BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0574.0001.p (127 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_00097833.1| COG1247: Sortase and related acyltransferases... 65 6e-10 ref|NP_721464.1| putative acetyltransferase [Streptococcus mutan... 62 5e-09 ref|NP_385668.1| PUTATIVE ACETYLTRANSFERASE (ANTIBIOTIC RESISTAN... 60 2e-08 ref|ZP_00170037.1| hypothetical protein Raeut127201 [Ralstonia e... 59 4e-08 ref|NP_539296.1| PHOSPHINOTHRICIN N-ACETYLTRANSFERASE [Brucella ... 58 7e-08 >ref|ZP_00097833.1| COG1247: Sortase and related acyltransferases [Desulfitobacterium hafniense] Length = 166 Score = 64.7 bits (156), Expect = 6e-10, Method: Compositional matrix adjust. Identities = 32/114 (28%), Positives = 56/114 (49%) Query: 9 DPRFKSYAILKNGEMQGYVLIARHKNKQAYDSSGEISVYXXXXXXXXXXXXXXXSFIEER 68 DP + Y + G++ G+ ++ + AY SGE S+Y + + Sbjct: 52 DPYYPVYVVENQGQVTGWGSLSPFHPRAAYKQSGEFSIYIAPEWTGQSMGDALLKVLCQE 111 Query: 69 AAELGFHVLVATVCADNEPSRRLFTRHGYEQSALFKEIGCKFGRWLDIASYQKI 122 A +LG+H L+ + + NE S L +HG+ ++ + E+G KFG+ LD+ Q I Sbjct: 112 AKKLGYHTLLGRITSTNEKSISLAKKHGFWEAGKYHEVGTKFGQCLDVMIMQII 165 >ref|NP_721464.1| putative acetyltransferase [Streptococcus mutans UA159] gb|AAN58770.1| putative acetyltransferase [Streptococcus mutans UA159] Length = 163 Score = 61.6 bits (148), Expect = 5e-09, Method: Compositional matrix adjust. Identities = 34/103 (33%), Positives = 49/103 (47%) Query: 19 KNGEMQGYVLIARHKNKQAYDSSGEISVYXXXXXXXXXXXXXXXSFIEERAAELGFHVLV 78 + G +QGY + + N AYD + E+S+Y + +E E GF L+ Sbjct: 57 EEGILQGYAYASVYYNHAAYDWTVELSIYIKEEARGKHIGSQLYASLERNLQEAGFVNLL 116 Query: 79 ATVCADNEPSRRLFTRHGYEQSALFKEIGCKFGRWLDIASYQK 121 + N S +HGYEQ A FK++G KF +W DI QK Sbjct: 117 VCIALPNNASLTFHKKHGYEQVAHFKKVGYKFDKWYDIVWMQK 159 >ref|NP_385668.1| PUTATIVE ACETYLTRANSFERASE (ANTIBIOTIC RESISTANCE ) PROTEIN [Sinorhizobium meliloti] emb|CAC46141.1| PUTATIVE ACETYLTRANSFERASE (ANTIBIOTIC RESISTANCE ) PROTEIN [Sinorhizobium meliloti] Length = 169 Score = 59.7 bits (143), Expect = 2e-08, Method: Compositional matrix adjust. Identities = 36/111 (32%), Positives = 57/111 (51%), Gaps = 1/111 (0%) Query: 16 AILKNGEMQGYVLIARHKNKQAYDSSGEISVYXXXXXXXXXXXXXXXSFIEERAAELGFH 75 A+ +GE+ GY + Y + E SVY + +RA LG H Sbjct: 56 ALSLDGEVVGYASFGEWRAFDGYRHTVEHSVYVRADQRGGGIGRALMLELIDRAEALGKH 115 Query: 76 VLVATVCADNEPSRRLFTRHGYEQSALFKEIGCKFGRWLDIASYQKIIGRT 126 V++A + + N PS RL + G+ ++ KE+G KFGRWLD+ ++ ++I RT Sbjct: 116 VMIAGIESGNLPSIRLHEQLGFRETGHMKEVGTKFGRWLDL-TFMQLILRT 165 >ref|ZP_00170037.1| hypothetical protein Raeut127201 [Ralstonia eutropha JMP134] Length = 73 Score = 58.5 bits (140), Expect = 4e-08, Method: Compositional matrix adjust. Identities = 27/54 (50%), Positives = 35/54 (64%) Query: 67 ERAAELGFHVLVATVCADNEPSRRLFTRHGYEQSALFKEIGCKFGRWLDIASYQ 120 +RA ELG HV+VA + A N S RL + G+ Q L E+G KFG+WLD+A Q Sbjct: 5 DRARELGKHVMVAAIEAQNASSIRLHEKLGFRQVGLLPEVGTKFGKWLDLAFLQ 58 >ref|NP_539296.1| PHOSPHINOTHRICIN N-ACETYLTRANSFERASE [Brucella melitensis] pir||AE3299 phosphinothricin N-acetyltransferase (EC 2.3.1.-) [imported] - Brucella melitensis (strain 16M) gb|AAL51560.1| PHOSPHINOTHRICIN N-ACETYLTRANSFERASE [Brucella melitensis 16M] Length = 164 Score = 57.8 bits (138), Expect = 7e-08, Method: Compositional matrix adjust. Identities = 33/105 (31%), Positives = 51/105 (48%) Query: 19 KNGEMQGYVLIARHKNKQAYDSSGEISVYXXXXXXXXXXXXXXXSFIEERAAELGFHVLV 78 + G++ GY + + + S E+SVY + + E A E HVL+ Sbjct: 60 REGQVVGYASYGPFRPFEGFRHSSELSVYVASNARGGGIGRTLLAELIEEARERKVHVLI 119 Query: 79 ATVCADNEPSRRLFTRHGYEQSALFKEIGCKFGRWLDIASYQKII 123 A++ A N S L G+E+ K++G KFGRWLD+ QKI+ Sbjct: 120 ASIEAGNAASIALHRSQGFEECGTLKQVGQKFGRWLDLLFMQKIL 164 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.322 0.137 0.406 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 161,357,304 Number of extensions: 4610822 Number of successful extensions: 7347 Number of sequences better than 1.0e-05: 21 Number of HSP's gapped: 7368 Number of HSP's successfully gapped: 21 Length of query: 127 Length of database: 757,237,578 Length adjustment: 90 Effective length of query: 37 Effective length of database: 512,878,668 Effective search space: 18976510716 Effective search space used: 18976510716 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 120 (50.8 bits)