BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0566.0026.p (244 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_781299.1| lantibiotic transport ATP-binding protein spaF/... 308 9e-83 pir||I40516 spaF protein - Bacillus subtilis 305 6e-82 gb|AAB91597.1| SpaF [Bacillus subtilis] 302 4e-81 gb|AAF99691.1| MutF [Streptococcus mutans] 302 5e-81 gb|AAL15571.1| putative SpaF [Bacillus subtilis] 301 9e-81 >ref|NP_781299.1| lantibiotic transport ATP-binding protein spaF/mutF [Clostridium tetani E88] gb|AAO35236.1| lantibiotic transport ATP-binding protein spaF/mutF [Clostridium tetani E88] Length = 234 Score = 308 bits (788), Expect = 9e-83, Method: Compositional matrix adjust. Identities = 155/237 (65%), Positives = 186/237 (78%), Gaps = 6/237 (2%) Query: 2 QYILETKHLNKRFKRQQAVEDVSLKIPAGTVYGLLGPNGAGKTTTLKMITGLLRPSGGEI 61 + ILETK L K FK+QQAV++VS+ + ++YGLLGPNGAGK+T LKMI G+LRP+ GE+ Sbjct: 3 EIILETKRLCKTFKKQQAVKEVSISVQRNSIYGLLGPNGAGKSTLLKMIIGMLRPTSGEV 62 Query: 62 LLGGKPWERKQLERIGALIESPALYGNLTARENLTVHAKLLGQTAQRIDEVLHTVDLQET 121 L G W RK L +IG+LIES LY NLTA+ENL V +LG RIDEVL V+L++T Sbjct: 63 LFDGHKWSRKDLIKIGSLIESAPLYENLTAKENLKVRTTVLGLPDSRIDEVLSIVELEDT 122 Query: 122 GSKKASQFSLGMKQRLGIAIALLNRPELLILDEPTNGLDPLGIQELRELIRSFPAQGITV 181 G K+A QFS+GMKQRLGIAIALLN P+LLILDEPTNGLDP GIQELRELIRSFP++GITV Sbjct: 123 GKKRAGQFSMGMKQRLGIAIALLNNPKLLILDEPTNGLDPFGIQELRELIRSFPSKGITV 182 Query: 182 ILSSHILSEVEQIADYIXXXXXXXIISGGRLGHESKIHKGEDLETLFMQVVARNREQ 238 ILSSHILSEVEQIAD+I IISGG LG+E ++ KGEDLE LFM + + R + Sbjct: 183 ILSSHILSEVEQIADHI------GIISGGVLGYEGELKKGEDLEKLFMSIAGKYRRE 233 >pir||I40516 spaF protein - Bacillus subtilis Length = 456 Score = 305 bits (781), Expect = 6e-82, Method: Compositional matrix adjust. Identities = 152/238 (63%), Positives = 186/238 (78%), Gaps = 6/238 (2%) Query: 4 ILETKHLNKRFKRQQAVEDVSLKIPAGTVYGLLGPNGAGKTTTLKMITGLLRPSGGEILL 63 +LETK L K+F ++ AV DVSL + ++YGLLGPNGAGK+TTLK++TG+LR + GEIL Sbjct: 14 LLETKSLTKKFGKETAVADVSLTVQKNSIYGLLGPNGAGKSTTLKILTGILRKTSGEILF 73 Query: 64 GGKPWERKQLERIGALIESPALYGNLTARENLTVHAKLLGQTAQRIDEVLHTVDLQETGS 123 G W+R L+ IG+LIE+P LY NLTA EN+ VHA LLG + RI VL TVD+Q G Sbjct: 74 DGHKWKRSDLQTIGSLIEAPPLYDNLTAFENVKVHATLLGLSEDRIHSVLKTVDMQHAGK 133 Query: 124 KKASQFSLGMKQRLGIAIALLNRPELLILDEPTNGLDPLGIQELRELIRSFPAQGITVIL 183 K+A QFS+GM+QRLGIAIALLN P+LLILDEPTNGLDP+GIQELR+LIRSFP +GITVIL Sbjct: 134 KRAGQFSMGMRQRLGIAIALLNHPKLLILDEPTNGLDPIGIQELRDLIRSFPQEGITVIL 193 Query: 184 SSHILSEVEQIADYIXXXXXXXIISGGRLGHESKIHKGEDLETLFMQVVARNREQAGV 241 SSHILSEVEQIAD+I II G LG++ +I+K +DLE LFM+VV RE+ GV Sbjct: 194 SSHILSEVEQIADHI------GIIIDGHLGYQGEINKADDLEHLFMEVVKSTREKEGV 245 >gb|AAB91597.1| SpaF [Bacillus subtilis] Length = 247 Score = 302 bits (774), Expect = 4e-81, Method: Compositional matrix adjust. Identities = 152/238 (63%), Positives = 186/238 (78%), Gaps = 6/238 (2%) Query: 4 ILETKHLNKRFKRQQAVEDVSLKIPAGTVYGLLGPNGAGKTTTLKMITGLLRPSGGEILL 63 +LETK L K+F ++ AV DVSL + ++YGLLGPNGAGK+TTLK++TG+LR + GEIL Sbjct: 14 LLETKSLTKKFGKETAVADVSLTVQKNSIYGLLGPNGAGKSTTLKILTGILRKTSGEILF 73 Query: 64 GGKPWERKQLERIGALIESPALYGNLTARENLTVHAKLLGQTAQRIDEVLHTVDLQETGS 123 G W+R L+ IG+LIE+P LY NLTA EN+ VHA LLG + RI VL TVD+Q G Sbjct: 74 DGHKWKRSDLQTIGSLIEAPPLYDNLTAFENVKVHATLLGLSEDRIHSVLKTVDMQHAGK 133 Query: 124 KKASQFSLGMKQRLGIAIALLNRPELLILDEPTNGLDPLGIQELRELIRSFPAQGITVIL 183 K+A QFS+GM+QRLGIAIALLN P+LLILDEPTNGLDP+GIQELR+LIRSFP +GITVIL Sbjct: 134 KRAGQFSMGMRQRLGIAIALLNHPKLLILDEPTNGLDPIGIQELRDLIRSFPQEGITVIL 193 Query: 184 SSHILSEVEQIADYIXXXXXXXIISGGRLGHESKIHKGEDLETLFMQVVARNREQAGV 241 SSHILSEVEQIAD+I II G LG++ +I+K +DLE LFM+VV RE+ GV Sbjct: 194 SSHILSEVEQIADHI------GIIIDGHLGYQGEINKADDLEHLFMEVVKSTREKEGV 245 >gb|AAF99691.1| MutF [Streptococcus mutans] Length = 233 Score = 302 bits (773), Expect = 5e-81, Method: Compositional matrix adjust. Identities = 153/236 (64%), Positives = 181/236 (76%), Gaps = 6/236 (2%) Query: 1 MQYILETKHLNKRFKRQQAVEDVSLKIPAGTVYGLLGPNGAGKTTTLKMITGLLRPSGGE 60 M Y+LETK+L K+F +Q AV ++LK+ ++YGLLGPNG+GK+TTLKMITG+LR + G Sbjct: 1 MDYMLETKNLTKQFGKQTAVNQLNLKVERHSIYGLLGPNGSGKSTTLKMITGMLRKTSGH 60 Query: 61 ILLGGKPWERKQLERIGALIESPALYGNLTARENLTVHAKLLGQTAQRIDEVLHTVDLQE 120 IL+ G W RK LE IGALIESP LY NLTARENL V +LG RIDEVL VDL Sbjct: 61 ILIDGHDWSRKDLENIGALIESPPLYENLTARENLKVRTLMLGLPDSRIDEVLKIVDLTN 120 Query: 121 TGSKKASQFSLGMKQRLGIAIALLNRPELLILDEPTNGLDPLGIQELRELIRSFPAQGIT 180 TG K+A QFS+GMKQRLGIAIALLN P+LLILDEPTNGLDP+GIQELR LIRSFP QGIT Sbjct: 121 TGKKRAGQFSMGMKQRLGIAIALLNSPQLLILDEPTNGLDPIGIQELRNLIRSFPTQGIT 180 Query: 181 VILSSHILSEVEQIADYIXXXXXXXIISGGRLGHESKIHKGEDLETLFMQVVARNR 236 VI+SSHILSE++ AD+I II+ G LG++ +IH+ EDLE LF VV R R Sbjct: 181 VIISSHILSEIQMTADHI------GIIANGVLGYQDRIHQDEDLEKLFTDVVMRYR 230 >gb|AAL15571.1| putative SpaF [Bacillus subtilis] Length = 247 Score = 301 bits (771), Expect = 9e-81, Method: Compositional matrix adjust. Identities = 150/237 (63%), Positives = 187/237 (78%), Gaps = 6/237 (2%) Query: 4 ILETKHLNKRFKRQQAVEDVSLKIPAGTVYGLLGPNGAGKTTTLKMITGLLRPSGGEILL 63 +LETK L K+F ++ AV DVSL + ++YGLLGPNGAGK+TTLK++TG+LR + GEI Sbjct: 14 LLETKGLTKKFGKETAVADVSLTVQKNSIYGLLGPNGAGKSTTLKILTGILRKTSGEIFF 73 Query: 64 GGKPWERKQLERIGALIESPALYGNLTARENLTVHAKLLGQTAQRIDEVLHTVDLQETGS 123 G W+R L+ IG+LIESP LY NLTA EN+ VHA LLG + +RI+ VL TVD+ G Sbjct: 74 DGHKWKRSDLKNIGSLIESPPLYDNLTAFENVKVHATLLGLSKERINSVLETVDMLHAGK 133 Query: 124 KKASQFSLGMKQRLGIAIALLNRPELLILDEPTNGLDPLGIQELRELIRSFPAQGITVIL 183 K+A QFS+GM+QRLGIAIALLN P+LLILDEPTNGLDP+GIQELR+LIRSFP +GITVIL Sbjct: 134 KRAGQFSMGMRQRLGIAIALLNHPKLLILDEPTNGLDPIGIQELRDLIRSFPLEGITVIL 193 Query: 184 SSHILSEVEQIADYIXXXXXXXIISGGRLGHESKIHKGEDLETLFMQVVARNREQAG 240 SSHILSEVEQIAD+I IIS G LG++ +I+K ++LE LFM+VV +RE+ G Sbjct: 194 SSHILSEVEQIADHI------GIISDGHLGYQGEINKADNLEQLFMEVVKSSREKGG 244 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.317 0.137 0.381 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 336,523,059 Number of extensions: 13841319 Number of successful extensions: 107675 Number of sequences better than 1.0e-05: 19772 Number of HSP's gapped: 88562 Number of HSP's successfully gapped: 21035 Length of query: 244 Length of database: 757,237,578 Length adjustment: 122 Effective length of query: 122 Effective length of database: 425,995,500 Effective search space: 51971451000 Effective search space used: 51971451000 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 124 (52.4 bits)