BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0569.0005.p (275 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_00086953.1| COG4221: Short-chain alcohol dehydrogenase of... 138 1e-31 ref|ZP_00033073.1| COG4221: Short-chain alcohol dehydrogenase of... 134 3e-30 gb|EAI46203.1| unknown [environmental sequence] 133 4e-30 ref|ZP_00111652.1| COG4221: Short-chain alcohol dehydrogenase of... 132 9e-30 ref|NP_435419.1| putative [Sinorhizobium meliloti 1021] >gi|2528... 130 4e-29 >ref|ZP_00086953.1| COG4221: Short-chain alcohol dehydrogenase of unknown specificity [Pseudomonas fluorescens PfO-1] Length = 252 Score = 138 bits (348), Expect = 1e-31, Method: Compositional matrix adjust. Identities = 88/263 (33%), Positives = 137/263 (52%), Gaps = 31/263 (11%) Query: 2 LNHKTAVITGASSGIGRAIATRLAAQGVHVFITGRSREQLEEVKSQIELAGGQATCSAFD 61 + +K +ITGASSGIG A A +AA+G HV + R E+L+++ S+I+ GG A+ A D Sbjct: 12 IQNKVVLITGASSGIGEAAARLIAAKGAHVVLGARRTERLDKLVSEIQAEGGSASACALD 71 Query: 62 LRDTGRLQDFIRQTRTQTGHLDIMVNNAGLHIHGNIVDTKVEDWREMIDVNIVALLAGSQ 121 + D + F+ +TQ G +D+++NNAG+ + KV++W +M+DVN+ +L G Sbjct: 72 VTDLQSMHAFVAFAKTQHGKVDVIINNAGVMPLSPLASLKVDEWNQMLDVNVRGVLHGIA 131 Query: 122 AAVQAMRLNEGGAPGHIINISS--GNAYEPGRDFYSATKHMVNAIGQALTRELAQEPIQI 179 A + M + G +INISS G A P Y ATK V AI L +E + I++ Sbjct: 132 AVLPDM---QAQGFGQVINISSIGGLAVSPTAAVYCATKFAVRAISDGLRQE--TDKIRV 186 Query: 180 TTILPGAVXXXXGRNLEPEAVVQLARKLGMEVEYKQGDHLPEELIARISGAMGQTFLSGD 239 T + PG VE + D + +E+ A + L+ D Sbjct: 187 TVVCPGV------------------------VESELADTISDEVAREEMKAFRRVALNAD 222 Query: 240 DIARAVVYALQQPQNVVVRELYL 262 IARA+ YA++QP +V V E+ + Sbjct: 223 AIARALTYAIEQPDDVDVSEIVV 245 >ref|ZP_00033073.1| COG4221: Short-chain alcohol dehydrogenase of unknown specificity [Burkholderia fungorum] Length = 246 Score = 134 bits (336), Expect = 3e-30, Method: Compositional matrix adjust. Identities = 100/265 (37%), Positives = 135/265 (50%), Gaps = 35/265 (13%) Query: 2 LNHKTAVITGASSGIGRAIATRLAAQGVHVFITGRSREQLEEVKSQIELAGG--QATCSA 59 + K +ITGASSGIG A A RLA QG + + R E+L + +Q+ GG + A Sbjct: 6 IEDKVVMITGASSGIGEATARRLAQQGAKLVLAARRSERLSRLAAQL---GGADRVLWDA 62 Query: 60 FDLRDTGRLQDFIRQTRTQTGHLDIMVNNAGLHIHGNIVDTKVEDWREMIDVNIVALLAG 119 D+ LQ R + GHLD++VNNAG+ I +V+DW MIDVNI +L G Sbjct: 63 TDVTKPEALQQLAATARERFGHLDVLVNNAGIMPVSLIAQGRVDDWNRMIDVNIKGVLYG 122 Query: 120 SQAAVQAMRLNEGGAPGHIINISS--GNAYEPGRDFYSATKHMVNAIGQALTRELAQEPI 177 A + AM L G GHIINISS G + PG YSATK V AI + L +E A + + Sbjct: 123 IHAVLGAM-LERG--VGHIINISSVAGLSVGPGGAVYSATKFAVRAISEGLRQECAGK-V 178 Query: 178 QITTILPGAVXXXXGRNLEPEAVVQLARKLGMEVEYKQGDHLPEELIARISGAMGQTFLS 237 ++T+I PG V ++ A + A+KL GAM Sbjct: 179 RVTSICPGLVASELPDSITVPAFRERAQKL-------------------FEGAM-----P 214 Query: 238 GDDIARAVVYALQQPQNVVVRELYL 262 D +A A+VYAL QP+ V V E+ + Sbjct: 215 ADAVADAIVYALTQPEQVAVNEIVV 239 >gb|EAI46203.1| unknown [environmental sequence] Length = 247 Score = 133 bits (335), Expect = 4e-30, Method: Compositional matrix adjust. Identities = 90/264 (34%), Positives = 130/264 (49%), Gaps = 31/264 (11%) Query: 2 LNHKTAVITGASSGIGRAIATRLAAQGVHVFITGRSREQLEEVKSQIELAGGQATCSAFD 61 ++ KT VITGASSG+G A A LA+ G V + R ++L+++ + IE GGQA D Sbjct: 5 ISGKTVVITGASSGLGEATAYHLASLGARVVLGARREDKLQQIAAHIEAKGGQAAYLVTD 64 Query: 62 LRDTGRLQDFIRQTRTQTGHLDIMVNNAGLHIHGNIVDTKVEDWREMIDVNIVALLAGSQ 121 + +Q ++ ++ G LD+++NNAGL I + K ++W MID+NI +L G Sbjct: 65 VTKASEVQALVQLAVSRFGQLDVIINNAGLMSIAPITELKTDEWDRMIDINIKGVLYGIA 124 Query: 122 AAVQAMRLNEGGAPGHIINISSG---NAYEPGRDFYSATKHMVNAIGQALTRELAQEPIQ 178 AA+ + GH INISS + PG YS TK V AI + L E+ I+ Sbjct: 125 AALPVFEQQQS---GHFINISSVAGIKVFSPGGTVYSGTKFAVRAISEGLRHEVGG-AIR 180 Query: 179 ITTILPGAVXXXXGRNLEPEAVVQLARKLGMEVEYKQGDHLPEELIARISGAMGQTFLSG 238 TTILPGAV + E K G + A Q + Sbjct: 181 TTTILPGAV----------------------DSELKHGSSHQQS--AEFVNNFYQIAIPA 216 Query: 239 DDIARAVVYALQQPQNVVVRELYL 262 D +A+AV YA+ QP+ V + E+ L Sbjct: 217 DSVAKAVAYAIAQPKEVDINEIVL 240 >ref|ZP_00111652.1| COG4221: Short-chain alcohol dehydrogenase of unknown specificity [Nostoc punctiforme] Length = 249 Score = 132 bits (332), Expect = 9e-30, Method: Compositional matrix adjust. Identities = 85/263 (32%), Positives = 139/263 (52%), Gaps = 27/263 (10%) Query: 2 LNHKTAVITGASSGIGRAIATRLAAQGVHVFITGRSREQLEEVKSQIELAGGQATCSAFD 61 L + A+ITGAS+GIG A A LAA+G V + R ++++ + +IE +GG+A D Sbjct: 5 LKEQVAIITGASAGIGEATAIALAAEGATVVLAARRGDRIQALAERIEASGGKALPIVTD 64 Query: 62 LRDTGRLQDFIRQTRTQTGHLDIMVNNAGLHIHGNIVDTKVEDWREMIDVNIVALLAGSQ 121 + D ++ + + + G +DI+VNNAG+ + G I DWR D+N++ LL + Sbjct: 65 VTDENQVNHLVAKANVELGRVDILVNNAGIALLGTIEAGNSSDWRRSFDINVLGLLYATH 124 Query: 122 AAVQAMRLNEGGAPGHIINISS--GNAYEPGRDFYSATKHMVNAIGQALTRELAQEPIQI 179 A + ++ + GHI+NISS G G Y+ATK VNA+ +AL +E+ ++ I++ Sbjct: 125 AVLPLLKAQKS---GHIVNISSVAGRTARAGVGVYNATKWGVNALSEALRQEVHKDNIRV 181 Query: 180 TTILPGAVXXXXGRNLEPEAVVQLARKLGMEVEYKQGDHLPEELIARISGAMGQTFLSGD 239 T I PG V E++ + D + ++ I A+ T L + Sbjct: 182 TIIEPGLVD--------------------TEIDNQITDPVAKQRIEERRKAI--TPLHSE 219 Query: 240 DIARAVVYALQQPQNVVVRELYL 262 D+A A+VYA+ QP V V E+ + Sbjct: 220 DVAAAIVYAVTQPSRVNVNEILI 242 >ref|NP_435419.1| putative [Sinorhizobium meliloti 1021] pir||E95283 probable [imported] - Sinorhizobium meliloti (strain 1021) magaplasmid pSymA gb|AAK64831.1| putative [Sinorhizobium meliloti] Length = 249 Score = 130 bits (326), Expect = 4e-29, Method: Compositional matrix adjust. Identities = 94/264 (35%), Positives = 135/264 (51%), Gaps = 28/264 (10%) Query: 2 LNHKTAVITGASSGIGRAIATRLAAQGVHVFITGRSREQLEEVKSQIELAGGQATCSAFD 61 LN K A++TGASSGIG A A +LA G V I R ++LE++K +IE GG+A D Sbjct: 4 LNGKIALVTGASSGIGAATAAKLAEAGAKVGIAARRTDKLEDLKKKIEAKGGEALVIEMD 63 Query: 62 LRDTGRLQDFIRQTRTQTGHLDIMVNNAGLHIHGNIVDTKVEDWREMIDVNIVALLAGSQ 121 + DT ++ +++ G +DI+VNNAGL +I KV++W+ M+DVN+ LL + Sbjct: 64 VVDTTSVEAGVKKLVDAYGSIDILVNNAGLMPLSDIDQFKVDEWQRMVDVNVKGLLNTTA 123 Query: 122 AAVQAMRLNEGGAPGHIINISS--GNAYEPGRDFYSATKHMVNAIGQALTRELAQE-PIQ 178 A + M GH+ N+SS G G Y ATKH V A L E+ Q+ I+ Sbjct: 124 AVLPQMIKQH---SGHVFNMSSIAGRKVFKGLSVYCATKHAVTAFSDGLRMEVGQKHGIR 180 Query: 179 ITTILPGAVXXXXGRNLEPEAVVQLARKLGMEVEYKQGDHLPEELIARISGAMGQTFLSG 238 +T I PGAV ++ Q Q D L ++ TFL G Sbjct: 181 VTCIQPGAVATELYDHITDPGYRQ------------QMDELATQM----------TFLQG 218 Query: 239 DDIARAVVYALQQPQNVVVRELYL 262 +DI +V+A Q P +V V EL++ Sbjct: 219 EDIGDTIVFAAQAPAHVDVAELFV 242 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.318 0.134 0.376 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 375,317,874 Number of extensions: 14163556 Number of successful extensions: 63705 Number of sequences better than 1.0e-05: 7022 Number of HSP's gapped: 57432 Number of HSP's successfully gapped: 7030 Length of query: 275 Length of database: 757,237,578 Length adjustment: 124 Effective length of query: 151 Effective length of database: 420,565,302 Effective search space: 63505360602 Effective search space used: 63505360602 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 125 (52.8 bits)