BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0564.0031.p (404 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gb|EAK11751.1| unknown [environmental sequence] 232 1e-59 gb|EAK40791.1| unknown [environmental sequence] 219 6e-56 gb|EAK41547.1| unknown [environmental sequence] 206 5e-52 ref|NP_718681.1| oxidoreductase, Gfo/Idh/MocA family [Shewanella... 184 4e-45 gb|EAJ56971.1| unknown [environmental sequence] 181 2e-44 >gb|EAK11751.1| unknown [environmental sequence] Length = 417 Score = 232 bits (591), Expect = 1e-59, Method: Compositional matrix adjust. Identities = 144/423 (34%), Positives = 216/423 (51%), Gaps = 47/423 (11%) Query: 6 IRLAIVG-GNRGSRFYDLLAWIADRIRLAAICDVDDRILS---NWKEQ--YPSIQTYQN- 58 ++L +G G RG L + D + + ICD+DD +++ N +Q P + Y N Sbjct: 1 MKLGFIGTGLRGQGMMRLATYRND-VDIPVICDIDDGMINKALNVLKQAGRPVPKIYNNG 59 Query: 59 ---YEQLLQNPDIDAVFLASPMLLHARQSIDALKAGKHVLSEVIAAHTVEEAWELVETVE 115 ++ +L N D+D VF+A+P H S+ A+KAGKHV +EV AA T+ E W+LV T E Sbjct: 60 DEDFQNMLDNEDMDGVFIATPWEWHHPMSVAAMKAGKHVGTEVPAALTLAECWDLVNTSE 119 Query: 116 QTGLVYMMAENYCFSRTSLMIRHMAEQGLFGEITYMEGGYIHDLRALVH----QPDGS-- 169 ++G M+ EN C+ R L + +M GLFGE+ + +GGY HDLR + QP G Sbjct: 120 KSGKFCMIMENVCYRRDVLAVLNMVRSGLFGELIHCQGGYQHDLRNVKFNDGLQPYGGGV 179 Query: 170 ---------LNWRGQLHHTYNGMNYPTHSLGPLAGWIAVNKQGGDQLESISTYVSNSRAL 220 WR NG YPTH LGP++ + +N G+++ +++ + SR L Sbjct: 180 EFGDKGYSESKWRTNHSVNRNGDLYPTHGLGPVSTMLDINH--GNRMVYLTSTATQSRGL 237 Query: 221 RKYFHEQFGGNHPAAQDGYWKQGDCAVTAVRTRGGVLITLRVDWTSVRPHNMRHYALQGT 280 Y E G NHP A + K GD T ++ G I L D S RP+++ ++ +QGT Sbjct: 238 HNYIIEHGGPNHPNASINF-KLGDVVTTVIKCANGQTIMLSHDTNSPRPYSL-NFRVQGT 295 Query: 281 AGAYLSARHDQEGDLIWLRDRSPIADDGAD----KWESLERYRPEYDHPLWKQWXXXXXX 336 G +W++D + I +G +WES ++Y +YDHPLWK++ Sbjct: 296 KG-------------LWMKDNNSIYIEGVSPKNHRWESDKKYLQQYDHPLWKRFENDAKG 342 Query: 337 XXXXXXDFLVMEEFASAITEQRSPAIDVYDAVTWSSIFPLSMESVAAGGKPLAIPDFARN 396 D+ V F +I P +DVYDA +WS I PLS +S+ + IPDF R Sbjct: 343 SGHGGMDYFVFRAFIESIKRDVDPPLDVYDAASWSVISPLSEKSIRKNSASIKIPDFTRG 402 Query: 397 RGK 399 + K Sbjct: 403 KWK 405 >gb|EAK40791.1| unknown [environmental sequence] Length = 450 Score = 219 bits (559), Expect = 6e-56, Method: Compositional matrix adjust. Identities = 147/428 (34%), Positives = 215/428 (50%), Gaps = 52/428 (12%) Query: 3 QQPIRLAIVG-GNRGSRFYDLLAWIADRIRLAAICDVDDRILSNWKEQY-------PSI- 53 Q +++ I+G G RG LL D + + AICD+D R++ E + P I Sbjct: 28 NQRLQIGIIGTGLRGQWNAKLLLDRPD-VDIPAICDIDQRMIDMVLELFSKAGRPKPKIY 86 Query: 54 -QTYQNYEQLLQNPDIDAVFLASPMLLHARQSIDALKAGKHVLSEVIAAHTVEEAWELVE 112 Q +Y +L N D+DAV +A+P H SI+A+K HV EV AA T+ + W+LV Sbjct: 87 DQGPDDYLNMLSNEDLDAVNIATPWEWHHPMSIEAMKNKMHVGVEVPAALTINDCWDLVN 146 Query: 113 TVEQTGLVYMMAENYCFSRTSLMIRHMAEQGLFGEITYMEGGYIHDLRALVHQPDGS--- 169 T E+T + M+ EN + R + I +M +GLFGE+ + +GGY HDLR V DG+ Sbjct: 147 TSEKTKKLCMIMENVNYRRDIMAILNMVREGLFGELIHCQGGYQHDLRH-VKFNDGTGPY 205 Query: 170 -------------LNWRGQLHHTYNGMNYPTHSLGPLAGWIAVNKQGGDQLESISTYVSN 216 WR Q N YPTH LGP++ + +N+ G+++ +++ S Sbjct: 206 GGGVEFGAKAFSEARWRTQHSVEKNADLYPTHGLGPISPMLNINR--GNRMLRLTSTASQ 263 Query: 217 SRALRKYFHEQFGGNHPAAQDGYWKQGDCAVTAVRTRGGVLITLRVDWTSVRPHNMRHYA 276 SR L KY E G +HP A+ + K GD T ++ G I L D S RP+++ ++ Sbjct: 264 SRGLNKYIIENGGSDHPNAKIQF-KLGDVVTTVIKCAKGQTIVLSHDTNSPRPYSL-NFR 321 Query: 277 LQGTAGAYLSARHDQEGDLIWLRDRSPIADDG----ADKWESLERYRPEYDHPLWKQWXX 332 +QGT G IW++D I +G + +WE + Y EYDHPLWK++ Sbjct: 322 VQGTKG-------------IWMKDSKSIYIEGKSPESHRWEKDQPYLAEYDHPLWKRFSK 368 Query: 333 XXXXXXXX---XXDFLVMEEFASAITEQRSPAIDVYDAVTWSSIFPLSMESVAAGGKPLA 389 DF ++ F AI + P IDVYDAV+ S I PLS +S+ + Sbjct: 369 AKSTLSHTGHGGMDFFIIRAFVEAIKGDQRPVIDVYDAVSMSCIVPLSEQSIKNNSASVK 428 Query: 390 IPDFARNR 397 IPDF R R Sbjct: 429 IPDFTRGR 436 >gb|EAK41547.1| unknown [environmental sequence] Length = 420 Score = 206 bits (525), Expect = 5e-52, Method: Compositional matrix adjust. Identities = 135/427 (31%), Positives = 214/427 (50%), Gaps = 56/427 (13%) Query: 6 IRLAIVG-GNRGSRFYDLLAWIADR---IRLAAICDVDD-------RILSNWKEQYPSI- 53 ++L +G G RG + W+A + + + AICD+D+ +IL + P I Sbjct: 5 VKLGFIGTGLRGQ----WMLWLAAKYPEVEIPAICDIDEQMIASALKILKDAGRPEPRIY 60 Query: 54 -QTYQNYEQLLQNPDIDAVFLASPMLLHARQSIDALKAGKHVLSEVIAAHTVEEAWELVE 112 + +++ +L N +D V++A+P H +I A+ G HV +EV AA TV+E W+LV Sbjct: 61 KKNEEDFRTMLDNETLDGVYIATPWEWHHPMAIAAMNNGIHVGTEVPAALTVKECWDLVN 120 Query: 113 TVEQTGLVYMMAENYCFSRTSLMIRHMAEQGLFGEITYMEGGYIHDLRALVHQPDG---- 168 E+T M+ EN C+ R + + +M + +FGE+ + +GGY HDLR V DG Sbjct: 121 VSEKTNKFCMIMENVCYRRDIMAVLNMVRKNMFGELLHCQGGYQHDLRH-VKFNDGINPY 179 Query: 169 ------------SLNWRGQLHHTYNGMNYPTHSLGPLAGWIAVNKQGGDQLESISTYVSN 216 WR Q NG YPTH LGP++ + +N+ G+++ +++ + Sbjct: 180 GGGVEFGEKGFSEAKWRTQHSIDRNGDLYPTHGLGPVSPMLDINR--GNRMLHLTSTATQ 237 Query: 217 SRALRKYFHEQFGGNHPAAQDGYWKQGDCAVTAVRTRGGVLITLRVDWTSVRPHNMRHYA 276 SR L KY ++ G +HP A + K GD T ++ G I L D S RP+++ ++ Sbjct: 238 SRGLHKYIVDKGGKDHPNAAIDF-KCGDIVTTVIKCAQGQTIMLSHDTNSPRPYSL-NFR 295 Query: 277 LQGTAGAYLSARHDQEGDLIWLRDRSPIADDGADK----WESLERYRPEYDHPLWKQWXX 332 +QGT G IW +D I +G K WE ++Y EYDHPLWK++ Sbjct: 296 VQGTQG-------------IWQKDARSIYIEGVSKEEHRWEEEDQYLAEYDHPLWKRFED 342 Query: 333 XXXXXXXXXXDFLVMEEFASAITEQRSPAIDVYDAVTWSSIFPLSMESVAAGGKPLAIPD 392 DF ++ F A+ + P +DVYDA + S + PLS +S+ G + +PD Sbjct: 343 QAEGSGHGGMDFFILRAFIEAL-KGAEPILDVYDAASMSVVSPLSEKSIRLGSAAVKVPD 401 Query: 393 FARNRGK 399 F R + K Sbjct: 402 FTRGKWK 408 >ref|NP_718681.1| oxidoreductase, Gfo/Idh/MocA family [Shewanella oneidensis MR-1] gb|AAN56125.1| oxidoreductase, Gfo/Idh/MocA family [Shewanella oneidensis MR-1] Length = 459 Score = 184 bits (466), Expect = 4e-45, Method: Compositional matrix adjust. Identities = 125/409 (30%), Positives = 204/409 (49%), Gaps = 37/409 (9%) Query: 6 IRLAIVG-GNRGSRFYDLLAWIADRIRLAAICD-----VDDRILSNWKEQYPSIQTYQ-- 57 +R+ +G G RG + + + + L AICD VD + K++ P Y Sbjct: 55 VRVGFIGVGERGFSHVEQFCHL-EGVELKAICDTHQAVVDRAVEHIVKQKRPKPAVYTGN 113 Query: 58 --NYEQLLQNPDIDAVFLASPMLLHARQSIDALKAGKHVLSEVIAAHTVEEAWELVETVE 115 +Y +LL DID V +++P HA +ID +++GKH EV A TVEE W++++T E Sbjct: 114 DLSYRELLNRDDIDIVIISTPWEWHAPMAIDTMESGKHAFVEVPLALTVEECWQIIDTAE 173 Query: 116 QTGLVYMMAENYCFSRTSLMIRHMAEQGLFGEITYMEGGYIHDLR---ALVHQPDGSLNW 172 +T MM EN + R LM+ +M QGLFGE+ + E YIH+LR ++ GS W Sbjct: 174 RTQKNCMMMENVNYGREELMVLNMVRQGLFGELLHGEAAYIHELRWQMKEINHKTGS--W 231 Query: 173 RGQLHHTYNGMNYPTHSLGPLAGWIAVNKQGGDQLESISTYVSNSRALRKYFHEQFGGNH 232 R H NG YPTH LGP++ ++ +N+ GD+ + +++ S + Y +F +H Sbjct: 232 RTYWHTKRNGNLYPTHGLGPVSQYMNINR--GDRFDYLTSMSSPALGRALYAKREFPADH 289 Query: 233 PAAQDGYWKQGDCAVTAVRTRGGVLITLRVDWTSVRPHNMRHYALQGTAGAYLSARHDQE 292 Q Y GD + + ++T G I ++ D T+ RP++ RH +QGT G + Sbjct: 290 ERNQLKY-INGDMSTSLIKTVKGRTIMVQHDTTTPRPYS-RHNLIQGTNGVFAG------ 341 Query: 293 GDLIWLRDRSPIADDGA----DKWES-LERYRPEYDHPLWKQW-XXXXXXXXXXXXDFLV 346 +R + +DG KW++ ++++ +YDHPLW++ DF++ Sbjct: 342 -----FPNRIAVENDGFGTSYHKWDTDMQKWYDKYDHPLWQRIGKEAEINGGHGGMDFVM 396 Query: 347 MEEFASAITEQRSPAIDVYDAVTWSSIFPLSMESVAAGGKPLAIPDFAR 395 + + + DVYD +WS + LS +S+ + PDF R Sbjct: 397 LWRMVYCLRNGEALDQDVYDGASWSVVNILSEQSLNNRSNSVNFPDFTR 445 >gb|EAJ56971.1| unknown [environmental sequence] Length = 459 Score = 181 bits (460), Expect = 2e-44, Method: Compositional matrix adjust. Identities = 126/407 (30%), Positives = 204/407 (50%), Gaps = 33/407 (8%) Query: 6 IRLAIVG-GNRGSRFYDLLAWIADRIRLAAICD----VDDRILSNWKEQY---PSIQTYQ 57 +R+ +G G RG + + D + L AICD V DR + + +Q P+I T Sbjct: 55 VRVGFIGVGERGFSHVEQFCHL-DGVELKAICDTHQAVIDRAVEHIVKQKRPKPAIYTGN 113 Query: 58 N--YEQLLQNPDIDAVFLASPMLLHARQSIDALKAGKHVLSEVIAAHTVEEAWELVETVE 115 + Y +LL DID V +++P HA +ID +++GKH EV A TVEE W++++T E Sbjct: 114 DLSYRELLNRDDIDIVIISTPWEWHAPMAIDTMESGKHAFVEVPLALTVEECWQIIDTAE 173 Query: 116 QTGLVYMMAENYCFSRTSLMIRHMAEQGLFGEITYMEGGYIHDLRALVHQPDGSL-NWRG 174 +T MM EN + R LM+ +M QGLFGE+ + E YIH+LR + + D +WR Sbjct: 174 RTQKNCMMMENVNYGREELMVLNMVRQGLFGELLHGEAAYIHELRWQMKEIDHKTGSWRT 233 Query: 175 QLHHTYNGMNYPTHSLGPLAGWIAVNKQGGDQLESISTYVSNSRALRKYFHEQFGGNHPA 234 H NG YPTH LGP++ ++ +N+ GD+ + +++ S + Y +F +H Sbjct: 234 YWHTKRNGNLYPTHGLGPISQYMNINR--GDRFDYLTSMSSPALGRALYAKREFPADHER 291 Query: 235 AQDGYWKQGDCAVTAVRTRGGVLITLRVDWTSVRPHNMRHYALQGTAGAYLSARHDQEGD 294 Q Y GD + + ++T G I ++ D T+ RP++ RH +QGT G + Sbjct: 292 NQLKY-INGDMSTSLIKTVKGRTIMVQHDTTTPRPYS-RHNLIQGTNGVFAG-------- 341 Query: 295 LIWLRDRSPIADDGADK----WE-SLERYRPEYDHPLWKQW-XXXXXXXXXXXXDFLVME 348 +R I + G + W+ ++++ +YDHPLW++ DF+++ Sbjct: 342 ---FPNRIAIENGGFGQSYHEWDMDMQKWYDKYDHPLWQRIGKEAEINGGHGGMDFVMLW 398 Query: 349 EFASAITEQRSPAIDVYDAVTWSSIFPLSMESVAAGGKPLAIPDFAR 395 + + DVYD WS + LS +S+ + PDF R Sbjct: 399 RMVYCLRNGEALDQDVYDGAAWSVVNILSEQSLNNRSNSVNFPDFTR 445 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.320 0.135 0.423 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 618,474,675 Number of extensions: 24881322 Number of successful extensions: 68460 Number of sequences better than 1.0e-05: 405 Number of HSP's gapped: 68441 Number of HSP's successfully gapped: 405 Length of query: 404 Length of database: 757,237,578 Length adjustment: 128 Effective length of query: 276 Effective length of database: 409,704,906 Effective search space: 113078554056 Effective search space used: 113078554056 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 127 (53.5 bits)