BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0569.0001.p (327 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_786293.1| metal-dependent hydrolase (putative) [Lactobaci... 263 3e-69 gb|EAA73433.1| hypothetical protein FG03965.1 [Gibberella zeae P... 201 2e-50 gb|EAH90682.1| unknown [environmental sequence] 192 8e-48 ref|ZP_00027461.1| COG2159: Predicted metal-dependent hydrolase ... 169 9e-41 ref|NP_769383.1| bll2743 [Bradyrhizobium japonicum] >gi|27351000... 158 1e-37 >ref|NP_786293.1| metal-dependent hydrolase (putative) [Lactobacillus plantarum WCFS1] emb|CAD65149.1| metal-dependent hydrolase (putative) [Lactobacillus plantarum WCFS1] Length = 307 Score = 263 bits (673), Expect = 3e-69, Method: Compositional matrix adjust. Identities = 140/314 (44%), Positives = 200/314 (63%), Gaps = 10/314 (3%) Query: 11 NKNDLHSHYLPSAYREALLKHAGPNPDGFPTPVWHADLHLEAMEQLGIAVSLLSLSSPHI 70 +K D H+HYLP++Y EAL +H +PDG+PTP W L L M I+ S+LSLSSPH+ Sbjct: 2 SKIDFHTHYLPTSYVEALKRHVPGDPDGWPTPEWTPQLTLNFMRDNDISYSILSLSSPHV 61 Query: 71 HWGDREAARELARRVNEDGAELVRKYPGKFGLLASLPVPEISDSIVEIKYAMDVLHADGF 130 ++GD+ L N+DG L ++YP + G LASLP+P D++ ++ A+D A G Sbjct: 62 NFGDKAETIRLVEAANDDGKSLAQQYPDQLGYLASLPIPYELDAVKTVQQALDQDGALGV 121 Query: 131 TLPTHALGVYLGNPCLHDIFAELNTRKAIVALHPNRPALVPEHVLERLPAPMMEFFFDTT 190 T+PT++ G+Y G+P L ++ EL+ R+AIVALHPN PA++P++V LP P++ FF DTT Sbjct: 122 TVPTNSRGLYFGSPVLERVYQELDARQAIVALHPNEPAILPKNVDIDLPVPLLGFFMDTT 181 Query: 191 RTVMNLIFTGTFSRFPDITFIIPHAGAFLPVLADRIAPALHMMPSIFGEHTEDAGVDIYT 250 T +N++ F ++P+I IIPHAGAFL ++ DRIA + VD+Y Sbjct: 182 MTFINMLKYHFFEKYPNIKVIIPHAGAFLGIVDDRIAQYAQKV----------YQVDVYD 231 Query: 251 SLKGLYYDIAGACLPRQLDNLLQLIGTDHLLYGSDYPYTPADACKSLADGLEGTNLLTDE 310 + +Y+D+AGA LPRQL L+ L +HLLYGSD PYTP D + L L T+LLT+E Sbjct: 232 VMHHVYFDVAGAVLPRQLPTLMSLAQPEHLLYGSDIPYTPLDGSRQLGHALATTDLLTNE 291 Query: 311 ARCQIYGENALRLF 324 + I+ +NA RL Sbjct: 292 QKQAIFYDNAHRLL 305 >gb|EAA73433.1| hypothetical protein FG03965.1 [Gibberella zeae PH-1] Length = 336 Score = 201 bits (511), Expect = 2e-50, Method: Compositional matrix adjust. Identities = 118/313 (37%), Positives = 178/313 (56%), Gaps = 18/313 (5%) Query: 14 DLHSHYLPSAYREALLKHAGPNPDGFPT-PVWHADLHLEAMEQLGIAVSLLSLSSPHIHW 72 DLH H + +YR+ + + PDG P P W + H+ M++L I+ S+LS++SP H Sbjct: 6 DLHCHAVAPSYRQYAIDNGHEKPDGMPALPQWTPEQHIALMKKLNISKSVLSITSPGTHL 65 Query: 73 G--DREAARELARRVNEDGAELVRKYPGKFGLLASLPVPEISDSIVEIKYAMDVLHADGF 130 + E A L R+VN++ + +++P F ASLP+P +++SI EI YA+D L A GF Sbjct: 66 TPQNDENATLLTRQVNKELFTICKEHPSYFSFFASLPLPSVNNSIAEIDYALDQLGALGF 125 Query: 131 TLPTHALGVYLGNPCLHDIFAELNTRKAIVALHPNRPALV-------PEHVLERLPAPMM 183 + ++A GVYLG+ L +FA LN RKAI+ +HP ++ P LE+ P PMM Sbjct: 126 AVLSNANGVYLGDAELDPVFAHLNARKAILFIHPTTCNILASAGQVQPVKPLEKYPRPMM 185 Query: 184 EFFFDTTRTVMNLIFTGTFSRFPDITFIIPHAGAFLPVLADRIAPALHMMPSIFGEHTED 243 EF FD TR + NL+ +GT +++P I F++ H G LP + DRI + I G ++ Sbjct: 186 EFMFDETRAIANLLLSGTVAKYPGIKFMMSHCGCALPSMLDRIGAFATL---ISGAESQT 242 Query: 244 AGVDIYTSLKGLYYDIAGACLPRQLDNLLQLIG---TDHLLYGSDYPYTPADACKSLADG 300 A + Y+D+AG P + LL+++G L+YG+DYP+TP SLAD Sbjct: 243 AEFQKLLRER-FYFDLAGFPFPNAIHGLLRILGEGAEKRLVYGTDYPFTPERLVVSLADV 301 Query: 301 LE-GTNLLTDEAR 312 +E G L DE + Sbjct: 302 MEKGLEELFDEGQ 314 >gb|EAH90682.1| unknown [environmental sequence] Length = 323 Score = 192 bits (488), Expect = 8e-48, Method: Compositional matrix adjust. Identities = 114/318 (35%), Positives = 164/318 (51%), Gaps = 14/318 (4%) Query: 10 ANKNDLHSHYLPSAYREALLKHAGPNPDGFPTPVWHADLHLEAMEQLGIAVSLLSLSSPH 69 A K D H H +P Y A L G G P W + L+ ++ GIA + S+S+P Sbjct: 2 AGKIDTHHHIVPPTY-AAWLTQRGITAGGLAIPSWSEEAALDLIDASGIATGIFSVSTPG 60 Query: 70 IHWGDREAARELARRVNEDGAELVRKYPGKFGLLASLPVPEISDSIVEIKYAMDVLHADG 129 +H GD ARE AR VNE A +V YPG+FG A+L +P++ +I E K+A D L+ADG Sbjct: 61 VHLGDDVEAREKAREVNEFAANIVAGYPGRFGFFATLTLPDVEGAITEAKHAFDQLNADG 120 Query: 130 FTLPTHALGVYLGNPCLHDIFAELNTRKAIVALHPNRPALVPEHVLERLPAPMMEFFFDT 189 L + G YLG+ +F ELN R A+V +HP +P + +P +F DT Sbjct: 121 VVLISSVRGTYLGDESWAPLFDELNRRHAVVFVHPGD---LPAEPVPGIPPYAADFLLDT 177 Query: 190 TRTVMNLIFTGTFSRFPDITFIIPHAGAFLPVLADRIAPALHMMPSIFGEHTEDAGVDIY 249 TR +N+ +G RFP++ I+ HAG F+P A RIA + + + D G+ Sbjct: 178 TRAAINVAKSGWLERFPNVRIILSHAGGFIPYAAQRIARVVSP------DGSRDGGL--- 228 Query: 250 TSLKGLYYDIAGACLPRQLDNLLQLIGTDHLLYGSDYPYTPADACKSLADGLEGTNLLTD 309 L+ Y+D A + P + LL H+ +GSD+PY P + + + L L D Sbjct: 229 ARLRRFYFDTALSSSPYAMPALLAFADPTHITFGSDWPYAPKSSSLAFTEMLHAFPLTPD 288 Query: 310 EARCQIYGENALRLFPGL 327 + R IY NA +LFP L Sbjct: 289 Q-RHAIYRGNAEQLFPRL 305 >ref|ZP_00027461.1| COG2159: Predicted metal-dependent hydrolase of the TIM-barrel fold [Burkholderia fungorum] Length = 291 Score = 169 bits (427), Expect = 9e-41, Method: Compositional matrix adjust. Identities = 101/287 (35%), Positives = 156/287 (54%), Gaps = 9/287 (3%) Query: 42 PVWHADLHLEAMEQLGIAVSLLSLSSPHIHWGDREAARELARRVNEDGAELVRKYPGKFG 101 P W D L M++ GI ++ S+++P + A LAR NE A +VR +PG+FG Sbjct: 2 PQWSVDEALSLMDRSGIGTAITSITAPGVSALAEVEAGGLARWCNEFSANMVRDWPGRFG 61 Query: 102 LLASLPVPEISDSIVEIKYAMDVLHADGFTLPTHALGVYLGNPCLHDIFAELNTRKAIVA 161 + A LP+ I +++ E Y+++ L ADG L ++ G YLG+P + ++ ELN RKA+V Sbjct: 62 MFACLPLQSIDNALREAVYSLETLKADGVCLLSNYDGRYLGDPVMRPLYEELNRRKAVVF 121 Query: 162 LHPNRPA-LVPEHVLERLPAPMMEFFFDTTRTVMNLIFTGTFSRFPDITFIIPHAGAFLP 220 +HP PA +VP +++L +EF FDTTRTV +LIF G + +P+I +I+ HAG +P Sbjct: 122 VHPTSPASMVP---IDQLSPSSLEFPFDTTRTVASLIFGGITTDYPEIRWILSHAGGAMP 178 Query: 221 VLADRIAPALHMMPSIFGEHTEDAGVDIYTSLKGLYYDIAGACLPRQLDNLLQLIGTDHL 280 LA RI P++ E D ++ ++D A + P Q L +L+ L Sbjct: 179 YLAGRIELLTRNNPAL-RERIPDG---FRRAMATFFFDCALSVDPAQFAALRELVPDSQL 234 Query: 281 LYGSDYPYTPADACKSLADGLEGTNLLTDEARCQIYGENALRLFPGL 327 L+G+DYP+ P G+ L + + +NAL LFP L Sbjct: 235 LFGTDYPFGPKGQIAETVSGVLDVGLEPGTLKA-LESDNALSLFPRL 280 >ref|NP_769383.1| bll2743 [Bradyrhizobium japonicum] dbj|BAC48008.1| bll2743 [Bradyrhizobium japonicum USDA 110] Length = 364 Score = 158 bits (400), Expect = 1e-37, Method: Compositional matrix adjust. Identities = 114/321 (35%), Positives = 166/321 (51%), Gaps = 20/321 (6%) Query: 14 DLHSHYLPSAYREALLKH--AGPNPDGFPTPV-WHADLHLEAMEQLGIAVSLLSLSSPHI 70 D H H+ P Y++ L + A P FP V W + +E M++ GI +LS++S Sbjct: 55 DTHLHFYPPEYQKLWLGYEDARKQPH-FPGQVAWTREKLVEDMDRNGIRTGILSVASTPG 113 Query: 71 HW---GDREAARELARRVNEDGAELVRKYPGKFGLLASLPVPEISDSIVEIKYAMDVLHA 127 W G EA R LAR NE A+++R +PG+FGL A+L + ++ ++ EI+YA+D L A Sbjct: 114 VWFDLGPAEAGR-LARTCNEYAADMMRDHPGRFGLFATLSMLDVDATLKEIEYALDTLKA 172 Query: 128 DGFTLPTHALGVYLGNPCLHDIFAELNTRKAIVALHPNRPALVPEHVLERLPAPMMEFFF 187 DG L + +LGN +F ELN RKA+V +HP + + PA ++E Sbjct: 173 DGIGLQSSYGDKWLGNAAYRPVFEELNRRKAVVYVHPLVASCCSALSVGTFPA-VIEVPH 231 Query: 188 DTTRTVMNLIFTGTFSRFPDITFIIPHAGAFLPVLADRIAPALHMMPSIFGEHTEDAGVD 247 DTTRTV +L+ +G F+R DI ++ HAG +P++A RI P + E A Sbjct: 232 DTTRTVTSLLLSGAFARHRDIKWLFSHAGGTIPMMAGRINSFYGARPDL----KEFAPEG 287 Query: 248 IYTSLKGLYYDIAGACLPRQLDNLLQLIGTDHLLYGSDYPYTPADACKSLAD-GLEGTNL 306 I L+ L+YD A A + LL+L+ + YG+DYPY L GL +L Sbjct: 288 IEGELRRLHYDTANATFAPSMAALLKLVPASQITYGTDYPYFGLGQFAQLQKLGLSTEDL 347 Query: 307 LTDEARCQIYGENALRLFPGL 327 I ENA+RL P L Sbjct: 348 DA------IGHENAIRLVPRL 362 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.322 0.140 0.432 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 523,542,500 Number of extensions: 23233304 Number of successful extensions: 46131 Number of sequences better than 1.0e-05: 80 Number of HSP's gapped: 46022 Number of HSP's successfully gapped: 80 Length of query: 327 Length of database: 757,237,578 Length adjustment: 126 Effective length of query: 201 Effective length of database: 415,135,104 Effective search space: 83442155904 Effective search space used: 83442155904 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 126 (53.1 bits)