BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0579.0024.p (433 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_00087966.1| COG0477: Permeases of the major facilitator s... 556 e-157 ref|ZP_00139973.1| COG0477: Permeases of the major facilitator s... 555 e-156 ref|NP_250952.1| probable 2-ketogluconate transporter [Pseudomon... 552 e-156 ref|ZP_00092807.1| COG0477: Permeases of the major facilitator s... 540 e-152 ref|NP_745517.1| 2-ketogluconate transporter, putative [Pseudomo... 536 e-151 >ref|ZP_00087966.1| COG0477: Permeases of the major facilitator superfamily [Pseudomonas fluorescens PfO-1] Length = 432 Score = 556 bits (1432), Expect = e-157, Method: Compositional matrix adjust. Identities = 275/421 (65%), Positives = 327/421 (77%), Gaps = 4/421 (0%) Query: 13 RWLRLMPIVFVTYSLAYLDRANYSFGAAAGMAKDLHISAGMSSXXXXXXXXXXXXXQVPG 72 RW +MPIVF+TYSLAYLDRANY F AA+GMA+DL I+ G+SS QVPG Sbjct: 10 RWWYIMPIVFITYSLAYLDRANYGFAAASGMAEDLMITPGLSSLLGALFFLGYFFFQVPG 69 Query: 73 AHYAENKSAKKLVFGSLILWGLLATATGIVHNVHFLIIIRFCLGVVESAVMPAMLVFLSH 132 A YA+ S KKL+F SLILWG LAT TG+V N ++LI+IRF LGVVE+AVMPAMLV+L H Sbjct: 70 AIYAQKHSVKKLIFVSLILWGGLATLTGVVSNAYWLIVIRFMLGVVEAAVMPAMLVYLCH 129 Query: 133 WFTKKERSRANTFLILGNPVTVLWMSIVSGYLVSAVGWRWMFIIEGLPPIIWAFFWWKLV 192 WFT+ ERSRANTFLILGNPVT+LWMS+VSGYLV WRWMFIIEGLP ++WAF WWKL Sbjct: 130 WFTRAERSRANTFLILGNPVTMLWMSVVSGYLVQHFSWRWMFIIEGLPAVLWAFIWWKLA 189 Query: 193 NDKPSETNWLTSQEKKELESALHEEQKGLKPVKNYGEAFRSPLVIRLCLQYFFWSIGVYG 252 +D+P++ WL+ QEK +LESAL EQ G+K VKNY EAFRSP VI L LQ+F WSIGVYG Sbjct: 190 DDRPAQAKWLSDQEKHDLESALAAEQVGIKAVKNYAEAFRSPKVIILALQFFCWSIGVYG 249 Query: 253 FVMWLPSIIKAAPDMSMVATGWLTSVPYVLAVIGMLTASYFSDKTQNRKAFVWPFLMVGA 312 FV+WLPSI+KA M M+ GWL+++PY+ AVIGML S+ SDK Q RK FVWP L++ + Sbjct: 250 FVLWLPSILKAGAQMDMIEAGWLSALPYLAAVIGMLLVSWGSDKLQKRKRFVWPPLLIAS 309 Query: 313 VAFYASYLLGADHFWISFILLIIAGGAMYAPYGPFFAIMPEILPRNVSGGAMALINSMGA 372 VAFY SY LGA+HFW S+ LL+IAG MYAPYGPFFAI+PEILP NV+GGAMALINSMGA Sbjct: 310 VAFYGSYALGAEHFWWSYTLLVIAGACMYAPYGPFFAIVPEILPANVAGGAMALINSMGA 369 Query: 373 LGSFAGSYIVGYLNSFTGGFGASYIFMAGSLLLSAILTMTVGRSRKPAESSTEGKLEVST 432 LGSF GSY+VGYLNS TG GASY+ M+G+L+LS +LT+ + KP S V+ Sbjct: 370 LGSFGGSYLVGYLNSSTGSPGASYLLMSGALMLSVVLTIFL----KPGASDRVTAKRVAP 425 Query: 433 R 433 R Sbjct: 426 R 426 >ref|ZP_00139973.1| COG0477: Permeases of the major facilitator superfamily [Pseudomonas aeruginosa UCBPP-PA14] Length = 600 Score = 555 bits (1429), Expect = e-156, Method: Compositional matrix adjust. Identities = 267/403 (66%), Positives = 324/403 (80%) Query: 9 FVASRWLRLMPIVFVTYSLAYLDRANYSFGAAAGMAKDLHISAGMSSXXXXXXXXXXXXX 68 +SRW +MPIVFVTYSLAYLDRANY F AA+GMA DL I+ G+SS Sbjct: 171 LASSRWWYIMPIVFVTYSLAYLDRANYGFAAASGMADDLRITPGLSSLLGALFFLGYFFF 230 Query: 69 QVPGAHYAENKSAKKLVFGSLILWGLLATATGIVHNVHFLIIIRFCLGVVESAVMPAMLV 128 QVPGA YAE +S KKL+F SLILWG LAT TG+V NV+ LI IRF LGVVE+AVMPAMLV Sbjct: 231 QVPGAIYAEKRSVKKLIFVSLILWGGLATLTGMVANVYLLIGIRFLLGVVEAAVMPAMLV 290 Query: 129 FLSHWFTKKERSRANTFLILGNPVTVLWMSIVSGYLVSAVGWRWMFIIEGLPPIIWAFFW 188 +L HWFT+ ERSRANTFLILGNPVT+LWMS+VSGYLV WRWMFIIEGLP +IWAF W Sbjct: 291 YLCHWFTRAERSRANTFLILGNPVTILWMSVVSGYLVEHFSWRWMFIIEGLPAVIWAFIW 350 Query: 189 WKLVNDKPSETNWLTSQEKKELESALHEEQKGLKPVKNYGEAFRSPLVIRLCLQYFFWSI 248 W+LV+D+P + WL+ EK++LE AL EQ+G+KPVKNY EAFRSP VI L LQYF WSI Sbjct: 351 WRLVDDRPRQAAWLSGSEKRDLEDALAAEQQGIKPVKNYREAFRSPKVIVLSLQYFCWSI 410 Query: 249 GVYGFVMWLPSIIKAAPDMSMVATGWLTSVPYVLAVIGMLTASYFSDKTQNRKAFVWPFL 308 GVYGFV+WLPSI+K ++ ++ GWL+++PY+ AVI ML S+ SD+ Q RK FVWP L Sbjct: 411 GVYGFVLWLPSILKHGANIDIIEAGWLSALPYLAAVIAMLGVSWASDRLQKRKRFVWPPL 470 Query: 309 MVGAVAFYASYLLGADHFWISFILLIIAGGAMYAPYGPFFAIMPEILPRNVSGGAMALIN 368 ++ ++AFY SY LG++HFW+S+ LL++AG MYAPYGPFFAI+PE+LP NV+GGAMALIN Sbjct: 471 LIASIAFYGSYALGSEHFWLSYALLVLAGACMYAPYGPFFAIVPEVLPANVAGGAMALIN 530 Query: 369 SMGALGSFAGSYIVGYLNSFTGGFGASYIFMAGSLLLSAILTM 411 SMGALGSF+GS++VGYLN TGG GASY+FM+G+LLLS +LT+ Sbjct: 531 SMGALGSFSGSWLVGYLNGITGGPGASYLFMSGALLLSVLLTV 573 >ref|NP_250952.1| probable 2-ketogluconate transporter [Pseudomonas aeruginosa PA01] ref|ZP_00139975.1| COG0477: Permeases of the major facilitator superfamily [Pseudomonas aeruginosa UCBPP-PA14] pir||E83362 probable 2-ketogluconate transporter PA2262 [imported] - Pseudomonas aeruginosa (strain PAO1) gb|AAG05650.1| probable 2-ketogluconate transporter [Pseudomonas aeruginosa PAO1] gb|AAK37649.1| KguT [Pseudomonas aeruginosa] Length = 435 Score = 552 bits (1423), Expect = e-156, Method: Compositional matrix adjust. Identities = 267/401 (66%), Positives = 324/401 (80%) Query: 11 ASRWLRLMPIVFVTYSLAYLDRANYSFGAAAGMAKDLHISAGMSSXXXXXXXXXXXXXQV 70 +SRW +MPIVFVTYSLAYLDRANY F AA+GMA DL I+ G+SS QV Sbjct: 8 SSRWWYIMPIVFVTYSLAYLDRANYGFAAASGMADDLRITPGLSSLLGALFFLGYFFFQV 67 Query: 71 PGAHYAENKSAKKLVFGSLILWGLLATATGIVHNVHFLIIIRFCLGVVESAVMPAMLVFL 130 PGA YAE +S KKL+F SLILWG LAT TG+V NV+ LI IRF LGVVE+AVMPAMLV+L Sbjct: 68 PGAIYAEKRSVKKLIFVSLILWGGLATLTGMVANVYLLIGIRFLLGVVEAAVMPAMLVYL 127 Query: 131 SHWFTKKERSRANTFLILGNPVTVLWMSIVSGYLVSAVGWRWMFIIEGLPPIIWAFFWWK 190 HWFT+ ERSRANTFLILGNPVT+LWMS+VSGYLV WRWMFIIEGLP +IWAF WW+ Sbjct: 128 CHWFTRAERSRANTFLILGNPVTILWMSVVSGYLVEHFSWRWMFIIEGLPAVIWAFIWWR 187 Query: 191 LVNDKPSETNWLTSQEKKELESALHEEQKGLKPVKNYGEAFRSPLVIRLCLQYFFWSIGV 250 LV+D+P + WL+ EK++LE AL EQ+G+KPVKNY EAFRSP VI L LQYF WSIGV Sbjct: 188 LVDDRPRQAAWLSGSEKRDLEDALAAEQQGIKPVKNYREAFRSPKVIVLSLQYFCWSIGV 247 Query: 251 YGFVMWLPSIIKAAPDMSMVATGWLTSVPYVLAVIGMLTASYFSDKTQNRKAFVWPFLMV 310 YGFV+WLPSI+K ++ ++ GWL+++PY+ AVI ML S+ SD+ Q RK FVWP L++ Sbjct: 248 YGFVLWLPSILKHGANIDIIEAGWLSALPYLAAVIAMLGVSWASDRLQKRKRFVWPPLLI 307 Query: 311 GAVAFYASYLLGADHFWISFILLIIAGGAMYAPYGPFFAIMPEILPRNVSGGAMALINSM 370 ++AFY SY LG++HFW+S+ LL++AG MYAPYGPFFAI+PE+LP NV+GGAMALINSM Sbjct: 308 ASIAFYGSYALGSEHFWLSYALLVLAGACMYAPYGPFFAIVPEVLPANVAGGAMALINSM 367 Query: 371 GALGSFAGSYIVGYLNSFTGGFGASYIFMAGSLLLSAILTM 411 GALGSF+GS++VGYLN TGG GASY+FM+G+LLLS +LT+ Sbjct: 368 GALGSFSGSWLVGYLNGITGGPGASYLFMSGALLLSVLLTV 408 >ref|ZP_00092807.1| COG0477: Permeases of the major facilitator superfamily [Azotobacter vinelandii] Length = 614 Score = 540 bits (1390), Expect = e-152, Method: Compositional matrix adjust. Identities = 255/394 (64%), Positives = 316/394 (80%) Query: 9 FVASRWLRLMPIVFVTYSLAYLDRANYSFGAAAGMAKDLHISAGMSSXXXXXXXXXXXXX 68 + RW L+P++F+TYSLAYLDRANY F AA+GMA DL+I+ G+SS Sbjct: 188 LASRRWWYLIPVIFITYSLAYLDRANYGFAAASGMAADLNITPGLSSLLGALFFLGYFFF 247 Query: 69 QVPGAHYAENKSAKKLVFGSLILWGLLATATGIVHNVHFLIIIRFCLGVVESAVMPAMLV 128 QVPGA YAE +S KKL+F SLILWG LAT TG++ NV+ LI IRF LGVVE+AVMPAML+ Sbjct: 248 QVPGAIYAERRSVKKLIFVSLILWGGLATLTGVIPNVYLLIAIRFLLGVVEAAVMPAMLI 307 Query: 129 FLSHWFTKKERSRANTFLILGNPVTVLWMSIVSGYLVSAVGWRWMFIIEGLPPIIWAFFW 188 +L HWFT+ ERSRANTFLILGNPVT+LWMS+VSGYL+ WRWMFIIEGLP ++WAF W Sbjct: 308 YLCHWFTRAERSRANTFLILGNPVTILWMSVVSGYLIEHFDWRWMFIIEGLPAVLWAFIW 367 Query: 189 WKLVNDKPSETNWLTSQEKKELESALHEEQKGLKPVKNYGEAFRSPLVIRLCLQYFFWSI 248 W+L +D+P++ WL ++EK+EL+ AL EQ+GLKPVKNY EAFRSP VI L +QYF WSI Sbjct: 368 WRLADDRPADAKWLDAREKEELQQALAAEQQGLKPVKNYREAFRSPKVILLSVQYFCWSI 427 Query: 249 GVYGFVMWLPSIIKAAPDMSMVATGWLTSVPYVLAVIGMLTASYFSDKTQNRKAFVWPFL 308 GVYG V+WLPSIIK A + +V GWL+S+PY+ AVIGML S+ SD+ Q RK FVWP L Sbjct: 428 GVYGLVLWLPSIIKDAAQLDIVQAGWLSSLPYLAAVIGMLGVSWASDRFQQRKCFVWPPL 487 Query: 309 MVGAVAFYASYLLGADHFWISFILLIIAGGAMYAPYGPFFAIMPEILPRNVSGGAMALIN 368 ++ ++AFY SY LG++HFW+S+ LL++AGG MYAPYGPFFAI+PE+LP NV+GGAMALIN Sbjct: 488 LIASLAFYGSYALGSEHFWLSYALLVLAGGCMYAPYGPFFAIVPEVLPVNVAGGAMALIN 547 Query: 369 SMGALGSFAGSYIVGYLNSFTGGFGASYIFMAGS 402 SMGALGSFAGS++VGYLN TG GAS++ M+G+ Sbjct: 548 SMGALGSFAGSWLVGYLNGATGNPGASFLLMSGA 581 >ref|NP_745517.1| 2-ketogluconate transporter, putative [Pseudomonas putida KT2440] gb|AAN68981.1| 2-ketogluconate transporter, putative [Pseudomonas putida KT2440] Length = 430 Score = 536 bits (1381), Expect = e-151, Method: Compositional matrix adjust. Identities = 266/413 (64%), Positives = 322/413 (77%) Query: 13 RWLRLMPIVFVTYSLAYLDRANYSFGAAAGMAKDLHISAGMSSXXXXXXXXXXXXXQVPG 72 RW ++PIVFVTYSLAYLDRANY F AA+GMA+DLHI+ +SS QVPG Sbjct: 10 RWWYIIPIVFVTYSLAYLDRANYGFAAASGMAEDLHITPVLSSLLGALFFLGYFFFQVPG 69 Query: 73 AHYAENKSAKKLVFGSLILWGLLATATGIVHNVHFLIIIRFCLGVVESAVMPAMLVFLSH 132 A YAE +S KKL+F LILWG LAT TG+V NV+ LI IRF LGVVE+AVMPAMLV+L H Sbjct: 70 AIYAEKRSVKKLIFVCLILWGGLATLTGMVSNVYMLIGIRFLLGVVEAAVMPAMLVYLCH 129 Query: 133 WFTKKERSRANTFLILGNPVTVLWMSIVSGYLVSAVGWRWMFIIEGLPPIIWAFFWWKLV 192 WFT+ ERSRANTFL+LGNPVT+LWMS+VSGYL+ WRWMFIIEGLP +IWAFFWW+LV Sbjct: 130 WFTRAERSRANTFLMLGNPVTILWMSVVSGYLIKHYDWRWMFIIEGLPAVIWAFFWWRLV 189 Query: 193 NDKPSETNWLTSQEKKELESALHEEQKGLKPVKNYGEAFRSPLVIRLCLQYFFWSIGVYG 252 +D+P++ WL+ QEK L AL EQ+G+KPVKNY EAFRSP VI L LQYF WSIGVYG Sbjct: 190 DDRPAQVAWLSEQEKAALAQALAAEQQGIKPVKNYREAFRSPQVIILALQYFCWSIGVYG 249 Query: 253 FVMWLPSIIKAAPDMSMVATGWLTSVPYVLAVIGMLTASYFSDKTQNRKAFVWPFLMVGA 312 FV+WLPSI+K A ++ +V GWL SVPY+ AV+ M+ S+ SD+ Q RK FVWP L++ A Sbjct: 250 FVLWLPSILKQAANVDIVQAGWLASVPYLAAVLAMVGVSWASDRLQKRKRFVWPPLLIAA 309 Query: 313 VAFYASYLLGADHFWISFILLIIAGGAMYAPYGPFFAIMPEILPRNVSGGAMALINSMGA 372 +AFY SY LG++HFW+S+ LL++AG MYAPYGPFFAI+PEILP NV+GGAMALINSMGA Sbjct: 310 LAFYGSYALGSEHFWLSYALLVVAGACMYAPYGPFFAIVPEILPANVAGGAMALINSMGA 369 Query: 373 LGSFAGSYIVGYLNSFTGGFGASYIFMAGSLLLSAILTMTVGRSRKPAESSTE 425 LGSF GS++VGYLN TGG GASY+FM G+LL + LT + S+ + + Sbjct: 370 LGSFGGSWLVGYLNGATGGPGASYLFMCGALLTAVALTAALNTSQTSGRAKRD 422 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.325 0.138 0.435 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 640,055,800 Number of extensions: 26444083 Number of successful extensions: 107207 Number of sequences better than 1.0e-05: 1065 Number of HSP's gapped: 105818 Number of HSP's successfully gapped: 1067 Length of query: 433 Length of database: 757,237,578 Length adjustment: 129 Effective length of query: 304 Effective length of database: 406,989,807 Effective search space: 123724901328 Effective search space used: 123724901328 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 127 (53.5 bits)