIGLDTSTPGMEGGKQVDFAAPV--LKELPK------IPKVSDD-- 239 Query: 218 KSLAFDEHTKEQMRLISNQVAVNSTMMDELRHLGSNFKNGEQLTYPHELPLL-------- 269 Q I +++ NS M +E ++ + P +P + Sbjct: 240 --------INAQFFAIGHKILNNSNMKEEAKNSSNMINESANYKIPKGIPAMYLLARESD 291 Query: 270 LFVQANNEDIK---QWIPLHEEQVKHSAQGKMILMDGSHYLHHTKFKEIAEAFKAFMKQI 326 L +Q + DI W H+E + + + +DG+H ++ T KE+ + FMK Sbjct: 292 LGLQLRSLDIPIKGSWQEQHKELSEDPKEVSIHTLDGTHQIYDTNLKEVIDYTNQFMKTF 351 Query: 327 Q 327 + Sbjct: 352 E 352 >ref|NP_832790.1| putative hydrolase [Bacillus cereus ATCC 14579] gb|AAP09991.1| putative hydrolase [Bacillus cereus ATCC 14579] Length = 328 Score = 123 bits (308), Expect = 6e-27, Method: Compositional matrix adjust. Identities = 82/260 (31%), Positives = 132/260 (50%), Gaps = 10/260 (3%) Query: 24 LKIFGGILGALVLF-MGIVFIVNVISNGVEKKKIESYGQYVNVDGKKMNVLIQGSGEQTV 82 +KI I +++ F G VFI + +E K + + G+ VN D KK+NV +GSGE T Sbjct: 8 VKIIFLICISMIFFGTGAVFIYHNCQLKMESKLMNNKGELVNFDNKKVNVYNEGSGEDTF 67 Query: 83 VLLPGQGTPSPALDFKLLIDELSPDYKVVAIEPFGYGLSDETEKERTTENIISEIHEAVQ 142 V + G G +P + K L + S + K+ IE GYG SD + +R + I+ + EA+ Sbjct: 68 VFMAGSGIAAPVYELKGLYSKFSKENKISVIERAGYGYSDVFQDDRDIDTILEQTREALI 127 Query: 143 QLGID-HYVLMGHSITGLYGVSYVNAYPDEVLAFVGIDSSVP-----NQPGMDVKLPLKS 196 + G Y+L+ HS++G+ + + YP EV + +D +P N+ GM L +K Sbjct: 128 RSGNKPPYILVPHSLSGIEAIYWAQKYPSEVKGIIALDIGLPKQYVTNKIGMVDSLKIKG 187 Query: 197 MQFLQKSGLMRLFQKVSGDP--YKSLAFDEHTKEQMRLISNQVAVNSTMMDELRHLGSNF 254 M L K G RL ++ +P + +E K+ + ++ + A N M EL +N Sbjct: 188 MNILTKIGFQRLAPSITYNPEVIRQSFLNEQEKDVYKALTYKRAFNDDMKQELLQSYNNG 247 Query: 255 KNGEQLTYPHELPLLLFVQA 274 K L+ P E P +LF+ A Sbjct: 248 KKSNSLSIPKETP-MLFIDA 266 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.317 0.136 0.386 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 495,664,184 Number of extensions: 21617942 Number of successful extensions: 69619 Number of sequences better than 1.0e-05: 44 Number of HSP's gapped: 69777 Number of HSP's successfully gapped: 44 Length of query: 327 Length of database: 757,237,578 Length adjustment: 126 Effective length of query: 201 Effective length of database: 415,135,104 Effective search space: 83442155904 Effective search space used: 83442155904 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 126 (53.1 bits)