BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0571.0029.p (338 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_624109.1| putative membrane protein [Thermoanaerobacter t... 132 1e-29 ref|NP_625193.1| putative membrane protein. [Streptomyces coelic... 116 1e-24 ref|NP_766754.1| bll0114 [Bradyrhizobium japonicum] >gi|27348361... 112 1e-23 ref|ZP_00170398.1| hypothetical protein Raeut166001 [Ralstonia e... 99 1e-19 ref|ZP_00181079.1| hypothetical protein Mther098301 [Moorella th... 97 6e-19 >ref|NP_624109.1| putative membrane protein [Thermoanaerobacter tengcongensis] gb|AAM25713.1| putative membrane protein [Thermoanaerobacter tengcongensis] Length = 448 Score = 132 bits (332), Expect = 1e-29, Method: Compositional matrix adjust. Identities = 89/288 (30%), Positives = 137/288 (47%), Gaps = 27/288 (9%) Query: 11 DNGKVTALITASKNGLQALQAKVYIDCTGDADVAAWAGAEYLKGDTQTGEMMPATHCFTL 70 D K+ ++ +K+G +++K+YID TGDAD+A G +YL G + P T + Sbjct: 130 DGSKIKSIKIVNKSGEMTIKSKIYIDSTGDADLAVRCGVDYLLGRPKDNLTQPMTMNVRI 189 Query: 71 GNVD-----EYAYLNGPLLHSNNKTSPI----------YDILK----SGCYPLIPDAHIC 111 GNVD EY N +K I Y ILK G D + Sbjct: 190 GNVDIQKIKEYMINNPTEFRMVDKEKIIKAKRLSVNGFYSILKEARKKGEINFERDMVLF 249 Query: 112 NNMIAPRTVGFNAGHLWEVDNTDAGSISEALMQGRKLAAAYRDALAEFIPASFGSSYVAN 171 P V N + ++ TD +++ + GR A + + + IP F +S + + Sbjct: 250 FETNTPGEVIVNMTRVQRLNGTDIKDLTKGEIIGRNQAIELFNFMKKRIPG-FENSILIS 308 Query: 172 TGSLLGVRETRRITGDYVLSVEDYVARRSFADEICRNSYFIDIHGTEKEEKQAGGKPEVI 231 TG +GVRETR+I G+Y+L+ ED V+ + F D I + Y +DIH E G+ V Sbjct: 309 TGPQIGVRETRKIIGEYILTAEDLVSNKRFDDCIAKGGYPVDIHSPE-------GEGVVY 361 Query: 232 KRYGPGESHGIPYRCLTPRSLKNVLVAGRSISCVREVQGSVRVMPVCL 279 + G + IPYR L + + N+LV+GR IS E ++RV P+ + Sbjct: 362 MKLKKGSDYDIPYRSLYSKKVDNLLVSGRCISSTHEANAAIRVSPIAM 409 >ref|NP_625193.1| putative membrane protein. [Streptomyces coelicolor A3(2)] emb|CAB62685.1| putative membrane protein. [Streptomyces coelicolor A3(2)] Length = 478 Score = 116 bits (290), Expect = 1e-24, Method: Compositional matrix adjust. Identities = 91/284 (32%), Positives = 132/284 (46%), Gaps = 21/284 (7%) Query: 5 GAVDTDDNGKVTALITASKNGLQALQAKVYIDCTGDADVAAWAGAEYLKGDTQTGEMMPA 64 GA+ DD G ++ +K+G + A V +D TGD DVAA GA Y G + G + P Sbjct: 163 GALPLDD-GPGWRVVFETKSGPVVIDAGVVVDGTGDGDVAAACGAPYEVGRPEDGLVQPM 221 Query: 65 THCFTLGNV---DEYAYLNGPLLHSNN--KTSPIYDIL----KSGCYPLIPDAHICNNMI 115 T F + + D AY+ H + ++D++ K+G L + + Sbjct: 222 TLMFRVADFARPDFAAYVRE---HPDQWRGVHGLWDLIEEARKNGELRLPREDILFFATP 278 Query: 116 APRTVGFNAGHLWEVDNTDAGSISEALMQGRKLAAAYRDALAEFIPASFGSSYVANTGSL 175 P V N+ + V T ++ A R A L +P F SYV +G+ Sbjct: 279 HPHEVAVNSTRVNRVLGTSVWDLTRAEYTARHQLAEIDRFLRTRVPG-FEESYVVQSGTH 337 Query: 176 LGVRETRRITGDYVLSVEDYVARRSFADEICRNSYFIDIHGTEKEEKQAGGKPEVIKRYG 235 +GVRE+RR+ GD+ L+ D +A R+F D I +Y +DIH G V+KR Sbjct: 338 VGVRESRRVVGDHRLTGHDILAARTFPDVIAHGAYPVDIHNPR-------GSGTVLKRVP 390 Query: 236 PGESHGIPYRCLTPRSLKNVLVAGRSISCVREVQGSVRVMPVCL 279 G + IP RCL PR +LVAGR IS S RVMP+ + Sbjct: 391 RGSFYDIPLRCLLPRDTDRLLVAGRCISGTHVAHSSYRVMPIAM 434 >ref|NP_766754.1| bll0114 [Bradyrhizobium japonicum] dbj|BAC45379.1| bll0114 [Bradyrhizobium japonicum USDA 110] Length = 454 Score = 112 bits (280), Expect = 1e-23, Method: Compositional matrix adjust. Identities = 89/285 (31%), Positives = 133/285 (46%), Gaps = 25/285 (8%) Query: 4 LGA-VDTDDNGKVTALITASKNGLQALQAKVYIDCTGDADVAAWAGAEYLKGDTQTGEMM 62 LGA V DN ++ AL+ +K G QA++++++IDC+GD D+A WAGA + GD + Sbjct: 137 LGAGVVMGDNSRIDALMVETKAGRQAVRSEIFIDCSGDGDLAVWAGAPFEVGDEHGHPLY 196 Query: 63 PATHCFTLGNVDEY----AYLNGPLLHSNNKTSPIYDILKSGCYPLIPDAHICNNMIAPR 118 P+ L N+D A+ P L + + + + P + Sbjct: 197 PSM-MLRLNNIDPEKAGEAWRTIPQLMEKALAAGTHKFPRKSAI-VRPQKSGIEWRVNFT 254 Query: 119 TVGFNAGHLWEVDNTDAGSISEALMQGRKLAAAYRDALAEFIPASFGSSYVANTGSLLGV 178 V GH ++ + ++ ++GR+ A A + L +P F SY+ + LG+ Sbjct: 255 QVAREDGH--AINGIEPDDLTRGEIEGRRQALAAFEFL-RTVPG-FEKSYIVDLPPQLGI 310 Query: 179 RETRRITGDYVLSVEDYVARRSFADEICRNSYFIDIHGTEKEEKQAGGKPEVIKRYGP-G 237 RETRRI G Y LS ED + SF D I N + I+ H +VI + P Sbjct: 311 RETRRIKGGYQLSGEDVLGCASFEDSIGVNGWPIEAHVPG----------DVIFTFPPIP 360 Query: 238 ESHG---IPYRCLTPRSLKNVLVAGRSISCVREVQGSVRVMPVCL 279 ES G +PYR L P + N+LVAGR S E Q + RV C Sbjct: 361 ESRGYNELPYRMLVPEGVDNLLVAGRCASMTHEGQSAARVSGACF 405 >ref|ZP_00170398.1| hypothetical protein Raeut166001 [Ralstonia eutropha JMP134] Length = 411 Score = 99.0 bits (245), Expect = 1e-19, Method: Compositional matrix adjust. Identities = 81/274 (29%), Positives = 128/274 (46%), Gaps = 29/274 (10%) Query: 14 KVTALITASKNGLQALQAKVYIDCTGDADVAAWAGA--EYLKGDTQTGEMMPATHC-FTL 70 ++ A++ +K G A++AK ++DC+GDAD+ ++AGA E +GD Q P T F Sbjct: 116 ELDAVLIETKEGRLAIRAKSFVDCSGDADLVSFAGAPCEISEGDLQ----FPTTMVRFGG 171 Query: 71 GNVDEYAYLNGPLLHS--NNKTSPIYDILKS--GCYPLIPD-AHICNNMIAPRTVGFNAG 125 + D A L+ P L + YD+ ++ G + P AH+ +A N Sbjct: 172 VDTDSLARLSRPALRERLEQAVADGYDLPRTAGGVFAERPGLAHLNITKVAVDGKSPNP- 230 Query: 126 HLWEVDNTDAGSISEALMQGRKLAAAYRDALAEFIPASFGSSYVANTGSLLGVRETRRIT 185 D +S A + GR+ Y A +++P F S++V +TG+++G+RE+RR Sbjct: 231 -------FDLSVMSRAEIAGREQVCTYLKAFRKYVPG-FESAFVMDTGAVIGIRESRRTV 282 Query: 186 GDYVLSVEDYVARRSFADEICRNSYFIDIHGTEKEEKQAGGKPEVIKRYGPGESHGIPYR 245 G YVL+ D + F D I ++ ++ H A G+ PG IP R Sbjct: 283 GSYVLTDRDVLEGARFDDAIACCAWPMEDH--------AAGRATKWIWLPPGTYFQIPLR 334 Query: 246 CLTPRSLKNVLVAGRSISCVREVQGSVRVMPVCL 279 L N++VAGR S Q S+RV C Sbjct: 335 SLLLEGFDNLMVAGRCASATHGAQASIRVTAQCF 368 >ref|ZP_00181079.1| hypothetical protein Mther098301 [Moorella thermoacetica ATCC 39073] Length = 662 Score = 97.1 bits (240), Expect = 6e-19, Method: Compositional matrix adjust. Identities = 87/276 (31%), Positives = 118/276 (42%), Gaps = 27/276 (9%) Query: 19 ITASKNGLQ-ALQAKVYIDCTGDADVAAWAGAEYLKGDTQTGEM---MPATHCFTLGNVD 74 I A K+G + A ID T +ADVAA AG Y G GE T F LG VD Sbjct: 205 IKAVKDGRELPFYASRVIDATQNADVAAAAGVPYTVGAEDIGEKDRRQAVTLVFRLGGVD 264 Query: 75 EYAYLNGPLLHSNNKTSPIYD-------ILKSGCYPLIPDAHICNNMIAPR---TVGFNA 124 A + S K + I D + G P P + IA + +V NA Sbjct: 265 WQALARA--VGSQIKDAKISDRAAWGFGSIARGYQPSTPRLRLRGFNIARQDDGSVFINA 322 Query: 125 GHLWEVDNTDAGSISEALMQGRKLAAAYRDALAEFIPASFGSSYVANTGSLLGVRETRRI 184 ++ VD A S EA+ ++ A D L +P F + + L +RETR I Sbjct: 323 LQIFGVDGLSAASREEAIKLAQRELPAITDFLRSHMPG-FAGARLLGAAPELYIRETRHI 381 Query: 185 TGDYVLSVEDYVARRSFADEICRNSYFIDIHGTEKEEK-QAGGKPEVIKRYGPGESHGIP 243 Y L + D + R F D I SY +D+ T E+ G+PEV + IP Sbjct: 382 KALYQLDLNDVLFNRYFPDAIALGSYPVDVQATSPEDTGYVYGRPEV---------YSIP 432 Query: 244 YRCLTPRSLKNVLVAGRSISCVREVQGSVRVMPVCL 279 +R L P + N+LV GRS GS RV+P+ + Sbjct: 433 FRSLVPEKIDNLLVVGRSAGYTHLAAGSARVVPIGM 468 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.318 0.135 0.409 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 458,198,054 Number of extensions: 19018772 Number of successful extensions: 36053 Number of sequences better than 1.0e-05: 29 Number of HSP's gapped: 36022 Number of HSP's successfully gapped: 31 Length of query: 338 Length of database: 757,237,578 Length adjustment: 126 Effective length of query: 212 Effective length of database: 415,135,104 Effective search space: 88008642048 Effective search space used: 88008642048 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 126 (53.1 bits)