BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0574.0033.p (796 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_00061908.1| hypothetical protein [Clostridium thermocellu... 453 e-126 emb|CAE51306.1| beta-1,4-xyloglucan hydrolase [Clostridium therm... 453 e-126 gb|AAK06388.1| glycosyl hydrolase 5 [Caldicellulosiruptor sp. To... 449 e-124 ref|NP_823032.1| putative endo-1,4-beta-glucanase [Streptomyces ... 449 e-124 ref|ZP_00056977.1| COG5297: Cellobiohydrolase A (1,4-beta-cellob... 447 e-124 >ref|ZP_00061908.1| hypothetical protein [Clostridium thermocellum ATCC 27405] Length = 842 Score = 453 bits (1166), Expect = e-126, Method: Compositional matrix adjust. Identities = 237/449 (52%), Positives = 296/449 (65%), Gaps = 25/449 (5%) Query: 1 VDAQHPDTVMVSSLNAWWPDEVIFRSTNGGATWSRIWDWGFYPERTYKFAMDITAAPWLN 60 VD Q+PD +MV+S+NAWWPDE IFRST+GGATW IW+WG YPER + +DI+AAPWL+ Sbjct: 335 VDRQNPDIIMVTSMNAWWPDEYIFRSTDGGATWKNIWEWGMYPERILHYEIDISAAPWLD 394 Query: 61 HGITNSTSLDPSPKLGWMMGDLEIDPFNSDRMMYGTGATIYRTNNLTSWDSGGKVNIAVM 120 G T + +PKLGWM+GD+EIDPFNSDRMMY TGATIY +NLT WD GGKV I V Sbjct: 395 WG-TEKQLPEINPKLGWMIGDIEIDPFNSDRMMYVTGATIYGCDNLTDWDRGGKVKIEVK 453 Query: 121 AKGVEETAVLGLISPPSGTAHLITALGDVSGFRYEDLTQAPVKFQTSPSWATTTGIDYAE 180 A G+EE AVL L+SPP G A L++A+GD+ GF ++DL P K PS+++ TGIDYAE Sbjct: 454 ATGIEECAVLDLVSPPEG-APLVSAVGDLVGFVHDDLKVGPKKMHV-PSYSSGTGIDYAE 511 Query: 181 LNPAYVVRVGGADKEKTPSMKSIGISNDGGVNWYMPNSEPSNGTKTTAGQGQVAVSASGN 240 L P ++ V AD +K I S DGG NW+ P +E N + G G VAV+A Sbjct: 512 LVPNFMALVAKADLY---DVKKISFSYDGGRNWFQPPNEAPN----SVGGGSVAVAADAK 564 Query: 241 SILWSTSDIGVYYSKTTGNSWNVSSGVPAGAKVASDRVNPNKFYAFYAGTFYIXXXXXXX 300 S++W+ + + GNSW V + + GA VASDRVN KFYAFY G FYI Sbjct: 565 SVIWTPENASPAVTTDNGNSWKVCTNLGMGAVVASDRVNGKKFYAFYNGKFYISTDGGLT 624 Query: 301 XXXXXXXXXXXNNVGGLQPNQAQISMKAMPGIEGDIWFAGGNAVEGKYGLWHSTDSGTSF 360 + Q ++ +KA+PG EG +W A A EG GLW STD G +F Sbjct: 625 F----------TDTKAPQLPKSVNKIKAVPGKEGHVWLA---AREG--GLWRSTDGGYTF 669 Query: 361 TKLTNVEEADLIGFGMAAPGQNYMALYTVAKIDGVRGVFRSDDAGSSWVRINDDAHQYAK 420 KL+NV+ A ++GFG AAPGQ+YMA+Y KID V G FRSDDAG +WVRINDD H Y Sbjct: 670 EKLSNVDTAHVVGFGKAAPGQDYMAIYITGKIDNVLGFFRSDDAGKTWVRINDDEHGYGA 729 Query: 421 INMAITGDPRVYGRVYLGTNGRGTLYADP 449 ++ AITGDPRVYGRVY+ TNGRG +Y +P Sbjct: 730 VDTAITGDPRVYGRVYIATNGRGIVYGEP 758 >emb|CAE51306.1| beta-1,4-xyloglucan hydrolase [Clostridium thermocellum] Length = 842 Score = 453 bits (1166), Expect = e-126, Method: Compositional matrix adjust. Identities = 237/449 (52%), Positives = 296/449 (65%), Gaps = 25/449 (5%) Query: 1 VDAQHPDTVMVSSLNAWWPDEVIFRSTNGGATWSRIWDWGFYPERTYKFAMDITAAPWLN 60 VD Q+PD +MV+S+NAWWPDE IFRST+GGATW IW+WG YPER + +DI+AAPWL+ Sbjct: 335 VDRQNPDIIMVTSMNAWWPDEYIFRSTDGGATWKNIWEWGMYPERILHYEIDISAAPWLD 394 Query: 61 HGITNSTSLDPSPKLGWMMGDLEIDPFNSDRMMYGTGATIYRTNNLTSWDSGGKVNIAVM 120 G T + +PKLGWM+GD+EIDPFNSDRMMY TGATIY +NLT WD GGKV I V Sbjct: 395 WG-TEKQLPEINPKLGWMIGDIEIDPFNSDRMMYVTGATIYGCDNLTDWDRGGKVKIEVK 453 Query: 121 AKGVEETAVLGLISPPSGTAHLITALGDVSGFRYEDLTQAPVKFQTSPSWATTTGIDYAE 180 A G+EE AVL L+SPP G A L++A+GD+ GF ++DL P K PS+++ TGIDYAE Sbjct: 454 ATGIEECAVLDLVSPPEG-APLVSAVGDLVGFVHDDLKVGPKKMHV-PSYSSGTGIDYAE 511 Query: 181 LNPAYVVRVGGADKEKTPSMKSIGISNDGGVNWYMPNSEPSNGTKTTAGQGQVAVSASGN 240 L P ++ V AD +K I S DGG NW+ P +E N + G G VAV+A Sbjct: 512 LVPNFMALVAKADLY---DVKKISFSYDGGRNWFQPPNEAPN----SVGGGSVAVAADAK 564 Query: 241 SILWSTSDIGVYYSKTTGNSWNVSSGVPAGAKVASDRVNPNKFYAFYAGTFYIXXXXXXX 300 S++W+ + + GNSW V + + GA VASDRVN KFYAFY G FYI Sbjct: 565 SVIWTPENASPAVTTDNGNSWKVCTNLGMGAVVASDRVNGKKFYAFYNGKFYISTDGGLT 624 Query: 301 XXXXXXXXXXXNNVGGLQPNQAQISMKAMPGIEGDIWFAGGNAVEGKYGLWHSTDSGTSF 360 + Q ++ +KA+PG EG +W A A EG GLW STD G +F Sbjct: 625 F----------TDTKAPQLPKSVNKIKAVPGKEGHVWLA---AREG--GLWRSTDGGYTF 669 Query: 361 TKLTNVEEADLIGFGMAAPGQNYMALYTVAKIDGVRGVFRSDDAGSSWVRINDDAHQYAK 420 KL+NV+ A ++GFG AAPGQ+YMA+Y KID V G FRSDDAG +WVRINDD H Y Sbjct: 670 EKLSNVDTAHVVGFGKAAPGQDYMAIYITGKIDNVLGFFRSDDAGKTWVRINDDEHGYGA 729 Query: 421 INMAITGDPRVYGRVYLGTNGRGTLYADP 449 ++ AITGDPRVYGRVY+ TNGRG +Y +P Sbjct: 730 VDTAITGDPRVYGRVYIATNGRGIVYGEP 758 >gb|AAK06388.1| glycosyl hydrolase 5 [Caldicellulosiruptor sp. Tok7B.1] Length = 996 Score = 449 bits (1154), Expect = e-124, Method: Compositional matrix adjust. Identities = 227/448 (50%), Positives = 290/448 (64%), Gaps = 22/448 (4%) Query: 1 VDAQHPDTVMVSSLNAWWPDEVIFRSTNGGATWSRIWDWGFYPERTYKFAMDITAAPWLN 60 VDAQ+P VMV++L++WWPD I+RS +GG TW IW+W YP RT + MDI+AAPWLN Sbjct: 334 VDAQNPKVVMVAALSSWWPDTYIWRSIDGGETWKCIWEWNGYPNRTLHYNMDISAAPWLN 393 Query: 61 HGITNSTSLDPSPKLGWMMGDLEIDPFNSDRMMYGTGATIYRTNNLTSWDSGGKVNIAVM 120 G T+ T + SPKLGWM+G LEIDPFNSD M+YGTGA +Y ++LT+WD G + I V Sbjct: 394 FGXTBPTPPEVSPKLGWMVGTLEIDPFNSDXMLYGTGAXLYGCDDLTNWDKGQNITIKVK 453 Query: 121 AKGVEETAVLGLISPPSGTAHLITALGDVSGFRYEDLTQAPVKFQTSPSWATTTGIDYAE 180 A G+EET+V LISPP G HL +ALGD++GFR+EDL +AP P+ TTT ID+AE Sbjct: 454 AIGIEETSVQALISPPVG-PHLFSALGDIAGFRHEDLEKAPNWTYVQPNMGTTTDIDFAE 512 Query: 181 LNPAYVVRVGGADKEKTPSMKSIGISNDGGVNWYMPNSEPSNGTKTTAGQGQVAVSASGN 240 LNP ++VRVG DK+ P+ IG S DGG +W+ N+EP + T+ G VA +A G+ Sbjct: 513 LNPNFMVRVGNVDKQWNPNTNRIGFSYDGGKSWFQGNTEP----QGTSEGGTVAAAADGS 568 Query: 241 SILWSTSDIGVYYSKTTGNSWNVSSGVPAGAKVASDRVNPNKFYAFYAGTFYIXXXXXXX 300 +++W+ V YS GN W + VP+ A V SDRVNPNKFYAF G FYI Sbjct: 569 AVVWAPKGAKVCYSTDNGNKWVECANVPSEAIVYSDRVNPNKFYAFKNGKFYISADKGKT 628 Query: 301 XXXXXXXXXXXNNVGGLQPNQAQISMKAMPGIEGDIWFAGGNAVEGKYGLWHSTDSGTSF 360 + + K +PGIEGDIW G N G+WHSTD G SF Sbjct: 629 FIESPAAGLPISG-----------NFKTVPGIEGDIWLVGNN------GMWHSTDGGYSF 671 Query: 361 TKLTNVEEADLIGFGMAAPGQNYMALYTVAKIDGVRGVFRSDDAGSSWVRINDDAHQYAK 420 K++ VE+A IGFG A G+ Y A+YT AKI+GVRG+FRSDD +W+RINDD HQ+ Sbjct: 672 VKISGVEDAASIGFGKPAEGETYPAIYTYAKINGVRGIFRSDDCDKTWIRINDDKHQFGC 731 Query: 421 INMAITGDPRVYGRVYLGTNGRGTLYAD 448 N ITGDPRVYGRV++ TNG G + + Sbjct: 732 ANADITGDPRVYGRVFVATNGLGIKWGE 759 Score = 144 bits (363), Expect = 9e-33, Method: Compositional matrix adjust. Identities = 71/150 (47%), Positives = 102/150 (68%), Gaps = 1/150 (0%) Query: 647 ALKVQMYNSGTAASANTISPKFKLVNTGTSAITLSGVKLRYYYTIDGEQTQNFFCDWSQV 706 LKV N+ T+ASA +I P FK+VN G+S++ LS VK+RY+YT+DG++ Q+ CDW+Q+ Sbjct: 844 GLKVLYKNNETSASAGSIRPWFKIVNGGSSSVDLSRVKIRYWYTVDGDKPQSAVCDWAQI 903 Query: 707 GSANVTGSFVKMPSATTGADYYLELGFNXXXXXXXXXXXI-DIQARVAKSDWTNYTQTGD 765 G++NVT +FVK+ S +GADYYLE+GF+ DIQ R K+DW+NY Q D Sbjct: 904 GASNVTFNFVKLSSGVSGADYYLEVGFSSGAGQLQPGKDTGDIQVRFNKNDWSNYNQADD 963 Query: 766 YSFNGADTNYADWSKAPAYISGTLVWGNEP 795 +S+ + T+Y + +K Y+ G LVWG EP Sbjct: 964 WSWLQSMTDYGENAKVTLYVDGVLVWGQEP 993 >ref|NP_823032.1| putative endo-1,4-beta-glucanase [Streptomyces avermitilis MA-4680] dbj|BAC69567.1| putative endo-1,4-beta-glucanase [Streptomyces avermitilis MA-4680] Length = 882 Score = 449 bits (1154), Expect = e-124, Method: Compositional matrix adjust. Identities = 230/448 (51%), Positives = 294/448 (65%), Gaps = 21/448 (4%) Query: 1 VDAQHPDTVMVSSLNAWWPDEVIFRSTNGGATWSRIWDWGFYPERTYKFAMDITAAPWLN 60 VD QHP TVM ++ ++WWPD +FRST+ G TW++ WD+ YP R+ +F MD++++PWL Sbjct: 335 VDRQHPGTVMATAYSSWWPDTQLFRSTDSGGTWTKAWDYTSYPSRSNRFTMDVSSSPWLT 394 Query: 61 HGITNSTSLDPSPKLGWMMGDLEIDPFNSDRMMYGTGATIYRTNNLTSWDSGGKVNIAVM 120 G N + +PKLGWM LEIDPF+S RMMYGTGAT+Y T+NLT+WDSG + I M Sbjct: 395 WGA-NPAPPEQTPKLGWMTESLEIDPFDSARMMYGTGATVYGTDNLTNWDSGSQFTIKPM 453 Query: 121 AKGVEETAVLGLISPPSGTAHLITALGDVSGFRYEDLTQAPVKFQTSPSWATTTGIDYAE 180 A+G+EETAV L SPPSG A L +ALGD+ GFR+ DLT P TSP++ T+T +DYAE Sbjct: 454 ARGLEETAVNDLASPPSGGAQLFSALGDIGGFRHTDLTTVPSLMYTSPNFTTSTSLDYAE 513 Query: 181 LNPAYVVRVGGADKEKTPSMKSIGISNDGGVNWYMPNSEPSNGTKTTAGQGQVAVSASGN 240 +P VVRVG D S + S D G NW+ ++PS +G G VA ++ G+ Sbjct: 514 TDPGTVVRVGNLD-----SGPHVAFSTDNGANWFA-GADPSG----VSGGGTVAAASDGS 563 Query: 241 SILWSTSDIGVYYSKTTGNSWNVSSGVPAGAKVASDRVNPNKFYAFYAGTFYIXXXXXXX 300 +WS + GV Y+ G SW+ S+G+PAGA V SDRV+P FY F +G FY+ Sbjct: 564 RFVWSPAGTGVQYTTGFGTSWSASAGLPAGAIVESDRVDPKTFYGFKSGRFYVSSDGGAT 623 Query: 301 XXXXXXXXXXXNNVGGLQPNQAQISMKAMPGIEGDIWFAGGNAVEGKYGLWHSTDSGTSF 360 GL P+ + KA+PG +GDIW AGG A +G YGLWHSTD G +F Sbjct: 624 FTASAAT--------GL-PSGDSVRFKALPGTKGDIWLAGG-ASDGAYGLWHSTDGGAAF 673 Query: 361 TKLTNVEEADLIGFGMAAPGQNYMALYTVAKIDGVRGVFRSDDAGSSWVRINDDAHQYAK 420 TKL V++AD IGFG AA G +Y LYT AKI GVRG+FRS D G+SW R+NDDAHQ+ Sbjct: 674 TKLATVDQADTIGFGKAATGASYQTLYTSAKIGGVRGIFRSTDKGASWTRVNDDAHQWGW 733 Query: 421 INMAITGDPRVYGRVYLGTNGRGTLYAD 448 AITGDPRVYGRVY+ TNGRG +Y D Sbjct: 734 TGAAITGDPRVYGRVYVSTNGRGIVYGD 761 >ref|ZP_00056977.1| COG5297: Cellobiohydrolase A (1,4-beta-cellobiosidase A) [Thermobifida fusca] Length = 925 Score = 447 bits (1149), Expect = e-124, Method: Compositional matrix adjust. Identities = 237/448 (52%), Positives = 303/448 (67%), Gaps = 19/448 (4%) Query: 1 VDAQHPDTVMVSSLNAWWPDEVIFRSTNGGATWSRIWDWGFYPERTYKFAMDITAAPWLN 60 +D Q+PDT+MV S WWPD I+RST+ G TWSRIW++ YP+RT ++ DI+AAPWL+ Sbjct: 348 IDRQNPDTIMVVSQILWWPDIQIWRSTDRGETWSRIWEFSGYPDRTLRYNHDISAAPWLD 407 Query: 61 HGITNSTSLDPSPKLGWMMGDLEIDPFNSDRMMYGTGATIYRTNNLTSWDSGGKVNIAVM 120 ++ + SPKLGWM EIDPFNSDRM+YGTGATIY ++NLT+WD G K++I V Sbjct: 408 FNRQDNPP-EVSPKLGWMTQAFEIDPFNSDRMLYGTGATIYGSDNLTNWDEGKKIDIKVR 466 Query: 121 AKGVEETAVLGLISPPSGTAHLITALGDVSGFRYEDLTQAPVKFQTSPSWATTTGIDYAE 180 A+G+EETAV LI+PP G L++ALGD+ GF ++D+T P SP T ID+AE Sbjct: 467 AQGIEETAVQDLIAPP-GDTELVSALGDIGGFVHDDITVVPDAMFDSPFHGNTRSIDFAE 525 Query: 181 LNPAYVVRVGGADKEKTPSMKSIGISNDGGVNWYMPNSEPSNGTKTTAGQGQVAVSASGN 240 LNP+ + RVG A + S IGIS GG +W+ EPS T G G VAV+A G+ Sbjct: 526 LNPSVMARVGEAVDGEVDSH--IGISTSGGSHWWA-GQEPSGVT----GAGTVAVNADGS 578 Query: 241 SILWSTSDIGVYYSKTTGNSWNVSSGVPAGAKVASDRVNPNKFYAFYAGTFYIXXXXXXX 300 I+WS GV+YS T G+SW S GVPAGA+V +DRVNP+KFYAF GTFY Sbjct: 579 RIVWSPDGTGVHYSTTLGSSWTPSQGVPAGARVEADRVNPDKFYAFANGTFY-------- 630 Query: 301 XXXXXXXXXXXNNVGGLQPNQAQISMKAMPGIEGDIWFAGGNAVEGKYGLWHSTDSGTSF 360 ++ GL P + I A+PG EGDIW AGG YG+W STDSG +F Sbjct: 631 TSTDGGATFTKSSAAGL-PTKGNIRFAAVPGHEGDIWLAGGE-TNSTYGMWRSTDSGATF 688 Query: 361 TKLTNVEEADLIGFGMAAPGQNYMALYTVAKIDGVRGVFRSDDAGSSWVRINDDAHQYAK 420 T++T V+E D++GFG APG++Y A+YT +KI+GVRG+FRSDDAG++WVRINDD HQ+A Sbjct: 689 TRITAVDEGDVVGFGKPAPGRSYPAVYTSSKINGVRGIFRSDDAGTTWVRINDDQHQWAW 748 Query: 421 INMAITGDPRVYGRVYLGTNGRGTLYAD 448 AITGDP VYGRVY+GTNGRG + D Sbjct: 749 TGAAITGDPDVYGRVYIGTNGRGVIVGD 776 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.313 0.129 0.385 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 1,163,517,633 Number of extensions: 48412607 Number of successful extensions: 96925 Number of sequences better than 1.0e-05: 104 Number of HSP's gapped: 96665 Number of HSP's successfully gapped: 137 Length of query: 796 Length of database: 757,237,578 Length adjustment: 134 Effective length of query: 662 Effective length of database: 393,414,312 Effective search space: 260440274544 Effective search space used: 260440274544 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 130 (54.7 bits)