BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0566.0029.p (339 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_828614.1| putative oxidoreductase [Streptomyces avermitil... 309 6e-83 gb|EAJ13757.1| unknown [environmental sequence] 282 1e-74 ref|NP_268407.1| oxidoreductase [Lactococcus lactis subsp. lacti... 280 4e-74 gb|AAS07910.1| oxidoreductase, short-chain dehydrogenase/reducta... 264 2e-69 gb|EAI91407.1| unknown [environmental sequence] 258 1e-67 >ref|NP_828614.1| putative oxidoreductase [Streptomyces avermitilis MA-4680] dbj|BAC75149.1| putative oxidoreductase [Streptomyces avermitilis MA-4680] Length = 322 Score = 309 bits (791), Expect = 6e-83, Method: Compositional matrix adjust. Identities = 173/327 (52%), Positives = 218/327 (66%), Gaps = 13/327 (3%) Query: 10 TVQAPLASGFDARTTAAEVIGGLDLTGKVAIVTGGYSGIGLETSRVLAEAGATVIVPART 69 T Q + SGF A +TA +V+ G+DL+G++AIVTGGYSG+GLET+R L AGA V+VPAR Sbjct: 3 TAQHKIGSGFGATSTAVDVLHGIDLSGQLAIVTGGYSGLGLETTRALTGAGARVVVPARR 62 Query: 70 PAKAKAAVSGIPRLELETLDLMDPASIDSFARRFIASGRPLHFLINSAGIMAAPLARDAR 129 P A+ AV+GI +E++ LDL D S+ FA RF+ASGR + +IN+AGIMA P R Sbjct: 63 PDVAREAVAGIDGVEVDELDLGDLESVRGFAERFLASGRTIDIVINNAGIMACPETRVGP 122 Query: 130 GYESQFATNHLGHFQLTARLWPALRQADQARVISVSSRAHRLGGVDLSDPNFERREYDKW 189 G+E+QFATNHLGHF L RLWPA+ + ARV+SVSS H L G+ D ++ +YDKW Sbjct: 123 GWEAQFATNHLGHFALVNRLWPAV-EPGGARVVSVSSGGHHLSGIRWDDVHWT-HDYDKW 180 Query: 190 KSYAQSKSANVLFAVALDEKAKVYGVRAFAVHPGLIPSSGIGRFLKTEEVGVKPVKVQPA 249 ++Y Q+K+ANVLFAV LD + GVRAF++HPG I + + R L EE+ V+ Sbjct: 181 EAYGQAKTANVLFAVHLDRLGRESGVRAFSLHPGGILTP-LQRHLAKEEM------VERG 233 Query: 250 QEDEKGKPASNTNSDFMKNTEQGAATSVWCAVSEQLAGLGGVYCEDADIAEAVPADSVQG 309 DE G + D K EQGAAT VW A S QLAG+GGVYCED DIAE AD +G Sbjct: 234 WIDENGDL---VHPDAFKTPEQGAATQVWAATSPQLAGMGGVYCEDCDIAEPASADGERG 290 Query: 310 SGVWPWAIDREAAERLWVLSEQLTGVK 336 GV WA D E A RLW LS +LTGV Sbjct: 291 -GVKDWATDPEQAARLWDLSAELTGVN 316 >gb|EAJ13757.1| unknown [environmental sequence] Length = 321 Score = 282 bits (721), Expect = 1e-74, Method: Compositional matrix adjust. Identities = 162/324 (50%), Positives = 205/324 (63%), Gaps = 14/324 (4%) Query: 12 QAPLASGFDARTTAAEVIGGLDLTGKVAIVTGGYSGIGLETSRVLAEAGATVIVPARTPA 71 Q PL SGF A +TA++V+ LDL GK AIVTGG+SG+GLET+R LAEAGATVIV AR+ Sbjct: 5 QHPLHSGFGAASTASDVLADLDLHGKTAIVTGGHSGLGLETTRALAEAGATVIVGARSVD 64 Query: 72 KAKAAVSGIPRLELETLDLMDPASIDSFARRFIASGRPLHFLINSAGIMAAPLARDARGY 131 A+ A G+ +E+ LDL D AS+ +FA RF+ + R + +INSAG+MA P R G Sbjct: 65 AAREATRGVAGVEVAALDLADLASVAAFAARFVDAHRDVDIVINSAGVMACPETRVGDGR 124 Query: 132 ESQFATNHLGHFQLTARLWPALRQADQARVISVSSRAHRLGGVDLSDPNFERREYDKWKS 191 E+Q A NHLGH+ L LWP L Q D ARV++VSS HRL + D F YDKW + Sbjct: 125 EAQMAVNHLGHYALVNGLWPVLAQGDGARVVAVSSLGHRLSPIRWDDVQFA-HGYDKWLA 183 Query: 192 YAQSKSANVLFAVALDEKAKVYGVRAFAVHPGLIPSSGIGRFLKTEEVGVKPVKVQPAQE 251 Y QSK+AN LFAV LD +GVRA+++HPG I ++ + R L EE+ + Sbjct: 184 YGQSKTANALFAVQLDALGAPFGVRAYSLHPGKI-ATPLQRHLTREEM------MAQGWV 236 Query: 252 DEKGKPASNTNSDFMKNTEQGAATSVWCAVSEQLAGLGGVYCEDADIAEAVPADSVQGSG 311 DE G P T K QGAAT VW A S ++AG+GGVYCED DI AV + + SG Sbjct: 237 DEHGVPIDPT----FKTPAQGAATQVWAATSPRVAGIGGVYCEDCDI--AVVTEGNEESG 290 Query: 312 VWPWAIDREAAERLWVLSEQLTGV 335 V P AID E A RLW S ++TG+ Sbjct: 291 VRPHAIDPEQAVRLWAWSAEVTGI 314 >ref|NP_268407.1| oxidoreductase [Lactococcus lactis subsp. lactis] pir||B86906 oxidoreductase yxdE [imported] - Lactococcus lactis subsp. lactis (strain IL1403) gb|AAK06348.1| oxidoreductase [Lactococcus lactis subsp. lactis] Length = 339 Score = 280 bits (716), Expect = 4e-74, Method: Compositional matrix adjust. Identities = 149/340 (43%), Positives = 213/340 (62%), Gaps = 13/340 (3%) Query: 9 LTVQAPLAS-GFDARTTAAEVIGGLDLTGKVAIVTGGYSGIGLETSRVLAEAGATVIVPA 67 ++ Q P+A+ F+ +TTA +V+ ++L+GK I+TGGYSGIGLE ++ L+ AGA +I+PA Sbjct: 1 MSNQEPIANYKFNYKTTANKVMKNINLSGKTIIITGGYSGIGLEMTKQLSRAGAKIIIPA 60 Query: 68 RTPAKAKAAVSGIPRLELETLDLMDPASIDSFARRFIASGRPLHFLINSAGIMAAPLARD 127 R AK +S I +++ LDL +P SI FA F+ + L LINSAG+M PL RD Sbjct: 61 RRMEVAKKNLSKIKNIDILPLDLTNPESIHQFAESFLQNNSKLDILINSAGLMYLPLKRD 120 Query: 128 ARGYESQFATNHLGHFQLTARLWPALRQADQARVISVSSRAHRLGGVDLSDPNFERRE-Y 186 +RG E FATNHLGH+QLT L PAL+ A +RVI++SSRA ++ + L D NFE+ E Y Sbjct: 121 SRGIEYHFATNHLGHYQLTLELLPALKNAKISRVINLSSRAQQMTPL-LEDWNFEKIENY 179 Query: 187 DKWKSYAQSKSANVLFAVALDEKAKVYGVRAFAVHPGLIPSSGIGR-------FLKTEEV 239 + Y QSK+ANVLF++ LDE K YG+R+FAVHPG+IP + IGR +L+ Sbjct: 180 NPQIGYQQSKTANVLFSIKLDELGKKYGIRSFAVHPGMIPMTNIGRQNSPITPWLRYLAD 239 Query: 240 GVKPVKVQPAQEDEKGKPASNTNSDFMKNTEQGAATSVWCAVSEQLAGLGGVYCEDADIA 299 K + + P + + ++K QGAAT +W + S L G GG+YCED +IA Sbjct: 240 YFKLIHI-PVFFNALKSGFDRSKYRYLKTIAQGAATPLWASTSPDLEGKGGLYCEDLNIA 298 Query: 300 EAVPADSVQG--SGVWPWAIDREAAERLWVLSEQLTGVKF 337 + ++ G+ P AID A+RLW +S+ +TG+ F Sbjct: 299 RLMTSEEANNFSGGLRPHAIDHNDADRLWQISKNITGLDF 338 >gb|AAS07910.1| oxidoreductase, short-chain dehydrogenase/reductase family [uncultured bacterium 463] Length = 314 Score = 264 bits (675), Expect = 2e-69, Method: Compositional matrix adjust. Identities = 146/329 (44%), Positives = 200/329 (60%), Gaps = 22/329 (6%) Query: 17 SGFDARTTAAEVIGGLDLTGKVAIVTGGYSGIGLETSRVLAEAGATVIVPARTPAKAKAA 76 S F + T+ +EVI G+DL GK A+VTG SG+G+ETSR LA AGA VI+ AR +K A Sbjct: 2 SDFGSGTSTSEVIEGVDLKGKCALVTGASSGLGVETSRSLASAGAAVIMVARDASKLDTA 61 Query: 77 VS----GIPRLELET--LDLMDPASIDSFARRFIASGRPLHFLINSAGIMAAPLARDARG 130 V+ +P +L+T LDL D S+ + A+ +A + LIN+AG+MA PL R A+G Sbjct: 62 VAQVRAAVPDAQLDTALLDLADLESVRAGAQTILAKCPSIQLLINNAGVMACPLMRTAQG 121 Query: 131 YESQFATNHLGHFQLTARLWPALRQADQARVISVSSRAHRLGGVDLSDPNFERREYDKWK 190 +E Q TNH+GHF LT L PAL ARV+++SS HR +DL DPN+ RR+Y+KW+ Sbjct: 122 FEMQLGTNHVGHFLLTCMLAPALVAGAPARVVNLSSAGHRFSAMDLDDPNYHRRDYEKWQ 181 Query: 191 SYAQSKSANVLFAVALDEKAKVYGVRAFAVHPGLIPSSGIGRFLKTEEVGVKPVKVQPAQ 250 +Y QSK+AN LF+V LD++ + GVR+FAVHPG+I + + R + ++ Sbjct: 182 AYGQSKTANALFSVGLDQRLQGQGVRSFAVHPGMIMTE-LSRHMDPSDM----------- 229 Query: 251 EDEKGKPASNTNSDFMKNTEQGAATSVWCAVSEQLAGLGGVYCEDADIAEAVPADSVQGS 310 E N K EQG+ATSVW A S+ L GLGG+Y ED IAE PA + Sbjct: 230 --EIILAGRNIEDIGFKTVEQGSATSVWAATSQDLDGLGGLYLEDCHIAE--PATPDNEA 285 Query: 311 GVWPWAIDREAAERLWVLSEQLTGVKFTI 339 G+ +A+D A++LW LSE + G F Sbjct: 286 GIESYALDPVTADKLWQLSEDMVGENFNF 314 >gb|EAI91407.1| unknown [environmental sequence] Length = 325 Score = 258 bits (659), Expect = 1e-67, Method: Compositional matrix adjust. Identities = 144/327 (44%), Positives = 196/327 (59%), Gaps = 12/327 (3%) Query: 10 TVQAPLASGFDARTTAAEVIGGLDLTGKVAIVTGGYSGIGLETSRVLAEAGATVIVPART 69 +Q P+ SGFD+++ A +++ DL GK+AIVTGGYSGIGLETSR L GA VI+PA+ Sbjct: 4 NLQKPIISGFDSKSDADKIVKEKDLGGKIAIVTGGYSGIGLETSRALVAIGAKVIIPAKR 63 Query: 70 PAKAKAAVSGIPRLE-LETLDLMDPASIDSFARRFIASGRPLHFLINSAGIMAAPLARDA 128 A + GI E + +DL + S+ F + F S L LIN+AGIMA P R Sbjct: 64 TEVAVQNLEGIVSKENIVEMDLGNLNSVRKFTKDFKESFGKLDLLINNAGIMACPETRIG 123 Query: 129 RGYESQFATNHLGHFQLTARLWPALRQADQARVISVSSRAHRLGGVDLSDPNFERREYDK 188 G+ESQFA NH+GHF LT L + + D AR +S+SS AH L G+ D +F+ YDK Sbjct: 124 NGWESQFAVNHIGHFLLTKELMDTMAENDGARFVSLSSSAHSLTGILWDDIHFQNNSYDK 183 Query: 189 WKSYAQSKSANVLFAVALDEKAKVYGVRAFAVHPGLIPSSGIGRFLKTEEVGVKPVKVQP 248 W +Y QSK+A+ L A+ + GV F+VHPG I + + R L+ EE+ V Sbjct: 184 WMAYGQSKTASSLIAIEFHRRMVDKGVSGFSVHPGGILTP-LQRHLQKEEM------VAL 236 Query: 249 AQEDEKGKPASNTNSDFMKNTEQGAATSVWCAVSEQLAGLGGVYCEDADIAEAVPA--DS 306 DE G P+ S F K T QGA+T++WCA S L G+GGV+CED DIA+ +S Sbjct: 237 GWMDEDGSPSEMAKS-FFKTTSQGASTTLWCATSPSLNGIGGVFCEDCDIAKRKNEVDES 295 Query: 307 VQGS-GVWPWAIDREAAERLWVLSEQL 332 +Q GV WA+D E A +LW ++E++ Sbjct: 296 IQRYFGVADWAVDTEEASKLWDVTEKM 322 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.316 0.131 0.380 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 465,454,905 Number of extensions: 18698953 Number of successful extensions: 68546 Number of sequences better than 1.0e-05: 1175 Number of HSP's gapped: 69086 Number of HSP's successfully gapped: 1177 Length of query: 339 Length of database: 757,237,578 Length adjustment: 126 Effective length of query: 213 Effective length of database: 415,135,104 Effective search space: 88423777152 Effective search space used: 88423777152 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 126 (53.1 bits)