BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0574.0034.p (291 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_00056977.1| COG5297: Cellobiohydrolase A (1,4-beta-cellob... 305 7e-82 ref|ZP_00061908.1| hypothetical protein [Clostridium thermocellu... 303 2e-81 ref|NP_823032.1| putative endo-1,4-beta-glucanase [Streptomyces ... 303 3e-81 emb|CAE51306.1| beta-1,4-xyloglucan hydrolase [Clostridium therm... 302 6e-81 ref|NP_630626.1| putative secreted cellulase [Streptomyces coeli... 289 5e-77 >ref|ZP_00056977.1| COG5297: Cellobiohydrolase A (1,4-beta-cellobiosidase A) [Thermobifida fusca] Length = 925 Score = 305 bits (781), Expect = 7e-82, Method: Compositional matrix adjust. Identities = 152/255 (59%), Positives = 187/255 (73%), Gaps = 7/255 (2%) Query: 39 YNWKSVVTGAGGGFVPGIIFNQSEPDLIYARTDIGGAYRWNQATGSWVSISDAVGWVDWN 98 Y W++V GGGFVPGI+FNQSEPDLIYARTDIGGAYRW+ AT W+ + D VGW DW Sbjct: 52 YTWRNVEI-VGGGFVPGIVFNQSEPDLIYARTDIGGAYRWDPATERWIPLLDHVGWDDWG 110 Query: 99 KNGVDALATDPIDPDKVYMATGTYTNSWD-DNGQIMRSSDRGNTWQSTPLPFKVGGNMPG 157 +GV ++ATDP+DPD+VY A GTYTN WD +NG I RS+DRG TW++T LPFK+GGNMPG Sbjct: 111 HSGVVSIATDPVDPDRVYAAVGTYTNDWDPNNGAIKRSTDRGETWETTELPFKLGGNMPG 170 Query: 158 RSAGERLVIDPNKNSILFFGARSGNGLWKSTDSGVTWSKVSSFPNAGTYIQNPTL--EYG 215 R GERL IDPN NS+L+ GA SG+GLWKSTD G TW KV+SFPN G Y+ +P+ Y Sbjct: 171 RGMGERLAIDPNDNSVLYLGAPSGHGLWKSTDYGKTWQKVTSFPNPGNYVADPSDVGGYL 230 Query: 216 NDLVGLSWITFDQSTGTLGSATQTIYVGVADTASSVYRSTDGGATWTAIPGQPTGXXXPA 275 D G+ W+ FD ++ + G T+ IYVGVAD ++VYRSTDGG TW IPGQPTG Sbjct: 231 GDNQGVVWVVFDPTSSSPGHVTKDIYVGVADKQNTVYRSTDGGQTWERIPGQPTGF---L 287 Query: 276 ASRGAFFYRGSIYYV 290 A +G F + + Y+ Sbjct: 288 AQKGVFDHVNGLLYI 302 >ref|ZP_00061908.1| hypothetical protein [Clostridium thermocellum ATCC 27405] Length = 842 Score = 303 bits (777), Expect = 2e-81, Method: Compositional matrix adjust. Identities = 147/245 (60%), Positives = 187/245 (76%), Gaps = 2/245 (0%) Query: 28 PAIT-RAAASEAYNWKSVVTGAGGGFVPGIIFNQSEPDLIYARTDIGGAYRWNQATGSWV 86 PAI+ +A S Y W +VV G GGGF+PGI+FN++E DLIYAR DIGGAYRW+ +T +W+ Sbjct: 26 PAISSQAVTSVPYKWDNVVIGGGGGFMPGIVFNETEKDLIYARADIGGAYRWDPSTETWI 85 Query: 87 SISDAVGWVDWNKNGVDALATDPIDPDKVYMATGTYTNSWDDN-GQIMRSSDRGNTWQST 145 + D +++ GV+++ATDP+DP++VY+A G YTN W N G I+RS+DRG TW+ T Sbjct: 86 PLLDHFQMDEYSYYGVESIATDPVDPNRVYIAAGMYTNDWLPNMGAILRSTDRGETWEKT 145 Query: 146 PLPFKVGGNMPGRSAGERLVIDPNKNSILFFGARSGNGLWKSTDSGVTWSKVSSFPNAGT 205 LPFK+GGNMPGRS GERL IDPN N IL+ G R GNGLW+STD GVTWSKV SFPN GT Sbjct: 146 ILPFKMGGNMPGRSMGERLAIDPNDNRILYLGTRCGNGLWRSTDYGVTWSKVESFPNPGT 205 Query: 206 YIQNPTLEYGNDLVGLSWITFDQSTGTLGSATQTIYVGVADTASSVYRSTDGGATWTAIP 265 YI +P +Y D++G+ W+ FD+S+ T G+ T+TIYVGVAD S+YRSTDGG TW A+P Sbjct: 206 YIYDPNFDYTKDIIGVVWVVFDKSSSTPGNPTKTIYVGVADKNESIYRSTDGGVTWKAVP 265 Query: 266 GQPTG 270 GQP G Sbjct: 266 GQPKG 270 >ref|NP_823032.1| putative endo-1,4-beta-glucanase [Streptomyces avermitilis MA-4680] dbj|BAC69567.1| putative endo-1,4-beta-glucanase [Streptomyces avermitilis MA-4680] Length = 882 Score = 303 bits (776), Expect = 3e-81, Method: Compositional matrix adjust. Identities = 155/274 (56%), Positives = 200/274 (72%), Gaps = 11/274 (4%) Query: 5 KRIRKLGLVVLLAA-----MTSSLFTAFPAITRAAASEAYNWKSVVTGAGGGFVPGIIFN 59 +R R V+ LAA +L + P T AA + Y+WK+ GGGFVPGI+FN Sbjct: 2 RRTRIFTAVLALAAGLPAGTPPALAASAPTATIAA--DTYSWKNARV-DGGGFVPGIVFN 58 Query: 60 QSEPDLIYARTDIGGAYRWNQATGSWVSISDAVGWVDWNKNGVDALATDPIDPDKVYMAT 119 +SE +L YARTDIGGAYRW +++ +W + D+VGW DW GV +LA+D +DP+KVY A Sbjct: 59 RSEKNLAYARTDIGGAYRWAESSKTWTPLLDSVGWSDWGHTGVVSLASDSVDPNKVYAAV 118 Query: 120 GTYTNSWD-DNGQIMRSSDRGNTWQSTPLPFKVGGNMPGRSAGERLVIDPNKNSILFFGA 178 GTYTNSWD NG ++RS DRG +WQ T LPFK+GGNMPGR GERL +DPN+NS+L+ GA Sbjct: 119 GTYTNSWDPGNGAVLRSGDRGASWQKTDLPFKLGGNMPGRGMGERLAVDPNRNSVLYLGA 178 Query: 179 RSGNGLWKSTDSGVTWSKVSSFPNAGTYIQNPTLE--YGNDLVGLSWITFDQSTGTLGSA 236 SG GLW+STDSG +WS+V+ FPN GTY+Q+ T Y +D G+ W+TFD+STG+ GS+ Sbjct: 179 PSGKGLWRSTDSGASWSQVTDFPNVGTYVQDATDTSGYASDNQGIVWVTFDESTGSPGSS 238 Query: 237 TQTIYVGVADTASSVYRSTDGGATWTAIPGQPTG 270 T+T+YVGVAD +SVYRSTD GATW+ + GQPTG Sbjct: 239 TRTVYVGVADKDNSVYRSTDAGATWSRLAGQPTG 272 >emb|CAE51306.1| beta-1,4-xyloglucan hydrolase [Clostridium thermocellum] Length = 842 Score = 302 bits (773), Expect = 6e-81, Method: Compositional matrix adjust. Identities = 146/245 (59%), Positives = 186/245 (75%), Gaps = 2/245 (0%) Query: 28 PAIT-RAAASEAYNWKSVVTGAGGGFVPGIIFNQSEPDLIYARTDIGGAYRWNQATGSWV 86 PAI+ +A S Y W +VV G GGGF+PGI+FN++E DLIYAR DIGGAYRW+ +T +W+ Sbjct: 26 PAISSQAVTSVPYKWDNVVIGGGGGFMPGIVFNETEKDLIYARADIGGAYRWDPSTETWI 85 Query: 87 SISDAVGWVDWNKNGVDALATDPIDPDKVYMATGTYTNSWDDN-GQIMRSSDRGNTWQST 145 + D +++ GV+++ATDP+DP++VY+ G YTN W N G I+RS+DRG TW+ T Sbjct: 86 PLLDHFQMDEYSYYGVESIATDPVDPNRVYIVAGMYTNDWLPNMGAILRSTDRGETWEKT 145 Query: 146 PLPFKVGGNMPGRSAGERLVIDPNKNSILFFGARSGNGLWKSTDSGVTWSKVSSFPNAGT 205 LPFK+GGNMPGRS GERL IDPN N IL+ G R GNGLW+STD GVTWSKV SFPN GT Sbjct: 146 ILPFKMGGNMPGRSMGERLAIDPNDNRILYLGTRCGNGLWRSTDYGVTWSKVESFPNPGT 205 Query: 206 YIQNPTLEYGNDLVGLSWITFDQSTGTLGSATQTIYVGVADTASSVYRSTDGGATWTAIP 265 YI +P +Y D++G+ W+ FD+S+ T G+ T+TIYVGVAD S+YRSTDGG TW A+P Sbjct: 206 YIYDPNFDYTKDIIGVVWVVFDKSSSTPGNPTKTIYVGVADKNESIYRSTDGGVTWKAVP 265 Query: 266 GQPTG 270 GQP G Sbjct: 266 GQPKG 270 >ref|NP_630626.1| putative secreted cellulase [Streptomyces coelicolor A3(2)] pir||T35237 probable secreted cellulase - Streptomyces coelicolor emb|CAA20642.1| putative secreted cellulase [Streptomyces coelicolor A3(2)] Length = 890 Score = 289 bits (739), Expect = 5e-77, Method: Compositional matrix adjust. Identities = 140/238 (58%), Positives = 181/238 (76%), Gaps = 5/238 (2%) Query: 37 EAYNWKSVVTGAGGGFVPGIIFNQSEPDLIYARTDIGGAYRWNQATGSWVSISDAVGWVD 96 ++Y WK+ GGGFVPGI+FN++E DL YARTDIGGAYRW + + +W + D VGW D Sbjct: 39 DSYTWKNARI-DGGGFVPGIVFNRTEKDLAYARTDIGGAYRWQEESHTWTPLLDHVGWDD 97 Query: 97 WNKNGVDALATDPIDPDKVYMATGTYTNSWD-DNGQIMRSSDRGNTWQSTPLPFKVGGNM 155 W GV ALA+D +DPD+VY A GTYTN WD NG ++RS+DRG +W+ LPFK+GGNM Sbjct: 98 WGHTGVVALASDAVDPDRVYAAVGTYTNDWDPTNGAVLRSADRGASWEKADLPFKLGGNM 157 Query: 156 PGRSAGERLVIDPNKNSILFFGARSGNGLWKSTDSGVTWSKVSSFPNAGTYIQNP--TLE 213 PGR GERL +DP+ N +L+ GA SG+GLW+STD+GVTWS+V++FPN G Y Q+P T Sbjct: 158 PGRGMGERLAVDPHDNDVLYLGAPSGHGLWRSTDAGVTWSEVTAFPNPGNYAQDPNDTSG 217 Query: 214 YGNDLVGLSWITFDQST-GTLGSATQTIYVGVADTASSVYRSTDGGATWTAIPGQPTG 270 Y +D G++W+TFD+ST G G+AT+T+YVGVAD ++VYRSTD GATW + GQPTG Sbjct: 218 YASDNQGITWVTFDESTGGGAGTATRTLYVGVADKENAVYRSTDAGATWERLAGQPTG 275 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.316 0.132 0.413 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 483,937,624 Number of extensions: 22029560 Number of successful extensions: 57681 Number of sequences better than 1.0e-05: 67 Number of HSP's gapped: 57564 Number of HSP's successfully gapped: 77 Length of query: 291 Length of database: 757,237,578 Length adjustment: 124 Effective length of query: 167 Effective length of database: 420,565,302 Effective search space: 70234405434 Effective search space used: 70234405434 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 125 (52.8 bits)