BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0563.0003.p (396 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_00048044.1| COG1785: Alkaline phosphatase [Magnetospirill... 179 1e-43 gb|EAK08759.1| unknown [environmental sequence] 173 6e-42 gb|EAJ14886.1| unknown [environmental sequence] 171 2e-41 gb|EAH99728.1| unknown [environmental sequence] 166 1e-39 gb|EAJ74642.1| unknown [environmental sequence] 164 4e-39 >ref|ZP_00048044.1| COG1785: Alkaline phosphatase [Magnetospirillum magnetotacticum] Length = 210 Score = 179 bits (453), Expect = 1e-43, Method: Compositional matrix adjust. Identities = 91/176 (51%), Positives = 118/176 (67%), Gaps = 5/176 (2%) Query: 15 KTKNVILFVGDGMGTAARNAIRLATVGEKGKLAMDDMPYAGLVHTSSTVP---VTDXXXX 71 + +NVI GDG+G + R IRLATVG+ G+LAMD + +AG T S P VTD Sbjct: 30 RARNVIFIQGDGLGLSHRELIRLATVGKDGQLAMDSLEHAGWTTTDSADPEEAVTDSAAG 89 Query: 72 XXXXXXGVKTYNGAIGMDANKKSVKTIAEYAKEAGKSTGVVTTSQITDATGAAFGAHVED 131 GV+TYNGA+G+D + V T+ E A++AGK+TG+VTT+Q+TDAT AAFGAHV D Sbjct: 90 ATAFASGVRTYNGAVGVDVDGNPVPTLLEAARDAGKATGLVTTAQVTDATPAAFGAHVPD 149 Query: 132 RSKQSDIALQLLTKSKVDVLLGGGEDFWYPAGEAGKFPDAPAEDPSEKSKGTQGNL 187 R QS+IA QLL S+ DV+LGGGED+WYP GE G + D PA + ++ QGN Sbjct: 150 RGDQSEIARQLLEGSRPDVVLGGGEDWWYPGGEPGAWEDNPAR--TRRADRAQGNW 203 >gb|EAK08759.1| unknown [environmental sequence] Length = 365 Score = 173 bits (439), Expect = 6e-42, Method: Compositional matrix adjust. Identities = 130/385 (33%), Positives = 189/385 (49%), Gaps = 40/385 (10%) Query: 15 KTKNVILFVGDGMGTAARNAIRLATVGEKGKLAMDDMPYAGLV--HTSSTVPVTDXXXXX 72 K +N+IL +GDGMG + R G KL+ D ++G V HT +++ VTD Sbjct: 7 KARNIILLIGDGMGPTHISVYRNVHGGPNHKLSFDKFDFSGYVKTHTYNSL-VTDSAASA 65 Query: 73 XXXXXGVKTYNGAIGMDANKKSVKTIAEYAKEAGKSTGVVTTSQITDATGAAFGAHVEDR 132 GVKT N +G+D NKK K I E E G +++TS+IT AT AAF +HV R Sbjct: 66 TAFSSGVKTINRYVGVDHNKKPAKNITEMLFEKGFVNTIISTSEITHATPAAFASHVSSR 125 Query: 133 SKQSDIALQLLTKSKVDVLLGGGEDFWYPAGEAGKFPDAPAEDPSEKSKGTQGNLVTKAK 192 ++ +IA + + SK ++LGGG D++ P E G D D +K K L TK Sbjct: 126 YEKEEIA-EHIYNSKNHIVLGGGTDYFLPIEEGGIREDDV--DYVKKIKKNFHYLETKKD 182 Query: 193 QLGYNYVSTXXXXXXXXXXXXXXXFANEEMFQQNEEGKGDIYNPVVSLPEMTKKAIDTLS 252 +NY FA +++ + E N + L +++ L+ Sbjct: 183 LDNFNYFDRKRIFGL---------FAEDDLERTEFEP-----NLLEMLDFSIRESKRLLA 228 Query: 253 TNQKGFFLMVEEEGTDEFAHKNNAKMTIKSGQALDASVQVAKDFAKKNPDTLVLVLADHE 312 KGFF+M E D +H N+ + +K Q + +V+ A +FAK+N DTLV+V ADHE Sbjct: 229 DGCKGFFIMAEGSKIDWASHDNDYEYYLKEMQEFEKTVERALNFAKENNDTLVIVTADHE 288 Query: 313 TGGLSIEPVDAEDETGDGISQEDGPFAIANSKESFVVDWTTS----GHTAVDIPVTAMGK 368 TGGL IE DA +E G I+ W T+ HT + IPV A G Sbjct: 289 TGGLLIEQDDAR-------YRETGKMKIS---------WNTAIGRGAHTGIMIPVFAQGP 332 Query: 369 NAQLFTGVFENTAVFDKLMQAYGFN 393 A+ F+GV +NT +F + +A G + Sbjct: 333 GAENFSGVMDNTDIFFAIKEALGID 357 >gb|EAJ14886.1| unknown [environmental sequence] Length = 403 Score = 171 bits (434), Expect = 2e-41, Method: Compositional matrix adjust. Identities = 127/395 (32%), Positives = 190/395 (48%), Gaps = 32/395 (8%) Query: 7 KPAQAASSKTKNVILFVGDGMGTAARNAIRLATVGEKGKLAMDDMPYAGLVHTSST-VPV 65 K +K KN+IL + DGM + A R+ +++MD PY+G+V T S V Sbjct: 30 KELSYVDNKAKNIILLIADGMSLSQITAYRMMKGSLNERISMDKFPYSGIVLTHSYDAAV 89 Query: 66 TDXXXXXXXXXXGVKTYNGAIGMDANKKSVKTIAEYAKEAGKSTGVVTTSQITDATGAAF 125 TD G KT NGA+G+D + ++ + E + G + +++TS++T AT AAF Sbjct: 90 TDSASSATAYSTGFKTNNGALGLDQDLNELENLTETIDKFGFVSSLISTSEVTHATPAAF 149 Query: 126 GAHVEDRSKQSDIALQLLTKSKVDVLLGGGEDFWYPAGEAGKFPDAPAEDPSEKSKGTQG 185 AHV+ R K I+ Q++ S+V +LGGG F+ P + GK D K Sbjct: 150 AAHVDLRWKTDIISSQMI-DSRVATILGGGRYFFTPEEQGGKREDGRNLLDEIKESHI-- 206 Query: 186 NLVTKAKQLGYNYVSTXXXXXXXXXXXXXXXFANEEMFQQNEEGKGDIYNPVVSLPEMTK 245 L+T+ QL FA+E + +E K D ++ SL EM + Sbjct: 207 -LLTEKNQLS--------EISSSLDKQVLGLFADEHL---RDEDKKDNHDLEPSLAEMLQ 254 Query: 246 KAI--DTLSTNQ--KGFFLMVEEEGTDEFAHKNNAKMTIKSGQALDASVQVAKDFAKKNP 301 +I L NQ +GFF+M E D H N+ I+ + D +V +A + AK+ Sbjct: 255 YSITRSDLLMNQGCEGFFIMAEGSQVDWAGHVNDFDYLIREMEDFDEAVDLALEIAKERQ 314 Query: 302 DTLVLVLADHETGGLSIEPVDAEDETGDGISQEDGPFAIANSKESFVVDWTTSGHTAVDI 361 DTLVLV +DHETGGL IEP + D + D + K SF + HT V + Sbjct: 315 DTLVLVTSDHETGGLLIEPANPIDNSLDEV------------KFSFNTAVGSGTHTGVPV 362 Query: 362 PVTAMGKNAQLFTGVFENTAVFDKLMQAYGFNAKK 396 PV A G ++ FTG +NT ++ ++ A + KK Sbjct: 363 PVYAYGPGSENFTGTLDNTDIYYAMLAALDLDDKK 397 >gb|EAH99728.1| unknown [environmental sequence] Length = 392 Score = 166 bits (419), Expect = 1e-39, Method: Compositional matrix adjust. Identities = 120/378 (31%), Positives = 186/378 (49%), Gaps = 34/378 (8%) Query: 18 NVILFVGDGMGTAARNAIRLATVGEKGKLAMDDMPYAG--LVHTSSTVPVTDXXXXXXXX 75 N+ILF+GDGM + A+RL+ G ++A+D+ PY+G L H+++ + VTD Sbjct: 31 NIILFIGDGMSMSQITALRLSQGGPNSRVAVDEFPYSGKVLTHSANAI-VTDSASSGTAF 89 Query: 76 XXGVKTYNGAIGMDANKKSVKTIAEYAKEAGKSTGVVTTSQITDATGAAFGAHVEDRSKQ 135 G+KT N A+G+D + V+ I E ++G + V++TS++T AT AAF AH R Sbjct: 90 STGIKTNNRALGVDVSGNPVENITEILDKSGFVSSVISTSEVTHATPAAFVAHASSRYLS 149 Query: 136 SDIALQLLTKSKVDVLLGGGEDFWYPAGEAGKFPDAPAEDPSEKSKGTQGNLVTKAKQLG 195 +I++Q+ + S + +LGGG F+ + G D + EK K L+T +L Sbjct: 150 DEISIQM-SNSNIKNILGGGRKFFMTEEDGGIREDGI--NLIEKVKANS-KLITHKDEL- 204 Query: 196 YNYVSTXXXXXXXXXXXXXXXFANEEMFQQNEEGKGDIYNPVVSLPEMTKKAIDT----L 251 FA+E + N + I P S+ EM + +I+ + Sbjct: 205 -------EDIAQYGSEQVFGLFADEHLRDTNTP-ENHITEP--SIKEMLEFSINRSKNFI 254 Query: 252 STNQKGFFLMVEEEGTDEFAHKNNAKMTIKSGQALDASVQVAKDFAKKNPDTLVLVLADH 311 GFF+M E D H N+ + ++ L+ ++ A +FAKKN DTLV+V ADH Sbjct: 255 KNGCNGFFVMAEASQIDWAGHGNDYEYLMREMLDLEEGIKWALEFAKKNDDTLVIVTADH 314 Query: 312 ETGGLSIEPVDAEDETGDGISQEDGPFAIANSKESFVVDWTTSGHTAVDIPVTAMGKNAQ 371 ETGGL IEP D + + K SF HT + +P+ AMG ++ Sbjct: 315 ETGGLLIEPSDPR------------KYENLDVKFSFNTGIGRGSHTGIPVPIFAMGPGSE 362 Query: 372 LFTGVFENTAVFDKLMQA 389 FTG +NT V L++A Sbjct: 363 NFTGTLDNTDVHKALLEA 380 >gb|EAJ74642.1| unknown [environmental sequence] Length = 403 Score = 164 bits (414), Expect = 4e-39, Method: Compositional matrix adjust. Identities = 120/385 (31%), Positives = 188/385 (48%), Gaps = 28/385 (7%) Query: 14 SKTKNVILFVGDGMGTAARNAIRLATVGEKGKLAMDDMPYAGLVHTSST-VPVTDXXXXX 72 +K KN+IL + DGM ++ RL G ++A+D P +G+V T S VTD Sbjct: 38 NKAKNIILLISDGMSLTQVSSYRLLKGGPNERIAVDTFPVSGIVLTHSKDAIVTDSASSA 97 Query: 73 XXXXXGVKTYNGAIGMDANKKSVKTIAEYAKEAGKSTGVVTTSQITDATGAAFGAHVEDR 132 G KT NGA+G+D + + ++ E + G + +++TS+IT AT AA+ +HV+ R Sbjct: 98 TAFSTGKKTNNGALGLDQDNQILENFTEKIDKFGYVSSLISTSEITHATPAAYASHVDLR 157 Query: 133 SKQSDIALQLLTKSKVDVLLGGGEDFWYPAGEAGKFPDAPAEDPSEKSKGTQGNLVTKAK 192 K +I+LQ++ S V +LGGG F+ P GK D + E+ + + L K++ Sbjct: 158 WKTDEISLQMM-NSDVMTILGGGRHFFIPEEMGGKRADEV--NLLEEIESSHTILTKKSE 214 Query: 193 QLGYNYVSTXXXXXXXXXXXXXXXFANEEMFQQNEEGKGDIYNPVVS-LPEMTKKAIDTL 251 ++Y + FA+E + + E+ P S + E K + Sbjct: 215 MDSFDYSNNGKVVGL---------FADEAL-RDAEKPDNHSLEPTSSEMLEFALKRSEQF 264 Query: 252 STN-QKGFFLMVEEEGTDEFAHKNNAKMTIKSGQALDASVQVAKDFAKKNPDTLVLVLAD 310 ++N KGFF+MVE D H NN + Q D +V+ A +FAK N +TLV+ AD Sbjct: 265 TSNGCKGFFVMVEGSQVDWAGHANNLDYLKREMQDFDEAVKTALEFAKSNQETLVIATAD 324 Query: 311 HETGGLSIEPVDAEDETGDGISQEDGPFAIANSKESFVVDWTTSGHTAVDIPVTAMGKNA 370 HETGGL IEP + T + K SF HT V +PV A G + Sbjct: 325 HETGGLLIEPATPTNYTSPEV------------KFSFNTGIGYGSHTGVPVPVYAYGPGS 372 Query: 371 QLFTGVFENTAVFDKLMQAYGFNAK 395 + FTG +NT ++ +++A ++ Sbjct: 373 ENFTGTLDNTDIYYAMVEALDLTSR 397 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.310 0.127 0.353 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 540,418,352 Number of extensions: 21427090 Number of successful extensions: 43459 Number of sequences better than 1.0e-05: 310 Number of HSP's gapped: 42886 Number of HSP's successfully gapped: 401 Length of query: 396 Length of database: 757,237,578 Length adjustment: 128 Effective length of query: 268 Effective length of database: 409,704,906 Effective search space: 109800914808 Effective search space used: 109800914808 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.7 bits) S2: 127 (53.5 bits)