BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0574.0008.p (266 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_488363.1| probable glycosyl transferase [Nostoc sp. PCC 7... 105 1e-21 ref|NP_964889.1| hypothetical protein LJ1033 [Lactobacillus john... 92 9e-18 ref|NP_811783.1| putative glycosyltransferase [Bacteroides theta... 88 2e-16 gb|AAC44018.1| EpsK >gi|1588815|prf||2209356M epsK gene 86 7e-16 gb|AAM94576.1| EpsK [Streptococcus thermophilus] >gi|22316054|gb... 86 7e-16 >ref|NP_488363.1| probable glycosyl transferase [Nostoc sp. PCC 7120] pir||AD2346 hypothetical protein alr4323 [imported] - Nostoc sp. (strain PCC 7120) dbj|BAB76022.1| ORF_ID:alr4323~probable glycosyl transferase [Nostoc sp. PCC 7120] Length = 298 Score = 105 bits (262), Expect = 1e-21, Method: Compositional matrix adjust. Identities = 80/265 (30%), Positives = 129/265 (48%), Gaps = 24/265 (9%) Query: 11 HVDRKSGI---EAQILHREDIHFVKQPVDVQWGHYGQIECILKCFELIKEHGHFDYIHII 67 H+D KS I +A ++ + + FV++ ++ WG + ++ + + FDY+ +I Sbjct: 33 HIDGKSNILPFQANLISNK-VTFVQERRNIFWGEFSIVQATIDLMKTALNKQDFDYLILI 91 Query: 68 SGQDLPLVSNRTIVDFFKEHQGKEFVKHLQLPNEAEMWGCMYRVSVYYPRFLVSRAKAVS 127 SG D PL + + I DFF +++G EF+ ++LPNE + + R+ Y R S V Sbjct: 92 SGSDYPLKNAQYIKDFFSKNKGSEFINLVELPNE-QASKNLNRLYQY--RISFSSKNIVL 148 Query: 128 EIRNRYINLVMSVPM-----LKRSLKHLPDALYKGSNWMSVTGECMEYILEYIRDNPGYV 182 R IN +++ ++SL L Y GS W +++G+ YIL +I NP + Sbjct: 149 RAFRRVINCIINDLFHWQRNYRKSLGELKP--YAGSQWWALSGDACNYILNFIAKNPEIL 206 Query: 183 RLFKNSFCGDEIFFHSIILNSRFKHKVVNEIKRYTDW-DTG-PEF-----PRTLRAED-- 233 + F+ + DE FFH+I+ NS F KV + +T W DT PE+ R + D Sbjct: 207 KFFQTALIPDESFFHTILANSDFLTKVKPNLT-FTKWNDTAHPEYITVEIVRDFKGMDAI 265 Query: 234 YERIRQKGTECFWGRKFDLDVDREI 258 Y E + RKF D D I Sbjct: 266 YNHSIGGYGEVLFARKFAPDSDEVI 290 >ref|NP_964889.1| hypothetical protein LJ1033 [Lactobacillus johnsonii NCC 533] gb|AAS08855.1| hypothetical protein LJ1033 [Lactobacillus johnsonii NCC 533] Length = 300 Score = 92.4 bits (228), Expect = 9e-18, Method: Compositional matrix adjust. Identities = 71/246 (28%), Positives = 119/246 (48%), Gaps = 28/246 (11%) Query: 34 PVDVQWGHYGQIECILKCFELIKEHGHFDYIHIISGQDLPLVSNRTIVDFFKEHQGKEFV 93 P+++ WG Y QIE L F+ +GH+DY H++SG DLPL + I DFF ++ KEF+ Sbjct: 63 PINIFWGDYSQIEAELALFK-AANNGHYDYYHLLSGLDLPLANQDQIHDFFDKNLNKEFI 121 Query: 94 KHLQLPNEAEMWGCMYRVSVYYPRFLVSRAKAVSEIRNRYINLVMSVPMLKRSLKHLPDA 153 + N E+ ++ + ++P + K L +P + ++ D Sbjct: 122 TYSGALNPKELSVRLH--NYHFPNTFRTDNKFKGLYHRIETKLYSKMPQRRINI----DK 175 Query: 154 LYKGSNWMSVTGECMEYILEYIRDNPGYV-RLFKNSFCGDEIFFHSII-LNSRFKHKVVN 211 + GSNW+S+ E + ++ DN + LF N F DE+F I L ++K +V + Sbjct: 176 ISFGSNWVSLDNELVSDLV----DNEDLIYHLFHNGFLVDELFIPIFINLFPKYKKRVYD 231 Query: 212 EIK------------RYTDWDTGPEFPRTLRAEDYERIRQ-KGTECFWGRKFDLDVDREI 258 K RY +W G P T R +DY ++Q K + RKFD ++D +I Sbjct: 232 STKVSDKPDEFQGNLRYINWWDGS--PYTWRLKDYGMLQQAKKKGHLFSRKFDENIDSDI 289 Query: 259 VQDILQ 264 ++ ++ Sbjct: 290 IKKVVN 295 >ref|NP_811783.1| putative glycosyltransferase [Bacteroides thetaiotaomicron VPI-5482] gb|AAO77977.1| putative glycosyltransferase [Bacteroides thetaiotaomicron VPI-5482] Length = 291 Score = 88.2 bits (217), Expect = 2e-16, Method: Compositional matrix adjust. Identities = 63/238 (26%), Positives = 111/238 (46%), Gaps = 10/238 (4%) Query: 27 DIHFVKQPVDVQWGHYGQIECILKCFELIKEHGHFDYIHIISGQDLPLVSNRTIVDFFKE 86 +++ +++ D++WGH QI+ FE +DY H++SG LPL S I FF+ Sbjct: 55 NLYILQKRTDIRWGHISQIKAEYALFEAAFMQDRYDYYHLLSGVHLPLKSQSYIHHFFEG 114 Query: 87 HQGKEFVKHLQLPNEAEMWGCMYRVSVYYPRFLVSRAKAVSEIRNRYINLVMSVPMLKRS 146 + KE + H +PN R ++ + + + + I + + + Sbjct: 115 LKDKEVLVH--VPNSDYQTHLKMRRYNFFTKNFMHKVLFIRRINQLLWRVCIRIQKELHI 172 Query: 147 LKHLPDALYKGSNWMSVTGECMEYILEYIRDNPGYVRLFKNSFCGDEIFFHSIILNSRFK 206 ++ + +NW+S+TG+C+ Y+L+ +D +R ++ + CGDE F S + S K Sbjct: 173 CRNRNQSYTNAANWVSITGKCIGYLLQIKKD---VLRKYRFTLCGDEFFIPSELGKSYLK 229 Query: 207 HKVVNEIKRY-TDWDTGPEFPRTLRAEDYERIRQKGTECFWGRKFDLDVDREIVQDIL 263 ++ + K D+D G PR DY+ + G C + RKF D IV IL Sbjct: 230 DRIYYDDKLLKCDFDGGSN-PRIYHLGDYDELISSG--CLFARKFSY-TDMHIVDKIL 283 >gb|AAC44018.1| EpsK prf||2209356M epsK gene Length = 316 Score = 86.3 bits (212), Expect = 7e-16, Method: Compositional matrix adjust. Identities = 71/266 (26%), Positives = 116/266 (43%), Gaps = 24/266 (9%) Query: 6 FXXXXHVDRKSGIE-----AQILHREDIHFVKQPVDVQWGHYGQIECILKCFELIKEHGH 60 F H+D+KS I+ +I IHF ++ +V WG + +E + E ++ G Sbjct: 32 FDFFLHIDKKSRIQDFFYLKKITKFSTIHFSERK-NVHWGGFSMVEAMFALLECARDTGE 90 Query: 61 FDYIHIISGQDLPLVSNRTIVDFFKEHQGKEFVKHLQLPN---------EAEMWGCMYRV 111 + Y H +SG D+P+ N + +FF+ K F+ L N EM RV Sbjct: 91 YSYFHFLSGDDMPIKDNEIVFNFFENSYPKNFIDILDFENVNKNSYFYEPPEM--IEERV 148 Query: 112 SVYYPRFLVSRAKAVSEIRNRYINLVMSVPMLKRSLKHLPDALYKGSNWMSVTGECMEYI 171 YYP + K + I + I L + + LK+ ++KG W S+T + ++ + Sbjct: 149 KYYYPHMDILNRKGTNFIGKKLIYLQKLLKV--NRLKNREIEIFKGHQWCSLTNQFVDIL 206 Query: 172 LEYIRDNPGYVRLFKNSFCGDEIFFHSIILNSRFK-HKVVNEIKRYTDWDTGPEFPRTLR 230 L+ G F +S DE +F + + + + ++ N R DW G P R Sbjct: 207 LDKEERRVG-KSYFSSSLIPDECYFQTFAMIKKVEIYQQKNMSARLIDWTRGK--PYIWR 263 Query: 231 AEDYERIRQKGTECFWGRKFDLDVDR 256 +D+ I F RKFD +VDR Sbjct: 264 QDDFFEIMNDKDSMF-SRKFDENVDR 288 >gb|AAM94576.1| EpsK [Streptococcus thermophilus] gb|AAL32504.1| CpsK [Streptococcus thermophilus] gb|AAQ04256.1| EpsK [Streptococcus thermophilus] Length = 316 Score = 86.3 bits (212), Expect = 7e-16, Method: Compositional matrix adjust. Identities = 71/266 (26%), Positives = 116/266 (43%), Gaps = 24/266 (9%) Query: 6 FXXXXHVDRKSGIE-----AQILHREDIHFVKQPVDVQWGHYGQIECILKCFELIKEHGH 60 F H+D+KS I+ +I IHF ++ +V WG + +E + E ++ G Sbjct: 32 FDFFLHIDKKSRIQDFFYLKKITKFSTIHFSERK-NVHWGGFSMVEAMFALLECARDTGE 90 Query: 61 FDYIHIISGQDLPLVSNRTIVDFFKEHQGKEFVKHLQLPN---------EAEMWGCMYRV 111 + Y H +SG D+P+ N + +FF+ K F+ L N EM RV Sbjct: 91 YSYFHFLSGDDMPIKDNEIVFNFFENSYPKNFIDILDFENVNKNSYFYEPPEM--IEERV 148 Query: 112 SVYYPRFLVSRAKAVSEIRNRYINLVMSVPMLKRSLKHLPDALYKGSNWMSVTGECMEYI 171 YYP + K + I + I L + + LK+ ++KG W S+T + ++ + Sbjct: 149 KYYYPHMDILNRKGTNFIGKKLIYLQKLLKV--NRLKNREIEIFKGHQWCSLTNQFVDIL 206 Query: 172 LEYIRDNPGYVRLFKNSFCGDEIFFHSIILNSRFK-HKVVNEIKRYTDWDTGPEFPRTLR 230 L+ G F +S DE +F + + + + ++ N R DW G P R Sbjct: 207 LDKEERRVG-KSYFSSSLIPDECYFQTFAMIKKVEIYQQKNMSARLIDWTRGK--PYIWR 263 Query: 231 AEDYERIRQKGTECFWGRKFDLDVDR 256 +D+ I F RKFD +VDR Sbjct: 264 QDDFFEIMNDKDSMF-SRKFDENVDR 288 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.326 0.142 0.453 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 409,132,818 Number of extensions: 16792592 Number of successful extensions: 43496 Number of sequences better than 1.0e-05: 9 Number of HSP's gapped: 43500 Number of HSP's successfully gapped: 10 Length of query: 266 Length of database: 757,237,578 Length adjustment: 123 Effective length of query: 143 Effective length of database: 423,280,401 Effective search space: 60529097343 Effective search space used: 60529097343 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 125 (52.8 bits)