BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0562.0007.p (458 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_625484.1| putative export protein [Streptomyces coelicolo... 314 3e-84 gb|AAB07864.1| membrane-bound transport protein >gi|2347008|dbj|... 280 5e-74 ref|ZP_00097277.1| COG0477: Permeases of the major facilitator s... 266 8e-70 ref|NP_786406.1| transport protein [Lactobacillus plantarum WCFS... 265 2e-69 ref|NP_266765.1| membrane-bound transport protein [Lactococcus l... 243 9e-63 >ref|NP_625484.1| putative export protein [Streptomyces coelicolor A3(2)] emb|CAB61606.1| putative export protein [Streptomyces coelicolor A3(2)] Length = 496 Score = 314 bits (804), Expect = 3e-84, Method: Compositional matrix adjust. Identities = 175/444 (39%), Positives = 250/444 (56%), Gaps = 4/444 (0%) Query: 11 QKQSSETFEAVEQQAAAKKMMWILMVGMLAPLFDTTITNVAIDTLVREFSTSVSTIQWVM 70 +S A +++ L+VG L +FDTT+ +VA+D L + F ++T+QWV Sbjct: 5 HGAASAAARPDRVDPAVRRIGATLVVGALGVVFDTTVVSVALDDLAKAFDAPLATVQWVS 64 Query: 71 TSYLLALGMVIPXXXXXXXXXGGKPMWLFSLGMFLLGSVLCSLAWNVESLIFFRTIQGIG 130 T YLLA+ + IP GG+ +W+ +LG FLLGSVL +LAWN SLI FR +QG+ Sbjct: 65 TGYLLAVFVTIPLAVWAQARFGGRRLWIAALGGFLLGSVLSALAWNATSLIVFRVVQGLA 124 Query: 131 GGLLMPIMQTLGVRAFKGENMGKMMATXXXXXXXXXXXXXXXXXXXXNHLSWRWIFLVNI 190 GG++MP+M TL ++A +G + G++MA + WRW+F VNI Sbjct: 125 GGIMMPLMATLLMQAARGSHPGRVMAIITVPTALGPILGPVLGGLVLHLADWRWMFFVNI 184 Query: 191 PICIVAIIMAWRGLPNE--ERGTRTLRLDLPGLMLLSPAIVLIIYGLGEVSSNHGFGHSX 248 P C+V +A R LP++ E G RLD+ GL+LL P + ++YGL V G + Sbjct: 185 PFCVVGGWLALRNLPDDRPEPGGPRGRLDVVGLLLLCPGVAALVYGLSRVEGGAGTTGAA 244 Query: 249 XXXXXXXXXXXXXXXXXXXFRKGKEALLDIRLFRVRSLAVSSILLFLSGLTTYGAMLLLP 308 + AL+D+RLFR R++A S+ LLFL G+ YG M+LLP Sbjct: 245 VLVPLLGGLALVGGFTARSLTRPGGALIDLRLFRHRAVASSAALLFLGGIALYGPMMLLP 304 Query: 309 LYFQQVRGESVLISGLLLVPQGIGMLLTRSLAGKLTDQIGSRPVVXXXXXXXXXXXWPFT 368 LYFQQVRGE L +GL L+PQG+G LL R+LAG+ D++G R V PF Sbjct: 305 LYFQQVRGEDALGAGLALIPQGVGALLARALAGRYMDRVGPRRVAVTAFALVAVATVPFA 364 Query: 369 QLGADTSYYYLGAALIVRGAGLGAVFLPVMASAYIGLSSQQIPHASSTTRIMQQIGGAFG 428 + A T L AAL VRG LGA + M +AY+GL Q+IP A TR+ QQIGG+ G Sbjct: 365 FVTAGTGGAVLMAALFVRGIALGAAMIAPMGAAYVGLRHQEIPDAGVITRVAQQIGGSVG 424 Query: 429 ASVIAIILQNEYNSVMSQNPAAIA 452 +++A++LQ + + + P+A+A Sbjct: 425 IALLAVVLQ--HAAAGAHGPSALA 446 >gb|AAB07864.1| membrane-bound transport protein dbj|BAA21912.1| hemX [Propionibacterium freudenreichii] Length = 487 Score = 280 bits (716), Expect = 5e-74, Method: Compositional matrix adjust. Identities = 157/421 (37%), Positives = 228/421 (54%), Gaps = 2/421 (0%) Query: 29 KMMWILMVGMLAPLFDTTITNVAIDTLVREFSTSVSTIQWVMTSYLLALGMVIPXXXXXX 88 +++ L+VG + + DTTI + + TL VST+QWV T YLLAL + IP Sbjct: 17 RLLVALVVGGITAIMDTTIVAIGMHTLTEALHAPVSTLQWVSTGYLLALAVAIPFVSWAQ 76 Query: 89 XXXGGKPMWLFSLGMFLLGSVLCSLAWNVESLIFFRTIQGIGGGLLMPIMQTLGVRAFKG 148 GGK +WLF+LG+F + S LC+L+W+ SLI FR +QG GGG++ P+MQTL ++ Sbjct: 77 TRFGGKRLWLFALGLFTVSSALCALSWSAASLIAFRVLQGFGGGIMFPLMQTLVMQHVDR 136 Query: 149 ENMGKMMATXXXXXXXXXXXXXXXXXXXXNHLSWRWIFLVNIPICIVAIIMAWRGLPNE- 207 M + MA N LSW W+FL+N+PI +V ++A + ++ Sbjct: 137 RGMTRAMANVSLPIALGPIIGPVLGGVVLNWLSWHWLFLINVPIGVVGWVLAMAFITDDR 196 Query: 208 -ERGTRTLRLDLPGLMLLSPAIVLIIYGLGEVSSNHGFGHSXXXXXXXXXXXXXXXXXXX 266 RLDL G +LLS A+ ++YGL GF + Sbjct: 197 PRPAAGHPRLDLVGAVLLSLALAGMLYGLSNAYDVGGFHRADVLVPALVGLALLAGFVAW 256 Query: 267 XFRKGKEALLDIRLFRVRSLAVSSILLFLSGLTTYGAMLLLPLYFQQVRGESVLISGLLL 326 R+G AL+D+RL VRS+ VSS+ L L G T + A LLPLYFQ +RG L + LLL Sbjct: 257 ARRRGSRALIDVRLLAVRSVRVSSVTLTLVGATLFSANFLLPLYFQSLRGYDALNAALLL 316 Query: 327 VPQGIGMLLTRSLAGKLTDQIGSRPVVXXXXXXXXXXXWPFTQLGADTSYYYLGAALIVR 386 +PQGIG LL+R + L + G R PF G DT + LG L VR Sbjct: 317 IPQGIGSLLSRFIVSSLVARFGPRLTAVAGLLISAAATVPFALAGTDTGLWLLGTVLFVR 376 Query: 387 GAGLGAVFLPVMASAYIGLSSQQIPHASSTTRIMQQIGGAFGASVIAIILQNEYNSVMSQ 446 G G+G V +P+M AY+ ++ + +PHAS+ TRI+QQ+GGAFG +++A+ L + ++ + Sbjct: 377 GFGMGVVLIPIMTGAYVDIAREHMPHASAITRIVQQLGGAFGTALVAVALTSRASTARPR 436 Query: 447 N 447 + Sbjct: 437 D 437 >ref|ZP_00097277.1| COG0477: Permeases of the major facilitator superfamily [Desulfitobacterium hafniense] Length = 473 Score = 266 bits (680), Expect = 8e-70, Method: Compositional matrix adjust. Identities = 153/419 (36%), Positives = 232/419 (55%), Gaps = 5/419 (1%) Query: 19 EAVEQQAAAKKMM---WILMVGMLAPLFDTTITNVAIDTLVREFSTSVSTIQWVMTSYLL 75 +A E + K +M WIL++G + PL D+T+ N+AI+ L +FS + IQWV+T Y+L Sbjct: 7 KAPELEPIPKNIMNIAWILVLGAIMPLLDSTMVNIAINHLSHDFSIGLDLIQWVITGYVL 66 Query: 76 ALGMVIPXXXXXXXXXGGKPMWLFSLGMFLLGSVLCSLAWNVESLIFFRTIQGIGGGLLM 135 A+ + +P GK + + + +FL S+ +W++ SLI FR +QG G +M Sbjct: 67 AMAISVPLAGWMVQRFNGKWLMIGANILFLAASIASGFSWSIHSLIIFRVVQGFSAGFIM 126 Query: 136 PIMQTLGVRAFKGENMGKMMATXXXXXXXXXXXXXXXXXXXXNHLSWRWIFLVNIPICIV 195 ++ TL V E MG++M+T LSWR+IF VNIP+ I+ Sbjct: 127 TLVTTLCVETAGRERMGRLMSTIGIPTVLGPILGPVIGAVIVQFLSWRYIFFVNIPLGIL 186 Query: 196 AI-IMAWRGLPNEERGTRTLRLDLPGLMLLSPAIVLIIYGLGEVSSNHGFGHSXXXXXXX 254 AI +M W+ LP+ + D G++LL A +IYG+ + + + F +S Sbjct: 187 AITLMIWK-LPDFTPANIKAKFDFIGIILLGTASAALIYGITKAAKSATFNNSTTIAYGV 245 Query: 255 XXXXXXXXXXXXXFRKGKEALLDIRLFRVRSLAVSSILLFLSGLTTYGAMLLLPLYFQQV 314 K +A+L + LFR+++ + + LFL+G+ T G MLLLPL+FQ + Sbjct: 246 AGIAILAIYMIYAALKKDQAILPLHLFRLKNFSAVMVGLFLAGIATNGPMLLLPLFFQNI 305 Query: 315 RGESVLISGLLLVPQGIGMLLTRSLAGKLTDQIGSRPVVXXXXXXXXXXXWPFTQLGADT 374 +G SVL +GL+L+PQGIGML+ R L GKLTD+ G+R VV PF + + Sbjct: 306 KGFSVLNTGLILIPQGIGMLIARPLIGKLTDKWGARNVVLVSLALTIVGTIPFIFINEAS 365 Query: 375 SYYYLGAALIVRGAGLGAVFLPVMASAYIGLSSQQIPHASSTTRIMQQIGGAFGASVIA 433 S + + L VRG G+G V +P+M AY G+ Q+I AS TR+MQ IGGAFG++V+A Sbjct: 366 SLFVVSLVLFVRGVGIGGVTIPMMTDAYTGMVKQEIAQASVGTRLMQNIGGAFGSAVMA 424 >ref|NP_786406.1| transport protein [Lactobacillus plantarum WCFS1] emb|CAD65268.1| transport protein [Lactobacillus plantarum WCFS1] Length = 492 Score = 265 bits (676), Expect = 2e-69, Method: Compositional matrix adjust. Identities = 149/411 (36%), Positives = 226/411 (54%) Query: 26 AAKKMMWILMVGMLAPLFDTTITNVAIDTLVREFSTSVSTIQWVMTSYLLALGMVIPXXX 85 A + W+++ G +APL D+T+ N+AI+ LV F +SV+T+QW +T YLLA G+ +P Sbjct: 10 ATMTIAWVVVFGAMAPLLDSTMINIAINNLVTSFHSSVTTVQWAVTGYLLATGVAVPFSS 69 Query: 86 XXXXXXGGKPMWLFSLGMFLLGSVLCSLAWNVESLIFFRTIQGIGGGLLMPIMQTLGVRA 145 GK +++ +F LGS+ ++A N++ LI R +QG GGL+MP++ TL V+ Sbjct: 70 WLLNRFDGKAVFVAGEILFGLGSIFAAVAPNIQLLIIARLVQGFAGGLIMPLLTTLLVQT 129 Query: 146 FKGENMGKMMATXXXXXXXXXXXXXXXXXXXXNHLSWRWIFLVNIPICIVAIIMAWRGLP 205 MG+MMAT SW WIF +N+P+ +++I + +P Sbjct: 130 AGAAVMGQMMATVGLPMILGPLIGPVIGGIIIKFASWHWIFWINVPVVLISIALILWKMP 189 Query: 206 NEERGTRTLRLDLPGLMLLSPAIVLIIYGLGEVSSNHGFGHSXXXXXXXXXXXXXXXXXX 265 N RT ++D G++LL + IIY L + F ++ Sbjct: 190 NYPAQNRTAKMDFIGILLLIVSSSAIIYSLVNAAHTDRFNNATSIEMLILGAIALISYVV 249 Query: 266 XXFRKGKEALLDIRLFRVRSLAVSSILLFLSGLTTYGAMLLLPLYFQQVRGESVLISGLL 325 + + A++ + LF+ S + + LF++G GAMLLLPLYFQ VRG SV+ + L Sbjct: 250 WAAWQKERAVVPLHLFKFASFDGAGLGLFIAGTVLNGAMLLLPLYFQNVRGMSVINAALA 309 Query: 326 LVPQGIGMLLTRSLAGKLTDQIGSRPVVXXXXXXXXXXXWPFTQLGADTSYYYLGAALIV 385 L+PQG GML++R+L G+LTDQIG++ VV PF G TSY+ L L V Sbjct: 310 LLPQGAGMLVSRTLTGRLTDQIGAKYVVLGSVVITFVGTLPFYWFGQQTSYWILALILFV 369 Query: 386 RGAGLGAVFLPVMASAYIGLSSQQIPHASSTTRIMQQIGGAFGASVIAIIL 436 RG G G + +P+MA AY G+ Q+P A+ TRI+Q IG AFG+++I I+ Sbjct: 370 RGIGAGGILMPLMADAYTGMQPGQVPAATIGTRIIQNIGSAFGSALITAIV 420 >ref|NP_266765.1| membrane-bound transport protein [Lactococcus lactis subsp. lactis] pir||A86701 membrane-bound transport protein yfjF [imported] - Lactococcus lactis subsp. lactis (strain IL1403) gb|AAK04707.1| membrane-bound transport protein [Lactococcus lactis subsp. lactis] Length = 501 Score = 243 bits (619), Expect = 9e-63, Method: Compositional matrix adjust. Identities = 138/419 (32%), Positives = 230/419 (54%), Gaps = 4/419 (0%) Query: 22 EQQAAAKKMM---WILMVGMLAPLFDTTITNVAIDTLVREFSTSVSTIQWVMTSYLLALG 78 E++ K++ WIL+VG +AP+ D+T+ N+AI+ L + TS+ IQW +T Y+LAL Sbjct: 8 EKEKLPKELTRNAWILVVGAIAPMLDSTMVNIAINKLQSDLHTSLDMIQWAITGYVLALA 67 Query: 79 MVIPXXXXXXXXXGGKPMWLFSLGMFLLGSVLCSLAWNVESLIFFRTIQGIGGGLLMPIM 138 + +P GK + +SL F L S L +AWN++S I FR +QG G + +M Sbjct: 68 VAVPVCGYVVNHIDGKKVMQWSLLSFGLFSFLSGIAWNIDSFIIFRAVQGFSAGFITLLM 127 Query: 139 QTLGVRAFKGENMGKMMATXXXXXXXXXXXXXXXXXXXXNHLSWRWIFLVNIPICIVAII 198 TL ++ + +G++MA + +W WIF +NIP+ I+A++ Sbjct: 128 TTLLMQITPPDKLGQLMAVVSTPIILGPIIGPVLGGAIVSGANWHWIFYINIPVVIIAVL 187 Query: 199 MAWRGLPNEERGTRTLRLDLPGLMLLSPAIVLIIYGLGEVSSN-HGFGHSXXXXXXXXXX 257 + L + + +LD G +LL+ V +IYG+ + S+N F +S Sbjct: 188 LNHFYLSSFKPFNPKAKLDWFGTLLLAGGSVGLIYGMMKGSTNAKNFFNSQMITFVIIGL 247 Query: 258 XXXXXXXXXXFRKGKEALLDIRLFRVRSLAVSSILLFLSGLTTYGAMLLLPLYFQQVRGE 317 + +L +R F+ ++ ++I +FL+G+ + G MLLLPL+FQ +G Sbjct: 248 ALFVIYGIYNRIRKNNTVLPLRFFKSKNFTAANIGIFLAGIASNGPMLLLPLFFQNTKGF 307 Query: 318 SVLISGLLLVPQGIGMLLTRSLAGKLTDQIGSRPVVXXXXXXXXXXXWPFTQLGADTSYY 377 + + +GL+L+PQG+GM++TR L GK+ D++G+RPVV PF + +T+ Sbjct: 308 TAVEAGLMLIPQGLGMMVTRPLIGKMIDRMGARPVVLVSLAISLLGTLPFIFVHENTNLI 367 Query: 378 YLGAALIVRGAGLGAVFLPVMASAYIGLSSQQIPHASSTTRIMQQIGGAFGASVIAIIL 436 +LG L VRG G+G + +P+M +IGL Q IP AS RI+Q +G +FG++VI+ ++ Sbjct: 368 WLGLVLFVRGMGIGGLQMPMMTDIFIGLDKQDIPGASVGQRIIQNVGSSFGSAVISAVV 426 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.326 0.139 0.414 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 522,235,216 Number of extensions: 18006024 Number of successful extensions: 64846 Number of sequences better than 1.0e-05: 983 Number of HSP's gapped: 63303 Number of HSP's successfully gapped: 1105 Length of query: 458 Length of database: 757,237,578 Length adjustment: 129 Effective length of query: 329 Effective length of database: 406,989,807 Effective search space: 133899646503 Effective search space used: 133899646503 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 128 (53.9 bits)