BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0571.0018.p (421 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gb|AAR10414.1| putative multidrug efflux protein [Enterococcus f... 243 9e-63 ref|NP_977251.1| multidrug resistance protein [Bacillus cereus A... 240 5e-62 ref|NP_242641.1| multidrug-efflux transporter [Bacillus halodura... 240 5e-62 ref|NP_654778.1| sugar_tr, Sugar (and other) transporter [Bacill... 238 2e-61 ref|NP_830641.1| Multidrug resistance protein B [Bacillus cereus... 236 7e-61 >gb|AAR10414.1| putative multidrug efflux protein [Enterococcus faecium] Length = 395 Score = 243 bits (619), Expect = 9e-63, Method: Compositional matrix adjust. Identities = 134/372 (36%), Positives = 197/372 (52%), Gaps = 6/372 (1%) Query: 35 RILAILMINIFIVMLGVGLITPILPKLITEFGAGGQSIGFLVAAYGLTQFLLSPITGQLS 94 + L +LM+ +FI GLI PILP + +F GQ +G LVA YG+ Q LSPI G+ + Sbjct: 9 KTLFLLMVCVFIATAAFGLIIPILPDFMGKFNTNGQMMGLLVATYGIIQLFLSPIAGRFA 68 Query: 95 DRYGRKTFIITGXXXXXXXXXXXXXGNALWMLYTSRLLEGVAAALIIPTMMACVADITTT 154 DRYGRK I G W+L+ R L G+A +L+IP MAC+ DITT Sbjct: 69 DRYGRKRIIEIGLICLTLSQLVFAFSVHFWLLFLGRFLTGIAVSLLIPGAMACIIDITTE 128 Query: 155 EERAKGNSLLAAAMSXXXXXXXXXXXXXXXXXTRVPLYXXXXXXXXXXXFSIFCLPESIS 214 EERAKG S L A++S VP Y S F LPE++ Sbjct: 129 EERAKGLSFLNASISFGFVIGPGIGGFLTTYGLYVPFYFATVLSFVSFLLSFFLLPETLE 188 Query: 215 KEQMNAARAKVAPKESIFEQYAKSLRSKYVMLFALVLVMTFGLANFETVFGLYVTNRFQF 274 K+ V+P+ + +Q +S+R YV+ LV + + L FE +FGL+V +F + Sbjct: 189 KKTEMTKADTVSPQPMV-QQILRSIRVPYVVPLLLVFLYSVSLYIFEAIFGLFVAKQFGY 247 Query: 275 TPQNISIILTAGAVIGVGMQALVVAKVIKIFGEQKVIKGSLLIISXXXXXXXXXXXXXXX 334 T ++I++++T A + V +Q L+ K++ +FGE KV+ G+++ Sbjct: 248 TAKDIAMVITIAAFVSVMVQLLLTNKLVSLFGESKVMNGTMI-----AGGVSFLFLLFTT 302 Query: 335 XXXXXXXXXXXXFFANAMLRPALNTLLSKRAGTEQGYAAGMNNAYMSVGNIAGPTLAGFL 394 + A ++LRP +NT+LSK A EQG+ AGMNNAY+S+G++ GP L G L Sbjct: 303 RIWSILLLTCLLYTATSILRPVINTVLSKLANNEQGFIAGMNNAYVSLGSVVGPILGGIL 362 Query: 395 FDINMFAPFIAG 406 FDIN + P++ G Sbjct: 363 FDINTYFPYVIG 374 >ref|NP_977251.1| multidrug resistance protein [Bacillus cereus ATCC 10987] gb|AAS39859.1| multidrug resistance protein [Bacillus cereus ATCC 10987] Length = 400 Score = 240 bits (612), Expect = 5e-62, Method: Compositional matrix adjust. Identities = 141/375 (37%), Positives = 204/375 (54%), Gaps = 8/375 (2%) Query: 30 LTNNKRILAILMINIFIVMLGVGLITPILPKLITEFGAGGQSIGFLVAAYGLTQFLLSPI 89 + K +L IL+ NIFI LG+GLI P++P + + G G+++G+LVA + + Q + SPI Sbjct: 5 IKEQKVVLVILLSNIFIAFLGIGLIIPVMPSFMNDMGLTGKTMGYLVAVFAMAQLIASPI 64 Query: 90 TGQLSDRYGRKTFIITGXXXXXXXXXXXXXGNALWMLYTSRLLEGVAAALIIPTMMACVA 149 TG+ D YGRK II G G +WMLY +R+L G++AA I+P + A VA Sbjct: 65 TGRWVDLYGRKKMIIIGLFIFGVSELLFGLGTDVWMLYAARVLGGISAAFIMPGVTAYVA 124 Query: 150 DITTTEERAKGNSLLAAAMSXXXXXXXXXXXXXXXXXTRVPLYXXXXXXXXXXXFSIFCL 209 DIT+ +ER K L+AA+S RVP + SIF L Sbjct: 125 DITSMQERPKAMGYLSAAISTGFIIGPGIGGFIAEYGIRVPFFVAAAIAFIACVISIFIL 184 Query: 210 PESISKEQMNAARAKVAPKESIF-EQYAKSLRSKYVMLFALVLVMTFGLANFETVFGLYV 268 E ++KE++ A KES F KSL Y + F +V V+ FGL+ +ETVF L+ Sbjct: 185 KEPLTKEEL--AEISSNTKESSFIGDLKKSLHPMYAIAFIIVFVLAFGLSAYETVFSLFS 242 Query: 269 TNRFQFTPQNISIILTAGAVIGVGMQALVVAKVIKIFGEQKVIKGSLLIISXXXXXXXXX 328 ++F FTP++I+ I+T ++ GV +Q + K++ IFGE+ +I+ L++ Sbjct: 243 DHKFGFTPKDIAAIITISSIFGVVVQVFMFGKLVDIFGEKVLIQICLIV-----GAVLAF 297 Query: 329 XXXXXXXXXXXXXXXXXXFFANAMLRPALNTLLSKRAGTEQGYAAGMNNAYMSVGNIAGP 388 F A +LRPAL T LSK AG EQG+ AGMN+ Y S+GNIAGP Sbjct: 298 VSTVVFNYWIVLLVTCFIFLAFDLLRPALTTFLSKAAGKEQGFVAGMNSTYTSLGNIAGP 357 Query: 389 TLAGFLFDINMFAPF 403 + G LFD+N+ P+ Sbjct: 358 AMGGILFDMNIHYPY 372 >ref|NP_242641.1| multidrug-efflux transporter [Bacillus halodurans] pir||G83871 multidrug-efflux transporter blt [imported] - Bacillus halodurans (strain C-125) dbj|BAB05494.1| multidrug-efflux transporter [Bacillus halodurans] Length = 409 Score = 240 bits (612), Expect = 5e-62, Method: Compositional matrix adjust. Identities = 138/380 (36%), Positives = 208/380 (54%), Gaps = 7/380 (1%) Query: 31 TNNKRILAILMINIFIVMLGVGLITPILPKLITEFGAGGQSIGFLVAAYGLTQFLLSPIT 90 + + + +LMIN+FI + G+I PILP + G GG + G ++A + Q ++SP+ Sbjct: 3 SEQRHKMILLMINMFIAIASFGIIIPILPAYLESIGEGGTAAGLMIAIFAGAQLVMSPVA 62 Query: 91 GQLSDRYGRKTFIITGXXXXXXXXXXXXXGNALWMLYTSRLLEGVAAALIIPTMMACVAD 150 G+ +D+YGR+ II G +++W LY SR++ GV AAL+IP + A VAD Sbjct: 63 GKWTDQYGRRYMIILGLGGLTLSMFVFYFFDSIWWLYASRVVGGVGAALLIPAIFAYVAD 122 Query: 151 ITTTEERAKGNSLLAAAMSXXXXXXXXXXXXXXXXXTRVPLYXXXXXXXXXXXFSIFCLP 210 ITT +ERAKGNSL++AAMS ++P+ FSI L Sbjct: 123 ITTIDERAKGNSLVSAAMSLGFVVGPGIGGFLADFGLKMPILLSAILSLVAVLFSIMLLK 182 Query: 211 ESISKEQMNAARAKVAPKESIFEQYAKSLRSKYVMLFALVLVMTFGLANFETVFGLYVTN 270 ES ++E +V+ + + +Q +S++ Y + + LVM+FGL +E++ GL+V N Sbjct: 183 ESQTEEAKELTAEQVS-ESPLVKQLIQSVKKPYFIPLVITLVMSFGLMAYESILGLFVDN 241 Query: 271 RFQFTPQNISIILTAGAVIGVGMQALVVAKVIKIFGEQKVIKGSLLIISXXXXXXXXXXX 330 +F TP I+I+LT+ I V +Q VV ++++ GE+ V+ L + S Sbjct: 242 QFGATPGQIAILLTSIGTISVIVQLFVVERLVRWLGERTVLSLFLGVASFGFLLSLFAPS 301 Query: 331 XXXXXXXXXXXXXXXXFFANAMLRPALNTLLSKRAGTEQGYAAGMNNAYMSVGNIAGPTL 390 F A ++LRP L TL+SK AG EQG+A GMNNAYMS+GN+ GP L Sbjct: 302 YGMFFAVTMFI-----FLATSILRPVLTTLISKLAGNEQGFAMGMNNAYMSIGNVLGPAL 356 Query: 391 AGFLFDINMFAPFIAGCCII 410 AG LFD+ + PFI C I+ Sbjct: 357 AGVLFDVQITYPFIL-CLIV 375 >ref|NP_654778.1| sugar_tr, Sugar (and other) transporter [Bacillus anthracis A2012] ref|NP_843352.1| multidrug resistance protein [Bacillus anthracis str. Ames] gb|AAP24838.1| multidrug resistance protein [Bacillus anthracis str. Ames] Length = 400 Score = 238 bits (607), Expect = 2e-61, Method: Compositional matrix adjust. Identities = 140/375 (37%), Positives = 204/375 (54%), Gaps = 8/375 (2%) Query: 30 LTNNKRILAILMINIFIVMLGVGLITPILPKLITEFGAGGQSIGFLVAAYGLTQFLLSPI 89 + K +L IL+ NIFI LG+GLI P++P + + G+++G+LVA + + Q + SPI Sbjct: 5 IKEQKMVLVILLSNIFIAFLGIGLIIPVMPSFMNDMHLTGKTMGYLVAVFAMAQLIASPI 64 Query: 90 TGQLSDRYGRKTFIITGXXXXXXXXXXXXXGNALWMLYTSRLLEGVAAALIIPTMMACVA 149 TG+ D YGRK II G G +WMLY +R+L G++AA I+P + A VA Sbjct: 65 TGRWVDLYGRKKMIIIGLFIFGVSELLFGLGTDVWMLYVARVLGGISAAFIMPGVTAYVA 124 Query: 150 DITTTEERAKGNSLLAAAMSXXXXXXXXXXXXXXXXXTRVPLYXXXXXXXXXXXFSIFCL 209 DIT+ +ER K L+AA+S RVPL+ SIF L Sbjct: 125 DITSIQERPKAMGYLSAAISTGFIIGPGIGGFIAEYGIRVPLFVAAAIAFIACVISIFIL 184 Query: 210 PESISKEQMNAARAKVAPKESIF-EQYAKSLRSKYVMLFALVLVMTFGLANFETVFGLYV 268 E ++KE++ A KES F KSL Y + F +V V+ FGL+ +ETVF L+ Sbjct: 185 KEPLTKEEL--AEISSNTKESSFIGDLKKSLHPMYAIAFIIVFVLAFGLSAYETVFSLFS 242 Query: 269 TNRFQFTPQNISIILTAGAVIGVGMQALVVAKVIKIFGEQKVIKGSLLIISXXXXXXXXX 328 ++F FTP++I+ I+T ++ GV +Q + K++ +FGE+ +I+ L++ Sbjct: 243 DHKFGFTPKDIAAIITISSIFGVVVQVFMFGKLVDMFGEKVLIQICLIV-----GAVLAF 297 Query: 329 XXXXXXXXXXXXXXXXXXFFANAMLRPALNTLLSKRAGTEQGYAAGMNNAYMSVGNIAGP 388 F A +LRPAL T LSK AG EQG+ AGMN+ Y S+GNIAGP Sbjct: 298 VSTVVFNYWIVLLVTCFIFLAFDLLRPALTTFLSKAAGKEQGFVAGMNSTYTSLGNIAGP 357 Query: 389 TLAGFLFDINMFAPF 403 + G LFD+N+ P+ Sbjct: 358 AMGGILFDMNIHYPY 372 >ref|NP_830641.1| Multidrug resistance protein B [Bacillus cereus ATCC 14579] gb|AAP07842.1| Multidrug resistance protein B [Bacillus cereus ATCC 14579] Length = 400 Score = 236 bits (602), Expect = 7e-61, Method: Compositional matrix adjust. Identities = 138/375 (36%), Positives = 203/375 (54%), Gaps = 8/375 (2%) Query: 30 LTNNKRILAILMINIFIVMLGVGLITPILPKLITEFGAGGQSIGFLVAAYGLTQFLLSPI 89 + K +L IL+ NIFI LG+GLI P++P + + G+++G+LVA + + Q + SPI Sbjct: 5 IKEQKMVLVILLSNIFIAFLGIGLIIPVMPSFMNDMHLTGKTMGYLVAVFAMAQLITSPI 64 Query: 90 TGQLSDRYGRKTFIITGXXXXXXXXXXXXXGNALWMLYTSRLLEGVAAALIIPTMMACVA 149 TG+ D YGRK II G G +WMLY +R+L G++AA I+P + A VA Sbjct: 65 TGRWVDLYGRKKMIIIGLFIFGVSELLFGLGKDVWMLYVARVLSGISAAFIMPGVTAYVA 124 Query: 150 DITTTEERAKGNSLLAAAMSXXXXXXXXXXXXXXXXXTRVPLYXXXXXXXXXXXFSIFCL 209 DIT+ +ER K L+AA+S RVP + SIF L Sbjct: 125 DITSIQERPKAMGYLSAAISTGFIIGPGIGGFIAEYGIRVPFFVAAAIAFIACVISIFIL 184 Query: 210 PESISKEQMNAARAKVAPKESIF-EQYAKSLRSKYVMLFALVLVMTFGLANFETVFGLYV 268 E ++KE++ A KES F KSL Y + F +V V+ FGL+ +ETVF L+ Sbjct: 185 KEPLTKEEL--AEISSNTKESSFIGDLKKSLHPMYAIAFIIVFVLAFGLSAYETVFSLFS 242 Query: 269 TNRFQFTPQNISIILTAGAVIGVGMQALVVAKVIKIFGEQKVIKGSLLIISXXXXXXXXX 328 ++F F+P++I+ I+T ++ GV +Q + K++ +FGE+ +I+ L++ Sbjct: 243 DHKFGFSPKDIAAIITISSIFGVVVQVFMFGKLVDMFGEKVLIQICLIV-----GAVLAF 297 Query: 329 XXXXXXXXXXXXXXXXXXFFANAMLRPALNTLLSKRAGTEQGYAAGMNNAYMSVGNIAGP 388 F A +LRPAL T LSK AG EQG+ AGMN+ Y S+GNIAGP Sbjct: 298 VSTVVFNYWIVLLVTCFIFLAFDLLRPALTTFLSKAAGKEQGFVAGMNSTYTSLGNIAGP 357 Query: 389 TLAGFLFDINMFAPF 403 + G LFD+N+ P+ Sbjct: 358 AMGGILFDMNIHYPY 372 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.327 0.140 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 443,052,827 Number of extensions: 15781818 Number of successful extensions: 57033 Number of sequences better than 1.0e-05: 339 Number of HSP's gapped: 57333 Number of HSP's successfully gapped: 351 Length of query: 421 Length of database: 757,237,578 Length adjustment: 128 Effective length of query: 293 Effective length of database: 409,704,906 Effective search space: 120043537458 Effective search space used: 120043537458 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 127 (53.5 bits)