BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0564.0053.p (287 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_00183277.1| hypothetical protein Exigu133501 [Exiguobacte... 200 2e-50 gb|AAR88433.1| 17b-hydroxysteroid dehydrogenase type I [Anguilla... 124 2e-27 pir||JC7561 3(or 17)beta-hydroxysteroid dehydrogenase (EC 1.1.1.... 124 3e-27 ref|NP_991147.1| 17-beta hydroxysteroid dehydrogenase [Danio rer... 122 9e-27 gb|EAD03765.1| unknown [environmental sequence] 122 1e-26 >ref|ZP_00183277.1| hypothetical protein Exigu133501 [Exiguobacterium sp. 255-15] Length = 171 Score = 200 bits (509), Expect = 2e-50, Method: Compositional matrix adjust. Identities = 90/170 (52%), Positives = 129/170 (75%), Gaps = 1/170 (0%) Query: 119 MHTIRAALPLLRKTGYG-HIVATSSVGGLVGQPMNEIYCASKFALEGLLESMATYYKPQF 177 M TI LPLL+ G H++ATSS+ GLVGQPMNEIYCASKFALEGL ES+ATYYKP F Sbjct: 1 MRTIECCLPLLKAAPNGSHLLATSSISGLVGQPMNEIYCASKFALEGLFESLATYYKPFF 60 Query: 178 NIDISLLEPGAIATEFNNTVLGHVSETGGILDDEYKPVIEAYTQAFLQRNVEPQTADSVA 237 +ID++L+EP A+ T F +VL + +TGG+ +D++KP+IE+Y + + R+ E Q+ +++A Sbjct: 61 SIDVTLIEPAAVETNFTASVLDRLEQTGGLHEDDFKPIIESYLRTYRVRHAERQSPETIA 120 Query: 238 EVMWNLVHMEPKPLRIRTSEQAENFVSRKVSTDPTGLEGVLSTRKIQLNM 287 E+++ LVH + KPLR+RT+ + E FV+ K + DP+GL G L TR++ LN+ Sbjct: 121 ELIFKLVHADEKPLRVRTAPEDEAFVAHKTANDPSGLVGTLKTRQLTLNL 170 >gb|AAR88433.1| 17b-hydroxysteroid dehydrogenase type I [Anguilla japonica] Length = 293 Score = 124 bits (311), Expect = 2e-27, Method: Compositional matrix adjust. Identities = 78/245 (31%), Positives = 127/245 (51%), Gaps = 17/245 (6%) Query: 35 WTVYAGTRNLERDQERYREV------EHLTFVELEVTKPESIQRVIGQIEQEQDKLDVLF 88 + VYA RNL + +ER E + L ++++VT +SIQ +I + +++D+L Sbjct: 31 YKVYASMRNLAK-KERLLEYVKGFHKDTLDIIQMDVTDQQSIQDAKEKITE--NRVDILV 87 Query: 89 CNAGFGYLRALGQAPVEDIHRVFDTNVYGVMHTIRAALPLLRKTGYGHIVATSSVGGLVG 148 CNAG G + L + + ++ D N+ G + TI+ LP ++ GH++ T S+GGL G Sbjct: 88 CNAGVGLMGPLETLSQDTMRQILDVNLLGTIRTIQTFLPGMKTRRRGHVLVTGSIGGLQG 147 Query: 149 QPMNEIYCASKFALEGLLESMATYYKPQFNIDISLLEPGAIATEFNNTVLGHVSETGGIL 208 P NE+YCASKFA+EG+ ES+A + FNI +SL+E G + T F + + SE+G Sbjct: 148 LPFNELYCASKFAVEGVCESLAILLQ-HFNIHVSLIESGPVNTHFLSNL--RRSESGDAA 204 Query: 209 DDEYKPVIEAYTQAFLQR-----NVEPQTADSVAEVMWNLVHMEPKPLRIRTSEQAENFV 263 + P+ +LQ + Q A + +V + LR T+ Sbjct: 205 LLQVDPLTRDLYDTYLQHCSSVFHSAAQDAQDIVQVFLEAIQSPSPSLRYYTNSALLPLT 264 Query: 264 SRKVS 268 SRK++ Sbjct: 265 SRKLA 269 >pir||JC7561 3(or 17)beta-hydroxysteroid dehydrogenase (EC 1.1.1.51) I - Japanese eel Length = 293 Score = 124 bits (310), Expect = 3e-27, Method: Compositional matrix adjust. Identities = 76/244 (31%), Positives = 125/244 (51%), Gaps = 15/244 (6%) Query: 35 WTVYAGTRNLERDQERYREV-----EHLTFVELEVTKPESIQRVIGQIEQEQDKLDVLFC 89 + VYA RNL + + V + L ++++VT +SIQ +I + +++D+L C Sbjct: 31 YKVYASMRNLAKKERLLTYVKGFHKDTLDIIQMDVTDQQSIQDAKEKITE--NRVDILVC 88 Query: 90 NAGFGYLRALGQAPVEDIHRVFDTNVYGVMHTIRAALPLLRKTGYGHIVATSSVGGLVGQ 149 NAG G + L + + ++ D N+ G + TI+ LP ++ GH++ T S+GGL G Sbjct: 89 NAGVGLMGPLETLSQDTMRQILDVNLLGTIRTIQTFLPGMKTRPSGHVLVTGSIGGLQGL 148 Query: 150 PMNEIYCASKFALEGLLESMATYYKPQFNIDISLLEPGAIATEFNNTVLGHVSETGGILD 209 P NE+YCASKFA+EG+ ES+A + FNI +SL+E G + T F + + SE+G Sbjct: 149 PFNELYCASKFAVEGVCESLAILLQ-HFNIHVSLIESGPVNTHFLSNL--RRSESGDAAL 205 Query: 210 DEYKPVIEAYTQAFLQR-----NVEPQTADSVAEVMWNLVHMEPKPLRIRTSEQAENFVS 264 + P+ +LQ + Q A + +V + LR T+ S Sbjct: 206 LQVDPLTRDLYDTYLQHCSSVFHSAAQDAQDIVQVFLEAIQSPSPSLRYYTNSALLPLTS 265 Query: 265 RKVS 268 RK++ Sbjct: 266 RKLA 269 >ref|NP_991147.1| 17-beta hydroxysteroid dehydrogenase [Danio rerio] gb|AAP74564.1| 17-beta hydroxysteroid dehydrogenase [Danio rerio] Length = 295 Score = 122 bits (306), Expect = 9e-27, Method: Compositional matrix adjust. Identities = 71/196 (36%), Positives = 113/196 (57%), Gaps = 13/196 (6%) Query: 33 EGWTVYAGTRNLERDQERYREV-----EHLTFVELEVTKPESIQRVIGQIEQEQDKLDVL 87 + + VYA RNL++ Q V + L ++++VT +SI + Q + ++D+L Sbjct: 29 KAYKVYATMRNLDKKQRLLESVRGLHKDTLDILQMDVTDQQSI--LDAQRNVSEGRIDIL 86 Query: 88 FCNAGFGYLRALGQAPVEDIHRVFDTNVYGVMHTIRAALPLLRKTGYGHIVATSSVGGLV 147 CNAG G + L ++ I + D N+ G + TI+ LP ++K +G I+ T S+GGL Sbjct: 87 VCNAGVGLMGPLETHSLDTIRAIMDVNLLGTIRTIQTFLPDMKKKRHGRILVTGSMGGLQ 146 Query: 148 GQPMNEIYCASKFALEGLLESMATYYKPQFNIDISLLEPGAIATEFNNTVLGHVSETGGI 207 G P NE+YCASKFA+EG ES+A + FNI ISL+E G + T+F + +ETG Sbjct: 147 GLPFNEVYCASKFAIEGACESLAILLQ-HFNIHISLIECGPVNTDFLMNL--KRTETG-- 201 Query: 208 LDDEYKPVIEAYTQAF 223 D E + ++A+T++ Sbjct: 202 -DKELEVEVDAHTRSL 216 >gb|EAD03765.1| unknown [environmental sequence] Length = 271 Score = 122 bits (305), Expect = 1e-26, Method: Compositional matrix adjust. Identities = 86/268 (32%), Positives = 135/268 (50%), Gaps = 11/268 (4%) Query: 9 MGKIICITXXXXXXXXXXXXKFAKEGWTVYAGTRNLERDQERYREVEH----LTFVELEV 64 M K+ +T A+EG+ YA R++++ E + + L +EL+V Sbjct: 1 MEKVAVVTGSSSGIGFETSLALAREGYFTYATMRDVKKAAEIQKITDEESLPLKIIELDV 60 Query: 65 TKPESIQRVIGQIEQEQDKLDVLFCNAGFGYLRALGQAPVEDIHRVFDTNVYGVMHTIRA 124 ES + I I QE+D++DVL NAG+G +E+ F+TN + V+ I+ Sbjct: 61 DNEESAENAINTIIQEKDRIDVLVNNAGWGIWGTGEDVSIEEFKAQFETNFFSVVRMIQK 120 Query: 125 ALPLLRKTGYGHIVATSSVGGLVGQPMNEIYCASKFALEGLLESMATYYKPQFNIDISLL 184 P +RK G G+IV SSV G +G P + Y +SKFA+EGL ES+ Y QF I++ ++ Sbjct: 121 VAPTMRKQGSGNIVNISSVAGRIGLPASTAYVSSKFAVEGLSESL-RYELGQFGINVIII 179 Query: 185 EPGAIATEFNNTV-LGHVSETGGILDDEYKPVIEAYTQAFLQRNVEPQTADSVAEVMWNL 243 EPG I T F +++ ++TG D VI + + P+ VA+V+ N Sbjct: 180 EPGVIKTNFFDSMKTAKKADTGETYRDITLKVITG-VKMMAEMGTSPK---EVAQVIINS 235 Query: 244 VHME-PKPLRIRTSEQAENFVSRKVSTD 270 + E P P I ++ A S+K+ TD Sbjct: 236 LKEEKPLPRYIVGNDAAMFMESKKMKTD 263 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.318 0.134 0.385 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 395,124,682 Number of extensions: 16104997 Number of successful extensions: 48112 Number of sequences better than 1.0e-05: 2821 Number of HSP's gapped: 48954 Number of HSP's successfully gapped: 2824 Length of query: 287 Length of database: 757,237,578 Length adjustment: 124 Effective length of query: 163 Effective length of database: 420,565,302 Effective search space: 68552144226 Effective search space used: 68552144226 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 125 (52.8 bits)