BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0576.0005.p (482 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_391805.1| beta-glucosidase [Bacillus subtilis] >gi|743544... 661 0.0 sp|P40740|BGL1_BACSU Beta-glucosidase (Gentiobiase) (Cellobiase)... 660 0.0 emb|CAA84287.1| beta-glucosidase [Bacillus subtilis] 658 0.0 ref|NP_241462.1| beta-glucosidase [Bacillus halodurans] >gi|2528... 618 e-175 ref|NP_463551.1| beta-glucosidase [Listeria monocytogenes EGD-e]... 581 e-164 >ref|NP_391805.1| beta-glucosidase [Bacillus subtilis] pir||H69593 6-phospho-beta-glucosidase (EC 3.2.1.86) bglH [similarity] - Bacillus subtilis dbj|BAA06653.1| hypothetical protein [Bacillus subtilis] dbj|BAA06257.1| hypothetical 54.6-kDa protein [Bacillus subtilis] Length = 481 Score = 661 bits (1705), Expect = 0.0, Method: Compositional matrix adjust. Identities = 312/471 (66%), Positives = 364/471 (77%) Query: 11 LTLHSNTHSFPENFLWGGAVAANQVEGAFLEDGKGLSTADVLPRGITGPIMEIPEGEYPS 70 + + SN FPE FLWGGAVAANQVEGA+ E GKGLSTADV P GI P E Sbjct: 11 INMSSNEKRFPEGFLWGGAVAANQVEGAYNEGGKGLSTADVSPNGIMSPFDESMTSLNLY 70 Query: 71 HEAIDFYHRYKEDIAMFAEMGFRCFRTSIAWTRIFPEGDEEIPNEEGIRFYEQLIDELLK 130 H IDFYHRYKEDIA+FAEMGF+ FRTSIAWTRIFP GDEE PNEEG+RFY+ L DELLK Sbjct: 71 HNGIDFYHRYKEDIALFAEMGFKAFRTSIAWTRIFPNGDEEEPNEEGLRFYDDLFDELLK 130 Query: 131 YNIEPVITISHYEMPLGLAKKYGGWRSRKVIECYERYAKVLFERFKGKVKYWMTFNEINI 190 ++IEPV+TISHYEMPLGL K YGGW++RKVIE YERYAK +F+R++ KVKYWMTFNEIN+ Sbjct: 131 HHIEPVVTISHYEMPLGLVKNYGGWKNRKVIEFYERYAKTVFKRYQHKVKYWMTFNEINV 190 Query: 191 LAHYPFTGGGIILRNGENQKQVQYQAAHHQFVASALAVKACHEIIPDAQIGCMLAYIPAY 250 + H PFTGGG++ GEN+ YQAAHHQFVASALAVKA H+IIPD++IGCM+A Y Sbjct: 191 VLHAPFTGGGLVFEEGENKLNAMYQAAHHQFVASALAVKAGHDIIPDSKIGCMIAATTTY 250 Query: 251 PLTSAPEDVFAALQGEREILMFGDVQAIGGYPSYAAQFFKENDVVLEKESGDDLILKSYP 310 P+TS PEDVFAA++ ER+ L F DVQA G YP Y ++ EN++ +E GD+ +LK + Sbjct: 251 PMTSKPEDVFAAMENERKTLFFSDVQARGAYPGYMKRYLAENNIEIEMAEGDEELLKEHT 310 Query: 311 VDFIGFSYYMSRAVSANPEGKETTAGNLFGGVKNPYLPSSEWGWQIDPLGLRIALNLLYD 370 VD+IGFSYYMS A S +PE + GNL GGVKNPYL SSEWGWQIDP GLRI LN LYD Sbjct: 311 VDYIGFSYYMSMAASTDPEELAKSGGNLLGGVKNPYLKSSEWGWQIDPKGLRITLNTLYD 370 Query: 371 RYQKPLFIVENGLGAADVLEAXXXXXXXXXXXXLREHLSQVSKAIQDGVDLIGYTSWGPI 430 RYQKPLFIVENGLGA D +E LR+HL + +AI DGV+LIGYTSWGPI Sbjct: 371 RYQKPLFIVENGLGAVDKVEEDGTIQDDYRINYLRDHLIEAREAIADGVELIGYTSWGPI 430 Query: 431 DLISASTGEMKKRYGYIYVDKNNAGEGTLRRIKKKSFAWYKKVIASNGSDL 481 DL+SAST EMKKRYG+IYVD++N G GT RIKKKSF WY++VIA+NG L Sbjct: 431 DLVSASTAEMKKRYGFIYVDRDNEGNGTFNRIKKKSFNWYQQVIATNGESL 481 >sp|P40740|BGL1_BACSU Beta-glucosidase (Gentiobiase) (Cellobiase) (Beta-D-glucoside glucohydrolase) (Amygdalase) emb|CAB15962.2| beta-glucosidase [Bacillus subtilis subsp. subtilis str. 168] Length = 469 Score = 660 bits (1702), Expect = 0.0, Method: Compositional matrix adjust. Identities = 312/469 (66%), Positives = 363/469 (77%) Query: 13 LHSNTHSFPENFLWGGAVAANQVEGAFLEDGKGLSTADVLPRGITGPIMEIPEGEYPSHE 72 + SN FPE FLWGGAVAANQVEGA+ E GKGLSTADV P GI P E H Sbjct: 1 MSSNEKRFPEGFLWGGAVAANQVEGAYNEGGKGLSTADVSPNGIMSPFDESMTSLNLYHN 60 Query: 73 AIDFYHRYKEDIAMFAEMGFRCFRTSIAWTRIFPEGDEEIPNEEGIRFYEQLIDELLKYN 132 IDFYHRYKEDIA+FAEMGF+ FRTSIAWTRIFP GDEE PNEEG+RFY+ L DELLK++ Sbjct: 61 GIDFYHRYKEDIALFAEMGFKAFRTSIAWTRIFPNGDEEEPNEEGLRFYDDLFDELLKHH 120 Query: 133 IEPVITISHYEMPLGLAKKYGGWRSRKVIECYERYAKVLFERFKGKVKYWMTFNEINILA 192 IEPV+TISHYEMPLGL K YGGW++RKVIE YERYAK +F+R++ KVKYWMTFNEIN++ Sbjct: 121 IEPVVTISHYEMPLGLVKNYGGWKNRKVIEFYERYAKTVFKRYQHKVKYWMTFNEINVVL 180 Query: 193 HYPFTGGGIILRNGENQKQVQYQAAHHQFVASALAVKACHEIIPDAQIGCMLAYIPAYPL 252 H PFTGGG++ GEN+ YQAAHHQFVASALAVKA H+IIPD++IGCM+A YP+ Sbjct: 181 HAPFTGGGLVFEEGENKLNAMYQAAHHQFVASALAVKAGHDIIPDSKIGCMIAATTTYPM 240 Query: 253 TSAPEDVFAALQGEREILMFGDVQAIGGYPSYAAQFFKENDVVLEKESGDDLILKSYPVD 312 TS PEDVFAA++ ER+ L F DVQA G YP Y ++ EN++ +E GD+ +LK + VD Sbjct: 241 TSKPEDVFAAMENERKTLFFSDVQARGAYPGYMKRYLAENNIEIEMAEGDEELLKEHTVD 300 Query: 313 FIGFSYYMSRAVSANPEGKETTAGNLFGGVKNPYLPSSEWGWQIDPLGLRIALNLLYDRY 372 +IGFSYYMS A S +PE + GNL GGVKNPYL SSEWGWQIDP GLRI LN LYDRY Sbjct: 301 YIGFSYYMSMAASTDPEELAKSGGNLLGGVKNPYLKSSEWGWQIDPKGLRITLNTLYDRY 360 Query: 373 QKPLFIVENGLGAADVLEAXXXXXXXXXXXXLREHLSQVSKAIQDGVDLIGYTSWGPIDL 432 QKPLFIVENGLGA D +E LR+HL + +AI DGV+LIGYTSWGPIDL Sbjct: 361 QKPLFIVENGLGAVDKVEEDGTIQDDYRINYLRDHLIEAREAIADGVELIGYTSWGPIDL 420 Query: 433 ISASTGEMKKRYGYIYVDKNNAGEGTLRRIKKKSFAWYKKVIASNGSDL 481 +SAST EMKKRYG+IYVD++N G GT RIKKKSF WY++VIA+NG L Sbjct: 421 VSASTAEMKKRYGFIYVDRDNEGNGTFNRIKKKSFNWYQQVIATNGESL 469 >emb|CAA84287.1| beta-glucosidase [Bacillus subtilis] Length = 469 Score = 658 bits (1697), Expect = 0.0, Method: Compositional matrix adjust. Identities = 311/469 (66%), Positives = 362/469 (77%) Query: 13 LHSNTHSFPENFLWGGAVAANQVEGAFLEDGKGLSTADVLPRGITGPIMEIPEGEYPSHE 72 + SN FPE FLWGGAVAANQVEGA+ E GKGLSTADV P GI P E H Sbjct: 1 MSSNEKRFPEGFLWGGAVAANQVEGAYNEGGKGLSTADVSPNGIMSPFDESMTSLNLYHN 60 Query: 73 AIDFYHRYKEDIAMFAEMGFRCFRTSIAWTRIFPEGDEEIPNEEGIRFYEQLIDELLKYN 132 IDFYHRYKEDIA+FAEMGF+ FRTSIAWTRIFP GDEE PNEEG+RFY+ L DELLK++ Sbjct: 61 GIDFYHRYKEDIALFAEMGFKAFRTSIAWTRIFPNGDEEEPNEEGLRFYDDLFDELLKHH 120 Query: 133 IEPVITISHYEMPLGLAKKYGGWRSRKVIECYERYAKVLFERFKGKVKYWMTFNEINILA 192 IEPV+TISHYEMPLGL K YGGW++RKVIE YERYAK +F+R++ KVKYWMTFNEIN++ Sbjct: 121 IEPVVTISHYEMPLGLVKNYGGWKNRKVIEFYERYAKTVFKRYQHKVKYWMTFNEINVVL 180 Query: 193 HYPFTGGGIILRNGENQKQVQYQAAHHQFVASALAVKACHEIIPDAQIGCMLAYIPAYPL 252 H PFTGGG++ GEN+ YQAAHHQFVASALAVKA H+IIPD++IGCM+A YP+ Sbjct: 181 HAPFTGGGLVFEEGENKLNAMYQAAHHQFVASALAVKAGHDIIPDSKIGCMIAATTTYPM 240 Query: 253 TSAPEDVFAALQGEREILMFGDVQAIGGYPSYAAQFFKENDVVLEKESGDDLILKSYPVD 312 TS PEDVFAA++ ER+ L F DVQA G YP Y ++ EN++ +E GD+ +LK + VD Sbjct: 241 TSKPEDVFAAMENERKTLFFSDVQARGAYPGYMKRYLAENNIEIEMAEGDEELLKEHTVD 300 Query: 313 FIGFSYYMSRAVSANPEGKETTAGNLFGGVKNPYLPSSEWGWQIDPLGLRIALNLLYDRY 372 +IGFSYYMS A S +PE + GNL GGVKNPYL SSEWGWQIDP GLRI LN LYDRY Sbjct: 301 YIGFSYYMSMAASTDPEELAKSGGNLLGGVKNPYLKSSEWGWQIDPKGLRITLNTLYDRY 360 Query: 373 QKPLFIVENGLGAADVLEAXXXXXXXXXXXXLREHLSQVSKAIQDGVDLIGYTSWGPIDL 432 QKPLFIVENGLGA D +E LR+HL + + I DGV+LIGYTSWGPIDL Sbjct: 361 QKPLFIVENGLGAVDKVEEDGTIQDDYRINYLRDHLIEARERIADGVELIGYTSWGPIDL 420 Query: 433 ISASTGEMKKRYGYIYVDKNNAGEGTLRRIKKKSFAWYKKVIASNGSDL 481 +SAST EMKKRYG+IYVD++N G GT RIKKKSF WY++VIA+NG L Sbjct: 421 VSASTAEMKKRYGFIYVDRDNEGNGTFNRIKKKSFNWYQQVIATNGESL 469 >ref|NP_241462.1| beta-glucosidase [Bacillus halodurans] pir||D83724 beta-glucosidase gblH [imported] - Bacillus halodurans (strain C-125) dbj|BAB04315.1| beta-glucosidase [Bacillus halodurans] Length = 477 Score = 618 bits (1593), Expect = e-175, Method: Compositional matrix adjust. Identities = 286/473 (60%), Positives = 365/473 (77%), Gaps = 10/473 (2%) Query: 16 NTHSFPENFLWGGAVAANQVEGAFLEDGKGLSTADVLPRG------ITGPIMEIP--EGE 67 + HSFPE FLWGGA+AANQ EGA+LEDGKGL+ D+LP G + G + EGE Sbjct: 2 SKHSFPEGFLWGGAIAANQAEGAYLEDGKGLTLVDLLPTGQKRWDVMLGKLDSFTPLEGE 61 Query: 68 -YPSHEAIDFYHRYKEDIAMFAEMGFRCFRTSIAWTRIFPEGDEEIPNEEGIRFYEQLID 126 YPSHEAIDFYHRY+ED+A+ AEMGF+ FR SI+W RIFP GD+ PNE+G++FY+ L D Sbjct: 62 FYPSHEAIDFYHRYQEDVALLAEMGFKAFRLSISWARIFPNGDDPEPNEKGLQFYDDLFD 121 Query: 127 ELLKYNIEPVITISHYEMPLGLAKKYGGWRSRKVIECYERYAKVLFERFKGKVKYWMTFN 186 ELLKYNI+PV+T++H+++P+ L + YGGWRSRK++ +E YAK + R+K KVKYWMTFN Sbjct: 122 ELLKYNIQPVVTMAHFDVPVHLIESYGGWRSRKLVGLFETYAKTILSRYKDKVKYWMTFN 181 Query: 187 EINILAHYPFTGGGIILRNGENQKQVQYQAAHHQFVASALAVKACHEIIPDAQIGCMLAY 246 EIN+L H PF G G++ + GEN+KQ+QYQAAHHQ VAS+LAVKACHE+IPDA+IGCMLA Sbjct: 182 EINMLLHLPFVGAGLVFKEGENKKQIQYQAAHHQLVASSLAVKACHELIPDAKIGCMLAA 241 Query: 247 IPAYPLTSAPEDVFAALQGEREILMFGDVQAIGGYPSYAAQFFKENDVVLEKESGDDLIL 306 YP T P DVF A+ +RE F DVQ+ G YP YA +FF+++++V++ E GD+ +L Sbjct: 242 GATYPYTCHPNDVFEAMTKDRESFFFIDVQSRGEYPGYAKRFFRDHNIVIDMEEGDEALL 301 Query: 307 KSYPVDFIGFSYYMSRAVSANPEG-KETTAGNLFGGVKNPYLPSSEWGWQIDPLGLRIAL 365 K++ VD+IGFSYY SR S +PE K T+GN+FG V+NPYL SEWGW IDP G R Sbjct: 302 KNHTVDYIGFSYYASRVTSTDPEVLKNITSGNVFGSVENPYLEKSEWGWTIDPKGFRTTA 361 Query: 366 NLLYDRYQKPLFIVENGLGAADVLEAXXXXXXXXXXXXLREHLSQVSKAIQDGVDLIGYT 425 N LYDRYQKPLF+VENGLGA D + LR+H++++++AI DGVD+IGYT Sbjct: 362 NQLYDRYQKPLFVVENGLGAVDDVTLEGEINDEYRIDYLRKHIAEMAEAIADGVDIIGYT 421 Query: 426 SWGPIDLISASTGEMKKRYGYIYVDKNNAGEGTLRRIKKKSFAWYKKVIASNG 478 SWGPID++SASTGEMKKRYGYIYVD++N G+G+L+R+KKKSF WYKKVI +NG Sbjct: 422 SWGPIDVVSASTGEMKKRYGYIYVDRDNEGKGSLKRMKKKSFHWYKKVIETNG 474 >ref|NP_463551.1| beta-glucosidase [Listeria monocytogenes EGD-e] pir||AC1077 beta-glucosidase [imported] - Listeria monocytogenes (strain EGD-e) emb|CAC98233.1| beta-glucosidase [Listeria monocytogenes] Length = 477 Score = 581 bits (1497), Expect = e-164, Method: Compositional matrix adjust. Identities = 272/472 (57%), Positives = 347/472 (73%), Gaps = 9/472 (1%) Query: 20 FPENFLWGGAVAANQVEGAFLEDGKGLSTADVLPRGITG---------PIMEIPEGEYPS 70 FP++FLWGGAVAANQ EGA+LEDGKGLS D+LP G + G YPS Sbjct: 6 FPKDFLWGGAVAANQCEGAYLEDGKGLSLVDILPTVEDGRWEALFNPSKALATDYGFYPS 65 Query: 71 HEAIDFYHRYKEDIAMFAEMGFRCFRTSIAWTRIFPEGDEEIPNEEGIRFYEQLIDELLK 130 HE+IDFYHRYKEDI + AEMGF+CFR SI+W RIFP GDE PNE+G+ FY+ + DE K Sbjct: 66 HESIDFYHRYKEDIKLMAEMGFKCFRMSISWPRIFPNGDETTPNEKGLAFYDAVFDECHK 125 Query: 131 YNIEPVITISHYEMPLGLAKKYGGWRSRKVIECYERYAKVLFERFKGKVKYWMTFNEINI 190 Y IEPV+TI+H++ PL + KKYGGW++RK I+ Y + + +F R+K KVKYWMTFNEIN+ Sbjct: 126 YGIEPVVTINHFDTPLEVFKKYGGWKNRKCIDFYLNFCEAIFTRYKDKVKYWMTFNEINM 185 Query: 191 LAHYPFTGGGIILRNGENQKQVQYQAAHHQFVASALAVKACHEIIPDAQIGCMLAYIPAY 250 + H P+ GGG+ + +N ++V+YQAAHHQ VASALA K HEI P+ QIGCMLA Y Sbjct: 186 ILHLPYIGGGLDVTKEDNPEEVKYQAAHHQLVASALATKLGHEINPENQIGCMLAAGNTY 245 Query: 251 PLTSAPEDVFAALQGEREILMFGDVQAIGGYPSYAAQFFKENDVVLEKESGDDLILKSYP 310 P+T P+DV+ +++ +RE F DVQA G YPSY +FFKE+++ ++ E GD L+ + Sbjct: 246 PMTCNPKDVWKSIEADREGYFFIDVQARGYYPSYTKRFFKEHNINIKMEDGDLDALRDHT 305 Query: 311 VDFIGFSYYMSRAVSANPEGKETTAGNLFGGVKNPYLPSSEWGWQIDPLGLRIALNLLYD 370 VD++ FSYY SR SA+PE + T GN+F +KNPYL +SEWGWQIDPLGLRI +N +YD Sbjct: 306 VDYVAFSYYSSRLTSADPEKNKETEGNVFATLKNPYLKASEWGWQIDPLGLRITMNTIYD 365 Query: 371 RYQKPLFIVENGLGAADVLEAXXXXXXXXXXXXLREHLSQVSKAIQDGVDLIGYTSWGPI 430 RYQKPLFIVENGLGA D +E +REH+ ++ +AI+DGV+L+GYT WG I Sbjct: 366 RYQKPLFIVENGLGAVDTVEEDGSITDDYRIDYMREHVREMGEAIEDGVELLGYTPWGCI 425 Query: 431 DLISASTGEMKKRYGYIYVDKNNAGEGTLRRIKKKSFAWYKKVIASNGSDLD 482 DL+SA +GEMKKRYG+IYVD++N G GTL R KKKSF WYKKVI +NG D+D Sbjct: 426 DLVSAGSGEMKKRYGFIYVDRDNKGNGTLNRSKKKSFDWYKKVIETNGKDID 477 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.319 0.139 0.421 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 747,034,098 Number of extensions: 32709243 Number of successful extensions: 68170 Number of sequences better than 1.0e-05: 695 Number of HSP's gapped: 65696 Number of HSP's successfully gapped: 751 Length of query: 482 Length of database: 757,237,578 Length adjustment: 130 Effective length of query: 352 Effective length of database: 404,274,708 Effective search space: 142304697216 Effective search space used: 142304697216 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 128 (53.9 bits)