BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0574.0029.p (230 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_617802.1| conserved hypothetical protein [Methanosarcina ... 162 6e-39 ref|NP_301339.1| conserved hypothetical protein [Mycobacterium l... 140 3e-32 ref|ZP_00109859.1| COG0500: SAM-dependent methyltransferases [No... 134 1e-30 ref|NP_247008.1| conserved hypothetical protein [Methanococcus j... 132 6e-30 ref|NP_276203.1| conserved protein [Methanothermobacter thermaut... 127 1e-28 >ref|NP_617802.1| conserved hypothetical protein [Methanosarcina acetivorans str. C2A] gb|AAM06282.1| conserved hypothetical protein [Methanosarcina acetivorans str. C2A] Length = 261 Score = 162 bits (410), Expect = 6e-39, Method: Compositional matrix adjust. Identities = 85/215 (39%), Positives = 125/215 (58%), Gaps = 4/215 (1%) Query: 15 GRTFEEYLRMFNLQPGELKGRSILDCPAGACSFTAEANKLGAASVAADIAYYYSAEALRD 74 GR+F+EY+RMF+L P +LK R IL C G SF E + G ++ D YY+SA+ + Sbjct: 48 GRSFDEYVRMFSLTPADLK-RKILGCGDGPASFNVELTERGGNIISVDPIYYFSADRIMQ 106 Query: 75 KGLKDIEHTVQQLDKVQDNFVWKEFTSIHXXXXXXXXXXXXXXXD--RQQNSSRYIPVEL 132 K + + + Q K +D FVW+E S+ D + RYIP EL Sbjct: 107 KISETYDDIIDQTRKNRDQFVWQEIGSVEELGRVRMSAMEKFLKDFPKGMMQKRYIPGEL 166 Query: 133 PLLPFADRSFDLTLSAHFLFMYGDRLDYDFHLRTLQELMRVTKSEIRIFPLVDLSTRRYH 192 P LPF ++ FDL L +HFLF+Y D L +FHLR L+EL RV+ +E+RIFPL+D++ R Sbjct: 167 PSLPFGNKEFDLALCSHFLFLYTDNLSLEFHLRALEELCRVS-NEVRIFPLLDVNASRST 225 Query: 193 KLDQLVCAAQVQGWTAEEQQVPYEFQKGADSMLRL 227 +D ++ + + EE +V YEFQ+G ++ML + Sbjct: 226 YVDTVIDFLREEKRDVEEVKVAYEFQRGGNTMLNI 260 >ref|NP_301339.1| conserved hypothetical protein [Mycobacterium leprae] pir||T44731 hypothetical protein [imported] - Mycobacterium leprae emb|CAA22698.1| hypothetical protein [Mycobacterium leprae] emb|CAC29816.1| conserved hypothetical protein [Mycobacterium leprae] Length = 234 Score = 140 bits (352), Expect = 3e-32, Method: Compositional matrix adjust. Identities = 87/231 (37%), Positives = 116/231 (50%), Gaps = 16/231 (6%) Query: 8 LERIVFIGRTFEEYLRMFNLQPGELKGRSILDCPAGACSFTAEANKLGAASVAADIAYYY 67 + ++V R F+EY MF L +L +ILDCP GA S TAE N G S+A D Y Sbjct: 7 IGKVVITSRGFDEYRAMFALSAMDLNA-TILDCPGGASSATAEINAAGGRSMAVDPFYG- 64 Query: 68 SAEALRDKGLKDIEHTVQ-QLDKVQ-------DNFVWKEFTSIHXXXXXXXXXXXXXXXD 119 R+ + D+ +Q + D+ D + W F+S D Sbjct: 65 -----RNLSVADLASFIQAETDRGNAYVRAHPDEYRWTFFSSPEHHHQSRSRAGRLFLHD 119 Query: 120 RQQNSSRYIPVELPLLPFADRSFDLTLSAHFLFMYGDRLDYDFHLRTLQELMRVTKSEIR 179 N S Y+ LP LPF+ +FDL LS+H LF Y DRL DFH + + ELMRVT+ E+R Sbjct: 120 YCANPSNYVEAALPRLPFSTHAFDLVLSSHLLFTYADRLSPDFHYQAINELMRVTRIELR 179 Query: 180 IFPLVDLSTRRYHKLDQLVCAAQVQGWTAEEQQVPYEFQKGADSMLRLLRV 230 IFPLV + + RY LD L+ + G E V YEFQ G + ML R+ Sbjct: 180 IFPLVVMGSIRY-DLDDLLARLRADGVHGEVVAVDYEFQAGGNQMLVCSRL 229 >ref|ZP_00109859.1| COG0500: SAM-dependent methyltransferases [Nostoc punctiforme] Length = 234 Score = 134 bits (338), Expect = 1e-30, Method: Compositional matrix adjust. Identities = 71/224 (31%), Positives = 121/224 (54%), Gaps = 4/224 (1%) Query: 8 LERIVFIGRTFEEYLRMFNLQPGELKGRSILDCPAGACSFTAEANKLGAASVAADIAYYY 67 L+++V GR+ +EY+++FNL +L + I+ G SF AE + G + ++ D Y + Sbjct: 5 LDQVVPFGRSMDEYIKIFNLTDADL-NKKIIGIGDGPASFNAEMTRQGKSVISVDPLYQF 63 Query: 68 SAEALRDKGLKDIEHTVQQLDKVQDNFVWKEFTSIHXXXXXXXXXXXXXXXDRQ--QNSS 125 S + + + + +++ + Q+ +N+VW S D + + S+ Sbjct: 64 SGDEILQRFNEVLDNIINQVKATSNNWVWSYHKSPDDLRHNRVKVIREFLSDYENGKKSN 123 Query: 126 RYIPVELPLLPFADRSFDLTLSAHFLFMYGDRLDYDFHLRTLQELMRVTKSEIRIFPLVD 185 RYI E P L F + F++ L +H LF+Y D LDYDFHL ++ E++R+ K EIRIFPL+ Sbjct: 124 RYIVDEFPKLKFKTQEFEIALCSHLLFLYSDHLDYDFHLNSIGEMLRIAK-EIRIFPLIT 182 Query: 186 LSTRRYHKLDQLVCAAQVQGWTAEEQQVPYEFQKGADSMLRLLR 229 L + LD++V G+ + + V YE Q G + ML++ R Sbjct: 183 LMWKHSQHLDEIVKHYTSIGYKIDIENVEYELQPGGNKMLKITR 226 >ref|NP_247008.1| conserved hypothetical protein [Methanococcus jannaschii] sp|Q60353|Y045_METJA Hypothetical protein MJ0045 pir||E64305 conserved hypothetical protein MJ0045 - Methanococcus jannaschii gb|AAB98025.1| conserved hypothetical protein [Methanococcus jannaschii] Length = 221 Score = 132 bits (332), Expect = 6e-30, Method: Compositional matrix adjust. Identities = 76/225 (33%), Positives = 119/225 (52%), Gaps = 8/225 (3%) Query: 6 LDLERIVFIGRTFEEYLRMFNLQPGELKGRSILDCPAGACSFTAEANKLGAASVAADIAY 65 L+L+ V +GR F EY+RMFNL +L ILD +G SF AE NK G ++D Y Sbjct: 4 LNLKDTVLLGRNFNEYVRMFNLNE-DLLSNKILDVASGVSSFCAEGNKKGYNITSSDKIY 62 Query: 66 YYSAEALRDKGLKDIEHTVQQLDKV-QDNFVWKEFTSIHXXXXXXXXXXXXXXXDRQQNS 124 E + +K KD++ + L + ++NF W EF ++ D + N Sbjct: 63 NLKPEEIEEKCKKDLDFMEKHLRGMFKNNFNWNEFKTVDEWKKTRERTYKTFIEDYKTNR 122 Query: 125 SRYIPVELPLLPFADRSFDLTLSAHFLFMYGDRLDYDFHLRTLQELMRVTKSEIRIFPLV 184 RYI P F D F ++L HFL +Y + L+Y FH T+ EL+R+++ EIRIFP++ Sbjct: 123 KRYIYTTYPKTNFKDDEFAISLVGHFLLLYDNILNYQFHKETIDELLRISE-EIRIFPIL 181 Query: 185 DLSTRRYHKLDQLVCAAQVQGWTAEEQQVPYEFQKGADSMLRLLR 229 +L + LD++ ++ + A ++ YEF KG + +L + R Sbjct: 182 NLRGEKSIFLDKI-----LKEYKARIEKTDYEFMKGGNKVLIIRR 221 >ref|NP_276203.1| conserved protein [Methanothermobacter thermautotrophicus] pir||G69009 conserved hypothetical protein MTH1075 - Methanobacterium thermoautotrophicum (strain Delta H) gb|AAB85564.1| conserved protein [Methanothermobacter thermautotrophicus str. Delta H] Length = 222 Score = 127 bits (320), Expect = 1e-28, Method: Compositional matrix adjust. Identities = 77/231 (33%), Positives = 120/231 (51%), Gaps = 32/231 (13%) Query: 8 LERIVFIGRTFEEYLRMFNLQPGELKGRSILDCPAGACSFTAEANKLGAASVAADIAYYY 67 ++ +VFIGR EY+ MF+L P EL+G ILDC AG SF +E +++G + A D Y Sbjct: 8 MKSVVFIGRGLAEYIDMFSLDPMELEGSRILDCAAGVSSFRSEMDEMGFSVTAVDPLYSR 67 Query: 68 SAEALRDKGLKDI-EHTVQQLDKVQD------------NFVWKEFTSIHXXXXXXXXXXX 114 + + ++ + HT+ D ++D V++ F Sbjct: 68 TPDEIKKLACESFRSHTMNHRDFLRDFRVDGIPVEDYRRMVFRRFLD------------- 114 Query: 115 XXXXDRQQNSSRYIPVELPLLPFADRSFDLTLSAHFLFMYGDRLDYDFHLRTLQELMRVT 174 D ++N S YI ELP LPF D FDL +SA+FLF+Y D LDY FH+ +++E++R+ Sbjct: 115 ----DYKRNPSEYIAGELPFLPFDDGEFDLVVSANFLFLYEDLLDYSFHVESVREMLRIG 170 Query: 175 KSEIRIFPLVDLSTRRYHKLDQLVCAAQVQGWTAEEQQVPYEFQKGADSML 225 K E+RIFP+ ++ RR L + + E ++V Y + G + ML Sbjct: 171 K-EVRIFPVYNIH-RRKRSLHLKPLMDEFNDYDIEVRKVKYHEEAGCNEML 219 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.324 0.138 0.406 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 285,984,266 Number of extensions: 9695915 Number of successful extensions: 30657 Number of sequences better than 1.0e-05: 10 Number of HSP's gapped: 30634 Number of HSP's successfully gapped: 10 Length of query: 230 Length of database: 757,237,578 Length adjustment: 121 Effective length of query: 109 Effective length of database: 428,710,599 Effective search space: 46729455291 Effective search space used: 46729455291 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.5 bits) S2: 124 (52.4 bits)