BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0574.0017.p (194 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gb|AAN18034.1| DarS [Pseudomonas aurantiaca] 101 9e-21 dbj|BAC41251.1| isonitrile hydratase [Pseudomonas putida] 98 9e-20 ref|ZP_00085238.1| COG0693: Putative intracellular protease/amid... 98 1e-19 ref|NP_770920.1| bll4280 [Bradyrhizobium japonicum] >gi|27352542... 97 2e-19 gb|AAR32679.1| 4-methyl-5(B-hydroxyethyl)-thiazole monophosphate... 96 4e-19 >gb|AAN18034.1| DarS [Pseudomonas aurantiaca] Length = 228 Score = 101 bits (251), Expect = 9e-21, Method: Compositional matrix adjust. Identities = 62/184 (33%), Positives = 98/184 (53%), Gaps = 6/184 (3%) Query: 1 MRIAFVLFDGITFLDFAGFYDVIRRLGNFEAGHGLEMDICGFREEVQDEQGLTVKISKVQ 60 ++I F+LF + LD G YDV+ L + + + RE V GL ++ + Sbjct: 3 LQIGFLLFPNVQQLDLTGPYDVLASLPDVQVHL-----VWKTREAVTASTGLVLQATTSF 57 Query: 61 PELGQYDLLFVPGGMGTRILRNDGPFISWLQ-GAKSVPYKVSVCTGSLLLGAAGFLRDCW 119 + Q D++ +PGG G L D + +++ A Y SVCTGSL+LGAAG LR Sbjct: 58 ADCPQLDVICIPGGSGVGPLMEDAQTLDFIRRQAAQARYVTSVCTGSLVLGAAGLLRGKR 117 Query: 120 ATTHPSSYGLLEPYCGQVIETRIVQDGRMITGGGVSTSIDLGLYMMSLLAGEDAMQAVKK 179 ATTH + + LLE ++ R+V+DG +++GGG++ ID L ++ L Q V+ Sbjct: 118 ATTHWAYHHLLEALGAIAVQERVVRDGNVLSGGGITAGIDFALTLVGELFDAATAQRVQL 177 Query: 180 QIDY 183 Q++Y Sbjct: 178 QLEY 181 >dbj|BAC41251.1| isonitrile hydratase [Pseudomonas putida] Length = 228 Score = 98.2 bits (243), Expect = 9e-20, Method: Compositional matrix adjust. Identities = 64/184 (34%), Positives = 97/184 (52%), Gaps = 6/184 (3%) Query: 1 MRIAFVLFDGITFLDFAGFYDVIRRLGNFEAGHGLEMDICGFREEVQDEQGLTVKISKVQ 60 ++I F+LF + LD G YDV+ L + + H + D+ V GL +K + Sbjct: 3 LQIGFLLFPQVQQLDLTGPYDVLASLPDVQV-HLVWKDLV----PVTSSTGLQLKPTTTF 57 Query: 61 PELGQYDLLFVPGGMGTRILRNDGPFISWLQG-AKSVPYKVSVCTGSLLLGAAGFLRDCW 119 + D++ VPGG G L D + +++ A Y SVCTGSL+LGAAG L+ Sbjct: 58 EDCPVLDVICVPGGAGVGPLMEDEQTLDFIRSQAAQARYVTSVCTGSLVLGAAGLLQGKR 117 Query: 120 ATTHPSSYGLLEPYCGQVIETRIVQDGRMITGGGVSTSIDLGLYMMSLLAGEDAMQAVKK 179 ATTH + + LL ++ R+V+DG + TGGG++ ID L + L G D Q V+ Sbjct: 118 ATTHWAYHDLLPTLGAIPVKDRVVRDGNLFTGGGITAGIDFALTLAQELVGVDTAQLVQL 177 Query: 180 QIDY 183 Q++Y Sbjct: 178 QLEY 181 >ref|ZP_00085238.1| COG0693: Putative intracellular protease/amidase [Pseudomonas fluorescens PfO-1] Length = 240 Score = 97.8 bits (242), Expect = 1e-19, Method: Compositional matrix adjust. Identities = 64/184 (34%), Positives = 97/184 (52%), Gaps = 6/184 (3%) Query: 1 MRIAFVLFDGITFLDFAGFYDVIRRLGNFEAGHGLEMDICGFREEVQDEQGLTVKISKVQ 60 ++I F+LF + LD G YDV+ L + + H + D+ V GL +K + Sbjct: 15 LQIGFLLFPQVQQLDLTGPYDVLASLPDVQV-HLVWKDLV----PVTSSTGLQLKPTTTF 69 Query: 61 PELGQYDLLFVPGGMGTRILRNDGPFISWLQG-AKSVPYKVSVCTGSLLLGAAGFLRDCW 119 + D++ VPGG G L D + +++ A Y SVCTGSL+LGAAG L+ Sbjct: 70 EDCPVLDVICVPGGAGVGPLMEDEQTLDFIRSQAAQARYVTSVCTGSLVLGAAGLLQGKR 129 Query: 120 ATTHPSSYGLLEPYCGQVIETRIVQDGRMITGGGVSTSIDLGLYMMSLLAGEDAMQAVKK 179 ATTH + + LL ++ R+V+DG + TGGG++ ID L + L G D Q V+ Sbjct: 130 ATTHWAYHDLLPTLGAIPVKDRVVRDGNLFTGGGITAGIDFALTLAQELVGVDTAQLVQL 189 Query: 180 QIDY 183 Q++Y Sbjct: 190 QLEY 193 >ref|NP_770920.1| bll4280 [Bradyrhizobium japonicum] dbj|BAC49545.1| bll4280 [Bradyrhizobium japonicum USDA 110] Length = 228 Score = 96.7 bits (239), Expect = 2e-19, Method: Compositional matrix adjust. Identities = 64/185 (34%), Positives = 99/185 (53%), Gaps = 8/185 (4%) Query: 1 MRIAFVLFDGITFLDFAGFYDVIRRLGNFEAGHGLEMDICGFR-EEVQDEQGLTVKISKV 59 ++I ++F +T LDF G V F A G ++ + R E V + LT+ + Sbjct: 5 LQIGLLVFPRVTQLDFTGPLQV------FSAVPGAKLHLIWKRIEPVPSDSVLTLTPTTT 58 Query: 60 QPELGQYDLLFVPGGMGTRILRNDGPFISWLQ-GAKSVPYKVSVCTGSLLLGAAGFLRDC 118 + Q D++ VPGG GT L ND + +L+ A+ Y SVCTG+L+LGAAG L+ Sbjct: 59 FADCPQLDVICVPGGGGTNDLLNDEEVLDFLRRQAEGARYVTSVCTGALVLGAAGLLKGY 118 Query: 119 WATTHPSSYGLLEPYCGQVIETRIVQDGRMITGGGVSTSIDLGLYMMSLLAGEDAMQAVK 178 A TH S+ +L + +TR+ D +TGGGV+ ID L ++S+L +A++ Sbjct: 119 RAATHWSAMEMLGQFGATPTKTRVCIDRNRVTGGGVTAGIDFALTLVSILVDRTTAEAIQ 178 Query: 179 KQIDY 183 QI+Y Sbjct: 179 LQIEY 183 >gb|AAR32679.1| 4-methyl-5(B-hydroxyethyl)-thiazole monophosphate biosynthesis enzyme [Streptomyces hygroscopicus] Length = 270 Score = 96.3 bits (238), Expect = 4e-19, Method: Compositional matrix adjust. Identities = 63/184 (34%), Positives = 94/184 (51%), Gaps = 9/184 (4%) Query: 3 IAFVLFDGITFLDFAGFYDVIRRLGNFEAGHGLEMDICGFRE-EVQDEQG-LTVKISKVQ 60 I L+DG LD G Y+V+ + G + G + V+D G L + Sbjct: 60 IVIPLYDGYEPLDVVGPYEVLAYM------PGASVRFVGAEKGPVKDALGSLPTYVPATY 113 Query: 61 PELGQYDLLFVPGGMGTRILRNDGPFISWLQGAKS-VPYKVSVCTGSLLLGAAGFLRDCW 119 E+ + D+L +PGG + D F++W++ + + SVCTGSL+LGAAG L+ Sbjct: 114 SEVERCDVLLIPGGRSVEAMVADADFLAWVRRIHARTRFTTSVCTGSLVLGAAGLLKGLT 173 Query: 120 ATTHPSSYGLLEPYCGQVIETRIVQDGRMITGGGVSTSIDLGLYMMSLLAGEDAMQAVKK 179 ATTH S LE Y R V+ G++IT GVS+ ID+ L + +LL E QA++ Sbjct: 174 ATTHWSGAEALESYGAAYTPQRTVRQGKVITSAGVSSGIDMALTLAALLTDEVTAQAIQL 233 Query: 180 QIDY 183 I+Y Sbjct: 234 WIEY 237 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.325 0.144 0.439 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 294,868,328 Number of extensions: 12037424 Number of successful extensions: 29626 Number of sequences better than 1.0e-05: 98 Number of HSP's gapped: 29611 Number of HSP's successfully gapped: 98 Length of query: 194 Length of database: 757,237,578 Length adjustment: 119 Effective length of query: 75 Effective length of database: 434,140,797 Effective search space: 32560559775 Effective search space used: 32560559775 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.5 bits) S2: 122 (51.6 bits)