BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0574.0027.p (207 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value dbj|BAA19382.1| FUNCTION UNKNOWN, SIMILAR PRODUCT IN SYNECHOCYST... 249 2e-65 ref|NP_388429.1| similar to hypothetical proteins [Bacillus subt... 249 2e-65 ref|NP_834052.1| NAD(P)H nitroreductase [Bacillus cereus ATCC 14... 239 3e-62 ref|NP_658373.1| Nitroreductase, Nitroreductase family [Bacillus... 239 4e-62 ref|NP_347486.1| Nitroreductase family protein [Clostridium acet... 226 2e-58 >dbj|BAA19382.1| FUNCTION UNKNOWN, SIMILAR PRODUCT IN SYNECHOCYSTIS. [Bacillus subtilis] Length = 237 Score = 249 bits (637), Expect = 2e-65, Method: Compositional matrix adjust. Identities = 119/205 (58%), Positives = 152/205 (74%), Gaps = 1/205 (0%) Query: 3 MSTFSELVQARRSANNFVEGIKIPQSELEEMFSLTRLAPSAYNLQHTHYKVVSDEDLKEK 62 M+ F+ LV RRSA+NF+ G I + +L EMF L LAPSA+NLQHT Y V D+D+KEK Sbjct: 32 MAEFTHLVNERRSASNFLSGHPITKEDLNEMFELVALAPSAFNLQHTKYVTVLDQDVKEK 91 Query: 63 IREAAYGQYKIHTASAVIVVLGDKNAYLQASEIYSGLKMLGAMSEDAFDQTIADIHGAYS 122 +++AA GQYK+ ++SAV++VLGDK AY QA++IY GLK+LG +++ +D + D Y Sbjct: 92 LKQAANGQYKVVSSSAVLLVLGDKQAYQQAADIYEGLKVLGILNKQEYDHMVQDTVSFYE 151 Query: 123 GN-DAFQHDEAIRNASLSAMQFMLIAKDKGWDTCPMIGFDVQAVKAALNLSDNMVPVMLI 181 + F+ DEAIRNASLSAM FML AK+KGWDTCPMIGFD +AVK LN+ D VM+I Sbjct: 152 NRGEQFKRDEAIRNASLSAMMFMLSAKEKGWDTCPMIGFDAEAVKRILNIDDQFEVVMMI 211 Query: 182 TIGKDNKHKIRPRGYRKPVNEFVEF 206 TIGK+ RPRGYRKPVNEFVE+ Sbjct: 212 TIGKEKTESRRPRGYRKPVNEFVEY 236 >ref|NP_388429.1| similar to hypothetical proteins [Bacillus subtilis] sp|P96692|YDFN_BACSU Putative NAD(P)H nitroreductase ydfN pir||D69781 conserved hypothetical protein ydfN - Bacillus subtilis emb|CAB12355.1| ydfN [Bacillus subtilis subsp. subtilis str. 168] Length = 206 Score = 249 bits (637), Expect = 2e-65, Method: Compositional matrix adjust. Identities = 119/205 (58%), Positives = 152/205 (74%), Gaps = 1/205 (0%) Query: 3 MSTFSELVQARRSANNFVEGIKIPQSELEEMFSLTRLAPSAYNLQHTHYKVVSDEDLKEK 62 M+ F+ LV RRSA+NF+ G I + +L EMF L LAPSA+NLQHT Y V D+D+KEK Sbjct: 1 MAEFTHLVNERRSASNFLSGHPITKEDLNEMFELVALAPSAFNLQHTKYVTVLDQDVKEK 60 Query: 63 IREAAYGQYKIHTASAVIVVLGDKNAYLQASEIYSGLKMLGAMSEDAFDQTIADIHGAYS 122 +++AA GQYK+ ++SAV++VLGDK AY QA++IY GLK+LG +++ +D + D Y Sbjct: 61 LKQAANGQYKVVSSSAVLLVLGDKQAYQQAADIYEGLKVLGILNKQEYDHMVQDTVSFYE 120 Query: 123 GN-DAFQHDEAIRNASLSAMQFMLIAKDKGWDTCPMIGFDVQAVKAALNLSDNMVPVMLI 181 + F+ DEAIRNASLSAM FML AK+KGWDTCPMIGFD +AVK LN+ D VM+I Sbjct: 121 NRGEQFKRDEAIRNASLSAMMFMLSAKEKGWDTCPMIGFDAEAVKRILNIDDQFEVVMMI 180 Query: 182 TIGKDNKHKIRPRGYRKPVNEFVEF 206 TIGK+ RPRGYRKPVNEFVE+ Sbjct: 181 TIGKEKTESRRPRGYRKPVNEFVEY 205 >ref|NP_834052.1| NAD(P)H nitroreductase [Bacillus cereus ATCC 14579] gb|AAG33873.1| nitroreductase NAD [Lactobacillus delbrueckii subsp. bulgaricus] gb|AAP11253.1| NAD(P)H nitroreductase [Bacillus cereus ATCC 14579] Length = 208 Score = 239 bits (610), Expect = 3e-62, Method: Compositional matrix adjust. Identities = 110/205 (53%), Positives = 149/205 (72%), Gaps = 1/205 (0%) Query: 3 MSTFSELVQARRSANNFVEGIKIPQSELEEMFSLTRLAPSAYNLQHTHYKVVSDEDLKEK 62 M F EL ARRSA F++GI+IP + +++F LT+L+PS YNLQHTHY V+ D++ KE+ Sbjct: 1 MVNFEELAIARRSAMKFIQGIEIPDEDFKKIFELTKLSPSCYNLQHTHYVVIRDQERKER 60 Query: 63 IREAAYGQYKIHTASAVIVVLGDKNAYLQASEIYSGLKMLGAMSEDAFDQTIADIHGAYS 122 ++E A+GQYK+ +ASAV+ V GDK AY IY G+K+L + E ++ TI G Y Sbjct: 61 LKELAFGQYKVSSASAVVFVCGDKKAYQNVENIYFGMKVLKMIDECEYEDTIRKTKGLYE 120 Query: 123 GN-DAFQHDEAIRNASLSAMQFMLIAKDKGWDTCPMIGFDVQAVKAALNLSDNMVPVMLI 181 G + F DEAIRNASLSAM FM AK G+DTCPMIGFD +AV++ LN+ +++VPV++I Sbjct: 121 GKGERFMEDEAIRNASLSAMTFMYAAKYYGYDTCPMIGFDEKAVQSELNMPEHIVPVLMI 180 Query: 182 TIGKDNKHKIRPRGYRKPVNEFVEF 206 TIGK + K RPRGYRKP++EFV + Sbjct: 181 TIGKSDTTKTRPRGYRKPISEFVTY 205 >ref|NP_658373.1| Nitroreductase, Nitroreductase family [Bacillus anthracis A2012] ref|NP_846793.1| nitroreductase family protein [Bacillus anthracis str. Ames] gb|AAP28279.1| nitroreductase family protein [Bacillus anthracis str. Ames] Length = 207 Score = 239 bits (609), Expect = 4e-62, Method: Compositional matrix adjust. Identities = 110/202 (54%), Positives = 149/202 (73%), Gaps = 1/202 (0%) Query: 6 FSELVQARRSANNFVEGIKIPQSELEEMFSLTRLAPSAYNLQHTHYKVVSDEDLKEKIRE 65 F EL ARRSA FV+GI+IP + +++F LT+L+PS YNLQHTHY V+ D++ KE+++E Sbjct: 3 FEELAIARRSAMKFVQGIEIPDEDFKKIFELTKLSPSCYNLQHTHYVVIRDQERKERLKE 62 Query: 66 AAYGQYKIHTASAVIVVLGDKNAYLQASEIYSGLKMLGAMSEDAFDQTIADIHGAYSGN- 124 A+GQYK+ +ASAVI+V GDK AY IY G+K+L + E ++ TI G Y G Sbjct: 63 LAFGQYKVSSASAVILVCGDKRAYQNVENIYFGMKVLKMIDECEYEDTIKQTKGLYEGKG 122 Query: 125 DAFQHDEAIRNASLSAMQFMLIAKDKGWDTCPMIGFDVQAVKAALNLSDNMVPVMLITIG 184 + F +EAIRNASLSAM FM AK G+DTCPMIGFD +AV++ LN+ +++VPV++ITIG Sbjct: 123 ERFMEEEAIRNASLSAMTFMYAAKYYGYDTCPMIGFDEKAVQSELNMPEHVVPVLMITIG 182 Query: 185 KDNKHKIRPRGYRKPVNEFVEF 206 K + K RPRGYRKP++EFV + Sbjct: 183 KSDTTKTRPRGYRKPISEFVTY 204 >ref|NP_347486.1| Nitroreductase family protein [Clostridium acetobutylicum] pir||G97004 nitroreductase family protein [imported] - Clostridium acetobutylicum gb|AAK78826.1| Nitroreductase family protein [Clostridium acetobutylicum] Length = 208 Score = 226 bits (576), Expect = 2e-58, Method: Compositional matrix adjust. Identities = 106/205 (51%), Positives = 151/205 (73%), Gaps = 1/205 (0%) Query: 3 MSTFSELVQARRSANNFVEGIKIPQSELEEMFSLTRLAPSAYNLQHTHYKVVSDEDLKEK 62 M F +V+ RRSANNF++GI+IP+++ E+F L +LAPS +N+QH +Y VV+D++ KE+ Sbjct: 1 MMDFKRIVEERRSANNFLDGIEIPKNDFIEIFDLLKLAPSCFNIQHANYIVVTDKNKKEE 60 Query: 63 IREAAYGQYKIHTASAVIVVLGDKNAYLQASEIYSGLKMLGAMSEDAFDQTIADIHGAYS 122 +R AA+ QYK+HTASA I+VLGDK AY A ++YSG+ L +S+ ++ + DI Y Sbjct: 61 LRTAAFNQYKLHTASAAILVLGDKEAYKDADKLYSGMVDLKMISKFDYENILNDIKNLYE 120 Query: 123 GN-DAFQHDEAIRNASLSAMQFMLIAKDKGWDTCPMIGFDVQAVKAALNLSDNMVPVMLI 181 G ++F+HDEAIRNAS+SAM FML AK KGWDTCPMIGFD + ++ N+ + +LI Sbjct: 121 GRGESFKHDEAIRNASISAMMFMLAAKYKGWDTCPMIGFDNEKMRKLFNIPERYEIAILI 180 Query: 182 TIGKDNKHKIRPRGYRKPVNEFVEF 206 ++GK++ R RGYRKPV+EFV+F Sbjct: 181 SMGKEDTKSRRLRGYRKPVDEFVKF 205 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.319 0.132 0.373 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 280,822,259 Number of extensions: 10434467 Number of successful extensions: 25753 Number of sequences better than 1.0e-05: 147 Number of HSP's gapped: 25705 Number of HSP's successfully gapped: 147 Length of query: 207 Length of database: 757,237,578 Length adjustment: 120 Effective length of query: 87 Effective length of database: 431,425,698 Effective search space: 37534035726 Effective search space used: 37534035726 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 123 (52.0 bits)