BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0571.0014.p (285 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_241931.1| BH1065~unknown conserved protein [Bacillus halo... 320 2e-86 gb|AAC98126.1| integral membrane protein [Geobacillus stearother... 293 2e-78 ref|NP_693011.1| hypothetical protein OB2090 [Oceanobacillus ihe... 239 7e-62 ref|NP_242978.1| transmembrane lipoprotein [Bacillus halodurans]... 228 1e-58 ref|ZP_00035516.1| COG4209: ABC-type polysaccharide transport sy... 225 8e-58 >ref|NP_241931.1| BH1065~unknown conserved protein [Bacillus halodurans] pir||A83783 hypothetical protein BH1065 [imported] - Bacillus halodurans (strain C-125) dbj|BAB04784.1| BH1065~unknown conserved protein [Bacillus halodurans] Length = 317 Score = 320 bits (821), Expect = 2e-86, Method: Compositional matrix adjust. Identities = 151/285 (52%), Positives = 214/285 (75%) Query: 1 MLLPAVIITIIFRYVPIGGLSVAFMDYQTVFGFFDQEWVGWDNFTYIFNLPGFYNVLWNT 60 +L+PA+++ +IF+Y P+ G+ +AF DY+ GF EWVG +F +F V+WNT Sbjct: 33 LLIPALVLALIFQYGPMFGIVMAFQDYKPWLGFTGSEWVGLQHFITMFEYDYAKQVIWNT 92 Query: 61 FFIAVMKILGNLIVPVAFALLLNEVRHKRYKQSVQTLLYLPHFLSWVALAGVLVDILSPS 120 I+V+K++ LIVP+ ALLLNE+ K++VQT++YLP+FLSWV L G+L+D+LSP Sbjct: 93 LVISVLKMIFGLIVPIIVALLLNEMYQMVVKRTVQTIIYLPYFLSWVILGGILIDVLSPD 152 Query: 121 SGILNNVLGFFGVKPIFFLGNPDIFPYTLVITDVWKEFGWGTIIYLAAITTIDPTQYEAA 180 GI+N LG FG+ PIFFLG+ D F T+VI+D+WKE G+ TI++LAAIT ++P YEAA Sbjct: 153 GGIVNQALGLFGIDPIFFLGSNDWFRTTVVISDLWKETGFNTIVFLAAITAVNPNLYEAA 212 Query: 181 IIDGAGRWKQTMHVTLPALAPVIILLTVLGIGNVLQAGFDQVYNLYSPQVYSTGDIIDTL 240 ++DGA RWKQT+H+TLPA+ P+II++ L +GN+L AGFDQ++NLY+P VY TGDIIDT Sbjct: 213 VVDGANRWKQTLHITLPAMLPIIIVVATLSLGNILNAGFDQIFNLYNPLVYQTGDIIDTY 272 Query: 241 VYRIGIVDVQFGPAAAVGFFKSVVSFILIVTGYRLAHRWTGYRIF 285 VYR+ +V+ F AVG FKS++S +LI+ GYRLA+++ +RIF Sbjct: 273 VYRVSLVNGDFSYGTAVGLFKSLISLVLIIIGYRLAYKYANWRIF 317 >gb|AAC98126.1| integral membrane protein [Geobacillus stearothermophilus] Length = 360 Score = 293 bits (751), Expect = 2e-78, Method: Compositional matrix adjust. Identities = 146/270 (54%), Positives = 199/270 (73%), Gaps = 10/270 (3%) Query: 26 DYQTVFGFFDQE-WVGW---------DNFTYIFNLPGFYNVLWNTFFIAVMKILGNLIVP 75 D +T GF E W+G+ F +F V+WNT I+ +KI+ NL VP Sbjct: 91 DARTGHGFSSYEPWLGFFAFSLPLVSSIFKTMFEYEDARQVIWNTLVISTLKIIFNLGVP 150 Query: 76 VAFALLLNEVRHKRYKQSVQTLLYLPHFLSWVALAGVLVDILSPSSGILNNVLGFFGVKP 135 +AFALLLNEV ++K++VQT++YLPHFLSWV L G+L+D+LSP GI+N L FGVKP Sbjct: 151 LAFALLLNEVYVMKFKRTVQTIVYLPHFLSWVILGGILIDMLSPEGGIVNRFLSIFGVKP 210 Query: 136 IFFLGNPDIFPYTLVITDVWKEFGWGTIIYLAAITTIDPTQYEAAIIDGAGRWKQTMHVT 195 IFFLG+ D F +V+TDVWKE G+ TI++LAA+T+I + YEAAI+DGA RW+QT++++ Sbjct: 211 IFFLGDNDWFRTVVVVTDVWKECGFNTIVFLAALTSIKGSLYEAAIVDGANRWQQTIYIS 270 Query: 196 LPALAPVIILLTVLGIGNVLQAGFDQVYNLYSPQVYSTGDIIDTLVYRIGIVDVQFGPAA 255 LP++ P+II++ L +GN+L AGFDQ+ NLY+P VY TGDIIDT VYR+G+++ F A Sbjct: 271 LPSMLPIIIVVGTLSLGNILNAGFDQILNLYNPLVYKTGDIIDTYVYRVGLLNGDFSYAT 330 Query: 256 AVGFFKSVVSFILIVTGYRLAHRWTGYRIF 285 AVG FKSV+SFIL+VTGYRLA+++ Y+IF Sbjct: 331 AVGLFKSVISFILVVTGYRLAYKYANYKIF 360 >ref|NP_693011.1| hypothetical protein OB2090 [Oceanobacillus iheyensis HTE831] dbj|BAC14046.1| hypothetical conserved protein [Oceanobacillus iheyensis HTE831] Length = 320 Score = 239 bits (609), Expect = 7e-62, Method: Compositional matrix adjust. Identities = 119/279 (42%), Positives = 182/279 (65%) Query: 1 MLLPAVIITIIFRYVPIGGLSVAFMDYQTVFGFFDQEWVGWDNFTYIFNLPGFYNVLWNT 60 M+LP ++ I++Y+P+ GL ++F DY+ G EWVG+++F +F P F+ + NT Sbjct: 36 MILPGILYFFIYKYLPMTGLVISFQDYKPYLGISGSEWVGFEHFERLFTSPDFWMIFRNT 95 Query: 61 FFIAVMKILGNLIVPVAFALLLNEVRHKRYKQSVQTLLYLPHFLSWVALAGVLVDILSPS 120 + ++I+ +P+ +L+LNEVRH YK+ +QTL+Y+PHF+SWV + + +L+ Sbjct: 96 LILFALQIIIFFPIPIIISLMLNEVRHSLYKRGIQTLIYIPHFMSWVVIVSISYVMLTLD 155 Query: 121 SGILNNVLGFFGVKPIFFLGNPDIFPYTLVITDVWKEFGWGTIIYLAAITTIDPTQYEAA 180 GI+N+++ +FG + I FL N + F ++ +W+E GWGTII+LAAIT +DP YEAA Sbjct: 156 GGIINSLIEYFGFEKINFLMNEEWFRPMYILQVIWREAGWGTIIFLAAITGVDPQLYEAA 215 Query: 181 IIDGAGRWKQTMHVTLPALAPVIILLTVLGIGNVLQAGFDQVYNLYSPQVYSTGDIIDTL 240 +DGA R +Q H+TLPA+ VII++ +L IG+VL+ GF+ VY L + G+I DT Sbjct: 216 KMDGANRLRQMWHITLPAIRSVIIIMLILKIGDVLELGFEHVYLLLNASNREVGEIFDTY 275 Query: 241 VYRIGIVDVQFGPAAAVGFFKSVVSFILIVTGYRLAHRW 279 VY G+ QF + AVGFFK VV IL++ RLA R+ Sbjct: 276 VYTAGLQQGQFSYSTAVGFFKGVVGLILVIFANRLAKRF 314 >ref|NP_242978.1| transmembrane lipoprotein [Bacillus halodurans] pir||H83913 transmembrane lipoprotein BH2112 [imported] - Bacillus halodurans (strain C-125) dbj|BAB05831.1| transmembrane lipoprotein [Bacillus halodurans] Length = 337 Score = 228 bits (581), Expect = 1e-58, Method: Compositional matrix adjust. Identities = 124/287 (43%), Positives = 182/287 (63%), Gaps = 2/287 (0%) Query: 1 MLLPAVIITIIFRYVPIGGLSVAFMDYQTVFGFFDQEWVGWDNFTYIFNLPGFYNVLWNT 60 M LP +I IIFRYVP+ G S+AF D++ FDQ+W G+ +FT++F P F+ VL NT Sbjct: 51 MSLPFLIWLIIFRYVPLYGWSIAFQDFRPARPIFDQDWAGFKHFTFLFTDPRFFEVLRNT 110 Query: 61 FFIAVMKILGNLIVPVAFALLLNEVRHKRYKQSVQTLLYLPHFLSWVALAGVLVDILSPS 120 ++++ ++ + + A+LLNE+R+ +K+ VQT+ Y+PHFLSWV AG++ LS Sbjct: 111 LAMSLINLVLGFVTAIGLAILLNELRNLTFKRVVQTISYMPHFLSWVVAAGLVSAALSIE 170 Query: 121 SGILNNVLGFFGV--KPIFFLGNPDIFPYTLVITDVWKEFGWGTIIYLAAITTIDPTQYE 178 +GI+N VL G+ +PI +LG + F + +DVWK GW TIIYLAAIT ID QYE Sbjct: 171 NGIINIVLMKLGMISEPIMWLGVGEYFWGIIGASDVWKNVGWNTIIYLAAITMIDTEQYE 230 Query: 179 AAIIDGAGRWKQTMHVTLPALAPVIILLTVLGIGNVLQAGFDQVYNLYSPQVYSTGDIID 238 AA IDGA R ++ ++TLP + PVI++L ++ +G +L++GF+ Y L +P + +D Sbjct: 231 AAEIDGATRLQRIWYITLPGMRPVIVILLIMNLGYILESGFEAQYLLMNPMNMDFAENLD 290 Query: 239 TLVYRIGIVDVQFGPAAAVGFFKSVVSFILIVTGYRLAHRWTGYRIF 285 V R GI F A A G FK+VVSFI + + +A R R+F Sbjct: 291 IFVLRYGINMGNFSLATAAGIFKTVVSFIFLFSANHIAKRLGEARLF 337 >ref|ZP_00035516.1| COG4209: ABC-type polysaccharide transport system, permease component [Enterococcus faecium] Length = 324 Score = 225 bits (574), Expect = 8e-58, Method: Compositional matrix adjust. Identities = 115/278 (41%), Positives = 168/278 (60%) Query: 1 MLLPAVIITIIFRYVPIGGLSVAFMDYQTVFGFFDQEWVGWDNFTYIFNLPGFYNVLWNT 60 M+LP V+ +IFRY+P+GGL +AF DYQ G WVG+ +F +F P F+ +L NT Sbjct: 40 MILPGVLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNT 99 Query: 61 FFIAVMKILGNLIVPVAFALLLNEVRHKRYKQSVQTLLYLPHFLSWVALAGVLVDILSPS 120 + + I +P+ +LLLNEV +KR K +QT++Y+PHF+SWV + + L+ Sbjct: 100 LILFGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVD 159 Query: 121 SGILNNVLGFFGVKPIFFLGNPDIFPYTLVITDVWKEFGWGTIIYLAAITTIDPTQYEAA 180 G+LN L G + I FL +P+ + +WKE GW TIIYL+AIT +D YEAA Sbjct: 160 GGVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEAA 219 Query: 181 IIDGAGRWKQTMHVTLPALAPVIILLTVLGIGNVLQAGFDQVYNLYSPQVYSTGDIIDTL 240 +DGAGR ++T HVTLPA+ PVII L +L IG L GF+ +Y L + S +I DT Sbjct: 220 EMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIFDTY 279 Query: 241 VYRIGIVDVQFGPAAAVGFFKSVVSFILIVTGYRLAHR 278 +Y G+ + Q + A+G FK VV +++ ++A + Sbjct: 280 IYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKK 317 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.330 0.147 0.470 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 456,519,474 Number of extensions: 20008409 Number of successful extensions: 60627 Number of sequences better than 1.0e-05: 1073 Number of HSP's gapped: 59827 Number of HSP's successfully gapped: 1073 Length of query: 285 Length of database: 757,237,578 Length adjustment: 124 Effective length of query: 161 Effective length of database: 420,565,302 Effective search space: 67711013622 Effective search space used: 67711013622 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 125 (52.8 bits)